################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AfaI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BpmI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MroI MslI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AcsI 852 GAATTC [GA]^AATT[CT] AcyI 85 GGCGCC G[GA]^CG[CT]C AflIII 48 ACACGT A^C[GA][CT]GT ApaI 499 GGGCCC GGGCC^C ApoI 852 GAATTC [GA]^AATT[CT] AspHI 172 GTGCTC G[AT]GC[AT]^C AvaI 236 CCCGGG C^[CT]CG[GA]G BalI 573 TGGCCA TGG^CCA BanI 85 GGCGCC G^G[CT][GA]CC BbeI 85 GGCGCC GGCGC^C BbsI 79 GTCTTC GAAGAC(2/6) BfaI 905 CTAG C^TAG BlpI 19 GCTGAGC GC^T[ACGT]AGC Bpu1102I 19 GCTGAGC GC^T[ACGT]AGC BpuAI 79 GTCTTC GAAGAC(2/6) BsaHI 85 GGCGCC G[GA]^CG[CT]C BsiHKAI 172 GTGCTC G[AT]GC[AT]^C BsmI 567 GAATGC GAATGC(1/-1) BsoBI 236 CCCGGG C^[CT]CG[GA]G BsrBI 231 CCGCTC CCGCTC(-3/-3) BstAPI 10 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 605 AGATCC [GA]^GATC[CT] CelII 19 GCTGAGC GC^T[ACGT]AGC DrdI 43 GACAGACACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 573 TGGCCA [CT]^GGCC[GA] EcoRI 852 GAATTC G^AATTC EcoT14I 167 CCATGG C^C[AT][AT]GG HaeII 85 GGCGCC [GA]GCGC^[CT] HgaI 380 GCGTC GACGC(5/10) Hin1I 85 GGCGCC G[GA]^CG[CT]C HphI 640 GGTGA GGTGA(8/7) KasI 85 GGCGCC G^GCGCC MaeI 905 CTAG C^TAG MaeII 50 ACGT A^CGT MflI 605 AGATCC [GA]^GATC[CT] MluNI 573 TGGCCA TGG^CCA MscI 573 TGGCCA TGG^CCA MseI 548 TTAA T^TAA MspA1I 11 CAGCTG C[AC]G^C[GT]G NarI 85 GGCGCC GG^CGCC NcoI 167 CCATGG C^CATGG PflMI 609 CCAGATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 11 CAGCTG CAG^CTG SfaNI 580 GATGC GCATC(5/9) SfoI 85 GGCGCC GGC^GCC SmaI 236 CCCGGG CCC^GGG SmlI 175 CTCAAG C^T[CT][GA]AG SspI 654 AATATT AAT^ATT StuI 866 AGGCCT AGG^CCT StyI 167 CCATGG C^C[AT][AT]GG Tru9I 548 TTAA T^TAA Van91I 609 CCAGATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 605 AGATCC [GA]^GATC[CT] XmaCI 236 CCCGGG C^CCGGG XmaI 236 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AlwI 222 GATCC GGATC(4/5) 606 GATCC BanII 460 GAGCCC G[GA]GC[CT]^C 499 GGGCCC BsgI 257 GTGCAG GTGCAG(16/14) 803 GTGCAG BsiEI 291 CGACCG CG[GA][CT]^CG 704 CGGTCG DsaI 167 CCATGG C^C[GA][CT]GG 200 CCGTGG EarI 552 GAAGAG CTCTTC(1/4) 595 GAAGAG FauI 230 CCCGC CCCGC(4/6) 184 GCGGG HapII 237 CCGG C^CGG 465 CCGG HpaII 237 CCGG C^CGG 465 CCGG Ksp632I 552 GAAGAG CTCTTC(1/4) 595 GAAGAG MaeIII 631 GTCAC ^GT[ACGT]AC 641 GTGAC MspI 237 CCGG C^CGG 465 CCGG PpuMI 395 GGGTCCC [GA]G^G[AT]CC[CT] 784 AGGACCC SphI 136 GCATGC GCATG^C 368 GCATGC TfiI 688 GAATC G^A[AT]TC 826 GAATC Tsp45I 631 GTCAC ^GT[GC]AC 641 GTGAC Tsp509I 853 AATT ^AATT 896 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AluI AlwNI AvaII BbvI BcnI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HinfI ItaI MboI MboII MnlI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI Sau3AI Sau96I ScrFI SfcI TseI TspRI