(Linear) (Six-base) MAPSORT of: KIEE1665.seq  Check: 7269  from: 1  to: 552



KIEE1665.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:44  ..

ApoI r'AATT_y

Cuts at:      0     391     552 
   Size:        391     161
 
AvaI C'yCGr_G

Cuts at:      0     234     552 
   Size:        234     318
 
BamHI G'GATC_C

Cuts at:      0     267     552 
   Size:        267     285
 
BanI G'GyrC_C

Cuts at:      0     313     552 
   Size:        313     239
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     416     552 
   Size:        416     136
 
BbvCI CC'TCA_GC

Cuts at:      0     241     552 
   Size:        241     311
 
BglI GCCn_nnn'nGGC

Cuts at:      0     356     552 
   Size:        356     196
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      78     488     552 
   Size:         78     410      64

  Fragments arranged by size: 

                 410      78      64
 
Bpu10I CC'TnA_GC

Cuts at:      0     241     552 
   Size:        241     311
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     331     524     552 
   Size:        331     193      28

  Fragments arranged by size: 

                 331     193      28
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19     552 
   Size:         19     533
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      23     552 
   Size:         23     529
 
BsiHKAI G_wGCw'C

Cuts at:      0     143     552 
   Size:        143     409
 
BsmI GAATG_Cn'

Cuts at:      0     513     552 
   Size:        513      39
 
Bsp1286I G_dGCh'C

Cuts at:      0     143     552 
   Size:        143     409
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     349     552 
   Size:        349     203
 
BssSI C'ACGA_G

Cuts at:      0      31     552 
   Size:         31     521
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     107     552 
   Size:        107     445
 
BstYI r'GATC_y

Cuts at:      0     267     552 
   Size:        267     285
 
BtsI GCAGTG_nn'

Cuts at:      0     108     552 
   Size:        108     444
 
DraIII CAC_nnn'GTG

Cuts at:      0      48     552 
   Size:         48     504
 
EaeI y'GGCC_r

Cuts at:      0      50     552 
   Size:         50     502
 
EarI CTCTTCn'nnn_

Cuts at:      0     275     552 
   Size:        275     277
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      78     488     552 
   Size:         78     410      64

  Fragments arranged by size: 

                 410      78      64
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      27     552 
   Size:         27     525
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     448     480     552 
   Size:        448      32      72

  Fragments arranged by size: 

                 448      72      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     452     552 
   Size:        452     100
 
PstI C_TGCA'G

Cuts at:      0     177     552 
   Size:        177     375
 
SexAI A'CCwGG_T

Cuts at:      0      44     552 
   Size:         44     508
 
SfcI C'TryA_G

Cuts at:      0     173     552 
   Size:        173     379
 
SmaI CCC'GGG

Cuts at:      0     236     552 
   Size:        236     316
 
SspI AAT'ATT

Cuts at:      0     409     480     552 
   Size:        409      71      72

  Fragments arranged by size: 

                 409      72      71
 
XmaI C'CCGG_G

Cuts at:      0     234     552 
   Size:        234     318
 

 Enzymes that do cut:

     ApoI     AvaI    BamHI     BanI     BbsI    BbvCI     BglI     BpmI
   Bpu10I     BsaI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI
    BssSI   BstAPI    BstYI     BtsI   DraIII     EaeI     EarI  Eco57MI
     FalI    Hin4I     MmeI     PstI    SexAI     SfcI     SmaI     SspI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BanII     BbeI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI     DrdI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmnI     ZraI