(Circular) (Six-base) MAPSORT of: pFN21AE1881.seq Check: 8462 from: 1 to: 5923 pFN21AE1881 5923 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3733 278 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3266 1937 Size: 1329 4594 Acc65I G'GTAC_C Cuts at: 3244 3244 Size: 5923 AclI AA'CG_TT Cuts at: 1563 2050 4050 4423 1563 Size: 487 2000 373 3063 Fragments arranged by size: 3063 2000 487 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2163 2430 2961 3986 5370 5638 1632 Size: 531 267 531 1025 1384 268 1917 Fragments arranged by size: 1917 1384 1025 531 531 268 267 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5726 AflIII A'CryG_T Cuts at: 1203 2491 4822 5799 1203 Size: 1288 2331 977 1327 Fragments arranged by size: 2331 1327 1288 977 AgeI A'CCGG_T Cuts at: 4754 4754 Size: 5923 AhdI GACnn_n'nnGTC Cuts at: 4652 4652 Size: 5923 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5923 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2575 1184 Size: 1391 4532 AlwNI CAG_nnn'CTG Cuts at: 3184 3666 5238 3184 Size: 482 1572 3869 Fragments arranged by size: 3869 1572 482 ApaLI G'TGCA_C Cuts at: 3980 5136 3980 Size: 1156 4767 ApoI r'AATT_y Cuts at: 1493 2801 3209 3232 3458 3610 5719 1493 Size: 1308 408 23 226 152 2109 1697 Fragments arranged by size: 2109 1697 1308 408 226 152 23 AseI AT'TA_AT Cuts at: 160 1032 4477 160 Size: 872 3445 1606 Fragments arranged by size: 3445 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 AvaI C'yCGr_G Cuts at: 1870 1943 3248 1870 Size: 73 1305 4545 Fragments arranged by size: 4545 1305 73 BamHI G'GATC_C Cuts at: 3253 3620 3253 Size: 367 5556 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3244 4699 618 Size: 325 217 627 1457 1455 1842 Fragments arranged by size: 1842 1457 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2916 3195 3242 1391 Size: 1525 279 47 4072 Fragments arranged by size: 4072 1525 279 47 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2250 928 Size: 971 351 4601 Fragments arranged by size: 4601 971 351 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4114 4148 1903 Size: 34 50 34 2093 34 3678 Fragments arranged by size: 3678 2093 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3815 5025 3815 Size: 1210 4713 BclI T'GATC_A Cuts at: 1548 1548 Size: 5923 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2864 4534 136 Size: 107 122 71 631 605 1192 1670 1525 Fragments arranged by size: 1670 1525 1192 631 605 122 107 71 BglII A'GATC_T Cuts at: 5918 5918 Size: 5923 BlpI GC'TnA_GC Cuts at: 3340 3340 Size: 5923 Bme1580I G_kGCm'C Cuts at: 1790 2595 3984 5140 1790 Size: 805 1389 1156 2573 Fragments arranged by size: 2573 1389 1156 805 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5923 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3067 3654 4612 Size: 416 53 83 399 24 1650 587 958 Cuts at: 4612 442 Size: 1753 Fragments arranged by size: 1753 1650 958 587 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5923 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2210 2210 Size: 5923 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2082 2123 2273 4583 1239 Size: 843 41 150 2310 2579 Fragments arranged by size: 2579 2310 843 150 41 Bpu10I CC'TnA_GC Cuts at: 2390 2390 Size: 5923 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4048 4913 5211 4048 Size: 865 298 4760 Fragments arranged by size: 4760 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4586 882 Size: 617 3087 2219 Fragments arranged by size: 3087 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2119 3714 493 Size: 711 915 1595 2702 Fragments arranged by size: 2702 1595 915 711 BsaBI GATnn'nnATC Cuts at: 5917 5917 Size: 5923 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3730 4112 4747 275 Size: 53 83 186 936 2197 382 635 1451 Fragments arranged by size: 2197 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2534 4356 4754 5028 5175 5586 2534 Size: 1822 398 274 147 411 2871 Fragments arranged by size: 2871 1822 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2055 2076 2085 2106 1178 Size: 30 165 30 652 21 9 21 4995 Fragments arranged by size: 4995 652 165 30 30 21 21 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2379 2954 1186 Size: 1193 575 4155 Fragments arranged by size: 4155 1193 575 BseYI C'CCAG_C Cuts at: 2994 3120 5126 5622 2994 Size: 126 2006 496 3295 Fragments arranged by size: 3295 2006 496 126 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2628 1163 Size: 573 892 4458 Fragments arranged by size: 4458 892 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3373 4134 5162 1540 Size: 275 186 1372 761 1028 2301 Fragments arranged by size: 2301 1372 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2160 3195 3984 4069 5140 2160 Size: 1035 789 85 1071 2943 Fragments arranged by size: 2943 1071 1035 789 85 BsmI GAATG_Cn' Cuts at: 3434 3527 3434 Size: 93 5830 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2160 2595 2916 3195 3242 3984 4069 Size: 399 370 435 321 279 47 742 85 Cuts at: 4069 5140 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 435 399 370 321 279 85 47 BspEI T'CCGG_A Cuts at: 5586 5586 Size: 5923 BspHI T'CATG_A Cuts at: 2694 3812 5542 2694 Size: 1118 1730 3075 Fragments arranged by size: 3075 1730 1118 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3278 844 Size: 2434 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3810 1394 Size: 429 1987 3507 Fragments arranged by size: 3507 1987 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4418 4592 65 Size: 1183 3170 174 1396 Fragments arranged by size: 3170 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3084 4567 4754 1700 Size: 110 1274 1483 187 2869 Fragments arranged by size: 2869 1483 1274 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5923 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5923 BssSI C'ACGA_G Cuts at: 3983 4995 3983 Size: 1012 4911 BstAPI GCAn_nnn'nTGC Cuts at: 794 2590 794 Size: 1796 4127 BstBI TT'CG_AA Cuts at: 4727 4727 Size: 5923 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5923 BstXI CCAn_nnnn'nTGG Cuts at: 3120 3120 Size: 5923 BstYI r'GATC_y Cuts at: 1972 3253 3620 4005 4022 5474 5560 5572 5918 Size: 1281 367 385 17 1452 86 12 346 Cuts at: 5918 1972 Size: 1977 Fragments arranged by size: 1977 1452 1281 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3079 3079 Size: 5923 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5371 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2305 487 Size: 683 56 1079 4105 Fragments arranged by size: 4105 1079 683 56 BtsI GCAGTG_nn' Cuts at: 2616 3439 4233 4253 2616 Size: 823 794 20 4286 Fragments arranged by size: 4286 823 794 20 ClaI AT'CG_AT Cuts at: 1554 2790 1554 Size: 1236 4687 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5888 DraI TTT'AAA Cuts at: 2055 2679 3227 2055 Size: 624 548 4751 Fragments arranged by size: 4751 624 548 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4930 809 Size: 507 3614 1802 Fragments arranged by size: 3614 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3086 3370 4259 Size: 54 1425 50 275 18 1256 284 889 Cuts at: 4259 5888 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1256 889 284 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3370 1537 Size: 275 1558 4090 Fragments arranged by size: 4090 1558 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2102 3853 1353 Size: 749 1751 3423 Fragments arranged by size: 3423 1751 749 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4510 4884 5030 387 Size: 1064 138 2921 374 146 1280 Fragments arranged by size: 2921 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3193 3193 Size: 5923 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2082 2123 2163 2273 2430 2961 3986 Size: 393 450 41 40 110 157 531 1025 Cuts at: 3986 4583 5370 5638 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1025 787 597 531 450 393 268 157 110 41 40 EcoO109I rG'GnC_Cy Cuts at: 2129 2129 Size: 5923 EcoRI G'AATT_C Cuts at: 2801 3232 3610 2801 Size: 431 378 5114 Fragments arranged by size: 5114 431 378 EcoRV GAT'ATC Cuts at: 2286 2286 Size: 5923 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2722 2722 Size: 5923 FseI GG_CCGG'CC Cuts at: 3088 3088 Size: 5923 FspI TGC'GCA Cuts at: 4429 4429 Size: 5923 HaeII r_GCGC'y Cuts at: 1678 5070 1678 Size: 3392 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2076 2108 2270 2302 2622 Size: 32 135 32 504 32 162 32 320 Cuts at: 2622 2654 4564 4596 4638 4670 1373 Size: 32 1910 32 42 32 2626 Fragments arranged by size: 2626 1910 504 320 162 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3267 3513 669 Size: 988 281 1329 246 3079 Fragments arranged by size: 3079 1329 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5923 HpaI GTT'AAC Cuts at: 3513 3513 Size: 5923 KpnI G_GTAC'C Cuts at: 3248 3248 Size: 5923 MfeI C'AATT_G Cuts at: 2931 3522 2931 Size: 591 5332 MluI A'CGCG_T Cuts at: 5799 5799 Size: 5923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5037 5221 1305 Size: 3732 184 2007 Fragments arranged by size: 3732 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4101 Fragments arranged by size: 4101 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2645 3881 4240 4399 518 Size: 648 1479 1236 359 159 2042 Fragments arranged by size: 2042 1479 1236 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2994 3184 3339 4016 5164 5409 1606 Size: 1388 190 155 677 1148 245 2120 Fragments arranged by size: 2120 1388 1148 677 245 190 155 NaeI GCC'GGC Cuts at: 3086 3086 Size: 5923 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5371 NdeI CA'TA_TG Cuts at: 387 387 Size: 5923 NgoMIV G'CCGG_C Cuts at: 3084 3084 Size: 5923 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5923 NotI GC'GGCC_GC Cuts at: 3370 3370 Size: 5923 NspI r_CATG'y Cuts at: 1132 1229 3016 3281 4826 1132 Size: 97 1787 265 1545 2229 Fragments arranged by size: 2229 1787 1545 265 97 PasI CC'CwG_GG Cuts at: 1348 3027 1348 Size: 1679 4244 PciI A'CATG_T Cuts at: 4822 4822 Size: 5923 PflMI CCAn_nnn'nTGG Cuts at: 1108 2015 2749 2838 3027 1108 Size: 907 734 89 189 4004 Fragments arranged by size: 4004 907 734 189 89 PmeI GTTT'AAAC Cuts at: 3227 3227 Size: 5923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3992 4024 5505 5537 3992 Size: 32 1481 32 4378 Fragments arranged by size: 4378 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2129 2129 Size: 5923 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5923 PsiI TTA'TAA Cuts at: 3493 3493 Size: 5923 PstI C_TGCA'G Cuts at: 830 3275 830 Size: 2445 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5923 PvuII CAG'CTG Cuts at: 2994 3184 2994 Size: 190 5733 SacI G_AGCT'C Cuts at: 3195 3195 Size: 5923 SalI G'TCGA_C Cuts at: 1936 3265 1936 Size: 1329 4594 SbfI CC_TGCA'GG Cuts at: 3275 3275 Size: 5923 ScaI AGT'ACT Cuts at: 4171 5883 4171 Size: 1712 4211 SfcI C'TryA_G Cuts at: 826 1045 3271 4406 5087 5278 826 Size: 219 2226 1135 681 191 1471 Fragments arranged by size: 2226 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5923 SmaI CCC'GGG Cuts at: 1872 3250 1872 Size: 1378 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4027 4928 5190 820 Size: 197 926 2084 901 262 1553 Fragments arranged by size: 2084 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2119 3714 493 Size: 1626 1595 2702 Fragments arranged by size: 2702 1626 1595 SpeI A'CTAG_T Cuts at: 152 152 Size: 5923 SphI G_CATG'C Cuts at: 1132 3281 1132 Size: 2149 3774 SspI AAT'ATT Cuts at: 5 52 3847 5 Size: 47 3795 2081 Fragments arranged by size: 3795 2081 47 StuI AGG'CCT Cuts at: 1517 2867 1517 Size: 1350 4573 StyI C'CwwG_G Cuts at: 513 1065 2748 3362 513 Size: 552 1683 614 3074 Fragments arranged by size: 3074 1683 614 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2175 3950 3967 4120 4305 4750 325 Size: 1255 595 1775 17 153 185 445 1498 Fragments arranged by size: 1775 1498 1255 595 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4169 5881 96 Size: 274 80 33 51 1443 2192 1712 138 Fragments arranged by size: 2192 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2055 2087 5883 5915 2055 Size: 32 3796 32 2063 Fragments arranged by size: 3796 2063 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5923 XbaI T'CTAG_A Cuts at: 3259 3259 Size: 5923 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5923 XmaI C'CCGG_G Cuts at: 1870 3248 1870 Size: 1378 4545 XmnI GAAnn'nnTTC Cuts at: 2169 4052 2169 Size: 1883 4040 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3731 276 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoNI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI