Restriction map for
pFN21AE2369
(Linear) (Six-base) MAPSORT of: KIEE2369.seq Check: 4662 from: 1 to: 1740
KIEE2369.orf
With 182 enzymes: SgfI *
August 18, 2011 15:44 ..
Acc65I G'GTAC_C
Cuts at: 0 1004 1095 1740
Size: 1004 91 645
Fragments arranged by size:
1004 645 91
AflII C'TTAA_G
Cuts at: 0 792 1740
Size: 792 948
AflIII A'CryG_T
Cuts at: 0 895 1215 1740
Size: 895 320 525
Fragments arranged by size:
895 525 320
AhdI GACnn_n'nnGTC
Cuts at: 0 489 1740
Size: 489 1251
AleI CACnn'nnGTG
Cuts at: 0 1438 1740
Size: 1438 302
AlwNI CAG_nnn'CTG
Cuts at: 0 99 157 937 1740
Size: 99 58 780 803
Fragments arranged by size:
803 780 99 58
ApaLI G'TGCA_C
Cuts at: 0 1219 1740
Size: 1219 521
ApoI r'AATT_y
Cuts at: 0 1363 1740
Size: 1363 377
AscI GG'CGCG_CC
Cuts at: 0 375 1740
Size: 375 1365
AvaI C'yCGr_G
Cuts at: 0 193 214 1740
Size: 193 21 1526
Fragments arranged by size:
1526 193 21
BanI G'GyrC_C
Cuts at: 0 319 475 1004 1095 1740
Size: 319 156 529 91 645
Fragments arranged by size:
645 529 319 156 91
BanII G_rGCy'C
Cuts at: 0 1583 1740
Size: 1583 157
BbeI G_GCGC'C
Cuts at: 0 479 1740
Size: 479 1261
BbsI GAAGACnn'nnnn_
Cuts at: 0 916 1511 1740
Size: 916 595 229
Fragments arranged by size:
916 595 229
BbvCI CC'TCA_GC
Cuts at: 0 186 1740
Size: 186 1554
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 525 559 1740
Size: 525 34 1181
Fragments arranged by size:
1181 525 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1230 1740
Size: 1230 510
BclI T'GATC_A
Cuts at: 0 554 1011 1740
Size: 554 457 729
Fragments arranged by size:
729 554 457
BlpI GC'TnA_GC
Cuts at: 0 40 403 1667 1740
Size: 40 363 1264 73
Fragments arranged by size:
1264 363 73 40
Bme1580I G_kGCm'C
Cuts at: 0 305 1223 1740
Size: 305 918 517
Fragments arranged by size:
918 517 305
BmgBI CAC'GTC
Cuts at: 0 1653 1740
Size: 1653 87
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 99 1740
Size: 99 1641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 636 1740
Size: 293 343 1104
Fragments arranged by size:
1104 343 293
Bpu10I CC'TnA_GC
Cuts at: 0 186 504 1740
Size: 186 318 1236
Fragments arranged by size:
1236 318 186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 1740
Size: 69 1671
BsaI GGTCTCn'nnnn_
Cuts at: 0 451 1740
Size: 451 1289
BsaAI yAC'GTr
Cuts at: 0 1218 1740
Size: 1218 522
BsaHI Gr'CG_yC
Cuts at: 0 26 476 490 1740
Size: 26 450 14 1250
Fragments arranged by size:
1250 450 26 14
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 211 241 1740
Size: 211 30 1499
Fragments arranged by size:
1499 211 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 137 290 843 1740
Size: 137 153 553 897
Fragments arranged by size:
897 553 153 137
BseYI C'CCAG_C
Cuts at: 0 363 1740
Size: 363 1377
BsiEI CG_ry'CG
Cuts at: 0 37 1740
Size: 37 1703
BsiHKAI G_wGCw'C
Cuts at: 0 221 873 1223 1740
Size: 221 652 350 517
Fragments arranged by size:
652 517 350 221
Bsp1286I G_dGCh'C
Cuts at: 0 221 305 873 1223 1583 1740
Size: 221 84 568 350 360 157
Fragments arranged by size:
568 360 350 221 157 84
BspHI T'CATG_A
Cuts at: 0 1229 1740
Size: 1229 511
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2 1740
Size: 2 1738
BsrBI CCG'CTC
Cuts at: 0 39 299 1671 1740
Size: 39 260 1372 69
Fragments arranged by size:
1372 260 69 39
BsrFI r'CCGG_y
Cuts at: 0 253 371 1740
Size: 253 118 1369
Fragments arranged by size:
1369 253 118
BssHII G'CGCG_C
Cuts at: 0 375 1740
Size: 375 1365
BssSI C'ACGA_G
Cuts at: 0 73 1740
Size: 73 1667
BstAPI GCAn_nnn'nTGC
Cuts at: 0 99 157 1740
Size: 99 58 1583
Fragments arranged by size:
1583 99 58
BstBI TT'CG_AA
Cuts at: 0 386 1740
Size: 386 1354
BstXI CCAn_nnnn'nTGG
Cuts at: 0 237 1740
Size: 237 1503
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1060 1740
Size: 1060 680
BtsI GCAGTG_nn'
Cuts at: 0 321 1740
Size: 321 1419
DrdI GACnn_nn'nnGTC
Cuts at: 0 465 1651 1740
Size: 465 1186 89
Fragments arranged by size:
1186 465 89
EaeI y'GGCC_r
Cuts at: 0 34 1740
Size: 34 1706
EagI C'GGCC_G
Cuts at: 0 34 1740
Size: 34 1706
EarI CTCTTCn'nnn_
Cuts at: 0 1510 1710 1740
Size: 1510 200 30
Fragments arranged by size:
1510 200 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 275 1740
Size: 275 1465
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 636 1740
Size: 293 343 1104
Fragments arranged by size:
1104 343 293
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1686 1740
Size: 1686 54
EcoRV GAT'ATC
Cuts at: 0 1058 1740
Size: 1058 682
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 903 1740
Size: 903 837
HaeII r_GCGC'y
Cuts at: 0 244 479 1740
Size: 244 235 1261
Fragments arranged by size:
1261 244 235
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 475 507 838 870 1224 1256 1740
Size: 475 32 331 32 354 32 484
Fragments arranged by size:
484 475 354 331 32 32 32
KasI G'GCGC_C
Cuts at: 0 475 1740
Size: 475 1265
KpnI G_GTAC'C
Cuts at: 0 1008 1099 1740
Size: 1008 91 641
Fragments arranged by size:
1008 641 91
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 254 1740
Size: 99 155 1486
Fragments arranged by size:
1486 155 99
MslI CAynn'nnrTG
Cuts at: 0 708 1438 1740
Size: 708 730 302
Fragments arranged by size:
730 708 302
MspA1I CmG'CkG
Cuts at: 0 8 157 175 757 1245 1270 1740
Size: 8 149 18 582 488 25 470
Fragments arranged by size:
582 488 470 149 25 18 8
NarI GG'CG_CC
Cuts at: 0 476 1740
Size: 476 1264
NotI GC'GGCC_GC
Cuts at: 0 34 1740
Size: 34 1706
NspI r_CATG'y
Cuts at: 0 432 1740
Size: 432 1308
PasI CC'CwG_GG
Cuts at: 0 1576 1740
Size: 1576 164
PflMI CCAn_nnn'nTGG
Cuts at: 0 78 1740
Size: 78 1662
PfoI T'CCnGG_A
Cuts at: 0 1687 1740
Size: 1687 53
PmlI CAC'GTG
Cuts at: 0 1218 1740
Size: 1218 522
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 340 372 1740
Size: 340 32 1368
Fragments arranged by size:
1368 340 32
PstI C_TGCA'G
Cuts at: 0 13 104 153 1740
Size: 13 91 49 1587
Fragments arranged by size:
1587 91 49 13
PvuII CAG'CTG
Cuts at: 0 8 1245 1270 1740
Size: 8 1237 25 470
Fragments arranged by size:
1237 470 25 8
SfcI C'TryA_G
Cuts at: 0 9 100 149 1175 1740
Size: 9 91 49 1026 565
Fragments arranged by size:
1026 565 91 49 9
SfoI GGC'GCC
Cuts at: 0 477 1740
Size: 477 1263
SgrAI Cr'CCGG_yG
Cuts at: 0 371 1740
Size: 371 1369
SmlI C'TyrA_G
Cuts at: 0 48 792 1740
Size: 48 744 948
Fragments arranged by size:
948 744 48
StyI C'CwwG_G
Cuts at: 0 1038 1740
Size: 1038 702
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 475 538 1135 1740
Size: 475 63 597 605
Fragments arranged by size:
605 597 475 63
TatI w'GTAC_w
Cuts at: 0 939 1278 1740
Size: 939 339 462
Fragments arranged by size:
939 462 339
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 211 243 1740
Size: 211 32 1497
Fragments arranged by size:
1497 211 32
XbaI T'CTAG_A
Cuts at: 0 856 1740
Size: 856 884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 759 1573 1740
Size: 759 814 167
Fragments arranged by size:
814 759 167
Enzymes that do cut:
Acc65I AflII AflIII AhdI AleI AlwNI ApaLI ApoI
AscI AvaI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BlpI Bme1580I BmgBI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstXI BtgZI BtsI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRV FalI HaeII Hin4I
KasI KpnI MmeI MslI MspA1I NarI NotI NspI
PasI PflMI PfoI PmlI PsrI PstI PvuII SfcI
SfoI SgrAI SmlI StyI TaqII TatI TstI XbaI
XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AgeI AlfI
AloI ApaI AseI AsiSI AvrII BaeI BamHI BfrBI
BglI BglII BmrI BmtI BsaBI BsaWI BsgI BsiWI
BsmI BsmBI BspEI BsrDI BsrGI BstEII BstYI BstZ17I
Bsu36I BtgI ClaI CspCI DraI DraIII EcoICRI EcoO109I
EcoRI FseI FspI FspAI HincII HindIII HpaI MfeI
MluI MscI NaeI NcoI NdeI NgoMIV NheI NruI
NsiI PacI PciI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XhoI
XmaI XmnI ZraI