################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BsaI BseAI BsiWI BsmAI BsmBI BspDI BspEI BsrBI BsrDI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraIII Eam1105I Eco47III Eco81I EcoNI EcoRI EcoT22I FbaI FseI FspI KpnI KspI MfeI MluI MroI MunI NaeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI Sse8387I StuI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AcyI 71 GGCGCC G[GA]^CG[CT]C AflIII 1706 ACATGT A^C[GA][CT]GT ApaI 1147 GGGCCC GGGCC^C AvaI 67 CCCGGG C^[CT]CG[GA]G BalI 203 TGGCCA TGG^CCA BbeI 71 GGCGCC GGCGC^C BbsI 2309 GAAGAC GAAGAC(2/6) BglII 1941 AGATCT A^GATCT BlpI 1735 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1735 GCTGAGC GC^T[ACGT]AGC BpuAI 2309 GAAGAC GAAGAC(2/6) BsaAI 1116 CACGTA [CT]AC^GT[GA] BsaHI 71 GGCGCC G[GA]^CG[CT]C BsaWI 798 TCCGGT [AT]^CCGG[AT] BseRI 2110 GAGGAG GAGGAG(10/8) BsgI 647 CTGCAC GTGCAG(16/14) BsiEI 90 CGGCCG CG[GA][CT]^CG BsoBI 67 CCCGGG C^[CT]CG[GA]G Bsp1407I 2457 TGTACA T^GTACA BspLU11I 1706 ACATGT A^CATGT BspMI 277 GCAGGT ACCTGC(4/8) BsrFI 2564 ACCGGC [GA]^CCGG[CT] BsrGI 2457 TGTACA T^GTACA BssHII 14 GCGCGC G^CGCGC BssSI 755 CTCGTG CACGAG(-5/-1) BstEII 972 GGTCACC G^GT[ACGT]ACC BstPI 972 GGTCACC G^GT[ACGT]ACC CelII 1735 GCTGAGC GC^T[ACGT]AGC Cfr10I 2564 ACCGGC [GA]^CCGG[CT] DrdI 964 GACTCCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 90 CGGCCG C^GGCCG EclXI 90 CGGCCG C^GGCCG Eco52I 90 CGGCCG C^GGCCG EcoO65I 972 GGTCACC G^GT[ACGT]ACC EcoRV 2019 GATATC GAT^ATC HgaI 2086 GACGC GACGC(5/10) Hin1I 71 GGCGCC G[GA]^CG[CT]C HincII 1710 GTTAAC GT[CT]^[GA]AC HindII 1710 GTTAAC GT[CT]^[GA]AC HindIII 845 AAGCTT A^AGCTT HpaI 1710 GTTAAC GTT^AAC KasI 71 GGCGCC G^GCGCC MluNI 203 TGGCCA TGG^CCA MscI 203 TGGCCA TGG^CCA NarI 71 GGCGCC GG^CGCC PflMI 991 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1916 AGGTCCT [GA]G^G[AT]CC[CT] PvuII 19 CAGCTG CAG^CTG SacI 247 GAGCTC GAGCT^C SfoI 71 GGCGCC GGC^GCC SmaI 67 CCCGGG CCC^GGG SphI 468 GCATGC GCATG^C SspBI 2457 TGTACA T^GTACA SwaI 438 ATTTAAAT ATTT^AAAT Van91I 991 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1023 TCTAGA T^CTAGA XcmI 1640 CCATTAGACTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 67 CCCGGG C^CCGGG XmaI 67 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 15 CGCG CG^CG 77 CGCG AcsI 436 GAATTT [GA]^AATT[CT] 503 AAATTC ApoI 436 GAATTT [GA]^AATT[CT] 503 AAATTC AspHI 247 GAGCTC G[AT]GC[AT]^C 868 GTGCTC BamHI 1241 GGATCC G^GATCC 1281 GGATCC BanI 71 GGCGCC G^G[CT][GA]CC 628 GGTGCC BsiHKAI 247 GAGCTC G[AT]GC[AT]^C 868 GTGCTC BspHI 1803 TCATGA T^CATGA 2093 TCATGA BstAPI 18 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 601 GCACTGGCTGC BstUI 15 CGCG CG^CG 77 CGCG DraI 439 TTTAAA TTT^AAA 636 TTTAAA DsaI 1055 CCATGG C^C[GA][CT]GG 1448 CCATGG EaeI 90 CGGCCG [CT]^GGCC[GA] 203 TGGCCA EarI 2060 GAAGAG CTCTTC(1/4) 2430 GAAGAG HaeII 71 GGCGCC [GA]GCGC^[CT] 2076 GGCGCT Ksp632I 2060 GAAGAG CTCTTC(1/4) 2430 GAAGAG MaeII 692 ACGT A^CGT 1117 ACGT MspA1I 19 CAGCTG C[AC]G^C[GT]G 52 CCGCTG MvnI 15 CGCG CG^CG 77 CGCG NcoI 1055 CCATGG C^CATGG 1448 CCATGG NspI 468 GCATGC [GA]CATG^[CT] 1706 ACATGT RcaI 1803 TCATGA T^CATGA 2093 TCATGA SspI 1357 AATATT AAT^ATT 2228 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI Asp700I AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaBI BsaJI BsiYI BslI BsmFI BsmI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MamI MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI