################################## # NO CUT ENZYMES ( 189 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HindIII HinfI HphI KasI KpnI KspI MaeI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI NarI NciI NcoI NdeI NdeII NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI TspRI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 270 CGCG CG^CG AciI 260 CCGC CCGC(-3/-1) AcyI 443 GGCGTC G[GA]^CG[CT]C AluI 318 AGCT AG^CT Asp700I 45 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 170 GGACC G^G[AT]CC BanI 326 GGTGCC G^G[CT][GA]CC BmyI 327 GTGCCC G[AGT]GC[ACT]^C BsaHI 443 GGCGTC G[GA]^CG[CT]C BsaJI 330 CCCAGG C^C[ACGT][ACGT]GG BseRI 378 GAGGAG GAGGAG(10/8) BsiYI 256 CCCACCGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 256 CCCACCGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 199 GTCTC GTCTC(1/5) BsmBI 198 CGTCTC CGTCTC(1/5) BsmFI 169 GGGAC GGGAC(10/14) Bsp1286I 327 GTGCCC G[AGT]GC[ACT]^C BsrFI 262 GCCGGC [GA]^CCGG[CT] BssKI 331 CCAGG ^CC[ACGT]GG BstNI 331 CCAGG CC^[AT]GG BstUI 270 CGCG CG^CG CfoI 269 GCGC GCG^C Cfr10I 262 GCCGGC [GA]^CCGG[CT] Cfr13I 170 GGACC G^G[ACGT]CC EaeI 214 CGGCCA [CT]^GGCC[GA] EarI 375 GAAGAG CTCTTC(1/4) EcoRII 331 CCAGG ^CC[AT]GG HaeIII 215 GGCC GG^CC HapII 263 CCGG C^CGG HhaI 269 GCGC GCG^C Hin1I 443 GGCGTC G[GA]^CG[CT]C HinP1I 269 GCGC G^CGC HincII 78 GTTAAC GT[CT]^[GA]AC HindII 78 GTTAAC GT[CT]^[GA]AC HpaI 78 GTTAAC GTT^AAC HpaII 263 CCGG C^CGG Ksp632I 375 GAAGAG CTCTTC(1/4) MnlI 378 GAGG CCTC(7/6) MspI 263 CCGG C^CGG MvaI 331 CCAGG CC^[AT]GG MvnI 270 CGCG CG^CG NaeI 262 GCCGGC GCC^GGC NgoMIV 262 GCCGGC G^CCGGC NlaIII 297 CATG CATG^ Sau96I 170 GGACC G^G[ACGT]CC ScaI 133 AGTACT AGT^ACT ScrFI 331 CCAGG CC^[ACGT]GG TaqI 456 TCGA T^CGA Tsp509I 428 AATT ^AATT TthHB8I 456 TCGA T^CGA XcmI 401 CCAATAAATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 45 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AfaI 134 GTAC GT^AC 179 GTAC BsrDI 220 CATTGC GCAATG(2/0) 367 GCAATG Cac8I 262 GCCGGC GC[ACGT]^[ACGT]GC 276 GCTCGC HgaI 271 GCGTC GACGC(5/10) 444 GCGTC MseI 79 TTAA T^TAA 116 TTAA NlaIV 169 GGGACC GG[ACGT]^[ACGT]CC 326 GGTGCC RsaI 134 GTAC GT^AC 179 GTAC Tru9I 79 TTAA T^TAA 116 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### BbvI DdeI Fnu4HI ItaI MaeIII MboII MwoI TseI Tsp45I