Restriction map for
pFN21AE2453
(Linear) (Six-base) MAPSORT of: KIEE2453.seq Check: 3128 from: 1 to: 2655
KIEE2453.orf
With 182 enzymes: SgfI *
February 8, 2013 18:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 330 2655
Size: 330 2325
AatII G_ACGT'C
Cuts at: 0 1868 2655
Size: 1868 787
Acc65I G'GTAC_C
Cuts at: 0 1477 2655
Size: 1477 1178
AflIII A'CryG_T
Cuts at: 0 2202 2528 2655
Size: 2202 326 127
Fragments arranged by size:
2202 326 127
AleI CACnn'nnGTG
Cuts at: 0 91 331 917 1087 1502 2655
Size: 91 240 586 170 415 1153
Fragments arranged by size:
1153 586 415 240 170 91
AlwNI CAG_nnn'CTG
Cuts at: 0 2264 2655
Size: 2264 391
ApaI G_GGCC'C
Cuts at: 0 106 210 371 2655
Size: 106 104 161 2284
Fragments arranged by size:
2284 161 106 104
ApaLI G'TGCA_C
Cuts at: 0 1579 2655
Size: 1579 1076
AvaI C'yCGr_G
Cuts at: 0 162 1274 1977 2655
Size: 162 1112 703 678
Fragments arranged by size:
1112 703 678 162
BamHI G'GATC_C
Cuts at: 0 1328 2655
Size: 1328 1327
BanI G'GyrC_C
Cuts at: 0 111 281 1477 2655
Size: 111 170 1196 1178
Fragments arranged by size:
1196 1178 170 111
BanII G_rGCy'C
Cuts at: 0 106 210 371 926 2655
Size: 106 104 161 555 1729
Fragments arranged by size:
1729 555 161 106 104
BbeI G_GCGC'C
Cuts at: 0 285 2655
Size: 285 2370
BbsI GAAGACnn'nnnn_
Cuts at: 0 338 1548 1731 2611 2655
Size: 338 1210 183 880 44
Fragments arranged by size:
1210 880 338 183 44
BbvCI CC'TCA_GC
Cuts at: 0 1469 2655
Size: 1469 1186
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 623 657 1288 1322 2655
Size: 623 34 631 34 1333
Fragments arranged by size:
1333 631 623 34 34
BclI T'GATC_A
Cuts at: 0 546 2312 2655
Size: 546 1766 343
Fragments arranged by size:
1766 546 343
BfrBI ATG'CAT
Cuts at: 0 1001 2655
Size: 1001 1654
BglII A'GATC_T
Cuts at: 0 2498 2655
Size: 2498 157
Bme1580I G_kGCm'C
Cuts at: 0 106 210 371 1583 2655
Size: 106 104 161 1212 1072
Fragments arranged by size:
1212 1072 161 106 104
BmgBI CAC'GTC
Cuts at: 0 1257 1968 2343 2655
Size: 1257 711 375 312
Fragments arranged by size:
1257 711 375 312
BmrI ACTGGGnnnn_n'
Cuts at: 0 777 1081 1396 2655
Size: 777 304 315 1259
Fragments arranged by size:
1259 777 315 304
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 87 2655
Size: 87 2568
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 2071 2655
Size: 476 1595 584
Fragments arranged by size:
1595 584 476
Bpu10I CC'TnA_GC
Cuts at: 0 1469 2655
Size: 1469 1186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1114 1557 2655
Size: 1114 443 1098
Fragments arranged by size:
1114 1098 443
BsaAI yAC'GTr
Cuts at: 0 867 1074 2655
Size: 867 207 1581
Fragments arranged by size:
1581 867 207
BsaBI GATnn'nnATC
Cuts at: 0 524 1490 2655
Size: 524 966 1165
Fragments arranged by size:
1165 966 524
BsaHI Gr'CG_yC
Cuts at: 0 143 152 282 670 1865 2655
Size: 143 9 130 388 1195 790
Fragments arranged by size:
1195 790 388 143 130 9
BsaWI w'CCGG_w
Cuts at: 0 2468 2655
Size: 2468 187
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1831 1861 2655
Size: 1831 30 794
Fragments arranged by size:
1831 794 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1675 2416 2655
Size: 1675 741 239
Fragments arranged by size:
1675 741 239
BseYI C'CCAG_C
Cuts at: 0 13 184 219 885 1473 2281 2386 2655
Size: 13 171 35 666 588 808 105 269
Fragments arranged by size:
808 666 588 269 171 105 35 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 606 1855 2655
Size: 606 1249 800
Fragments arranged by size:
1249 800 606
BsiHKAI G_wGCw'C
Cuts at: 0 620 926 1583 2030 2655
Size: 620 306 657 447 625
Fragments arranged by size:
657 625 620 447 306
BsmI GAATG_Cn'
Cuts at: 0 514 2275 2655
Size: 514 1761 380
Fragments arranged by size:
1761 514 380
Bsp1286I G_dGCh'C
Cuts at: 0 106 210 371 620 926 1583 2030 2655
Size: 106 104 161 249 306 657 447 625
Fragments arranged by size:
657 625 447 306 249 161 106 104
BspHI T'CATG_A
Cuts at: 0 528 834 2655
Size: 528 306 1821
Fragments arranged by size:
1821 528 306
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 328 330 578 2655
Size: 328 2 248 2077
Fragments arranged by size:
2077 328 248 2
BsrBI CCG'CTC
Cuts at: 0 35 2655
Size: 35 2620
BsrDI GCAATG_nn'
Cuts at: 0 1610 1946 2010 2655
Size: 1610 336 64 645
Fragments arranged by size:
1610 645 336 64
BstBI TT'CG_AA
Cuts at: 0 893 2655
Size: 893 1762
BstYI r'GATC_y
Cuts at: 0 1328 1491 2393 2498 2655
Size: 1328 163 902 105 157
Fragments arranged by size:
1328 902 163 157 105
BtgI C'CryG_G
Cuts at: 0 1319 2655
Size: 1319 1336
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 449 872 2655
Size: 449 423 1783
Fragments arranged by size:
1783 449 423
BtsI GCAGTG_nn'
Cuts at: 0 285 587 641 1287 2019 2039 2655
Size: 285 302 54 646 732 20 616
Fragments arranged by size:
732 646 616 302 285 54 20
ClaI AT'CG_AT
Cuts at: 0 1264 2655
Size: 1264 1391
DraI TTT'AAA
Cuts at: 0 1769 2655
Size: 1769 886
DraIII CAC_nnn'GTG
Cuts at: 0 624 2655
Size: 624 2031
DrdI GACnn_nn'nnGTC
Cuts at: 0 495 2655
Size: 495 2160
EaeI y'GGCC_r
Cuts at: 0 1095 2345 2655
Size: 1095 1250 310
Fragments arranged by size:
1250 1095 310
EarI CTCTTCn'nnn_
Cuts at: 0 133 659 689 1793 1833 2420 2425 2655
Size: 133 526 30 1104 40 587 5 230
Fragments arranged by size:
1104 587 526 230 133 40 30 5
EcoICRI GAG'CTC
Cuts at: 0 924 2655
Size: 924 1731
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 2071 2655
Size: 476 1595 584
Fragments arranged by size:
1595 584 476
EcoNI CCTnn'n_nnAGG
Cuts at: 0 894 2655
Size: 894 1761
EcoO109I rG'GnC_Cy
Cuts at: 0 102 178 206 387 2655
Size: 102 76 28 181 2268
Fragments arranged by size:
2268 181 102 76 28
EcoRV GAT'ATC
Cuts at: 0 1268 2655
Size: 1268 1387
FspI TGC'GCA
Cuts at: 0 361 2655
Size: 361 2294
FspAI rTGC'GCAy
Cuts at: 0 361 2655
Size: 361 2294
HaeII r_GCGC'y
Cuts at: 0 285 302 2655
Size: 285 17 2353
Fragments arranged by size:
2353 285 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1197 1229 1246 1257 1278 1289 1578 1610
Size: 1197 32 17 11 21 11 289 32
Cuts at: 1610 2655
Size: 1045
Fragments arranged by size:
1197 1045 289 32 32 21 17 11
11
HincII GTy'rAC
Cuts at: 0 882 2655
Size: 882 1773
KasI G'GCGC_C
Cuts at: 0 281 2655
Size: 281 2374
KpnI G_GTAC'C
Cuts at: 0 1481 2655
Size: 1481 1174
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1089 1494 1907 2655
Size: 1089 405 413 748
Fragments arranged by size:
1089 748 413 405
MscI TGG'CCA
Cuts at: 0 1097 2347 2655
Size: 1097 1250 308
Fragments arranged by size:
1250 1097 308
MslI CAynn'nnrTG
Cuts at: 0 91 331 833 917 1087 1255 1502 2655
Size: 91 240 502 84 170 168 247 1153
Fragments arranged by size:
1153 502 247 240 170 168 91 84
MspA1I CmG'CkG
Cuts at: 0 227 1473 2245 2655
Size: 227 1246 772 410
Fragments arranged by size:
1246 772 410 227
NarI GG'CG_CC
Cuts at: 0 282 2655
Size: 282 2373
NcoI C'CATG_G
Cuts at: 0 1319 2655
Size: 1319 1336
NruI TCG'CGA
Cuts at: 0 645 2655
Size: 645 2010
NsiI A_TGCA'T
Cuts at: 0 1003 2655
Size: 1003 1652
NspI r_CATG'y
Cuts at: 0 1001 2206 2532 2655
Size: 1001 1205 326 123
Fragments arranged by size:
1205 1001 326 123
PciI A'CATG_T
Cuts at: 0 2202 2528 2655
Size: 2202 326 127
Fragments arranged by size:
2202 326 127
PflMI CCAn_nnn'nTGG
Cuts at: 0 1093 1968 2655
Size: 1093 875 687
Fragments arranged by size:
1093 875 687
PmlI CAC'GTG
Cuts at: 0 867 1074 2655
Size: 867 207 1581
Fragments arranged by size:
1581 867 207
PpuMI rG'GwC_Cy
Cuts at: 0 178 387 2655
Size: 178 209 2268
Fragments arranged by size:
2268 209 178
PsiI TTA'TAA
Cuts at: 0 1369 2655
Size: 1369 1286
PspOMI G'GGCC_C
Cuts at: 0 102 206 367 2655
Size: 102 104 161 2288
Fragments arranged by size:
2288 161 104 102
PstI C_TGCA'G
Cuts at: 0 295 1301 2655
Size: 295 1006 1354
Fragments arranged by size:
1354 1006 295
PvuII CAG'CTG
Cuts at: 0 1473 2245 2655
Size: 1473 772 410
Fragments arranged by size:
1473 772 410
SacI G_AGCT'C
Cuts at: 0 926 2655
Size: 926 1729
SanDI GG'GwC_CC
Cuts at: 0 178 2655
Size: 178 2477
SfcI C'TryA_G
Cuts at: 0 291 1297 2478 2655
Size: 291 1006 1181 177
Fragments arranged by size:
1181 1006 291 177
SfoI GGC'GCC
Cuts at: 0 283 2655
Size: 283 2372
SmaI CCC'GGG
Cuts at: 0 1979 2655
Size: 1979 676
SmlI C'TyrA_G
Cuts at: 0 1129 1274 1536 2655
Size: 1129 145 262 1119
Fragments arranged by size:
1129 1119 262 145
SspI AAT'ATT
Cuts at: 0 480 2121 2655
Size: 480 1641 534
Fragments arranged by size:
1641 534 480
StuI AGG'CCT
Cuts at: 0 1520 2655
Size: 1520 1135
StyI C'CwwG_G
Cuts at: 0 1098 1319 1421 2363 2655
Size: 1098 221 102 942 292
Fragments arranged by size:
1098 942 292 221 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 154 1867 2655
Size: 154 1713 788
Fragments arranged by size:
1713 788 154
TatI w'GTAC_w
Cuts at: 0 1460 2655
Size: 1460 1195
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2432 2464 2655
Size: 2432 32 191
Fragments arranged by size:
2432 191 32
XbaI T'CTAG_A
Cuts at: 0 1434 2655
Size: 1434 1221
XhoI C'TCGA_G
Cuts at: 0 1274 2655
Size: 1274 1381
XmaI C'CCGG_G
Cuts at: 0 1977 2655
Size: 1977 678
XmnI GAAnn'nnTTC
Cuts at: 0 512 752 2290 2655
Size: 512 240 1538 365
Fragments arranged by size:
1538 512 365 240
ZraI GAC'GTC
Cuts at: 0 1866 2655
Size: 1866 789
Enzymes that do cut:
AarI AatII Acc65I AflIII AleI AlwNI ApaI ApaLI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BclI BfrBI BglII Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BstBI BstYI BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FspI FspAI HaeII Hin4I HincII KasI KpnI
MmeI MscI MslI MspA1I NarI NcoI NruI NsiI
NspI PciI PflMI PmlI PpuMI PsiI PspOMI PstI
PvuII SacI SanDI SfcI SfoI SmaI SmlI SspI
StuI StyI TaqII TatI TstI XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AccI AclI AcuI AfeI AflII AgeI AhdI AlfI
AloI ApoI AscI AseI AsiSI AvrII BaeI BciVI
BglI BlpI BmtI BsaI BsiEI BsiWI BsmBI BspEI
BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstZ17I
Bsu36I CspCI EagI EciI EcoRI FalI FseI HindIII
HpaI MfeI MluI NaeI NdeI NgoMIV NheI NotI
PacI PasI PfoI PmeI PpiI PshAI PspXI PsrI
PvuI RsrII SacII SalI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI
Tth111I XcmI