################################## # NO CUT ENZYMES ( 178 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DpnII DraI DraII DrdI DsaI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI KspI MaeI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MunI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI Sau3AI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 186 CGCG CG^CG AcsI 433 GAATTT [GA]^AATT[CT] AhdI 127 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 433 GAATTT [GA]^AATT[CT] AspEI 127 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 551 GAGCTC G[AT]GC[AT]^C AvaII 299 GGACC G^G[AT]CC BanII 551 GAGCTC G[GA]GC[CT]^C BbsI 90 GTCTTC GAAGAC(2/6) BbvCI 29 CCTCAGC CCTCAGC(-5/-2) BcnI 49 CCCGG CC^[GC]GG BmyI 551 GAGCTC G[AGT]GC[ACT]^C BpuAI 90 GTCTTC GAAGAC(2/6) BsiHKAI 551 GAGCTC G[AT]GC[AT]^C Bsp1286I 551 GAGCTC G[AGT]GC[ACT]^C BsrI 503 ACTGG ACTGG(1/-1) BstF5I 327 CATCC GGATG(2/0) BstNI 330 CCTGG CC^[AT]GG BstUI 186 CGCG CG^CG BstXI 111 CCAAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 185 GCGC GCG^C Cfr13I 299 GGACC G^G[ACGT]CC DraIII 20 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 127 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 153 CTCTTC CTCTTC(1/4) EcoNI 117 CCTTGGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRII 330 CCTGG ^CC[AT]GG EcoT22I 491 ATGCAT ATGCA^T FokI 327 CATCC GGATG(9/13) HaeIII 144 GGCC GG^CC HapII 50 CCGG C^CGG HhaI 185 GCGC GCG^C HinP1I 185 GCGC G^CGC HpaII 50 CCGG C^CGG Ksp632I 153 CTCTTC CTCTTC(1/4) MspI 50 CCGG C^CGG MvaI 330 CCTGG CC^[AT]GG MvnI 186 CGCG CG^CG NciI 49 CCCGG CC^[GC]GG NlaIV 298 GGGACC GG[ACGT]^[ACGT]CC NsiI 491 ATGCAT ATGCA^T NspI 195 GCATGT [GA]CATG^[CT] SacI 551 GAGCTC GAGCT^C Sau96I 299 GGACC G^G[ACGT]CC ScaI 260 AGTACT AGT^ACT SmlI 268 CTCAAG C^T[CT][GA]AG Tsp509I 434 AATT ^AATT ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BciVI 11 GGATAC GTATCC(6/5) 478 GGATAC BpmI 234 CTGGAG CTGGAG(16/14) 331 CTGGAG BsaJI 117 CCTTGG C^C[ACGT][ACGT]GG 556 CCAAGG BseRI 162 CTCCTC GAGGAG(10/8) 171 CTCCTC BsiYI 117 CCTTGGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 556 CCAAGGAGAGG BslI 117 CCTTGGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 556 CCAAGGAGAGG BsmFI 298 GGGAC GGGAC(10/14) 97 GTCCC BspMI 44 ACCTGC ACCTGC(4/8) 106 ACCTGC BssKI 49 CCCGG ^CC[ACGT]GG 330 CCTGG DdeI 30 CTCAG C^T[ACGT]AG 393 CTTAG EcoT14I 117 CCTTGG C^C[AT][AT]GG 556 CCAAGG FauI 187 GCGGG CCCGC(4/6) 296 GCGGG MaeIII 103 GTCAC ^GT[ACGT]AC 135 GTCAC MspA1I 231 CCGCTG C[AC]G^C[GT]G 294 CAGCGG ScrFI 49 CCCGG CC^[ACGT]GG 330 CCTGG SfaNI 326 GCATC GCATC(5/9) 493 GCATC StyI 117 CCTTGG C^C[AT][AT]GG 556 CCAAGG Tsp45I 103 GTCAC ^GT[GC]AC 135 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AciI AfaI AluI BbvI Fnu4HI HphI ItaI MboII MnlI MseI MwoI NlaIII RsaI Tru9I TseI