(Linear) (Six-base) MAPSORT of: KIEE6526.seq  Check: 1443  from: 1  to: 450



KIEE6526.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     266     450 
   Size:        266     184
 
AlwNI CAG_nnn'CTG

Cuts at:      0     219     450 
   Size:        219     231
 
ApoI r'AATT_y

Cuts at:      0     410     450 
   Size:        410      40
 
AvaI C'yCGr_G

Cuts at:      0      65     191     450 
   Size:         65     126     259

  Fragments arranged by size: 

                 259     126      65
 
BamHI G'GATC_C

Cuts at:      0     432     450 
   Size:        432      18
 
BanI G'GyrC_C

Cuts at:      0      20     450 
   Size:         20     430
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      83     254     450 
   Size:         83     171     196

  Fragments arranged by size: 

                 196     171      83
 
BmgBI CAC'GTC

Cuts at:      0     228     450 
   Size:        228     222
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     146     450 
   Size:        146     304
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     315     450 
   Size:        315     135
 
BsaHI Gr'CG_yC

Cuts at:      0     184     450 
   Size:        184     266
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     149     450 
   Size:        149     301
 
BsiHKAI G_wGCw'C

Cuts at:      0     320     450 
   Size:        320     130
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     175     450 
   Size:        175     275
 
Bsp1286I G_dGCh'C

Cuts at:      0     320     450 
   Size:        320     130
 
BspHI T'CATG_A

Cuts at:      0     402     450 
   Size:        402      48
 
BstBI TT'CG_AA

Cuts at:      0     269     450 
   Size:        269     181
 
BstYI r'GATC_y

Cuts at:      0     215     432     450 
   Size:        215     217      18

  Fragments arranged by size: 

                 217     215      18
 
Bsu36I CC'TnA_GG

Cuts at:      0     133     450 
   Size:        133     317
 
BtsI GCAGTG_nn'

Cuts at:      0     106     450 
   Size:        106     344
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     107     142     450 
   Size:        107      35     308

  Fragments arranged by size: 

                 308     107      35
 
EarI CTCTTCn'nnn_

Cuts at:      0      47     450 
   Size:         47     403
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     146     266     450 
   Size:        146     120     184

  Fragments arranged by size: 

                 184     146     120
 
HindIII A'AGCT_T

Cuts at:      0     336     450 
   Size:        336     114
 
MspA1I CmG'CkG

Cuts at:      0     175     450 
   Size:        175     275
 
PspXI vC'TCGA_Gb

Cuts at:      0      65     450 
   Size:         65     385
 
PstI C_TGCA'G

Cuts at:      0      85     393     450 
   Size:         85     308      57

  Fragments arranged by size: 

                 308      85      57
 
SfcI C'TryA_G

Cuts at:      0      81     389     450 
   Size:         81     308      61

  Fragments arranged by size: 

                 308      81      61
 
SmlI C'TyrA_G

Cuts at:      0      65     330     450 
   Size:         65     265     120

  Fragments arranged by size: 

                 265     120      65
 
TatI w'GTAC_w

Cuts at:      0     350     450 
   Size:        350     100
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     401     433     450 
   Size:        401      32      17

  Fragments arranged by size: 

                 401      32      17
 
Tth111I GACn'n_nGTC

Cuts at:      0     229     450 
   Size:        229     221
 
XbaI T'CTAG_A

Cuts at:      0      72     450 
   Size:         72     378
 
XhoI C'TCGA_G

Cuts at:      0      65     450 
   Size:         65     385
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     AvaI    BamHI     BanI     BbsI    BmgBI
     BpmI    BpuEI    BsaHI    BseRI  BsiHKAI    BsmBI Bsp1286I    BspHI
    BstBI    BstYI   Bsu36I     BtsI    CspCI     EarI  Eco57MI  HindIII
   MspA1I    PspXI     PstI     SfcI     SmlI     TatI     TstI  Tth111I
     XbaI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI
     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BspEI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI
  BstZ17I     BtgI    BtgZI     ClaI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     XcmI     XmaI
     XmnI     ZraI