Restriction map for
pFN21AE3018
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwNI
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AvaII AviII AvrII BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BsrI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
Cac8I CelII Cfr13I ClaI CpoI
DdeI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HincII HindII HindIII HinfI
HpaI Ksp632I KspI MaeI MamI
MfeI MflI MluI MroI MseI
MslI MspA1I MunI NaeI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI SapI
Sau96I SbfI ScaI SexAI SfcI
SfiI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tru9I Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 70 GGTACC G^GTACC
AccII 13 CGCG CG^CG
AfaI 71 GTAC GT^AC
AflIII 201 ACGTGT A^C[GA][CT]GT
AlwI 284 GGATC GGATC(4/5)
Aor51HI 165 AGCGCT AGC^GCT
Asp718I 70 GGTACC G^GTACC
BalI 228 TGGCCA TGG^CCA
BbeI 37 GGCGCC GGCGC^C
BpmI 97 CTCCAG CTGGAG(16/14)
BsgI 263 GTGCAG GTGCAG(16/14)
BsrFI 250 GCCGGT [GA]^CCGG[CT]
BssSI 145 CACGAG CACGAG(-5/-1)
BstF5I 132 GGATG GGATG(2/0)
BstUI 13 CGCG CG^CG
Cfr10I 250 GCCGGT [GA]^CCGG[CT]
DsaI 128 CCGTGG C^C[GA][CT]GG
EaeI 228 TGGCCA [CT]^GGCC[GA]
Eco47III 165 AGCGCT AGC^GCT
FauI 11 CCCGC CCCGC(4/6)
FokI 132 GGATG GGATG(9/13)
HaeIII 229 GGCC GG^CC
HapII 251 CCGG C^CGG
HpaII 251 CCGG C^CGG
HphI 253 GGTGA GGTGA(8/7)
KasI 37 GGCGCC G^GCGCC
KpnI 70 GGTACC GGTAC^C
MaeII 201 ACGT A^CGT
MluNI 228 TGGCCA TGG^CCA
MscI 228 TGGCCA TGG^CCA
MspI 251 CCGG C^CGG
MvnI 13 CGCG CG^CG
MwoI 48 GCTGCTGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 37 GGCGCC GG^CGCC
NlaIII 240 CATG CATG^
RsaI 71 GTAC GT^AC
SfoI 37 GGCGCC GGC^GCC
SgrAI 249 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 190 CTCAAG C^T[CT][GA]AG
TaqI 161 TCGA T^CGA
Tsp509I 325 AATT ^AATT
TthHB8I 161 TCGA T^CGA
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# TWO CUT ENZYMES ( 22 enzymes) #
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AciI 12 CCGC CCGC(-3/-1)
41 CCGC
AspHI 6 GAGCTC G[AT]GC[AT]^C
94 GAGCTC
AvaI 104 CCCGAG C^[CT]CG[GA]G
308 CCCGAG
BanI 37 GGCGCC G^G[CT][GA]CC
70 GGTACC
BsiHKAI 6 GAGCTC G[AT]GC[AT]^C
94 GAGCTC
BsmFI 235 GTCCC GGGAC(10/14)
305 GTCCC
BsoBI 104 CCCGAG C^[CT]CG[GA]G
308 CCCGAG
CfoI 38 GCGC GCG^C
166 GCGC
DpnII 159 GATC ^GATC
285 GATC
HaeII 37 GGCGCC [GA]GCGC^[CT]
165 AGCGCT
HgaI 88 GACGC GACGC(5/10)
247 GACGC
HhaI 38 GCGC GCG^C
166 GCGC
HinP1I 38 GCGC G^CGC
166 GCGC
MaeIII 245 GTGAC ^GT[ACGT]AC
299 GTGAC
MboI 159 GATC ^GATC
285 GATC
MboII 183 GAAGA GAAGA(8/7)
219 GAAGA
NdeII 159 GATC ^GATC
285 GATC
SacI 6 GAGCTC GAGCT^C
94 GAGCTC
Sau3AI 159 GATC ^GATC
285 GATC
SfaNI 54 GATGC GCATC(5/9)
136 GATGC
Tsp45I 245 GTGAC ^GT[GC]AC
299 GTGAC
TspRI 259 CAGTG CA[GC]TG[ACGT][ACGT]^
266 CAGTG
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AcyI AluI BanII BbvI BmyI
BsaHI BsaJI BsiYI BslI Bsp1286I
BssKI BstNI EcoRII Fnu4HI Hin1I
ItaI MnlI MvaI NlaIV ScrFI
TseI