Restriction map for
pFN21AE4311
(Circular) (Six-base) MAPSORT of: pFN21AE4311.seq Check: 7880 from: 1 to: 5671
pFN21AE4311 5671 bp
With 182 enzymes: SgfI *
March 28, 2012 14:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3481 278
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3014 1937
Size: 1077 4594
Acc65I G'GTAC_C
Cuts at: 2076 2992 2076
Size: 916 4755
AclI AA'CG_TT
Cuts at: 1563 3798 4171 1563
Size: 2235 373 3063
Fragments arranged by size:
3063 2235 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2009 2107 2156 2354 2672 3734 5118 5386
Size: 377 98 49 198 318 1062 1384 268
Cuts at: 5386 1632
Size: 1917
Fragments arranged by size:
1917 1384 1062 377 318 268 198 98
49
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5474
AflIII A'CryG_T
Cuts at: 1203 2674 2872 4570 5547 1203
Size: 1471 198 1698 977 1327
Fragments arranged by size:
1698 1471 1327 977 198
AgeI A'CCGG_T
Cuts at: 4502 4502
Size: 5671
AhdI GACnn_n'nnGTC
Cuts at: 4400 4400
Size: 5671
AleI CACnn'nnGTG
Cuts at: 1166 2846 1166
Size: 1680 3991
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5671
AlwNI CAG_nnn'CTG
Cuts at: 3414 4986 3414
Size: 1572 4099
ApaLI G'TGCA_C
Cuts at: 3728 4884 3728
Size: 1156 4515
ApoI r'AATT_y
Cuts at: 1493 2951 2980 3206 3358 5467 1493
Size: 1458 29 226 152 2109 1697
Fragments arranged by size:
2109 1697 1458 226 152 29
AseI AT'TA_AT
Cuts at: 160 1032 4225 160
Size: 872 3193 1606
Fragments arranged by size:
3193 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
AvaI C'yCGr_G
Cuts at: 1870 1943 2270 2996 1870
Size: 73 327 726 4545
Fragments arranged by size:
4545 726 327 73
BamHI G'GATC_C
Cuts at: 3001 3368 3001
Size: 367 5304
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2076 2181 2992 4447 618
Size: 325 217 627 289 105 811 1455 1842
Fragments arranged by size:
1842 1455 811 627 325 289 217 105
BanII G_rGCy'C
Cuts at: 1391 2271 2990 1391
Size: 880 719 4072
Fragments arranged by size:
4072 880 719
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2678 928
Size: 971 779 3921
Fragments arranged by size:
3921 971 779
BbvCI CC'TCA_GC
Cuts at: 2087 2401 2704 2087
Size: 314 303 5054
Fragments arranged by size:
5054 314 303
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3862 3896 1903
Size: 34 1925 34 3678
Fragments arranged by size:
3678 1925 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3563 4773 3563
Size: 1210 4461
BclI T'GATC_A
Cuts at: 1548 2646 1548
Size: 1098 4573
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2205 4282 136
Size: 107 122 71 631 605 533 2077 1525
Fragments arranged by size:
2077 1525 631 605 533 122 107 71
BglII A'GATC_T
Cuts at: 2456 5666 2456
Size: 3210 2461
BlpI GC'TnA_GC
Cuts at: 2195 2406 3088 2195
Size: 211 682 4778
Fragments arranged by size:
4778 682 211
Bme1580I G_kGCm'C
Cuts at: 1790 3732 4888 1790
Size: 1942 1156 2573
Fragments arranged by size:
2573 1942 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5671
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2370 3402 4360
Size: 416 53 83 399 24 953 1032 958
Cuts at: 4360 442
Size: 1753
Fragments arranged by size:
1753 1032 958 953 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2186 2845 2927 4331 1239
Size: 947 659 82 1404 2579
Fragments arranged by size:
2579 1404 947 659 82
Bpu10I CC'TnA_GC
Cuts at: 2087 2401 2704 2087
Size: 314 303 5054
Fragments arranged by size:
5054 314 303
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2342 3796 4661 4959 2342
Size: 1454 865 298 3054
Fragments arranged by size:
3054 1454 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2308 2863 4334 882
Size: 617 809 555 1471 2219
Fragments arranged by size:
2219 1471 809 617 555
BsaAI yAC'GTr
Cuts at: 493 1204 2677 2875 3462 493
Size: 711 1473 198 587 2702
Fragments arranged by size:
2702 1473 711 587 198
BsaBI GATnn'nnATC
Cuts at: 5665 5665
Size: 5671
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2777 3478 3860 4495
Size: 53 83 186 936 1244 701 382 635
Cuts at: 4495 275
Size: 1451
Fragments arranged by size:
1451 1244 936 701 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4104 4502 4776 4923 5334 4104
Size: 398 274 147 411 4441
Fragments arranged by size:
4441 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2147 2177 2288 2318 2718
Size: 30 165 30 744 30 111 30 400
Cuts at: 2718 2748 1178
Size: 30 4101
Fragments arranged by size:
4101 744 400 165 111 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2288 2461 1186
Size: 1102 173 4396
Fragments arranged by size:
4396 1102 173
BseYI C'CCAG_C
Cuts at: 2150 2743 4874 5370 2150
Size: 593 2131 496 2451
Fragments arranged by size:
2451 2131 593 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2225 1163
Size: 573 489 4609
Fragments arranged by size:
4609 573 489
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2230 2234 3121 3882 4910 1540
Size: 275 186 229 4 887 761 1028 2301
Fragments arranged by size:
2301 1028 887 761 275 229 186 4
BsiHKAI G_wGCw'C
Cuts at: 3732 3817 4888 3732
Size: 85 1071 4515
Fragments arranged by size:
4515 1071 85
BsiWI C'GTAC_G
Cuts at: 2913 2913
Size: 5671
BsmI GAATG_Cn'
Cuts at: 3182 3275 3182
Size: 93 5578
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2271 2990 3732 3817 4888 1391
Size: 399 481 719 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 719 481 399 85
BspEI T'CCGG_A
Cuts at: 5334 5334
Size: 5671
BspHI T'CATG_A
Cuts at: 3560 5290 3560
Size: 1730 3941
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2699 3026 844
Size: 1855 327 3489
Fragments arranged by size:
3489 1855 327
BsrBI CCG'CTC
Cuts at: 1394 1823 2410 3558 1394
Size: 429 587 1148 3507
Fragments arranged by size:
3507 1148 587 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2560 4166 4340 65
Size: 1183 1312 1606 174 1396
Fragments arranged by size:
1606 1396 1312 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2073 2229 4315 4502 1700
Size: 110 263 156 2086 187 2869
Fragments arranged by size:
2869 2086 263 187 156 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5671
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5671
BssSI C'ACGA_G
Cuts at: 3731 4743 3731
Size: 1012 4659
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5671
BstBI TT'CG_AA
Cuts at: 4475 4475
Size: 5671
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5671
BstYI r'GATC_y
Cuts at: 1972 2456 3001 3368 3753 3770 5222 5308 5320
Size: 484 545 367 385 17 1452 86 12
Cuts at: 5320 5666 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 545 484 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5119
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4932
Fragments arranged by size:
4932 683 56
BtsI GCAGTG_nn'
Cuts at: 2242 3187 3981 4001 2242
Size: 945 794 20 3912
Fragments arranged by size:
3912 945 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5671
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5636
DraI TTT'AAA
Cuts at: 2975 2975
Size: 5671
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4678 809
Size: 507 3362 1802
Fragments arranged by size:
3362 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2231 2472 3118
Size: 54 1425 50 275 18 401 241 646
Cuts at: 3118 4007 5636 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 646 401 275 241 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2231 3118 1537
Size: 275 419 887 4090
Fragments arranged by size:
4090 887 419 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2126 3601 1353
Size: 773 1475 3423
Fragments arranged by size:
3423 1475 773
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2114 4258 4632 4778 387
Size: 1064 138 525 2144 374 146 1280
Fragments arranged by size:
2144 1280 1064 525 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2009 2107 2156 2186 2354 2672 2845
Size: 393 377 98 49 30 168 318 173
Cuts at: 2845 2927 3734 4331 5118 5386 1239
Size: 82 807 597 787 268 1524
Fragments arranged by size:
1524 807 787 597 393 377 318 268
173 168 98 82 49 30
EcoRI G'AATT_C
Cuts at: 2951 2980 3358 2951
Size: 29 378 5264
Fragments arranged by size:
5264 378 29
FspI TGC'GCA
Cuts at: 2248 2852 4177 2248
Size: 604 1325 3742
Fragments arranged by size:
3742 1325 604
FspAI rTGC'GCAy
Cuts at: 2852 2852
Size: 5671
HaeII r_GCGC'y
Cuts at: 1678 2130 4818 1678
Size: 452 2688 2531
Fragments arranged by size:
2688 2531 452
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2288 2320 4312 4344 4386
Size: 32 135 32 716 32 1992 32 42
Cuts at: 4386 4418 1373
Size: 32 2626
Fragments arranged by size:
2626 1992 716 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3015 3261 669
Size: 988 281 1077 246 3079
Fragments arranged by size:
3079 1077 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5671
HpaI GTT'AAC
Cuts at: 3261 3261
Size: 5671
KpnI G_GTAC'C
Cuts at: 2080 2996 2080
Size: 916 4755
MfeI C'AATT_G
Cuts at: 3270 3270
Size: 5671
MluI A'CGCG_T
Cuts at: 5547 5547
Size: 5671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2330 4785 4969 1305
Size: 1025 2455 184 2007
Fragments arranged by size:
2455 2007 1025 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2474 10
Size: 54 1425 343 642 3207
Fragments arranged by size:
3207 1425 642 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2679 2846 3629 3988 4147 518
Size: 648 1513 167 783 359 159 2042
Fragments arranged by size:
2042 1513 783 648 359 167 159
MspA1I CmG'CkG
Cuts at: 1606 2239 2405 2905 3087 3764 4912 5157 1606
Size: 633 166 500 182 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 633 500 245 182 166
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5119
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5671
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5671
NotI GC'GGCC_GC
Cuts at: 3118 3118
Size: 5671
NspI r_CATG'y
Cuts at: 1132 1229 2194 3029 4574 1132
Size: 97 965 835 1545 2229
Fragments arranged by size:
2229 1545 965 835 97
PasI CC'CwG_GG
Cuts at: 1348 2668 1348
Size: 1320 4351
PciI A'CATG_T
Cuts at: 4570 4570
Size: 5671
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2470 2507 1108
Size: 1362 37 4272
Fragments arranged by size:
4272 1362 37
PmeI GTTT'AAAC
Cuts at: 2975 2975
Size: 5671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3740 3772 5253 5285 3740
Size: 32 1481 32 4126
Fragments arranged by size:
4126 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5671
PsiI TTA'TAA
Cuts at: 3241 3241
Size: 5671
PstI C_TGCA'G
Cuts at: 830 2621 3023 830
Size: 1791 402 3478
Fragments arranged by size:
3478 1791 402
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5671
PvuII CAG'CTG
Cuts at: 2239 2405 2905 2239
Size: 166 500 5005
Fragments arranged by size:
5005 500 166
SalI G'TCGA_C
Cuts at: 1936 3013 1936
Size: 1077 4594
SapI GCTCTTCn'nnn_
Cuts at: 2126 2126
Size: 5671
SbfI CC_TGCA'GG
Cuts at: 3023 3023
Size: 5671
ScaI AGT'ACT
Cuts at: 3919 5631 3919
Size: 1712 3959
SexAI A'CCwGG_T
Cuts at: 2298 2298
Size: 5671
SfcI C'TryA_G
Cuts at: 826 1045 2617 3019 4154 4835 5026 826
Size: 219 1572 402 1135 681 191 1471
Fragments arranged by size:
1572 1471 1135 681 402 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5671
SmaI CCC'GGG
Cuts at: 1872 2998 1872
Size: 1126 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2357 3775 4676 4938 820
Size: 197 926 414 1418 901 262 1553
Fragments arranged by size:
1553 1418 926 901 414 262 197
SnaBI TAC'GTA
Cuts at: 493 3462 493
Size: 2969 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5671
SphI G_CATG'C
Cuts at: 1132 3029 1132
Size: 1897 3774
SspI AAT'ATT
Cuts at: 5 52 3595 5
Size: 47 3543 2081
Fragments arranged by size:
3543 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5671
StyI C'CwwG_G
Cuts at: 513 1065 3110 513
Size: 552 2045 3074
Fragments arranged by size:
3074 2045 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3698 3715 3868 4053 4498 325
Size: 1255 2118 17 153 185 445 1498
Fragments arranged by size:
2118 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3917 5629 96
Size: 274 80 33 51 1443 1940 1712 138
Fragments arranged by size:
1940 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2718 2750 5631 5663 2718
Size: 32 2881 32 2726
Fragments arranged by size:
2881 2726 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2300 1316
Size: 984 4687
XbaI T'CTAG_A
Cuts at: 3007 3007
Size: 5671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2825 2825
Size: 5671
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5671
XmaI C'CCGG_G
Cuts at: 1870 2996 1870
Size: 1126 4545
XmnI GAAnn'nnTTC
Cuts at: 3800 3800
Size: 5671
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3479 276
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI
SrfI SwaI