################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## Acc65I AccIII AclI AflII AhdI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BbeI BbrPI BbvCI BciVI BfrI BglII BlnI BsaAI BsaBI BsaI BseAI BsiWI BsmBI BsmI BspDI BspEI BsrDI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraIII Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRV EcoT22I FspI HindIII HpaI KasI KpnI MamI MfeI MluI MluNI MroI MscI MunI NarI NheI NotI NruI NsiI PacI PaeR7I PflFI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AatII 1640 GACGTC GACGT^C AccI 1953 GTCTAC GT^[AC][GT]AC AflIII 1250 ACATGT A^C[GA][CT]GT AgeI 1995 ACCGGT A^CCGGT Alw44I 2299 GTGCAC G^TGCAC ApaI 256 GGGCCC GGGCC^C ApaLI 2299 GTGCAC G^TGCAC BamHI 1437 GGATCC G^GATCC BanI 118 GGCACC G^G[CT][GA]CC BcgI 1133 GCATGATCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 258 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaWI 1995 ACCGGT [AT]^CCGG[AT] BspHI 572 TCATGA T^CATGA BspLU11I 1250 ACATGT A^CATGT BsrBI 2138 CCGCTC CCGCTC(-3/-3) BstBI 241 TTCGAA TT^CGAA DrdI 2010 GACCAGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 1941 GAATTC G^AATTC FseI 92 GGCCGGCC GGCCGG^CC MaeII 1641 ACGT A^CGT NcoI 520 CCATGG C^CATGG NdeI 863 CATATG CA^TATG NspV 241 TTCGAA TT^CGAA PflMI 1191 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1995 ACCGGT A^CCGGT PpuMI 1712 AGGTCCT [GA]G^G[AT]CC[CT] RcaI 572 TCATGA T^CATGA SapI 2596 GCTCTTC GCTCTTC(1/4) SfuI 241 TTCGAA TT^CGAA SgrAI 1994 CACCGGTG C[GA]^CCGG[CT]G SmlI 1864 CTCAAG C^T[CT][GA]AG SspI 1342 AATATT AAT^ATT Van91I 1191 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 976 CCACCAGATTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AlwNI 1192 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG 2588 CAGCAGCTG Asp700I 725 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1339 GAAAATATTC AvaI 310 CCCGGG C^[CT]CG[GA]G 2339 CCCGGG BclI 569 TGATCA T^GATCA 635 TGATCA BfaI 469 CTAG C^TAG 1771 CTAG BlpI 1128 GCTGAGC GC^T[ACGT]AGC 1461 GCTGAGC Bpu1102I 1128 GCTGAGC GC^T[ACGT]AGC 1461 GCTGAGC BsiEI 95 CGGCCG CG[GA][CT]^CG 101 CGGCCG BsoBI 310 CCCGGG C^[CT]CG[GA]G 2339 CCCGGG Bsp1407I 2000 TGTACA T^GTACA 2121 TGTACA BsrGI 2000 TGTACA T^GTACA 2121 TGTACA BstYI 1437 GGATCC [GA]^GATC[CT] 1821 GGATCT CelII 1128 GCTGAGC GC^T[ACGT]AGC 1461 GCTGAGC DraI 651 TTTAAA TTT^AAA 996 TTTAAA EagI 95 CGGCCG C^GGCCG 101 CGGCCG EclXI 95 CGGCCG C^GGCCG 101 CGGCCG Eco52I 95 CGGCCG C^GGCCG 101 CGGCCG FbaI 569 TGATCA T^GATCA 635 TGATCA HaeII 110 AGCGCC [GA]GCGC^[CT] 2316 GGCGCT KspI 98 CCGCGG CCGC^GG 2102 CCGCGG MaeI 469 CTAG C^TAG 1771 CTAG MflI 1437 GGATCC [GA]^GATC[CT] 1821 GGATCT PshAI 2457 GACGGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2528 GACAGATGTC SacII 98 CCGCGG CCGC^GG 2102 CCGCGG SfaNI 912 GATGC GCATC(5/9) 541 GCATC SmaI 310 CCCGGG CCC^GGG 2339 CCCGGG SspBI 2000 TGTACA T^GTACA 2121 TGTACA XhoII 1437 GGATCC [GA]^GATC[CT] 1821 GGATCT XmaCI 310 CCCGGG C^CCGGG 2339 CCCGGG XmaI 310 CCCGGG C^CCGGG 2339 CCCGGG XmnI 725 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1339 GAAAATATTC ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI ApoI AspHI AvaII BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I