(Circular) (Six-base) MAPSORT of: pFN21AE2453.seq Check: 7236 from: 1 to: 7360 pFN21AE2453 7360 bp With 182 enzymes: SgfI * February 8, 2013 19:21 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2335 2335 Size: 7360 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3873 5170 278 Size: 53 83 186 936 2337 1297 2468 Fragments arranged by size: 2468 2337 1297 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4703 1937 Size: 2766 4594 Acc65I G'GTAC_C Cuts at: 3482 4681 3482 Size: 1199 6161 AclI AA'CG_TT Cuts at: 1563 5487 5860 1563 Size: 3924 373 3063 Fragments arranged by size: 3924 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5423 6807 7075 1632 Size: 3791 1384 268 1917 Fragments arranged by size: 3791 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7163 AflIII A'CryG_T Cuts at: 1203 4207 4533 6259 7236 1203 Size: 3004 326 1726 977 1327 Fragments arranged by size: 3004 1726 1327 977 326 AgeI A'CCGG_T Cuts at: 6191 6191 Size: 7360 AhdI GACnn_n'nnGTC Cuts at: 6089 6089 Size: 7360 AleI CACnn'nnGTG Cuts at: 1166 2096 2336 2922 3092 3507 1166 Size: 930 240 586 170 415 5019 Fragments arranged by size: 5019 930 586 415 240 170 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7360 AlwNI CAG_nnn'CTG Cuts at: 4269 5103 6675 4269 Size: 834 1572 4954 Fragments arranged by size: 4954 1572 834 ApaI G_GGCC'C Cuts at: 2111 2215 2376 2111 Size: 104 161 7095 Fragments arranged by size: 7095 161 104 ApaLI G'TGCA_C Cuts at: 3584 5417 6573 3584 Size: 1833 1156 4371 Fragments arranged by size: 4371 1833 1156 ApoI r'AATT_y Cuts at: 1493 4669 4895 5047 7156 1493 Size: 3176 226 152 2109 1697 Fragments arranged by size: 3176 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5914 160 Size: 872 4882 1606 Fragments arranged by size: 4882 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7360 AvaI C'yCGr_G Cuts at: 1870 1943 2167 3279 3982 4685 1870 Size: 73 224 1112 703 703 4545 Fragments arranged by size: 4545 1112 703 703 224 73 BamHI G'GATC_C Cuts at: 3333 4690 5057 3333 Size: 1357 367 5636 Fragments arranged by size: 5636 1357 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2116 2286 3482 4681 6136 Size: 325 217 627 329 170 1196 1199 1455 Cuts at: 6136 618 Size: 1842 Fragments arranged by size: 1842 1455 1199 1196 627 329 325 217 170 BanII G_rGCy'C Cuts at: 1391 2111 2215 2376 2931 4679 1391 Size: 720 104 161 555 1748 4072 Fragments arranged by size: 4072 1748 720 555 161 104 BbeI G_GCGC'C Cuts at: 2290 2290 Size: 7360 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2343 3553 3736 4616 928 Size: 971 444 1210 183 880 3672 Fragments arranged by size: 3672 1210 971 880 444 183 BbvCI CC'TCA_GC Cuts at: 3474 3474 Size: 7360 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2628 2662 3293 3327 5551 5585 1903 Size: 34 691 34 631 34 2224 34 3678 Fragments arranged by size: 3678 2224 691 631 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5252 6462 5252 Size: 1210 6150 BclI T'GATC_A Cuts at: 1548 2551 4317 1548 Size: 1003 1766 4591 Fragments arranged by size: 4591 1766 1003 BfrBI ATG'CAT Cuts at: 3006 3006 Size: 7360 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5971 136 Size: 107 122 71 631 605 4299 1525 Fragments arranged by size: 4299 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4503 7355 4503 Size: 2852 4508 BlpI GC'TnA_GC Cuts at: 4777 4777 Size: 7360 Bme1580I G_kGCm'C Cuts at: 1790 2111 2215 2376 3588 5421 6577 1790 Size: 321 104 161 1212 1833 1156 2573 Fragments arranged by size: 2573 1833 1212 1156 321 161 104 BmgBI CAC'GTC Cuts at: 1318 3262 3973 4348 1318 Size: 1944 711 375 4330 Fragments arranged by size: 4330 1944 711 375 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2782 3086 3401 Size: 416 53 83 399 24 1365 304 315 Cuts at: 3401 5091 6049 442 Size: 1690 958 1753 Fragments arranged by size: 1753 1690 1365 958 416 399 315 304 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7360 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2092 2092 Size: 7360 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2481 4076 6020 1239 Size: 1242 1595 1944 2579 Fragments arranged by size: 2579 1944 1595 1242 Bpu10I CC'TnA_GC Cuts at: 3474 3474 Size: 7360 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3119 3562 5485 6350 6648 3119 Size: 443 1923 865 298 3831 Fragments arranged by size: 3831 1923 865 443 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6023 882 Size: 617 4524 2219 Fragments arranged by size: 4524 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2872 3079 5151 493 Size: 711 1668 207 2072 2702 Fragments arranged by size: 2702 2072 1668 711 207 BsaBI GATnn'nnATC Cuts at: 2529 3495 7354 2529 Size: 966 3859 2535 Fragments arranged by size: 3859 2535 966 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2148 2157 2287 2675 Size: 53 83 186 936 615 9 130 388 Cuts at: 2675 3870 5167 5549 6184 275 Size: 1195 1297 382 635 1451 Fragments arranged by size: 1451 1297 1195 936 635 615 388 382 186 130 83 53 9 BsaWI w'CCGG_w Cuts at: 4473 5793 6191 6465 6612 7023 4473 Size: 1320 398 274 147 411 4810 Fragments arranged by size: 4810 1320 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3836 3866 1178 Size: 30 165 30 2433 30 4672 Fragments arranged by size: 4672 2433 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3680 4421 1186 Size: 2494 741 4125 Fragments arranged by size: 4125 2494 741 BseYI C'CCAG_C Cuts at: 2018 2189 2224 2890 3478 4286 4391 6563 7059 Size: 171 35 666 588 808 105 2172 496 Cuts at: 7059 2018 Size: 2319 Fragments arranged by size: 2319 2172 808 666 588 496 171 105 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2611 3860 1163 Size: 573 875 1249 4663 Fragments arranged by size: 4663 1249 875 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4810 5571 6599 1540 Size: 275 186 2809 761 1028 2301 Fragments arranged by size: 2809 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2625 2931 3588 4035 5421 5506 6577 2625 Size: 306 657 447 1386 85 1071 3408 Fragments arranged by size: 3408 1386 1071 657 447 306 85 BsmI GAATG_Cn' Cuts at: 2519 4280 4871 4964 2519 Size: 1761 591 93 4915 Fragments arranged by size: 4915 1761 591 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2111 2215 2376 2625 2931 3588 4035 Size: 399 321 104 161 249 306 657 447 Cuts at: 4035 4679 5421 5506 6577 1391 Size: 644 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 657 644 447 399 321 306 249 161 104 85 BspEI T'CCGG_A Cuts at: 7023 7023 Size: 7360 BspHI T'CATG_A Cuts at: 2533 2839 5249 6979 2533 Size: 306 2410 1730 2914 Fragments arranged by size: 2914 2410 1730 306 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2333 2335 2583 4715 844 Size: 1489 2 248 2132 3489 Fragments arranged by size: 3489 2132 1489 248 2 BsrBI CCG'CTC Cuts at: 1394 1823 2040 5247 1394 Size: 429 217 3207 3507 Fragments arranged by size: 3507 3207 429 217 BsrDI GCAATG_nn' Cuts at: 65 1248 3615 3951 4015 5855 6029 65 Size: 1183 2367 336 64 1840 174 1396 Fragments arranged by size: 2367 1840 1396 1183 336 174 64 BsrFI r'CCGG_y Cuts at: 1700 1810 6004 6191 1700 Size: 110 4194 187 2869 Fragments arranged by size: 4194 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7360 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7360 BssSI C'ACGA_G Cuts at: 5420 6432 5420 Size: 1012 6348 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7360 BstBI TT'CG_AA Cuts at: 2898 6164 2898 Size: 3266 4094 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7360 BstYI r'GATC_y Cuts at: 1972 3333 3496 4398 4503 4690 5057 5442 5459 Size: 1361 163 902 105 187 367 385 17 Cuts at: 5459 6911 6997 7009 7355 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1361 902 385 367 346 187 163 105 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3324 513 Size: 552 939 1320 4549 Fragments arranged by size: 4549 1320 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2454 2877 487 Size: 683 56 1228 423 4970 Fragments arranged by size: 4970 1228 683 423 56 BtsI GCAGTG_nn' Cuts at: 2290 2592 2646 3292 4024 4044 4876 5670 5690 Size: 302 54 646 732 20 832 794 20 Cuts at: 5690 2290 Size: 3960 Fragments arranged by size: 3960 832 794 732 646 302 54 20 20 ClaI AT'CG_AT Cuts at: 1554 3269 1554 Size: 1715 5645 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7325 DraI TTT'AAA Cuts at: 3774 4664 3774 Size: 890 6470 DraIII CAC_nnn'GTG Cuts at: 2629 2629 Size: 7360 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2500 6367 809 Size: 507 1184 3867 1802 Fragments arranged by size: 3867 1802 1184 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3100 4350 4807 Size: 54 1425 50 275 18 1270 1250 457 Cuts at: 4807 5696 7325 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1270 1250 889 457 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4807 1537 Size: 275 2995 4090 Fragments arranged by size: 4090 2995 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2138 2664 2694 3798 3838 4425 4430 5290 Size: 785 526 30 1104 40 587 5 860 Cuts at: 5290 1353 Size: 3423 Fragments arranged by size: 3423 1104 860 785 587 526 40 30 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5947 6321 6467 387 Size: 1064 138 4358 374 146 1280 Fragments arranged by size: 4358 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2929 2929 Size: 7360 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2481 4076 5423 6020 6807 7075 1239 Size: 393 849 1595 1347 597 787 268 1524 Fragments arranged by size: 1595 1524 1347 849 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2899 2899 Size: 7360 EcoO109I rG'GnC_Cy Cuts at: 2107 2183 2211 2392 2107 Size: 76 28 181 7075 Fragments arranged by size: 7075 181 76 28 EcoRI G'AATT_C Cuts at: 4669 5047 4669 Size: 378 6982 EcoRV GAT'ATC Cuts at: 3273 3273 Size: 7360 FspI TGC'GCA Cuts at: 2366 5866 2366 Size: 3500 3860 FspAI rTGC'GCAy Cuts at: 2366 2366 Size: 7360 HaeII r_GCGC'y Cuts at: 1678 2290 2307 6507 1678 Size: 612 17 4200 2531 Fragments arranged by size: 4200 2531 612 17 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3202 3234 3251 3262 3283 Size: 32 135 32 1630 32 17 11 21 Cuts at: 3283 3294 3583 3615 6001 6033 6075 6107 1373 Size: 11 289 32 2386 32 42 32 2626 Fragments arranged by size: 2626 2386 1630 289 135 42 32 32 32 32 32 32 21 17 11 11 HincII GTy'rAC Cuts at: 669 1657 1938 2887 4704 4950 669 Size: 988 281 949 1817 246 3079 Fragments arranged by size: 3079 1817 988 949 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7360 HpaI GTT'AAC Cuts at: 4950 4950 Size: 7360 KasI G'GCGC_C Cuts at: 2286 2286 Size: 7360 KpnI G_GTAC'C Cuts at: 3486 4685 3486 Size: 1199 6161 MfeI C'AATT_G Cuts at: 4959 4959 Size: 7360 MluI A'CGCG_T Cuts at: 7236 7236 Size: 7360 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3094 3499 3912 6474 6658 1305 Size: 1789 405 413 2562 184 2007 Fragments arranged by size: 2562 2007 1789 413 405 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3102 4352 10 Size: 54 1425 343 1270 1250 3018 Fragments arranged by size: 3018 1425 1270 1250 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2096 2336 2838 2922 3092 3260 3507 Size: 648 930 240 502 84 170 168 247 Cuts at: 3507 5318 5677 5836 518 Size: 1811 359 159 2042 Fragments arranged by size: 2042 1811 930 648 502 359 247 240 170 168 159 84 MspA1I CmG'CkG Cuts at: 1606 2232 3478 4250 4776 5453 6601 6846 1606 Size: 626 1246 772 526 677 1148 245 2120 Fragments arranged by size: 2120 1246 1148 772 677 626 526 245 NarI GG'CG_CC Cuts at: 2287 2287 Size: 7360 NcoI C'CATG_G Cuts at: 513 1065 2004 3324 513 Size: 552 939 1320 4549 Fragments arranged by size: 4549 1320 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7360 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7360 NotI GC'GGCC_GC Cuts at: 4807 4807 Size: 7360 NruI TCG'CGA Cuts at: 2650 2650 Size: 7360 NsiI A_TGCA'T Cuts at: 3008 3008 Size: 7360 NspI r_CATG'y Cuts at: 1132 1229 3006 4211 4537 4718 6263 1132 Size: 97 1777 1205 326 181 1545 2229 Fragments arranged by size: 2229 1777 1545 1205 326 181 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7360 PciI A'CATG_T Cuts at: 4207 4533 6259 4207 Size: 326 1726 5308 Fragments arranged by size: 5308 1726 326 PflMI CCAn_nnn'nTGG Cuts at: 1108 3098 3973 1108 Size: 1990 875 4495 Fragments arranged by size: 4495 1990 875 PmeI GTTT'AAAC Cuts at: 4664 4664 Size: 7360 PmlI CAC'GTG Cuts at: 2872 3079 2872 Size: 207 7153 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5429 5461 6942 6974 5429 Size: 32 1481 32 5815 Fragments arranged by size: 5815 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2183 2392 2183 Size: 209 7151 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7360 PsiI TTA'TAA Cuts at: 3374 4930 3374 Size: 1556 5804 PspOMI G'GGCC_C Cuts at: 2107 2211 2372 2107 Size: 104 161 7095 Fragments arranged by size: 7095 161 104 PstI C_TGCA'G Cuts at: 830 2300 3306 4712 830 Size: 1470 1006 1406 3478 Fragments arranged by size: 3478 1470 1406 1006 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7360 PvuII CAG'CTG Cuts at: 3478 4250 3478 Size: 772 6588 SacI G_AGCT'C Cuts at: 2931 2931 Size: 7360 SalI G'TCGA_C Cuts at: 1936 4702 1936 Size: 2766 4594 SanDI GG'GwC_CC Cuts at: 2183 2183 Size: 7360 SbfI CC_TGCA'GG Cuts at: 4712 4712 Size: 7360 ScaI AGT'ACT Cuts at: 5608 7320 5608 Size: 1712 5648 SfcI C'TryA_G Cuts at: 826 1045 2296 3302 4483 4708 5843 6524 6715 Size: 219 1251 1006 1181 225 1135 681 191 Cuts at: 6715 826 Size: 1471 Fragments arranged by size: 1471 1251 1181 1135 1006 681 225 219 191 SfoI GGC'GCC Cuts at: 2288 2288 Size: 7360 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7360 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7360 SmaI CCC'GGG Cuts at: 1872 3984 4687 1872 Size: 2112 703 4545 Fragments arranged by size: 4545 2112 703 SmlI C'TyrA_G Cuts at: 820 1017 1943 3134 3279 3541 5464 6365 6627 Size: 197 926 1191 145 262 1923 901 262 Cuts at: 6627 820 Size: 1553 Fragments arranged by size: 1923 1553 1191 926 901 262 262 197 145 SnaBI TAC'GTA Cuts at: 493 5151 493 Size: 4658 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7360 SphI G_CATG'C Cuts at: 1132 4718 1132 Size: 3586 3774 SspI AAT'ATT Cuts at: 5 52 2485 4126 5284 5 Size: 47 2433 1641 1158 2081 Fragments arranged by size: 2433 2081 1641 1158 47 StuI AGG'CCT Cuts at: 1517 3525 1517 Size: 2008 5352 StyI C'CwwG_G Cuts at: 513 1065 2004 3103 3324 3426 4368 4799 513 Size: 552 939 1099 221 102 942 431 3074 Fragments arranged by size: 3074 1099 942 939 552 431 221 102 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2159 3872 5387 5404 5557 5742 6187 Size: 1255 579 1713 1515 17 153 185 445 Cuts at: 6187 325 Size: 1498 Fragments arranged by size: 1713 1515 1498 1255 579 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3465 5606 7318 Size: 274 80 33 51 1443 1488 2141 1712 Cuts at: 7318 96 Size: 138 Fragments arranged by size: 2141 1712 1488 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4437 4469 7320 7352 4437 Size: 32 2851 32 4445 Fragments arranged by size: 4445 2851 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7360 XbaI T'CTAG_A Cuts at: 3439 4696 3439 Size: 1257 6103 XhoI C'TCGA_G Cuts at: 1943 3279 1943 Size: 1336 6024 XmaI C'CCGG_G Cuts at: 1870 3982 4685 1870 Size: 2112 703 4545 Fragments arranged by size: 4545 2112 703 XmnI GAAnn'nnTTC Cuts at: 2517 2757 4295 5489 2517 Size: 240 1538 1194 4388 Fragments arranged by size: 4388 1538 1194 240 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3871 5168 276 Size: 53 83 186 936 2337 1297 2468 Fragments arranged by size: 2468 2337 1297 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BsiWI BsmBI BstXI BstZ17I Bsu36I FalI FseI NaeI NgoMIV PacI PfoI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI XcmI