Restriction map for
pFN21AE2453
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
AccI AccIII AclI AcsI AflII
AgeI AhdI AocI Aor51HI ApoI
AscI AseI AsnI AspEI AspI
AvrII BciVI BfrI BglI BlnI
BlpI Bpu1102I BsaI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspEI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I CpoI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI FseI HindIII HpaI KspI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI PacI
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tth111I XcmI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 1864 GACGTC GACGT^C
Acc65I 1477 GGTACC G^GTACC
Alw44I 1579 GTGCAC G^TGCAC
AlwNI 2259 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1579 GTGCAC G^TGCAC
Asp718I 1477 GGTACC G^GTACC
AviII 359 TGCGCA TGC^GCA
BamHI 1328 GGATCC G^GATCC
BbeI 281 GGCGCC GGCGC^C
BbvCI 1468 CCTCAGC CCTCAGC(-5/-2)
BglII 2498 AGATCT A^GATCT
BsaWI 2468 ACCGGA [AT]^CCGG[AT]
BspDI 1263 ATCGAT AT^CGAT
BsrBI 33 GAGCGG CCGCTC(-3/-3)
BstBI 892 TTCGAA TT^CGAA
ClaI 1263 ATCGAT AT^CGAT
DraI 1767 TTTAAA TTT^AAA
DraIII 619 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 489 GACTCCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1319 CCATGG C^C[GA][CT]GG
EcoNI 890 CCTTCGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1266 GATATC GAT^ATC
EcoT22I 999 ATGCAT ATGCA^T
FspI 359 TGCGCA TGC^GCA
HincII 880 GTCAAC GT[CT]^[GA]AC
HindII 880 GTCAAC GT[CT]^[GA]AC
KasI 281 GGCGCC G^GCGCC
KpnI 1477 GGTACC GGTAC^C
NarI 281 GGCGCC GG^CGCC
NcoI 1319 CCATGG C^CATGG
NruI 643 TCGCGA TCG^CGA
NsiI 999 ATGCAT ATGCA^T
NspV 892 TTCGAA TT^CGAA
PaeR7I 1274 CTCGAG C^TCGAG
SacI 922 GAGCTC GAGCT^C
SfoI 281 GGCGCC GGC^GCC
SfuI 892 TTCGAA TT^CGAA
SmaI 1977 CCCGGG CCC^GGG
StuI 1518 AGGCCT AGG^CCT
XbaI 1434 TCTAGA T^CTAGA
XhoI 1274 CTCGAG C^TCGAG
XmaCI 1977 CCCGGG C^CCGGG
XmaI 1977 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AflIII 2202 ACATGT A^C[GA][CT]GT
2528 ACATGT
BalI 1095 TGGCCA TGG^CCA
2345 TGGCCA
BbrPI 865 CACGTG CAC^GTG
1072 CACGTG
BcgI 634 GCAGTGGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1299 GCAGAATCGTCG
BclI 546 TGATCA T^GATCA
2312 TGATCA
BfaI 1435 CTAG C^TAG
2611 CTAG
BpmI 491 CTCCAG CTGGAG(16/14)
2050 CTGGAG
BsaAI 865 CACGTG [CT]AC^GT[GA]
1072 CACGTG
BsaBI 520 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1486 GATGAAGATC
BseRI 1660 GAGGAG GAGGAG(10/8)
2401 GAGGAG
BsgI 1870 CTGCAC GTGCAG(16/14)
585 GTGCAG
BsmI 508 GAATGC GAATGC(1/-1)
2269 GAATGC
BspHI 528 TCATGA T^CATGA
834 TCATGA
BspLU11I 2202 ACATGT A^CATGT
2528 ACATGT
EaeI 1095 TGGCCA [CT]^GGCC[GA]
2345 TGGCCA
FbaI 546 TGATCA T^GATCA
2312 TGATCA
HaeII 281 GGCGCC [GA]GCGC^[CT]
298 AGCGCC
MaeI 1435 CTAG C^TAG
2611 CTAG
MamI 520 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1486 GATGAAGATC
MluNI 1095 TGGCCA TGG^CCA
2345 TGGCCA
MscI 1095 TGGCCA TGG^CCA
2345 TGGCCA
PflMI 1087 CCAGTGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1962 CCATGACGTGG
PmaCI 865 CACGTG CAC^GTG
1072 CACGTG
PmlI 865 CACGTG CAC^GTG
1072 CACGTG
PpuMI 177 GGGACCC [GA]G^G[AT]CC[CT]
386 AGGACCC
PstI 291 CTGCAG CTGCA^G
1297 CTGCAG
PvuII 1471 CAGCTG CAG^CTG
2243 CAGCTG
RcaI 528 TCATGA T^CATGA
834 TCATGA
SspI 478 AATATT AAT^ATT
2119 AATATT
Van91I 1087 CCAGTGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1962 CCATGACGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI Asp700I AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BmyI BpuAI BsaHI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI