################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BbrPI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI BstEII BstPI Bsu36I CelII ClaI CpoI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MluNI MroI MscI MslI MunI NdeI NotI NruI NsiI PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI SexAI SfiI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XcmI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### Asp700I 997 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 2549 GGATCC G^GATCC BbsI 1190 GAAGAC GAAGAC(2/6) BcgI 270 CGAGGCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2067 GTATCC GTATCC(6/5) BclI 1380 TGATCA T^GATCA BpuAI 1190 GAAGAC GAAGAC(2/6) BsaWI 143 ACCGGA [AT]^CCGG[AT] BsmBI 19 GAGACG CGTCTC(1/5) BsmI 1370 GAATGC GAATGC(1/-1) BsrBI 165 GAGCGG CCGCTC(-3/-3) BssHII 714 GCGCGC G^CGCGC Bst1107I 1711 GTATAC GTA^TAC BstBI 2048 TTCGAA TT^CGAA BstXI 650 CCACCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1711 GTATAC GTA^TAC DraI 2056 TTTAAA TTT^AAA DraIII 849 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 21 GACGCTCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 912 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 1380 TGATCA T^GATCA MaeII 1836 ACGT A^CGT NheI 2682 GCTAGC G^CTAGC NspV 2048 TTCGAA TT^CGAA PaeR7I 25 CTCGAG C^TCGAG PflMI 882 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ScaI 1325 AGTACT AGT^ACT SfuI 2048 TTCGAA TT^CGAA SmlI 25 CTCGAG C^T[CT][GA]AG TfiI 2833 GAATC G^A[AT]TC Tsp45I 2162 GTGAC ^GT[GC]AC Van91I 882 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 25 CTCGAG C^TCGAG XmnI 997 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 82 AAATTC [GA]^AATT[CT] 1390 AAATTT Alw44I 721 GTGCAC G^TGCAC 2609 GTGCAC ApaI 238 GGGCCC GGGCC^C 307 GGGCCC ApaLI 721 GTGCAC G^TGCAC 2609 GTGCAC ApoI 82 AAATTC [GA]^AATT[CT] 1390 AAATTT BbvCI 966 CCTCAGC CCTCAGC(-5/-2) 2573 CCTCAGC BglI 248 GCCCGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 502 GCCCGGCCGGC BglII 1311 AGATCT A^GATCT 2517 AGATCT BsaI 934 GAGACC GGTCTC(1/5) 564 GGTCTC BsgI 678 GTGCAG GTGCAG(16/14) 2129 GTGCAG BsiEI 505 CGGCCG CG[GA][CT]^CG 668 CGGCCG EaeI 505 CGGCCG [CT]^GGCC[GA] 668 CGGCCG EagI 505 CGGCCG C^GGCCG 668 CGGCCG EclXI 505 CGGCCG C^GGCCG 668 CGGCCG Eco52I 505 CGGCCG C^GGCCG 668 CGGCCG EcoT14I 1211 CCATGG C^C[AT][AT]GG 2669 CCATGG KspI 234 CCGCGG CCGC^GG 376 CCGCGG NaeI 208 GCCGGC GCC^GGC 507 GCCGGC NcoI 1211 CCATGG C^CATGG 2669 CCATGG NgoMIV 208 GCCGGC G^CCGGC 507 GCCGGC NspI 794 GCATGT [GA]CATG^[CT] 1509 ACATGC PstI 494 CTGCAG CTGCA^G 1795 CTGCAG SacII 234 CCGCGG CCGC^GG 376 CCGCGG SapI 1789 GAAGAGC GCTCTTC(1/4) 924 GCTCTTC StyI 1211 CCATGG C^C[AT][AT]GG 2669 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PleI PpuMI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI TaqI Tru9I TseI Tsp509I TspRI TthHB8I XhoII XmaCI XmaI