################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HincII HindII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MseI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tsp45I Van91I XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AciI 175 GCGG CCGC(-3/-1) AcsI 410 GAATTT [GA]^AATT[CT] AcyI 183 GACGCC G[GA]^CG[CT]C AfaI 351 GTAC GT^AC AlwNI 214 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 132 CCTGAGG CC^T[ACGT]AGG ApoI 410 GAATTT [GA]^AATT[CT] AspHI 316 GAGCAC G[AT]GC[AT]^C AspI 226 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 432 GGATCC G^GATCC BanI 20 GGCACC G^G[CT][GA]CC BbvI 148 GCAGC GCAGC(8/12) BfaI 73 CTAG C^TAG BmyI 316 GAGCAC G[AGT]GC[ACT]^C BpmI 161 CTCCAG CTGGAG(16/14) BsaHI 183 GACGCC G[GA]^CG[CT]C BseRI 158 CTCCTC GAGGAG(10/8) BsiHKAI 316 GAGCAC G[AT]GC[AT]^C BsiYI 190 CCCCGAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 190 CCCCGAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmBI 181 GAGACG CGTCTC(1/5) BsmFI 442 GGGAC GGGAC(10/14) Bsp1286I 316 GAGCAC G[AGT]GC[ACT]^C BspHI 402 TCATGA T^CATGA BstBI 268 TTCGAA TT^CGAA BstF5I 312 GGATG GGATG(2/0) Bsu36I 132 CCTGAGG CC^T[ACGT]AGG CfoI 151 GCGC GCG^C EarI 52 GAAGAG CTCTTC(1/4) Eco81I 132 CCTGAGG CC^T[ACGT]AGG FokI 312 GGATG GGATG(9/13) HaeIII 116 GGCC GG^CC HgaI 183 GACGC GACGC(5/10) HhaI 151 GCGC GCG^C Hin1I 183 GACGCC G[GA]^CG[CT]C HinP1I 151 GCGC G^CGC HindIII 336 AAGCTT A^AGCTT HphI 341 TCACC GGTGA(8/7) Ksp632I 52 GAAGAG CTCTTC(1/4) MaeI 73 CTAG C^TAG MaeII 227 ACGT A^CGT MaeIII 44 GTTAC ^GT[ACGT]AC MspA1I 173 CAGCGG C[AC]G^C[GT]G MwoI 97 GCTTTATATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NspV 268 TTCGAA TT^CGAA PaeR7I 65 CTCGAG C^TCGAG PflFI 226 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 169 GACTC GAGTC(4/5) RcaI 402 TCATGA T^CATGA RsaI 351 GTAC GT^AC SfuI 268 TTCGAA TT^CGAA TfiI 33 GATTC G^A[AT]TC TseI 148 GCAGC G^C[AT]GC Tsp509I 411 AATT ^AATT Tth111I 226 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 72 TCTAGA T^CTAGA XhoI 65 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AluI 63 AGCT AG^CT 337 AGCT AvaI 65 CTCGAG C^[CT]CG[GA]G 191 CCCGAG AvaII 129 GGACC G^G[AT]CC 231 GGTCC BbsI 76 GAAGAC GAAGAC(2/6) 247 GAAGAC BpuAI 76 GAAGAC GAAGAC(2/6) 247 GAAGAC BsaJI 112 CCTGGG C^C[ACGT][ACGT]GG 143 CCAGGG BsmAI 181 GAGAC GTCTC(1/5) 156 GTCTC BsoBI 65 CTCGAG C^[CT]CG[GA]G 191 CCCGAG BstYI 215 AGATCC [GA]^GATC[CT] 432 GGATCC Cac8I 83 GCAGGC GC[ACGT]^[ACGT]GC 275 GCTGGC Fnu4HI 148 GCAGC GC^[ACGT]GC 175 GCGGC HinfI 33 GATTC G^A[ACGT]TC 169 GACTC ItaI 148 GCAGC GC^[ACGT]GC 175 GCGGC MflI 215 AGATCC [GA]^GATC[CT] 432 GGATCC NlaIII 94 CATG CATG^ 403 CATG PstI 81 CTGCAG CTGCA^G 389 CTGCAG SfcI 81 CTGCAG C^T[GA][CT]AG 389 CTGCAG SmlI 65 CTCGAG C^T[CT][GA]AG 330 CTCAAG TaqI 66 TCGA T^CGA 269 TCGA TthHB8I 66 TCGA T^CGA 269 TCGA XhoII 215 AGATCC [GA]^GATC[CT] 432 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AlwI BsrI BssKI BstNI Cfr13I DdeI DpnII EcoRII MboI MboII MnlI MvaI NdeII NlaIV Sau3AI Sau96I ScrFI TspRI