Restriction map for
pFN21AB0466
(Circular) (Six-base) MAPSORT of: pFN21AB0466.seq Check: 2184 from: 1 to: 7669
pFN21AB0466 7669 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2909 2909
Size: 7669
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5479 278
Size: 53 83 186 936 3943 2468
Fragments arranged by size:
3943 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2877 3256 3717 5012 1937
Size: 940 379 461 1295 4594
Fragments arranged by size:
4594 1295 940 461 379
Acc65I G'GTAC_C
Cuts at: 4990 4990
Size: 7669
AclI AA'CG_TT
Cuts at: 1563 4968 5796 6169 1563
Size: 3405 828 373 3063
Fragments arranged by size:
3405 3063 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2490 2993 3593 3794 4227 4662 5732 7116
Size: 858 503 600 201 433 435 1070 1384
Cuts at: 7116 7384 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1070 858 600 503 435 433
268 201
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7472
AflIII A'CryG_T
Cuts at: 1203 6568 7545 1203
Size: 5365 977 1327
Fragments arranged by size:
5365 1327 977
AgeI A'CCGG_T
Cuts at: 6500 6500
Size: 7669
AhdI GACnn_n'nnGTC
Cuts at: 6398 6398
Size: 7669
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7669
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7669
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4729 4761 4729
Size: 32 7637
AlwNI CAG_nnn'CTG
Cuts at: 2498 2716 2737 3422 3776 5412 6984 2498
Size: 218 21 685 354 1636 1572 3183
Fragments arranged by size:
3183 1636 1572 685 354 218 21
ApaI G_GGCC'C
Cuts at: 2247 2316 2247
Size: 69 7600
ApaLI G'TGCA_C
Cuts at: 2726 4614 5726 6882 2726
Size: 1888 1112 1156 3513
Fragments arranged by size:
3513 1888 1156 1112
ApoI r'AATT_y
Cuts at: 1493 2087 3395 4978 5204 5356 7465 1493
Size: 594 1308 1583 226 152 2109 1697
Fragments arranged by size:
2109 1697 1583 1308 594 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6223 160
Size: 872 5191 1606
Fragments arranged by size:
5191 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7669
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 2178 2273 2309 2452 2783 2828
Size: 73 87 148 95 36 143 331 45
Cuts at: 2828 2951 4994 1870
Size: 123 2043 4545
Fragments arranged by size:
4545 2043 331 148 143 123 95 87
73 45 36
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4055 4088 4055
Size: 33 7636
BamHI G'GATC_C
Cuts at: 4554 4999 5366 4554
Size: 445 367 6857
Fragments arranged by size:
6857 445 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2204 2515 2614 2822 4990
Size: 325 217 627 417 311 99 208 2168
Cuts at: 4990 6445 618
Size: 1455 1842
Fragments arranged by size:
2168 1842 1455 627 417 325 311 217
208 99
BanII G_rGCy'C
Cuts at: 1391 2247 2309 2316 2327 2432 3039 4670 4988
Size: 856 62 7 11 105 607 1631 318
Cuts at: 4988 1391
Size: 4072
Fragments arranged by size:
4072 1631 856 607 318 105 62 11
7
BbeI G_GCGC'C
Cuts at: 2208 2519 2826 2208
Size: 311 307 7051
Fragments arranged by size:
7051 311 307
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3202 928
Size: 971 1303 5395
Fragments arranged by size:
5395 1303 971
BbvCI CC'TCA_GC
Cuts at: 2972 4579 2972
Size: 1607 6062
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2264 2298 5860 5894 1903
Size: 34 327 34 3562 34 3678
Fragments arranged by size:
3678 3562 327 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4083 5561 6771 4083
Size: 1478 1210 4981
Fragments arranged by size:
4981 1478 1210
BclI T'GATC_A
Cuts at: 1548 3385 1548
Size: 1837 5832
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2259 2513 6280
Size: 107 122 71 631 605 587 254 3767
Cuts at: 6280 136
Size: 1525
Fragments arranged by size:
3767 1525 631 605 587 254 122 107
71
BglII A'GATC_T
Cuts at: 3316 4522 7664 3316
Size: 1206 3142 3321
Fragments arranged by size:
3321 3142 1206
BlpI GC'TnA_GC
Cuts at: 5086 5086
Size: 7669
Bme1580I G_kGCm'C
Cuts at: 1790 2247 2316 2730 4576 4618 5730 6886 1790
Size: 457 69 414 1846 42 1112 1156 2573
Fragments arranged by size:
2573 1846 1156 1112 457 414 69 42
BmgBI CAC'GTC
Cuts at: 1318 3842 1318
Size: 2524 5145
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2050 2426 4969
Size: 416 53 83 399 24 633 376 2543
Cuts at: 4969 5400 6358 442
Size: 431 958 1753
Fragments arranged by size:
2543 1753 958 633 431 416 399 376
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4691 1056
Size: 3635 4034
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2362 2362
Size: 7669
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2039 2684 2855 3428 3773 4535 6329 1239
Size: 800 645 171 573 345 762 1794 2579
Fragments arranged by size:
2579 1794 800 762 645 573 345 171
Bpu10I CC'TnA_GC
Cuts at: 2972 4491 4579 2972
Size: 1519 88 6062
Fragments arranged by size:
6062 1519 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5794 6659 6957 5794
Size: 865 298 6506
Fragments arranged by size:
6506 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2575 2933 6332 882
Size: 617 1076 358 3399 2219
Fragments arranged by size:
3399 2219 1076 617 358
BsaAI yAC'GTr
Cuts at: 493 1204 5460 493
Size: 711 4256 2702
Fragments arranged by size:
4256 2702 711
BsaBI GATnn'nnATC
Cuts at: 7663 7663
Size: 7669
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2205 2516 2823 5476
Size: 53 83 186 936 672 311 307 2653
Cuts at: 5476 5858 6493 275
Size: 382 635 1451
Fragments arranged by size:
2653 1451 936 672 635 382 311 307
186 83 53
BsaWI w'CCGG_w
Cuts at: 2148 6102 6500 6774 6921 7332 2148
Size: 3954 398 274 147 411 2485
Fragments arranged by size:
3954 2485 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4729 4759 1178
Size: 30 165 30 3326 30 4088
Fragments arranged by size:
4088 3326 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 2182 2340 2591 2969 4443 4669 4672
Size: 850 146 158 251 378 1474 226 3
Cuts at: 4672 4952 1186
Size: 280 3903
Fragments arranged by size:
3903 1474 850 378 280 251 226 158
146 3
BseYI C'CCAG_C
Cuts at: 2688 6872 7368 2688
Size: 4184 496 2989
Fragments arranged by size:
4184 2989 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2704 4155 1163
Size: 573 968 1451 4677
Fragments arranged by size:
4677 1451 968 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2513 2676 5119 5880 6908 1540
Size: 275 186 512 163 2443 761 1028 2301
Fragments arranged by size:
2443 2301 1028 761 512 275 186 163
BsiHKAI G_wGCw'C
Cuts at: 2327 2360 2730 2744 2931 3039 4618 4670 5730
Size: 33 370 14 187 108 1579 52 1060
Cuts at: 5730 5815 6886 2327
Size: 85 1071 3110
Fragments arranged by size:
3110 1579 1071 1060 370 187 108 85
52 33 14
BsmI GAATG_Cn'
Cuts at: 3381 5180 5273 3381
Size: 1799 93 5777
Fragments arranged by size:
5777 1799 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2018 2018
Size: 7669
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2247 2309 2316 2327 2360 2432 2730
Size: 399 457 62 7 11 33 72 298
Cuts at: 2730 2744 2931 3039 4576 4618 4670 4988 5730
Size: 14 187 108 1537 42 52 318 742
Cuts at: 5730 5815 6886 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1537 1071 742 457 399 318 298
187 108 85 72 62 52 42 33
14 11 7
BspEI T'CCGG_A
Cuts at: 7332 7332
Size: 7669
BspHI T'CATG_A
Cuts at: 5558 7288 5558
Size: 1730 5939
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2147 2724 2909 5024 844
Size: 1303 577 185 2115 3489
Fragments arranged by size:
3489 2115 1303 577 185
BsrBI CCG'CTC
Cuts at: 1394 1823 2172 5556 1394
Size: 429 349 3384 3507
Fragments arranged by size:
3507 3384 429 349
BsrDI GCAATG_nn'
Cuts at: 65 1248 6164 6338 65
Size: 1183 4916 174 1396
Fragments arranged by size:
4916 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2213 2512 2773 6313 6500 1700
Size: 110 403 299 261 3540 187 2869
Fragments arranged by size:
3540 2869 403 299 261 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7669
BssHII G'CGCG_C
Cuts at: 1925 2719 1925
Size: 794 6875
BssSI C'ACGA_G
Cuts at: 5729 6741 5729
Size: 1012 6657
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2498 3422 3541 3776 794
Size: 1704 924 119 235 4687
Fragments arranged by size:
4687 1704 924 235 119
BstBI TT'CG_AA
Cuts at: 4054 6473 4054
Size: 2419 5250
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7669
BstXI CCAn_nnnn'nTGG
Cuts at: 2662 2662
Size: 7669
BstYI r'GATC_y
Cuts at: 1972 2913 3316 4522 4554 4999 5366 5751 5768
Size: 941 403 1206 32 445 367 385 17
Cuts at: 5768 7220 7306 7318 7664 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1206 941 445 403 385 367
346 86 32 17 12
BstZ17I GTA'TAC
Cuts at: 3718 3718
Size: 7669
BtgI C'CryG_G
Cuts at: 513 1065 2239 2381 2887 3216 4674 513
Size: 552 1174 142 506 329 1458 3508
Fragments arranged by size:
3508 1458 1174 552 506 329 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6930
Fragments arranged by size:
6930 683 56
BtsI GCAGTG_nn'
Cuts at: 2280 5185 5979 5999 2280
Size: 2905 794 20 3950
Fragments arranged by size:
3950 2905 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7669
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7634
DraI TTT'AAA
Cuts at: 4063 4973 4063
Size: 910 6759
DraIII CAC_nnn'GTG
Cuts at: 2859 2859
Size: 7669
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2032 6676 809
Size: 507 716 4644 1802
Fragments arranged by size:
4644 1802 716 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2510 2673 5116
Size: 54 1425 50 275 18 680 163 2443
Cuts at: 5116 6005 7634 8
Size: 889 1629 43
Fragments arranged by size:
2443 1629 1425 889 680 275 163 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2510 2673 5116 1537
Size: 275 698 163 2443 4090
Fragments arranged by size:
4090 2443 698 275 163
EarI CTCTTCn'nnn_
Cuts at: 1353 2673 2936 3522 3642 3756 3789 4191 4203
Size: 1320 263 586 120 114 33 402 12
Cuts at: 4203 4219 4365 4428 4544 4929 5599 1353
Size: 16 146 63 116 385 670 3423
Fragments arranged by size:
3423 1320 670 586 402 385 263 146
120 116 114 63 33 16 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2232 2335 2351 2426 6256 6630
Size: 1064 138 643 103 16 75 3830 374
Cuts at: 6630 6776 387
Size: 146 1280
Fragments arranged by size:
3830 1280 1064 643 374 146 138 103
75 16
EcoICRI GAG'CTC
Cuts at: 2325 3037 4668 2325
Size: 712 1631 5326
Fragments arranged by size:
5326 1631 712
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2490 2684 2855 2993 3428 3593
Size: 393 407 451 194 171 138 435 165
Cuts at: 3593 3773 3794 4227 4535 4662 5732 6329 7116
Size: 180 21 433 308 127 1070 597 787
Cuts at: 7116 7384 1239
Size: 268 1524
Fragments arranged by size:
1524 1070 787 597 451 435 433 407
393 308 268 194 180 171 165 138
127 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2921 2921
Size: 7669
EcoO109I rG'GnC_Cy
Cuts at: 2313 2377 2713 3236 2313
Size: 64 336 523 6746
Fragments arranged by size:
6746 523 336 64
EcoRI G'AATT_C
Cuts at: 4978 5356 4978
Size: 378 7291
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3023 4106 3023
Size: 1083 6586
FspI TGC'GCA
Cuts at: 6175 6175
Size: 7669
HaeII r_GCGC'y
Cuts at: 1678 2208 2519 2559 2627 2826 6816 1678
Size: 530 311 40 68 199 3990 2531
Fragments arranged by size:
3990 2531 530 311 199 68 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2651 2683 2932 2964 3189
Size: 32 135 32 1079 32 249 32 225
Cuts at: 3189 3221 6310 6342 6384 6416 1373
Size: 32 3089 32 42 32 2626
Fragments arranged by size:
3089 2626 1079 249 225 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5013 5259 669
Size: 988 281 3075 246 3079
Fragments arranged by size:
3079 3075 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7669
HpaI GTT'AAC
Cuts at: 5259 5259
Size: 7669
KasI G'GCGC_C
Cuts at: 2204 2515 2822 2204
Size: 311 307 7051
Fragments arranged by size:
7051 311 307
KpnI G_GTAC'C
Cuts at: 4994 4994
Size: 7669
MfeI C'AATT_G
Cuts at: 5268 5268
Size: 7669
MluI A'CGCG_T
Cuts at: 7545 7545
Size: 7669
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2016 2253 2739 3075 3943 4454 6783 6967
Size: 711 237 486 336 868 511 2329 184
Cuts at: 6967 1305
Size: 2007
Fragments arranged by size:
2329 2007 868 711 511 486 336 237
184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5847
Fragments arranged by size:
5847 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5627 5986 6145 518
Size: 648 4461 359 159 2042
Fragments arranged by size:
4461 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2241 2383 2498 2688 2737 3013 3283 3419
Size: 635 142 115 190 49 276 270 136
Cuts at: 3419 3508 3829 4606 5085 5762 6910 7155 1606
Size: 89 321 777 479 677 1148 245 2120
Fragments arranged by size:
2120 1148 777 677 635 479 321 276
270 245 190 142 136 115 89 49
NaeI GCC'GGC
Cuts at: 2215 2514 2215
Size: 299 7370
NarI GG'CG_CC
Cuts at: 2205 2516 2823 2205
Size: 311 307 7051
Fragments arranged by size:
7051 311 307
NcoI C'CATG_G
Cuts at: 513 1065 3216 4674 513
Size: 552 2151 1458 3508
Fragments arranged by size:
3508 2151 1458 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7669
NgoMIV G'CCGG_C
Cuts at: 2213 2512 2213
Size: 299 7370
NheI G'CTAG_C
Cuts at: 1052 4687 1052
Size: 3635 4034
NotI GC'GGCC_GC
Cuts at: 5116 5116
Size: 7669
NspI r_CATG'y
Cuts at: 1132 1229 2803 3518 5027 6572 1132
Size: 97 1574 715 1509 1545 2229
Fragments arranged by size:
2229 1574 1545 1509 715 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7669
PciI A'CATG_T
Cuts at: 6568 6568
Size: 7669
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2893 1108
Size: 1785 5884
PfoI T'CCnGG_A
Cuts at: 2709 3030 2709
Size: 321 7348
PmeI GTTT'AAAC
Cuts at: 4973 4973
Size: 7669
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4729 4761 5738 5770 7251 7283 4729
Size: 32 977 32 1481 32 5115
Fragments arranged by size:
5115 1481 977 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2377 2713 3236 2377
Size: 336 523 6810
Fragments arranged by size:
6810 523 336
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7669
PsiI TTA'TAA
Cuts at: 5239 5239
Size: 7669
PspOMI G'GGCC_C
Cuts at: 2243 2312 2243
Size: 69 7600
PspXI vC'TCGA_Gb
Cuts at: 2030 2030
Size: 7669
PstI C_TGCA'G
Cuts at: 830 2503 3804 5021 830
Size: 1673 1301 1217 3478
Fragments arranged by size:
3478 1673 1301 1217
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7669
PvuII CAG'CTG
Cuts at: 2688 2737 3829 4606 2688
Size: 49 1092 777 5751
Fragments arranged by size:
5751 1092 777 49
SacI G_AGCT'C
Cuts at: 2327 3039 4670 2327
Size: 712 1631 5326
Fragments arranged by size:
5326 1631 712
SacII CC_GC'GG
Cuts at: 2242 2384 2242
Size: 142 7527
SalI G'TCGA_C
Cuts at: 1936 5011 1936
Size: 3075 4594
SanDI GG'GwC_CC
Cuts at: 2377 2377
Size: 7669
SapI GCTCTTCn'nnn_
Cuts at: 2936 3789 2936
Size: 853 6816
SbfI CC_TGCA'GG
Cuts at: 5021 5021
Size: 7669
ScaI AGT'ACT
Cuts at: 3332 5917 7629 3332
Size: 2585 1712 3372
Fragments arranged by size:
3372 2585 1712
SfcI C'TryA_G
Cuts at: 826 1045 2499 3109 3155 3800 5017 6152 6833
Size: 219 1454 610 46 645 1217 1135 681
Cuts at: 6833 7024 826
Size: 191 1471
Fragments arranged by size:
1471 1454 1217 1135 681 645 610 219
191 46
SfoI GGC'GCC
Cuts at: 2206 2517 2824 2206
Size: 311 307 7051
Fragments arranged by size:
7051 311 307
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7669
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7669
SmaI CCC'GGG
Cuts at: 1872 2180 2311 2454 4996 1872
Size: 308 131 143 2542 4545
Fragments arranged by size:
4545 2542 308 143 131
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2030 5773 6674 6936 820
Size: 197 926 87 3743 901 262 1553
Fragments arranged by size:
3743 1553 926 901 262 197 87
SnaBI TAC'GTA
Cuts at: 493 5460 493
Size: 4967 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7669
SphI G_CATG'C
Cuts at: 1132 5027 1132
Size: 3895 3774
SspI AAT'ATT
Cuts at: 5 52 5593 5
Size: 47 5541 2081
Fragments arranged by size:
5541 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7669
StyI C'CwwG_G
Cuts at: 513 1065 3216 4674 5108 513
Size: 552 2151 1458 434 3074
Fragments arranged by size:
3074 2151 1458 552 434
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2032 2852 2957 4687 5696 5713 5866
Size: 1255 452 820 105 1730 1009 17 153
Cuts at: 5866 6051 6496 325
Size: 185 445 1498
Fragments arranged by size:
1730 1498 1255 1009 820 452 445 185
153 105 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3301 3330 3622
Size: 274 80 33 51 1443 1324 29 292
Cuts at: 3622 3964 5915 7627 96
Size: 342 1951 1712 138
Fragments arranged by size:
1951 1712 1443 1324 342 292 274 138
80 51 33 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4866 4898 7629 7661 4866
Size: 32 2731 32 4874
Fragments arranged by size:
4874 2731 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7669
XbaI T'CTAG_A
Cuts at: 5005 5005
Size: 7669
XhoI C'TCGA_G
Cuts at: 1943 2030 1943
Size: 87 7582
XmaI C'CCGG_G
Cuts at: 1870 2178 2309 2452 4994 1870
Size: 308 131 143 2542 4545
Fragments arranged by size:
4545 2542 308 143 131
XmnI GAAnn'nnTTC
Cuts at: 3006 5798 3006
Size: 2792 4877
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5477 276
Size: 53 83 186 936 3943 2468
Fragments arranged by size:
3943 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BfrBI BsiWI Bsu36I EcoRV FseI
FspAI NruI NsiI PacI PmlI PsrI RsrII SexAI
SfiI SrfI SwaI XcmI