(Linear) (Six-base) MAPSORT of: KIEE5811.seq Check: 937 from: 1 to: 2670 KIEE5811.orf With 182 enzymes: SgfI * December 5, 2014 13:11 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1159 2670 Size: 1159 1511 AatII G_ACGT'C Cuts at: 0 1644 2670 Size: 1644 1026 AccI GT'mk_AC Cuts at: 0 1954 2670 Size: 1954 716 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 755 928 2464 2670 Size: 755 173 1536 206 Fragments arranged by size: 1536 755 206 173 AflIII A'CryG_T Cuts at: 0 1250 2670 Size: 1250 1420 AgeI A'CCGG_T Cuts at: 0 1995 2670 Size: 1995 675 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 931 2670 Size: 931 1739 AlwNI CAG_nnn'CTG Cuts at: 0 1197 2593 2670 Size: 1197 1396 77 Fragments arranged by size: 1396 1197 77 ApaI G_GGCC'C Cuts at: 0 260 2670 Size: 260 2410 ApaLI G'TGCA_C Cuts at: 0 2299 2670 Size: 2299 371 ApoI r'AATT_y Cuts at: 0 322 576 782 890 1791 1941 2664 2670 Size: 322 254 206 108 901 150 723 6 Fragments arranged by size: 901 723 322 254 206 150 108 6 AvaI C'yCGr_G Cuts at: 0 310 2339 2670 Size: 310 2029 331 Fragments arranged by size: 2029 331 310 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2203 2236 2670 Size: 2203 33 434 Fragments arranged by size: 2203 434 33 BamHI G'GATC_C Cuts at: 0 1437 2670 Size: 1437 1233 BanI G'GyrC_C Cuts at: 0 118 2670 Size: 118 2552 BanII G_rGCy'C Cuts at: 0 260 1089 2131 2670 Size: 260 829 1042 539 Fragments arranged by size: 1042 829 539 260 BbsI GAAGACnn'nnnn_ Cuts at: 0 51 251 608 934 2670 Size: 51 200 357 326 1736 Fragments arranged by size: 1736 357 326 200 51 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1122 1156 2670 Size: 1122 34 1514 Fragments arranged by size: 1514 1122 34 BclI T'GATC_A Cuts at: 0 569 635 2670 Size: 569 66 2035 Fragments arranged by size: 2035 569 66 BglI GCCn_nnn'nGGC Cuts at: 0 264 2670 Size: 264 2406 BlpI GC'TnA_GC Cuts at: 0 1129 1462 2670 Size: 1129 333 1208 Fragments arranged by size: 1208 1129 333 Bme1580I G_kGCm'C Cuts at: 0 121 260 759 2303 2670 Size: 121 139 499 1544 367 Fragments arranged by size: 1544 499 367 139 121 BmrI ACTGGGnnnn_n' Cuts at: 0 441 596 685 939 1090 2036 2670 Size: 441 155 89 254 151 946 634 Fragments arranged by size: 946 634 441 254 155 151 89 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1514 1738 2555 2670 Size: 1514 224 817 115 Fragments arranged by size: 1514 817 224 115 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1849 2670 Size: 1849 821 BsaHI Gr'CG_yC Cuts at: 0 73 248 1641 2670 Size: 73 175 1393 1029 Fragments arranged by size: 1393 1029 175 73 BsaWI w'CCGG_w Cuts at: 0 1995 2670 Size: 1995 675 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 148 178 310 340 1974 2004 2020 2050 Size: 148 30 132 30 1634 30 16 30 Cuts at: 2050 2143 2173 2670 Size: 93 30 497 Fragments arranged by size: 1634 497 148 132 93 30 30 30 30 30 16 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 238 1390 1752 2576 2670 Size: 238 1152 362 824 94 Fragments arranged by size: 1152 824 362 238 94 BseYI C'CCAG_C Cuts at: 0 986 2047 2063 2174 2222 2670 Size: 986 1061 16 111 48 448 Fragments arranged by size: 1061 986 448 111 48 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 899 1918 2017 2670 Size: 899 1019 99 653 Fragments arranged by size: 1019 899 653 99 BsiEI CG_ry'CG Cuts at: 0 98 104 2670 Size: 98 6 2566 Fragments arranged by size: 2566 98 6 BsiHKAI G_wGCw'C Cuts at: 0 158 2303 2311 2500 2670 Size: 158 2145 8 189 170 Fragments arranged by size: 2145 189 170 158 8 Bsp1286I G_dGCh'C Cuts at: 0 121 158 260 759 1089 2131 2303 2311 Size: 121 37 102 499 330 1042 172 8 Cuts at: 2311 2500 2670 Size: 189 170 Fragments arranged by size: 1042 499 330 189 172 170 121 102 37 8 BspHI T'CATG_A Cuts at: 0 572 2670 Size: 572 2098 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1159 2039 2199 2670 Size: 1159 880 160 471 Fragments arranged by size: 1159 880 471 160 BsrBI CCG'CTC Cuts at: 0 2140 2670 Size: 2140 530 BsrFI r'CCGG_y Cuts at: 0 93 142 228 1995 2670 Size: 93 49 86 1767 675 Fragments arranged by size: 1767 675 93 86 49 BsrGI T'GTAC_A Cuts at: 0 2000 2121 2670 Size: 2000 121 549 Fragments arranged by size: 2000 549 121 BstBI TT'CG_AA Cuts at: 0 242 2670 Size: 242 2428 BstXI CCAn_nnnn'nTGG Cuts at: 0 693 882 934 986 2670 Size: 693 189 52 52 1684 Fragments arranged by size: 1684 693 189 52 52 BstYI r'GATC_y Cuts at: 0 1437 1821 2670 Size: 1437 384 849 Fragments arranged by size: 1437 849 384 BtgI C'CryG_G Cuts at: 0 98 520 1520 1694 2102 2670 Size: 98 422 1000 174 408 568 Fragments arranged by size: 1000 568 422 408 174 98 BtsI GCAGTG_nn' Cuts at: 0 887 2670 Size: 887 1783 DraI TTT'AAA Cuts at: 0 653 998 2670 Size: 653 345 1672 Fragments arranged by size: 1672 653 345 DrdI GACnn_nn'nnGTC Cuts at: 0 2016 2670 Size: 2016 654 EaeI y'GGCC_r Cuts at: 0 95 101 1170 2135 2670 Size: 95 6 1069 965 535 Fragments arranged by size: 1069 965 535 95 6 EagI C'GGCC_G Cuts at: 0 95 101 2670 Size: 95 6 2569 Fragments arranged by size: 2569 95 6 EarI CTCTTCn'nnn_ Cuts at: 0 30 839 1051 2527 2603 2670 Size: 30 809 212 1476 76 67 Fragments arranged by size: 1476 809 212 76 67 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2349 2670 Size: 2349 321 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 755 928 1514 1738 2464 2555 2670 Size: 755 173 586 224 726 91 115 Fragments arranged by size: 755 726 586 224 173 115 91 EcoO109I rG'GnC_Cy Cuts at: 0 256 257 1713 2670 Size: 256 1 1456 957 Fragments arranged by size: 1456 957 256 1 EcoRI G'AATT_C Cuts at: 0 1941 2670 Size: 1941 729 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2627 2670 Size: 2627 43 FseI GG_CCGG'CC Cuts at: 0 97 2670 Size: 97 2573 HaeII r_GCGC'y Cuts at: 0 114 2320 2670 Size: 114 2206 350 Fragments arranged by size: 2206 350 114 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 664 696 852 884 1487 1519 1974 2006 Size: 664 32 156 32 603 32 455 32 Cuts at: 2006 2670 Size: 664 Fragments arranged by size: 664 664 603 455 156 32 32 32 32 HincII GTy'rAC Cuts at: 0 318 672 1386 2670 Size: 318 354 714 1284 Fragments arranged by size: 1284 714 354 318 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 441 465 1378 2550 2670 Size: 441 24 913 1172 120 Fragments arranged by size: 1172 913 441 120 24 MslI CAynn'nnrTG Cuts at: 0 298 691 907 932 2670 Size: 298 393 216 25 1738 Fragments arranged by size: 1738 393 298 216 25 MspA1I CmG'CkG Cuts at: 0 100 1273 2104 2178 2593 2670 Size: 100 1173 831 74 415 77 Fragments arranged by size: 1173 831 415 100 77 74 NaeI GCC'GGC Cuts at: 0 95 144 230 2670 Size: 95 49 86 2440 Fragments arranged by size: 2440 95 86 49 NcoI C'CATG_G Cuts at: 0 520 2670 Size: 520 2150 NdeI CA'TA_TG Cuts at: 0 864 2670 Size: 864 1806 NgoMIV G'CCGG_C Cuts at: 0 93 142 228 2670 Size: 93 49 86 2442 Fragments arranged by size: 2442 93 86 49 NspI r_CATG'y Cuts at: 0 339 1068 1107 1254 2670 Size: 339 729 39 147 1416 Fragments arranged by size: 1416 729 339 147 39 PciI A'CATG_T Cuts at: 0 1250 2670 Size: 1250 1420 PflMI CCAn_nnn'nTGG Cuts at: 0 1197 2670 Size: 1197 1473 PfoI T'CCnGG_A Cuts at: 0 620 1715 2670 Size: 620 1095 955 Fragments arranged by size: 1095 955 620 PpuMI rG'GwC_Cy Cuts at: 0 1713 2670 Size: 1713 957 PshAI GACnn'nnGTC Cuts at: 0 2461 2532 2670 Size: 2461 71 138 Fragments arranged by size: 2461 138 71 PsiI TTA'TAA Cuts at: 0 451 2670 Size: 451 2219 PspOMI G'GGCC_C Cuts at: 0 256 2670 Size: 256 2414 PstI C_TGCA'G Cuts at: 0 308 390 968 2113 2670 Size: 308 82 578 1145 557 Fragments arranged by size: 1145 578 557 308 82 PvuII CAG'CTG Cuts at: 0 1273 2178 2593 2670 Size: 1273 905 415 77 Fragments arranged by size: 1273 905 415 77 SacII CC_GC'GG Cuts at: 0 101 2105 2670 Size: 101 2004 565 Fragments arranged by size: 2004 565 101 SapI GCTCTTCn'nnn_ Cuts at: 0 2603 2670 Size: 2603 67 SfcI C'TryA_G Cuts at: 0 304 386 964 2109 2670 Size: 304 82 578 1145 561 Fragments arranged by size: 1145 578 561 304 82 SgrAI Cr'CCGG_yG Cuts at: 0 1995 2670 Size: 1995 675 SmaI CCC'GGG Cuts at: 0 312 2341 2670 Size: 312 2029 329 Fragments arranged by size: 2029 329 312 SmlI C'TyrA_G Cuts at: 0 1864 2670 Size: 1864 806 SspI AAT'ATT Cuts at: 0 1344 2670 Size: 1344 1326 StyI C'CwwG_G Cuts at: 0 68 520 1317 2670 Size: 68 452 797 1353 Fragments arranged by size: 1353 797 452 68 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 978 1781 2670 Size: 978 803 889 Fragments arranged by size: 978 889 803 TatI w'GTAC_w Cuts at: 0 2000 2121 2670 Size: 2000 121 549 Fragments arranged by size: 2000 549 121 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 148 180 2143 2175 2670 Size: 148 32 1963 32 495 Fragments arranged by size: 1963 495 148 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 983 2670 Size: 983 1687 XmaI C'CCGG_G Cuts at: 0 310 2339 2670 Size: 310 2029 331 Fragments arranged by size: 2029 331 310 XmnI GAAnn'nnTTC Cuts at: 0 729 1343 2670 Size: 729 614 1327 Fragments arranged by size: 1327 729 614 ZraI GAC'GTC Cuts at: 0 1642 2670 Size: 1642 1028 Enzymes that do cut: AarI AatII AccI AcuI AflIII AgeI AlfI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI BanI BanII BbsI BcgI BclI BglI BlpI Bme1580I BmrI BpmI BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BstBI BstXI BstYI BtgI BtsI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FseI HaeII Hin4I HincII MmeI MslI MspA1I NaeI NcoI NdeI NgoMIV NspI PciI PflMI PfoI PpuMI PshAI PsiI PspOMI PstI PvuII SacII SapI SfcI SgrAI SmaI SmlI SspI StyI TaqII TatI TstI XcmI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AhdI AleI AloI AscI AseI AsiSI AvrII BbeI BbvCI BciVI BfrBI BglII BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsiWI BsmI BsmBI BspEI BsrDI BssHII BssSI BstAPI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII EcoICRI EcoNI EcoRV FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MscI NarI NheI NotI NruI NsiI PacI PasI PmeI PmlI PpiI PspXI PsrI PvuI RsrII SacI SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XbaI XhoI