Restriction map for
pFN21AE1881
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AgeI AhdI Alw44I
AlwI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BseAI BsiEI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspEI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstYI BstZ17I CelII
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoT22I FbaI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MflI MluI MluNI MroI MscI
MvnI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI PstI PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AciI 890 GCGG CCGC(-3/-1)
AclI 44 AACGTT AA^CGTT
AfaI 882 GTAC GT^AC
AflIII 486 ACACGT A^C[GA][CT]GT
AlwNI 1174 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1073 CCTCAGG CC^T[ACGT]AGG
Asp700I 160 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 124 GGACC G^G[AT]CC
BbsI 238 GAAGAC GAAGAC(2/6)
BcnI 592 CCCGG CC^[GC]GG
BfaI 40 CTAG C^TAG
BglI 853 GCCAGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 238 GAAGAC GAAGAC(2/6)
BsaAI 112 TACGTA [CT]AC^GT[GA]
BsaWI 529 TCCGGT [AT]^CCGG[AT]
BsgI 602 GTGCAG GTGCAG(16/14)
BspDI 784 ATCGAT AT^CGAT
BspHI 689 TCATGA T^CATGA
BsrFI 1079 GCCGGC [GA]^CCGG[CT]
BstAPI 579 GCATGGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1108 CCAGATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1073 CCTCAGG CC^T[ACGT]AGG
CfoI 609 GCGC GCG^C
Cfr10I 1079 GCCGGC [GA]^CCGG[CT]
ClaI 784 ATCGAT AT^CGAT
DpnII 787 GATC ^GATC
DraII 123 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 1081 CGGCCA [CT]^GGCC[GA]
EarI 102 GAAGAG CTCTTC(1/4)
Eco81I 1073 CCTCAGG CC^T[ACGT]AGG
EcoO109I 123 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 796 GAATTC G^AATTC
EcoRV 279 GATATC GAT^ATC
EcoT14I 743 CCTTGG C^C[AT][AT]GG
FauI 890 GCGGG CCCGC(4/6)
FseI 1078 GGCCGGCC GGCCGG^CC
HhaI 609 GCGC GCG^C
HinP1I 609 GCGC G^CGC
Ksp632I 102 GAAGAG CTCTTC(1/4)
MaeI 40 CTAG C^TAG
MaeIII 1123 GTGAC ^GT[ACGT]AC
MboI 787 GATC ^GATC
MfeI 926 CAATTG C^AATTG
MslI 636 CATTGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 926 CAATTG C^AATTG
NaeI 1079 GCCGGC GCC^GGC
NciI 592 CCCGG CC^[GC]GG
NdeII 787 GATC ^GATC
NgoMIV 1079 GCCGGC G^CCGGC
NspI 1007 ACATGC [GA]CATG^[CT]
PpuMI 123 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 44 AACGTT AA^CGTT
RcaI 689 TCATGA T^CATGA
RsaI 882 GTAC GT^AC
SacI 1186 GAGCTC GAGCT^C
Sau3AI 787 GATC ^GATC
SnaBI 112 TACGTA TAC^GTA
StuI 860 AGGCCT AGG^CCT
StyI 743 CCTTGG C^C[AT][AT]GG
Tsp45I 1123 GTGAC ^GT[GC]AC
XmnI 160 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AcsI 796 GAATTC [GA]^AATT[CT]
1204 AAATTC
ApoI 796 GAATTC [GA]^AATT[CT]
1204 AAATTC
AspHI 151 GAGCAC G[AT]GC[AT]^C
1186 GAGCTC
BanII 907 GAGCCC G[GA]GC[CT]^C
1186 GAGCTC
BseRI 359 GAGGAG GAGGAG(10/8)
934 GAGGAG
BsiHKAI 151 GAGCAC G[AT]GC[AT]^C
1186 GAGCTC
DraI 48 TTTAAA TTT^AAA
672 TTTAAA
MseI 49 TTAA T^TAA
673 TTAA
MspA1I 987 CAGCTG C[AC]G^C[GT]G
1177 CAGCTG
PleI 132 GACTC GAGTC(4/5)
366 GAGTC
PvuII 987 CAGCTG CAG^CTG
1177 CAGCTG
TaqI 180 TCGA T^CGA
785 TCGA
TfiI 779 GATTC G^A[AT]TC
1059 GAATC
Tru9I 49 TTAA T^TAA
673 TTAA
TthHB8I 180 TCGA T^CGA
785 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI BbvI BmyI BpmI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeII
MboII MnlI MspI MvaI MwoI
NlaIII NlaIV PflMI Sau96I ScrFI
SfaNI TseI Tsp509I TspRI Van91I