################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI AluI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BalI BanII BbeI BbrPI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccII 122 CGCG CG^CG AcsI 391 AAATTC [GA]^AATT[CT] ApoI 391 AAATTC [GA]^AATT[CT] AspHI 139 GTGCTC G[AT]GC[AT]^C AvaI 234 CCCGGG C^[CT]CG[GA]G BamHI 267 GGATCC G^GATCC BanI 313 GGCACC G^G[CT][GA]CC BbsI 423 GTCTTC GAAGAC(2/6) BbvCI 240 GCTGAGG CCTCAGC(-5/-2) BglI 350 GCCAGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 139 GTGCTC G[AGT]GC[ACT]^C BpuAI 423 GTCTTC GAAGAC(2/6) BseRI 28 CTCCTC GAGGAG(10/8) BsgI 38 CTGCAC GTGCAG(16/14) BsiHKAI 139 GTGCTC G[AT]GC[AT]^C BsmI 507 GAATGC GAATGC(1/-1) BsoBI 234 CCCGGG C^[CT]CG[GA]G Bsp1286I 139 GTGCTC G[AGT]GC[ACT]^C BspMI 340 ACCTGC ACCTGC(4/8) BsrI 351 CCAGT ACTGG(1/-1) BssSI 31 CTCGTG CACGAG(-5/-1) BstAPI 101 GCAGTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 122 CGCG CG^CG BstYI 267 GGATCC [GA]^GATC[CT] Cfr13I 232 GGCCC G^G[ACGT]CC DpnII 268 GATC ^GATC DraIII 43 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 50 TGGCCG [CT]^GGCC[GA] HgaI 8 GCGTC GACGC(5/10) HphI 277 GGTGA GGTGA(8/7) MboI 268 GATC ^GATC MflI 267 GGATCC [GA]^GATC[CT] MseI 541 TTAA T^TAA MvnI 122 CGCG CG^CG NdeII 268 GATC ^GATC PstI 173 CTGCAG CTGCA^G SapI 3 GAAGAGC GCTCTTC(1/4) Sau3AI 268 GATC ^GATC Sau96I 232 GGCCC G^G[ACGT]CC SexAI 44 ACCTGGT A^CC[AT]GGT SfcI 173 CTGCAG C^T[GA][CT]AG SmaI 234 CCCGGG CCC^GGG TaqI 143 TCGA T^CGA Tru9I 541 TTAA T^TAA TthHB8I 143 TCGA T^CGA XhoII 267 GGATCC [GA]^GATC[CT] XmaCI 234 CCCGGG C^CCGGG XmaI 234 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AlwI 268 GATCC GGATC(4/5) 267 GGATC BpmI 57 CTGGAG CTGGAG(16/14) 467 CTGGAG BsaI 337 GAGACC GGTCTC(1/5) 518 GGTCTC BsaJI 234 CCCGGG C^C[ACGT][ACGT]GG 235 CCGGGG BsiYI 253 CCTTCTAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 524 CCGTAATGAGG BslI 253 CCTTCTAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 524 CCGTAATGAGG BsmFI 373 GGGAC GGGAC(10/14) 305 GTCCC BstF5I 216 GGATG GGATG(2/0) 323 GGATG BstNI 45 CCTGG CC^[AT]GG 56 CCTGG Cac8I 36 GCCTGC GC[ACGT]^[ACGT]GC 119 GCCCGC CfoI 123 GCGC GCG^C 170 GCGC DdeI 207 CTCAG C^T[ACGT]AG 241 CTGAG EarI 3 GAAGAG CTCTTC(1/4) 280 GAAGAG EcoRII 45 CCTGG ^CC[AT]GG 56 CCTGG FauI 120 CCCGC CCCGC(4/6) 180 GCGGG FokI 216 GGATG GGATG(9/13) 323 GGATG HaeIII 51 GGCC GG^CC 232 GGCC HapII 235 CCGG C^CGG 515 CCGG HhaI 123 GCGC GCG^C 170 GCGC HinP1I 123 GCGC G^CGC 170 GCGC HpaII 235 CCGG C^CGG 515 CCGG Ksp632I 3 GAAGAG CTCTTC(1/4) 280 GAAGAG MspI 235 CCGG C^CGG 515 CCGG MvaI 45 CCTGG CC^[AT]GG 56 CCTGG NlaIV 267 GGATCC GG[ACGT]^[ACGT]CC 313 GGCACC SfaNI 359 GCATC GCATC(5/9) 463 GCATC SspI 407 AATATT AAT^ATT 478 AATATT Tsp45I 95 GTGAC ^GT[GC]AC 365 GTGAC Tsp509I 67 AATT ^AATT 392 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### AciI BbvI BcnI BsmAI BssKI Fnu4HI ItaI MaeIII MboII MnlI MwoI NciI NlaIII ScrFI TseI TspRI