################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII AccI AccII AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AviII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BclI BfrI BglI BpuAI BsaAI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI Bsp1407I BspEI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I CpoI DraI DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI Ksp632I KspI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### Acc65I 1219 GGTACC G^GTACC Asp700I 1747 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1219 GGTACC G^GTACC AvaI 1506 CCCGGG C^[CT]CG[GA]G BanI 1219 GGTACC G^G[CT][GA]CC BciVI 365 GGATAC GTATCC(6/5) BsaBI 928 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1560 GGTCTC GGTCTC(1/5) BsmI 298 GAATGC GAATGC(1/-1) BsoBI 1506 CCCGGG C^[CT]CG[GA]G BspDI 925 ATCGAT AT^CGAT BspMI 1486 GCAGGT ACCTGC(4/8) Cac8I 1315 GCTTGC GC[ACGT]^[ACGT]GC ClaI 925 ATCGAT AT^CGAT DraIII 159 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 796 CCATGG C^C[GA][CT]GG EcoRI 1003 GAATTC G^AATTC EcoRV 508 GATATC GAT^ATC HindIII 1674 AAGCTT A^AGCTT KpnI 1219 GGTACC GGTAC^C MamI 928 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 796 CCATGG C^CATGG PpuMI 237 AGGACCC [GA]G^G[AT]CC[CT] SmaI 1506 CCCGGG CCC^GGG SspI 822 AATATT AAT^ATT XmaCI 1506 CCCGGG C^CCGGG XmaI 1506 CCCGGG C^CCGGG XmnI 1747 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AflIII 772 ACATGT A^C[GA][CT]GT 1749 ACATGT AlwNI 13 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 1757 CAGGTTCTG AvrII 234 CCTAGG C^CTAGG 1128 CCTAGG BcgI 1452 CGACTTTTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1832 CGACTGCCTTGC BglII 933 AGATCT A^GATCT 1263 AGATCT BlnI 234 CCTAGG C^CTAGG 1128 CCTAGG BlpI 1234 GCTCAGC GC^T[ACGT]AGC 1422 GCTAAGC Bpu1102I 1234 GCTCAGC GC^T[ACGT]AGC 1422 GCTAAGC BspHI 984 TCATGA T^CATGA 1308 TCATGA BspLU11I 772 ACATGT A^CATGT 1749 ACATGT CelII 1234 GCTCAGC GC^T[ACGT]AGC 1422 GCTAAGC DraII 237 AGGACCC [GA]G^G[ACGT]CC[CT] 1814 AGGGCCT EcoNI 1378 CCTACTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1735 CCTGGGACAGG EcoO109I 237 AGGACCC [GA]G^G[ACGT]CC[CT] 1814 AGGGCCT HapII 1507 CCGG C^CGG 1552 CCGG HincII 21 GTTGAC GT[CT]^[GA]AC 1395 GTCAAC HindII 21 GTTGAC GT[CT]^[GA]AC 1395 GTCAAC HpaII 1507 CCGG C^CGG 1552 CCGG MaeII 482 ACGT A^CGT 1155 ACGT MslI 1009 CACAGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1629 CATCCCTGTG MspI 1507 CCGG C^CGG 1552 CCGG RcaI 984 TCATGA T^CATGA 1308 TCATGA StuI 123 AGGCCT AGG^CCT 1732 AGGCCT TaqI 926 TCGA T^CGA 1451 TCGA Tsp45I 1103 GTGAC ^GT[GC]AC 1541 GTGAC TthHB8I 926 TCGA T^CGA 1451 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI AspHI AvaII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TfiI Tru9I TseI Tsp509I TspRI XhoII