Restriction map for
pFN21AE1881
(Linear) (Six-base) MAPSORT of: KIEE1881.seq Check: 1543 from: 1 to: 1218
KIEE1881.orf
With 182 enzymes: SgfI *
July 15, 2010 18:49 ..
AclI AA'CG_TT
Cuts at: 0 45 1218
Size: 45 1173
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 425 956 1218
Size: 158 267 531 262
Fragments arranged by size:
531 267 262 158
AflIII A'CryG_T
Cuts at: 0 486 1218
Size: 486 732
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 570 1218
Size: 570 648
AlwNI CAG_nnn'CTG
Cuts at: 0 1179 1218
Size: 1179 39
ApoI r'AATT_y
Cuts at: 0 796 1204 1218
Size: 796 408 14
Fragments arranged by size:
796 408 14
BanII G_rGCy'C
Cuts at: 0 911 1190 1218
Size: 911 279 28
Fragments arranged by size:
911 279 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 245 1218
Size: 245 973
BglI GCCn_nnn'nGGC
Cuts at: 0 859 1218
Size: 859 359
Bme1580I G_kGCm'C
Cuts at: 0 590 1218
Size: 590 628
BmrI ACTGGGnnnn_n'
Cuts at: 0 1062 1218
Size: 1062 156
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 205 1218
Size: 205 1013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 118 268 1218
Size: 77 41 150 950
Fragments arranged by size:
950 150 77 41
Bpu10I CC'TnA_GC
Cuts at: 0 385 1218
Size: 385 833
BsaAI yAC'GTr
Cuts at: 0 114 1218
Size: 114 1104
BsaWI w'CCGG_w
Cuts at: 0 529 1218
Size: 529 689
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 50 71 80 101 1218
Size: 50 21 9 21 1117
Fragments arranged by size:
1117 50 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 374 949 1218
Size: 374 575 269
Fragments arranged by size:
575 374 269
BseYI C'CCAG_C
Cuts at: 0 989 1115 1218
Size: 989 126 103
Fragments arranged by size:
989 126 103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 1218
Size: 623 595
BsiHKAI G_wGCw'C
Cuts at: 0 155 1190 1218
Size: 155 1035 28
Fragments arranged by size:
1035 155 28
Bsp1286I G_dGCh'C
Cuts at: 0 155 590 911 1190 1218
Size: 155 435 321 279 28
Fragments arranged by size:
435 321 279 155 28
BspHI T'CATG_A
Cuts at: 0 689 1218
Size: 689 529
BsrFI r'CCGG_y
Cuts at: 0 1079 1218
Size: 1079 139
BstAPI GCAn_nnn'nTGC
Cuts at: 0 585 1218
Size: 585 633
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1115 1218
Size: 1115 103
Bsu36I CC'TnA_GG
Cuts at: 0 1074 1218
Size: 1074 144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 300 1218
Size: 300 918
BtsI GCAGTG_nn'
Cuts at: 0 611 1218
Size: 611 607
ClaI AT'CG_AT
Cuts at: 0 785 1218
Size: 785 433
DraI TTT'AAA
Cuts at: 0 50 674 1218
Size: 50 624 544
Fragments arranged by size:
624 544 50
EaeI y'GGCC_r
Cuts at: 0 1081 1218
Size: 1081 137
EarI CTCTTCn'nnn_
Cuts at: 0 97 1218
Size: 97 1121
EcoICRI GAG'CTC
Cuts at: 0 1188 1218
Size: 1188 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 118 158 268 425 956 1218
Size: 77 41 40 110 157 531 262
Fragments arranged by size:
531 262 157 110 77 41 40
EcoO109I rG'GnC_Cy
Cuts at: 0 124 1218
Size: 124 1094
EcoRI G'AATT_C
Cuts at: 0 796 1218
Size: 796 422
EcoRV GAT'ATC
Cuts at: 0 281 1218
Size: 281 937
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 717 1218
Size: 717 501
FseI GG_CCGG'CC
Cuts at: 0 1083 1218
Size: 1083 135
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 71 103 265 297 617 649 1218
Size: 71 32 162 32 320 32 569
Fragments arranged by size:
569 320 162 71 32 32 32
MfeI C'AATT_G
Cuts at: 0 926 1218
Size: 926 292
MslI CAynn'nnrTG
Cuts at: 0 640 1218
Size: 640 578
MspA1I CmG'CkG
Cuts at: 0 989 1179 1218
Size: 989 190 39
Fragments arranged by size:
989 190 39
NaeI GCC'GGC
Cuts at: 0 1081 1218
Size: 1081 137
NgoMIV G'CCGG_C
Cuts at: 0 1079 1218
Size: 1079 139
NspI r_CATG'y
Cuts at: 0 1011 1218
Size: 1011 207
PasI CC'CwG_GG
Cuts at: 0 1022 1218
Size: 1022 196
PflMI CCAn_nnn'nTGG
Cuts at: 0 10 744 833 1022 1218
Size: 10 734 89 189 196
Fragments arranged by size:
734 196 189 89 10
PpuMI rG'GwC_Cy
Cuts at: 0 124 1218
Size: 124 1094
PvuII CAG'CTG
Cuts at: 0 989 1179 1218
Size: 989 190 39
Fragments arranged by size:
989 190 39
SacI G_AGCT'C
Cuts at: 0 1190 1218
Size: 1190 28
SnaBI TAC'GTA
Cuts at: 0 114 1218
Size: 114 1104
StuI AGG'CCT
Cuts at: 0 862 1218
Size: 862 356
StyI C'CwwG_G
Cuts at: 0 743 1218
Size: 743 475
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 170 1218
Size: 170 1048
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 50 82 1218
Size: 50 32 1136
Fragments arranged by size:
1136 50 32
XmnI GAAnn'nnTTC
Cuts at: 0 164 1218
Size: 164 1054
Enzymes that do cut:
AclI AcuI AflIII AlfI AlwNI ApoI BanII BbsI
BglI Bme1580I BmrI BplI BpmI Bpu10I BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BsrFI
BstAPI BstXI Bsu36I BtgZI BtsI ClaI DraI EaeI
EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FseI
Hin4I MfeI MslI MspA1I NaeI NgoMIV NspI PasI
PflMI PpuMI PvuII SacI SnaBI StuI StyI TaqII
TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmtI BpuEI BsaI
BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BspEI BspMI
BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstYI
BstZ17I BtgI CspCI DraIII DrdI EagI EciI EcoNI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MluI MmeI MscI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
SrfI SspI SwaI TatI Tth111I XbaI XcmI XhoI
XmaI ZraI