(Linear) (Six-base) MAPSORT of: KIBB0466.seq Check: 3927 from: 1 to: 2964 KIBB0466.orf With 182 enzymes: SgfI * July 15, 2010 18:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 904 2964 Size: 904 2060 AccI GT'mk_AC Cuts at: 0 872 1251 1712 2964 Size: 872 379 461 1252 Fragments arranged by size: 1252 872 461 379 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 485 988 1588 1789 2222 2657 2964 Size: 485 503 600 201 433 435 307 Fragments arranged by size: 600 503 485 435 433 307 201 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2724 2756 2964 Size: 2724 32 208 Fragments arranged by size: 2724 208 32 AlwNI CAG_nnn'CTG Cuts at: 0 493 711 732 1417 1771 2964 Size: 493 218 21 685 354 1193 Fragments arranged by size: 1193 685 493 354 218 21 ApaI G_GGCC'C Cuts at: 0 242 311 2964 Size: 242 69 2653 Fragments arranged by size: 2653 242 69 ApaLI G'TGCA_C Cuts at: 0 721 2609 2964 Size: 721 1888 355 Fragments arranged by size: 1888 721 355 ApoI r'AATT_y Cuts at: 0 82 1390 2964 Size: 82 1308 1574 Fragments arranged by size: 1574 1308 82 AvaI C'yCGr_G Cuts at: 0 25 173 268 304 447 778 823 946 Size: 25 148 95 36 143 331 45 123 Cuts at: 946 2964 Size: 2018 Fragments arranged by size: 2018 331 148 143 123 95 45 36 25 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2050 2083 2964 Size: 2050 33 881 Fragments arranged by size: 2050 881 33 BamHI G'GATC_C Cuts at: 0 2549 2964 Size: 2549 415 BanI G'GyrC_C Cuts at: 0 199 510 609 817 2964 Size: 199 311 99 208 2147 Fragments arranged by size: 2147 311 208 199 99 BanII G_rGCy'C Cuts at: 0 242 304 311 322 427 1034 2665 2964 Size: 242 62 7 11 105 607 1631 299 Fragments arranged by size: 1631 607 299 242 105 62 11 7 BbeI G_GCGC'C Cuts at: 0 203 514 821 2964 Size: 203 311 307 2143 Fragments arranged by size: 2143 311 307 203 BbsI GAAGACnn'nnnn_ Cuts at: 0 1197 2964 Size: 1197 1767 BbvCI CC'TCA_GC Cuts at: 0 967 2574 2964 Size: 967 1607 390 Fragments arranged by size: 1607 967 390 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 259 293 2964 Size: 259 34 2671 Fragments arranged by size: 2671 259 34 BciVI GTATCCnnnnn_n' Cuts at: 0 2078 2964 Size: 2078 886 BclI T'GATC_A Cuts at: 0 1380 2964 Size: 1380 1584 BglI GCCn_nnn'nGGC Cuts at: 0 254 508 2964 Size: 254 254 2456 Fragments arranged by size: 2456 254 254 BglII A'GATC_T Cuts at: 0 1311 2517 2964 Size: 1311 1206 447 Fragments arranged by size: 1311 1206 447 Bme1580I G_kGCm'C Cuts at: 0 242 311 725 2571 2613 2964 Size: 242 69 414 1846 42 351 Fragments arranged by size: 1846 414 351 242 69 42 BmgBI CAC'GTC Cuts at: 0 1837 2964 Size: 1837 1127 BmrI ACTGGGnnnn_n' Cuts at: 0 45 421 2964 Size: 45 376 2543 Fragments arranged by size: 2543 376 45 BmtI G_CTAG'C Cuts at: 0 2686 2964 Size: 2686 278 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 357 2964 Size: 357 2607 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 679 850 1423 1768 2530 2964 Size: 34 645 171 573 345 762 434 Fragments arranged by size: 762 645 573 434 345 171 34 Bpu10I CC'TnA_GC Cuts at: 0 967 2486 2574 2964 Size: 967 1519 88 390 Fragments arranged by size: 1519 967 390 88 BsaI GGTCTCn'nnnn_ Cuts at: 0 570 928 2964 Size: 570 358 2036 Fragments arranged by size: 2036 570 358 BsaHI Gr'CG_yC Cuts at: 0 200 511 818 2964 Size: 200 311 307 2146 Fragments arranged by size: 2146 311 307 200 BsaWI w'CCGG_w Cuts at: 0 143 2964 Size: 143 2821 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2724 2754 2964 Size: 2724 30 210 Fragments arranged by size: 2724 210 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 31 177 335 586 964 2438 2664 2667 Size: 31 146 158 251 378 1474 226 3 Cuts at: 2667 2947 2964 Size: 280 17 Fragments arranged by size: 1474 378 280 251 226 158 146 31 17 3 BseYI C'CCAG_C Cuts at: 0 683 2964 Size: 683 2281 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 699 2150 2964 Size: 699 1451 814 Fragments arranged by size: 1451 814 699 BsiEI CG_ry'CG Cuts at: 0 508 671 2964 Size: 508 163 2293 Fragments arranged by size: 2293 508 163 BsiHKAI G_wGCw'C Cuts at: 0 322 355 725 739 926 1034 2613 2665 Size: 322 33 370 14 187 108 1579 52 Cuts at: 2665 2964 Size: 299 Fragments arranged by size: 1579 370 322 299 187 108 52 33 14 BsmI GAATG_Cn' Cuts at: 0 1376 2964 Size: 1376 1588 BsmBI CGTCTCn'nnnn_ Cuts at: 0 13 2964 Size: 13 2951 Bsp1286I G_dGCh'C Cuts at: 0 242 304 311 322 355 427 725 739 Size: 242 62 7 11 33 72 298 14 Cuts at: 739 926 1034 2571 2613 2665 2964 Size: 187 108 1537 42 52 299 Fragments arranged by size: 1537 299 298 242 187 108 72 62 52 42 33 14 11 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 142 719 904 2964 Size: 142 577 185 2060 Fragments arranged by size: 2060 577 185 142 BsrBI CCG'CTC Cuts at: 0 167 2964 Size: 167 2797 BsrFI r'CCGG_y Cuts at: 0 208 507 768 2964 Size: 208 299 261 2196 Fragments arranged by size: 2196 299 261 208 BssHII G'CGCG_C Cuts at: 0 714 2964 Size: 714 2250 BstAPI GCAn_nnn'nTGC Cuts at: 0 493 1417 1536 1771 2964 Size: 493 924 119 235 1193 Fragments arranged by size: 1193 924 493 235 119 BstBI TT'CG_AA Cuts at: 0 2049 2964 Size: 2049 915 BstXI CCAn_nnnn'nTGG Cuts at: 0 657 2964 Size: 657 2307 BstYI r'GATC_y Cuts at: 0 908 1311 2517 2549 2964 Size: 908 403 1206 32 415 Fragments arranged by size: 1206 908 415 403 32 BstZ17I GTA'TAC Cuts at: 0 1713 2964 Size: 1713 1251 BtgI C'CryG_G Cuts at: 0 234 376 882 1211 2669 2964 Size: 234 142 506 329 1458 295 Fragments arranged by size: 1458 506 329 295 234 142 BtsI GCAGTG_nn' Cuts at: 0 275 2964 Size: 275 2689 DraI TTT'AAA Cuts at: 0 2058 2964 Size: 2058 906 DraIII CAC_nnn'GTG Cuts at: 0 854 2964 Size: 854 2110 DrdI GACnn_nn'nnGTC Cuts at: 0 27 2964 Size: 27 2937 EaeI y'GGCC_r Cuts at: 0 505 668 2964 Size: 505 163 2296 Fragments arranged by size: 2296 505 163 EagI C'GGCC_G Cuts at: 0 505 668 2964 Size: 505 163 2296 Fragments arranged by size: 2296 505 163 EarI CTCTTCn'nnn_ Cuts at: 0 668 931 1517 1637 1751 1784 2186 2198 Size: 668 263 586 120 114 33 402 12 Cuts at: 2198 2214 2360 2423 2539 2924 2964 Size: 16 146 63 116 385 40 Fragments arranged by size: 668 586 402 385 263 146 120 116 114 63 40 33 16 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 227 330 346 421 2964 Size: 227 103 16 75 2543 Fragments arranged by size: 2543 227 103 75 16 EcoICRI GAG'CTC Cuts at: 0 320 1032 2663 2964 Size: 320 712 1631 301 Fragments arranged by size: 1631 712 320 301 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 485 679 850 988 1423 1588 1768 Size: 34 451 194 171 138 435 165 180 Cuts at: 1768 1789 2222 2530 2657 2964 Size: 21 433 308 127 307 Fragments arranged by size: 451 435 433 308 307 194 180 171 165 138 127 34 21 EcoNI CCTnn'n_nnAGG Cuts at: 0 916 2964 Size: 916 2048 EcoO109I rG'GnC_Cy Cuts at: 0 308 372 708 1231 2964 Size: 308 64 336 523 1733 Fragments arranged by size: 1733 523 336 308 64 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1018 2101 2964 Size: 1018 1083 863 Fragments arranged by size: 1083 1018 863 HaeII r_GCGC'y Cuts at: 0 203 514 554 622 821 2964 Size: 203 311 40 68 199 2143 Fragments arranged by size: 2143 311 203 199 68 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 646 678 927 959 1184 1216 2964 Size: 646 32 249 32 225 32 1748 Fragments arranged by size: 1748 646 249 225 32 32 32 KasI G'GCGC_C Cuts at: 0 199 510 817 2964 Size: 199 311 307 2147 Fragments arranged by size: 2147 311 307 199 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 11 248 734 1070 1938 2449 2964 Size: 11 237 486 336 868 511 515 Fragments arranged by size: 868 515 511 486 336 237 11 MspA1I CmG'CkG Cuts at: 0 236 378 493 683 732 1008 1278 1414 Size: 236 142 115 190 49 276 270 136 Cuts at: 1414 1503 1824 2601 2964 Size: 89 321 777 363 Fragments arranged by size: 777 363 321 276 270 236 190 142 136 115 89 49 NaeI GCC'GGC Cuts at: 0 210 509 2964 Size: 210 299 2455 Fragments arranged by size: 2455 299 210 NarI GG'CG_CC Cuts at: 0 200 511 818 2964 Size: 200 311 307 2146 Fragments arranged by size: 2146 311 307 200 NcoI C'CATG_G Cuts at: 0 1211 2669 2964 Size: 1211 1458 295 Fragments arranged by size: 1458 1211 295 NgoMIV G'CCGG_C Cuts at: 0 208 507 2964 Size: 208 299 2457 Fragments arranged by size: 2457 299 208 NheI G'CTAG_C Cuts at: 0 2682 2964 Size: 2682 282 NspI r_CATG'y Cuts at: 0 798 1513 2964 Size: 798 715 1451 Fragments arranged by size: 1451 798 715 PflMI CCAn_nnn'nTGG Cuts at: 0 888 2964 Size: 888 2076 PfoI T'CCnGG_A Cuts at: 0 704 1025 2964 Size: 704 321 1939 Fragments arranged by size: 1939 704 321 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2724 2756 2964 Size: 2724 32 208 Fragments arranged by size: 2724 208 32 PpuMI rG'GwC_Cy Cuts at: 0 372 708 1231 2964 Size: 372 336 523 1733 Fragments arranged by size: 1733 523 372 336 PspOMI G'GGCC_C Cuts at: 0 238 307 2964 Size: 238 69 2657 Fragments arranged by size: 2657 238 69 PspXI vC'TCGA_Gb Cuts at: 0 25 2964 Size: 25 2939 PstI C_TGCA'G Cuts at: 0 498 1799 2964 Size: 498 1301 1165 Fragments arranged by size: 1301 1165 498 PvuII CAG'CTG Cuts at: 0 683 732 1824 2601 2964 Size: 683 49 1092 777 363 Fragments arranged by size: 1092 777 683 363 49 SacI G_AGCT'C Cuts at: 0 322 1034 2665 2964 Size: 322 712 1631 299 Fragments arranged by size: 1631 712 322 299 SacII CC_GC'GG Cuts at: 0 237 379 2964 Size: 237 142 2585 Fragments arranged by size: 2585 237 142 SanDI GG'GwC_CC Cuts at: 0 372 2964 Size: 372 2592 SapI GCTCTTCn'nnn_ Cuts at: 0 931 1784 2964 Size: 931 853 1180 Fragments arranged by size: 1180 931 853 ScaI AGT'ACT Cuts at: 0 1327 2964 Size: 1327 1637 SfcI C'TryA_G Cuts at: 0 494 1104 1150 1795 2964 Size: 494 610 46 645 1169 Fragments arranged by size: 1169 645 610 494 46 SfoI GGC'GCC Cuts at: 0 201 512 819 2964 Size: 201 311 307 2145 Fragments arranged by size: 2145 311 307 201 SmaI CCC'GGG Cuts at: 0 175 306 449 2964 Size: 175 131 143 2515 Fragments arranged by size: 2515 175 143 131 SmlI C'TyrA_G Cuts at: 0 25 2964 Size: 25 2939 StyI C'CwwG_G Cuts at: 0 1211 2669 2964 Size: 1211 1458 295 Fragments arranged by size: 1458 1211 295 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 27 847 952 2682 2964 Size: 27 820 105 1730 282 Fragments arranged by size: 1730 820 282 105 27 TatI w'GTAC_w Cuts at: 0 1296 1325 1617 1959 2964 Size: 1296 29 292 342 1005 Fragments arranged by size: 1296 1005 342 292 29 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2861 2893 2964 Size: 2861 32 71 Fragments arranged by size: 2861 71 32 XhoI C'TCGA_G Cuts at: 0 25 2964 Size: 25 2939 XmaI C'CCGG_G Cuts at: 0 173 304 447 2964 Size: 173 131 143 2517 Fragments arranged by size: 2517 173 143 131 XmnI GAAnn'nnTTC Cuts at: 0 1001 2964 Size: 1001 1963 Enzymes that do cut: AarI AccI AcuI AloI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstBI BstXI BstYI BstZ17I BtgI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI MmeI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PflMI PfoI PpiI PpuMI PspOMI PspXI PstI PvuII SacI SacII SanDI SapI ScaI SfcI SfoI SmaI SmlI StyI TaqII TatI TstI XhoI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AscI AseI AsiSI AvrII BfrBI BlpI BpuEI BsaAI BsaBI BsiWI BspEI BspHI BsrDI BsrGI BssSI BstEII Bsu36I BtgZI ClaI CspCI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI MslI NdeI NotI NruI NsiI PacI PasI PciI PmeI PmlI PshAI PsiI PsrI PvuI RsrII SalI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI ZraI