Restriction map for
pFN21AE6737
(Linear) (Six-base) MAPSORT of: KIEE6737.seq Check: 7512 from: 1 to: 2598
KIEE6737.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 857 1906 1958 2154 2554 2598
Size: 857 1049 52 196 400 44
Fragments arranged by size:
1049 857 400 196 52 44
AflIII A'CryG_T
Cuts at: 0 1706 2598
Size: 1706 892
AleI CACnn'nnGTG
Cuts at: 0 1211 2598
Size: 1211 1387
AlwNI CAG_nnn'CTG
Cuts at: 0 24 1238 1929 2177 2598
Size: 24 1214 691 248 421
Fragments arranged by size:
1214 691 421 248 24
ApaI G_GGCC'C
Cuts at: 0 1151 2598
Size: 1151 1447
ApoI r'AATT_y
Cuts at: 0 436 503 2598
Size: 436 67 2095
Fragments arranged by size:
2095 436 67
AvaI C'yCGr_G
Cuts at: 0 67 2598
Size: 67 2531
BamHI G'GATC_C
Cuts at: 0 1241 1281 2598
Size: 1241 40 1317
Fragments arranged by size:
1317 1241 40
BanI G'GyrC_C
Cuts at: 0 71 628 2598
Size: 71 557 1970
Fragments arranged by size:
1970 557 71
BanII G_rGCy'C
Cuts at: 0 64 251 756 1151 1421 1558 2598
Size: 64 187 505 395 270 137 1040
Fragments arranged by size:
1040 505 395 270 187 137 64
BbeI G_GCGC'C
Cuts at: 0 75 2598
Size: 75 2523
BbsI GAAGACnn'nnnn_
Cuts at: 0 2316 2598
Size: 2316 282
BglII A'GATC_T
Cuts at: 0 1941 2598
Size: 1941 657
BlpI GC'TnA_GC
Cuts at: 0 1736 2598
Size: 1736 862
Bme1580I G_kGCm'C
Cuts at: 0 633 1151 2598
Size: 633 518 1447
Fragments arranged by size:
1447 633 518
BmrI ACTGGGnnnn_n'
Cuts at: 0 1182 1308 2598
Size: 1182 126 1290
Fragments arranged by size:
1290 1182 126
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1418 2598
Size: 1418 1180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 584 974 1453 2179 2598
Size: 256 328 390 479 726 419
Fragments arranged by size:
726 479 419 390 328 256
BsaAI yAC'GTr
Cuts at: 0 1118 2598
Size: 1118 1480
BsaBI GATnn'nnATC
Cuts at: 0 1025 1286 2204 2345 2598
Size: 1025 261 918 141 253
Fragments arranged by size:
1025 918 261 253 141
BsaHI Gr'CG_yC
Cuts at: 0 72 2598
Size: 72 2526
BsaWI w'CCGG_w
Cuts at: 0 798 2598
Size: 798 1800
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1107 1137 1426 1456 2598
Size: 1107 30 289 30 1142
Fragments arranged by size:
1142 1107 289 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2125 2598
Size: 2125 473
BseYI C'CCAG_C
Cuts at: 0 610 1390 1466 1897 2037 2514 2598
Size: 610 780 76 431 140 477 84
Fragments arranged by size:
780 610 477 431 140 84 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 632 2598
Size: 632 1966
BsiEI CG_ry'CG
Cuts at: 0 93 2598
Size: 93 2505
BsiHKAI G_wGCw'C
Cuts at: 0 251 872 2598
Size: 251 621 1726
Fragments arranged by size:
1726 621 251
BsmI GAATG_Cn'
Cuts at: 0 741 2103 2151 2598
Size: 741 1362 48 447
Fragments arranged by size:
1362 741 447 48
Bsp1286I G_dGCh'C
Cuts at: 0 64 251 633 756 872 1151 1421 1558
Size: 64 187 382 123 116 279 270 137
Cuts at: 1558 2598
Size: 1040
Fragments arranged by size:
1040 382 279 270 187 137 123 116
64
BspHI T'CATG_A
Cuts at: 0 1803 2093 2598
Size: 1803 290 505
Fragments arranged by size:
1803 505 290
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 268 2598
Size: 268 2330
BsrFI r'CCGG_y
Cuts at: 0 2564 2598
Size: 2564 34
BsrGI T'GTAC_A
Cuts at: 0 2457 2598
Size: 2457 141
BssHII G'CGCG_C
Cuts at: 0 14 2598
Size: 14 2584
BssSI C'ACGA_G
Cuts at: 0 755 2598
Size: 755 1843
BstAPI GCAn_nnn'nTGC
Cuts at: 0 24 607 2598
Size: 24 583 1991
Fragments arranged by size:
1991 583 24
BstEII G'GTnAC_C
Cuts at: 0 972 2598
Size: 972 1626
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1062 1213 1762 2598
Size: 1062 151 549 836
Fragments arranged by size:
1062 836 549 151
BstYI r'GATC_y
Cuts at: 0 259 1241 1281 1941 2598
Size: 259 982 40 660 657
Fragments arranged by size:
982 660 657 259 40
BtgI C'CryG_G
Cuts at: 0 1055 1448 2598
Size: 1055 393 1150
Fragments arranged by size:
1150 1055 393
BtsI GCAGTG_nn'
Cuts at: 0 2006 2598
Size: 2006 592
DraI TTT'AAA
Cuts at: 0 441 638 2598
Size: 441 197 1960
Fragments arranged by size:
1960 441 197
DrdI GACnn_nn'nnGTC
Cuts at: 0 970 2598
Size: 970 1628
EaeI y'GGCC_r
Cuts at: 0 90 203 2598
Size: 90 113 2395
Fragments arranged by size:
2395 113 90
EagI C'GGCC_G
Cuts at: 0 90 2598
Size: 90 2508
EarI CTCTTCn'nnn_
Cuts at: 0 2055 2425 2598
Size: 2055 370 173
Fragments arranged by size:
2055 370 173
EcoICRI GAG'CTC
Cuts at: 0 249 2598
Size: 249 2349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 584 857 974 1453 1906 1958 2154
Size: 256 328 273 117 479 453 52 196
Cuts at: 2154 2179 2554 2598
Size: 25 375 44
Fragments arranged by size:
479 453 375 328 273 256 196 117
52 44 25
EcoO109I rG'GnC_Cy
Cuts at: 0 30 1147 1148 1917 2598
Size: 30 1117 1 769 681
Fragments arranged by size:
1117 769 681 30 1
EcoRV GAT'ATC
Cuts at: 0 2021 2598
Size: 2021 577
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1026 2598
Size: 1026 1572
HaeII r_GCGC'y
Cuts at: 0 75 2080 2598
Size: 75 2005 518
Fragments arranged by size:
2005 518 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1734 1766 2598
Size: 1734 32 832
Fragments arranged by size:
1734 832 32
HincII GTy'rAC
Cuts at: 0 1712 2598
Size: 1712 886
HindIII A'AGCT_T
Cuts at: 0 845 2598
Size: 845 1753
HpaI GTT'AAC
Cuts at: 0 1712 2598
Size: 1712 886
KasI G'GCGC_C
Cuts at: 0 71 2598
Size: 71 2527
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 924 2018 2598
Size: 924 1094 580
Fragments arranged by size:
1094 924 580
MscI TGG'CCA
Cuts at: 0 205 2598
Size: 205 2393
MslI CAynn'nnrTG
Cuts at: 0 812 1060 1211 2598
Size: 812 248 151 1387
Fragments arranged by size:
1387 812 248 151
MspA1I CmG'CkG
Cuts at: 0 21 54 2598
Size: 21 33 2544
Fragments arranged by size:
2544 33 21
NarI GG'CG_CC
Cuts at: 0 72 2598
Size: 72 2526
NcoI C'CATG_G
Cuts at: 0 1055 1448 2598
Size: 1055 393 1150
Fragments arranged by size:
1150 1055 393
NdeI CA'TA_TG
Cuts at: 0 933 1344 2448 2598
Size: 933 411 1104 150
Fragments arranged by size:
1104 933 411 150
NspI r_CATG'y
Cuts at: 0 472 1710 2598
Size: 472 1238 888
Fragments arranged by size:
1238 888 472
PciI A'CATG_T
Cuts at: 0 1706 2598
Size: 1706 892
PflMI CCAn_nnn'nTGG
Cuts at: 0 997 2598
Size: 997 1601
PfoI T'CCnGG_A
Cuts at: 0 300 561 2598
Size: 300 261 2037
Fragments arranged by size:
2037 300 261
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 470 502 776 808 2598
Size: 470 32 274 32 1790
Fragments arranged by size:
1790 470 274 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1917 2598
Size: 1917 681
PspOMI G'GGCC_C
Cuts at: 0 1147 2598
Size: 1147 1451
PstI C_TGCA'G
Cuts at: 0 59 126 744 2598
Size: 59 67 618 1854
Fragments arranged by size:
1854 618 67 59
PvuII CAG'CTG
Cuts at: 0 21 2598
Size: 21 2577
SacI G_AGCT'C
Cuts at: 0 251 2598
Size: 251 2347
SfcI C'TryA_G
Cuts at: 0 55 122 740 1181 2412 2418 2598
Size: 55 67 618 441 1231 6 180
Fragments arranged by size:
1231 618 441 180 67 55 6
SfoI GGC'GCC
Cuts at: 0 73 2598
Size: 73 2525
SmaI CCC'GGG
Cuts at: 0 69 2598
Size: 69 2529
SphI G_CATG'C
Cuts at: 0 472 2598
Size: 472 2126
SspI AAT'ATT
Cuts at: 0 1359 2230 2598
Size: 1359 871 368
Fragments arranged by size:
1359 871 368
StyI C'CwwG_G
Cuts at: 0 1055 1448 1718 2294 2327 2598
Size: 1055 393 270 576 33 271
Fragments arranged by size:
1055 576 393 271 270 33
SwaI ATTT'AAAT
Cuts at: 0 441 2598
Size: 441 2157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1164 1731 2563 2598
Size: 1164 567 832 35
Fragments arranged by size:
1164 832 567 35
TatI w'GTAC_w
Cuts at: 0 1316 1511 2457 2598
Size: 1316 195 946 141
Fragments arranged by size:
1316 946 195 141
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 612 644 1107 1139 1426 1458 2468 2500
Size: 612 32 463 32 287 32 1010 32
Cuts at: 2500 2598
Size: 98
Fragments arranged by size:
1010 612 463 287 98 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 1023 2598
Size: 1023 1575
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1647 2598
Size: 1647 951
XmaI C'CCGG_G
Cuts at: 0 67 2598
Size: 67 2531
XmnI GAAnn'nnTTC
Cuts at: 0 503 1064 2167 2598
Size: 503 561 1103 431
Fragments arranged by size:
1103 561 503 431
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbeI BbsI BglII BlpI Bme1580I BmrI
BplI BpmI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI
BtgI BtsI DraI DrdI EaeI EagI EarI EcoICRI
Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII
HpaI KasI MmeI MscI MslI MspA1I NarI NcoI
NdeI NspI PciI PflMI PfoI PpiI PpuMI PspOMI
PstI PvuII SacI SfcI SfoI SmaI SphI SspI
StyI SwaI TaqII TatI TstI XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BbvCI BcgI BciVI BclI BfrBI BglI BmgBI
BmtI Bpu10I BpuEI BsaI BsiWI BsmBI BspEI BsrBI
BsrDI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII
EciI EcoNI EcoRI FseI FspI FspAI KpnI MfeI
MluI NaeI NgoMIV NheI NotI NruI NsiI PacI
PasI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI StuI
Tth111I XhoI ZraI