################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII AccI AccIII AclI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AvrII BamHI BbeI BbrPI BcgI BciVI BfrI BlnI BsaBI BsaWI BseAI BsiHKAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraII DraIII DrdI DsaI Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FseI HincII HindII HindIII HpaI KasI KspI MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### Acc65I 71 GGTACC G^GTACC AccII 166 CGCG CG^CG AcsI 946 GAATTC [GA]^AATT[CT] AcyI 771 GACGCC G[GA]^CG[CT]C ApoI 946 GAATTC [GA]^AATT[CT] Asp718I 71 GGTACC G^GTACC AspI 292 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 265 CCCGAG C^[CT]CG[GA]G AvaII 756 GGACC G^G[AT]CC BalI 467 TGGCCA TGG^CCA BanII 262 GAGCCC G[GA]GC[CT]^C BbsI 680 GTCTTC GAAGAC(2/6) BclI 641 TGATCA T^GATCA BcnI 807 CCCGG CC^[GC]GG BfaI 76 CTAG C^TAG BglI 194 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 451 AGATCT A^GATCT BmyI 262 GAGCCC G[AGT]GC[ACT]^C BpuAI 680 GTCTTC GAAGAC(2/6) BsaHI 771 GACGCC G[GA]^CG[CT]C BsgI 235 CTGCAC GTGCAG(16/14) BsiWI 908 CGTACG C^GTACG BsmFI 666 GGGAC GGGAC(10/14) BsoBI 265 CCCGAG C^[CT]CG[GA]G Bsp1286I 262 GAGCCC G[AGT]GC[ACT]^C BspMI 703 GCAGGT ACCTGC(4/8) BsrBI 403 GAGCGG CCGCTC(-3/-3) BsrDI 548 GCAATG GCAATG(2/0) BstUI 166 CGCG CG^CG BstYI 451 AGATCT [GA]^GATC[CT] EagI 226 CGGCCG C^GGCCG EarI 115 CTCTTC CTCTTC(1/4) EclXI 226 CGGCCG C^GGCCG Eco52I 226 CGGCCG C^GGCCG EcoRI 946 GAATTC G^AATTC FauI 135 GCGGG CCCGC(4/6) FbaI 641 TGATCA T^GATCA HaeII 121 AGCGCC [GA]GCGC^[CT] HgaI 771 GACGC GACGC(5/10) Hin1I 771 GACGCC G[GA]^CG[CT]C KpnI 71 GGTACC GGTAC^C Ksp632I 115 CTCTTC CTCTTC(1/4) MaeI 76 CTAG C^TAG MflI 451 AGATCT [GA]^GATC[CT] MluNI 467 TGGCCA TGG^CCA MscI 467 TGGCCA TGG^CCA MvnI 166 CGCG CG^CG NciI 807 CCCGG CC^[GC]GG NspI 185 ACATGC [GA]CATG^[CT] PflFI 292 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 612 CTGCAG CTGCA^G SapI 114 GCTCTTC GCTCTTC(1/4) SexAI 293 ACCAGGT A^CC[AT]GGT SfcI 612 CTGCAG C^T[GA][CT]AG SmlI 352 CTCAAG C^T[CT][GA]AG Tth111I 292 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 813 CCAGATGAAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 451 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AflIII 669 ACACGT A^C[GA][CT]GT 867 ACACGT AlwI 103 GATCC GGATC(4/5) 381 GGATC AviII 241 TGCGCA TGC^GCA 845 TGCGCA BanI 71 GGTACC G^G[CT][GA]CC 176 GGTGCC BlpI 189 GCTGAGC GC^T[ACGT]AGC 400 GCTGAGC Bpu1102I 189 GCTGAGC GC^T[ACGT]AGC 400 GCTGAGC BsaAI 670 CACGTA [CT]AC^GT[GA] 868 CACGTA BsaI 297 GGTCTC GGTCTC(1/5) 852 GGTCTC BseRI 268 GAGGAG GAGGAG(10/8) 441 GAGGAG BsiEI 222 CGACCG CG[GA][CT]^CG 226 CGGCCG BsrFI 68 GCCGGT [GA]^CCGG[CT] 224 ACCGGC BstF5I 282 CATCC GGATG(2/0) 385 CATCC Cac8I 141 GCTGGC GC[ACGT]^[ACGT]GC 260 GCGAGC CelII 189 GCTGAGC GC^T[ACGT]AGC 400 GCTGAGC Cfr10I 68 GCCGGT [GA]^CCGG[CT] 224 ACCGGC EaeI 226 CGGCCG [CT]^GGCC[GA] 467 TGGCCA FokI 282 CATCC GGATG(9/13) 385 CATCC FspI 241 TGCGCA TGC^GCA 845 TGCGCA MaeII 671 ACGT A^CGT 869 ACGT MseI 514 TTAA T^TAA 688 TTAA MslI 670 CACGTAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 837 CACTGTGGTG PflMI 459 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 496 CCAGGAAATGG PleI 564 GACTC GAGTC(4/5) 485 GAGTC SfaNI 446 GCATC GCATC(5/9) 603 GCATC TaqI 305 TCGA T^CGA 606 TCGA TfiI 348 GATTC G^A[AT]TC 506 GATTC Tru9I 514 TTAA T^TAA 688 TTAA Tsp509I 627 AATT ^AATT 947 AATT TthHB8I 305 TCGA T^CGA 606 TCGA Van91I 459 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 496 CCAGGAAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AfaI AluI BbvCI BbvI BpmI BsaJI BsiYI BslI BsmAI BsrI BssKI BstNI CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI TseI Tsp45I TspRI