(Circular) (Six-base) MAPSORT of: pFN21AE2645.seq Check: 7072 from: 1 to: 6619 pFN21AE2645 6619 bp With 182 enzymes: SgfI * November 16, 2016 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4429 278 Size: 53 83 186 936 2893 2468 Fragments arranged by size: 2893 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3962 1937 Size: 2025 4594 Acc65I G'GTAC_C Cuts at: 3224 3940 3224 Size: 716 5903 AclI AA'CG_TT Cuts at: 1563 4746 5119 1563 Size: 3183 373 3063 Fragments arranged by size: 3183 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3408 4682 6066 6334 1632 Size: 1776 1274 1384 268 1917 Fragments arranged by size: 1917 1776 1384 1274 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6422 AflIII A'CryG_T Cuts at: 1203 2777 3754 5518 6495 1203 Size: 1574 977 1764 977 1327 Fragments arranged by size: 1764 1574 1327 977 977 AgeI A'CCGG_T Cuts at: 5450 5450 Size: 6619 AhdI GACnn_n'nnGTC Cuts at: 5348 5348 Size: 6619 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6619 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6619 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3741 3773 3741 Size: 32 6587 AlwNI CAG_nnn'CTG Cuts at: 2023 3767 4362 5934 2023 Size: 1744 595 1572 2708 Fragments arranged by size: 2708 1744 1572 595 ApaLI G'TGCA_C Cuts at: 4676 5832 4676 Size: 1156 5463 ApoI r'AATT_y Cuts at: 1493 2140 2159 3008 3035 3928 4154 4306 6415 Size: 647 19 849 27 893 226 152 2109 Cuts at: 6415 1493 Size: 1697 Fragments arranged by size: 2109 1697 893 849 647 226 152 27 19 AseI AT'TA_AT Cuts at: 160 1032 5173 160 Size: 872 4141 1606 Fragments arranged by size: 4141 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6619 AvaI C'yCGr_G Cuts at: 1870 1943 3511 3944 1870 Size: 73 1568 433 4545 Fragments arranged by size: 4545 1568 433 73 AvrII C'CTAG_G Cuts at: 2239 3133 2239 Size: 894 5725 BamHI G'GATC_C Cuts at: 3949 4316 3949 Size: 367 6252 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3224 3940 5395 618 Size: 325 217 627 1437 716 1455 1842 Fragments arranged by size: 1842 1455 1437 716 627 325 217 BanII G_rGCy'C Cuts at: 1391 3938 1391 Size: 2547 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5648 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3446 3480 3826 3860 4810 4844 1903 Size: 34 1509 34 346 34 950 34 3678 Fragments arranged by size: 3678 1509 950 346 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2364 4511 5721 2364 Size: 2147 1210 3262 Fragments arranged by size: 3262 2147 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6619 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5230 136 Size: 107 122 71 631 605 3558 1525 Fragments arranged by size: 3558 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2938 3268 6614 2938 Size: 330 3346 2943 Fragments arranged by size: 3346 2943 330 BlpI GC'TnA_GC Cuts at: 3240 3428 4036 3240 Size: 188 608 5823 Fragments arranged by size: 5823 608 188 Bme1580I G_kGCm'C Cuts at: 1790 4680 5836 1790 Size: 2890 1156 2573 Fragments arranged by size: 2890 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6619 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2508 2897 3336 Size: 416 53 83 399 24 1091 389 439 Cuts at: 3336 4350 5308 442 Size: 1014 958 1753 Fragments arranged by size: 1753 1091 1014 958 439 416 399 389 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6619 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3244 3886 3244 Size: 642 5977 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2125 2594 3209 5279 1239 Size: 886 469 615 2070 2579 Fragments arranged by size: 2579 2070 886 615 469 Bpu10I CC'TnA_GC Cuts at: 2090 2181 2090 Size: 91 6528 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2262 2274 2930 4744 5609 5907 2262 Size: 12 656 1814 865 298 2974 Fragments arranged by size: 2974 1814 865 656 298 12 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3571 5282 882 Size: 617 2072 1711 2219 Fragments arranged by size: 2219 2072 1711 617 BsaAI yAC'GTr Cuts at: 493 1204 4410 493 Size: 711 3206 2702 Fragments arranged by size: 3206 2702 711 BsaBI GATnn'nnATC Cuts at: 2937 6613 2937 Size: 3676 2943 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4426 4808 5443 275 Size: 53 83 186 936 2893 382 635 1451 Fragments arranged by size: 2893 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5052 5450 5724 5871 6282 5052 Size: 398 274 147 411 5389 Fragments arranged by size: 5389 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3741 3771 3776 3806 1178 Size: 30 165 30 2338 30 5 30 3991 Fragments arranged by size: 3991 2338 165 30 30 30 30 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2933 2978 3636 1186 Size: 1747 45 658 4169 Fragments arranged by size: 4169 1747 658 45 BseYI C'CCAG_C Cuts at: 2324 3731 5822 6318 2324 Size: 1407 2091 496 2625 Fragments arranged by size: 2625 2091 1407 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6046 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4069 4830 5858 1540 Size: 275 186 2068 761 1028 2301 Fragments arranged by size: 2301 2068 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2101 2745 3893 4680 4765 5836 2101 Size: 644 1148 787 85 1071 2884 Fragments arranged by size: 2884 1148 1071 787 644 85 BsmI GAATG_Cn' Cuts at: 2309 4130 4223 2309 Size: 1821 93 4705 Fragments arranged by size: 4705 1821 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2101 2745 3893 3938 4680 4765 5836 Size: 399 311 644 1148 45 742 85 1071 Cuts at: 5836 1391 Size: 2174 Fragments arranged by size: 2174 1148 1071 742 644 399 311 85 45 BspEI T'CCGG_A Cuts at: 6282 6282 Size: 6619 BspHI T'CATG_A Cuts at: 2989 3313 4508 6238 2989 Size: 324 1195 1730 3370 Fragments arranged by size: 3370 1730 1195 324 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3482 3974 844 Size: 2638 492 3489 Fragments arranged by size: 3489 2638 492 BsrBI CCG'CTC Cuts at: 1394 1823 4506 1394 Size: 429 2683 3507 Fragments arranged by size: 3507 2683 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2404 2460 2548 5114 5288 65 Size: 1183 1156 56 88 2566 174 1396 Fragments arranged by size: 2566 1396 1183 1156 174 88 56 BsrFI r'CCGG_y Cuts at: 1700 1810 5263 5450 1700 Size: 110 3453 187 2869 Fragments arranged by size: 3453 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6619 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6619 BssSI C'ACGA_G Cuts at: 4679 5691 4679 Size: 1012 5607 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6619 BstBI TT'CG_AA Cuts at: 5423 5423 Size: 6619 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6619 BstYI r'GATC_y Cuts at: 1972 2008 2452 2696 2938 3268 3949 4316 4701 Size: 36 444 244 242 330 681 367 385 Cuts at: 4701 4718 6170 6256 6268 6614 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 681 444 385 367 346 330 244 242 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2801 513 Size: 552 939 797 4331 Fragments arranged by size: 4331 939 797 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5880 Fragments arranged by size: 5880 683 56 BtsI GCAGTG_nn' Cuts at: 3112 3723 4135 4929 4949 3112 Size: 611 412 794 20 4782 Fragments arranged by size: 4782 794 611 412 20 ClaI AT'CG_AT Cuts at: 1554 2931 1554 Size: 1377 5242 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6584 DraI TTT'AAA Cuts at: 3923 3923 Size: 6619 DraIII CAC_nnn'GTG Cuts at: 2169 2169 Size: 6619 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5626 809 Size: 507 4310 1802 Fragments arranged by size: 4310 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4066 4955 6584 Size: 54 1425 50 275 18 2236 889 1629 Cuts at: 6584 8 Size: 43 Fragments arranged by size: 2236 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4066 1537 Size: 275 2254 4090 Fragments arranged by size: 4090 2254 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4549 1353 Size: 3196 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5206 5580 5726 387 Size: 1064 138 3617 374 146 1280 Fragments arranged by size: 3617 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2125 2594 3209 3408 4682 5279 6066 Size: 393 493 469 615 199 1274 597 787 Cuts at: 6066 6334 1239 Size: 268 1524 Fragments arranged by size: 1524 1274 787 615 597 493 469 393 268 199 EcoNI CCTnn'n_nnAGG Cuts at: 3387 3744 3387 Size: 357 6262 EcoO109I rG'GnC_Cy Cuts at: 2243 3820 2243 Size: 1577 5042 EcoRI G'AATT_C Cuts at: 3008 3928 4306 3008 Size: 920 378 5321 Fragments arranged by size: 5321 920 378 EcoRV GAT'ATC Cuts at: 2515 2515 Size: 6619 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2073 2073 Size: 6619 FspI TGC'GCA Cuts at: 5125 5125 Size: 6619 HaeII r_GCGC'y Cuts at: 1678 5766 1678 Size: 4088 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3137 3169 3776 3808 5260 Size: 32 135 32 1565 32 607 32 1452 Cuts at: 5260 5292 5334 5366 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1565 1452 607 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2028 3402 3963 4209 669 Size: 988 281 90 1374 561 246 3079 Fragments arranged by size: 3079 1374 988 561 281 246 90 HindIII A'AGCT_T Cuts at: 748 3679 748 Size: 2931 3688 HpaI GTT'AAC Cuts at: 4209 4209 Size: 6619 KpnI G_GTAC'C Cuts at: 3228 3944 3228 Size: 716 5903 MfeI C'AATT_G Cuts at: 4218 4218 Size: 6619 MluI A'CGCG_T Cuts at: 6495 6495 Size: 6619 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5733 5917 1305 Size: 4428 184 2007 Fragments arranged by size: 4428 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4797 Fragments arranged by size: 4797 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3018 3638 4577 4936 5095 518 Size: 648 1852 620 939 359 159 2042 Fragments arranged by size: 2042 1852 939 648 620 359 159 MspA1I CmG'CkG Cuts at: 1606 2020 2254 2337 3168 4035 4712 5860 6105 Size: 414 234 83 831 867 677 1148 245 Cuts at: 6105 1606 Size: 2120 Fragments arranged by size: 2120 1148 867 831 677 414 245 234 83 NcoI C'CATG_G Cuts at: 513 1065 2004 2801 513 Size: 552 939 797 4331 Fragments arranged by size: 4331 939 797 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6619 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6619 NotI GC'GGCC_GC Cuts at: 4066 4066 Size: 6619 NspI r_CATG'y Cuts at: 1132 1229 2781 3553 3758 3977 5522 1132 Size: 97 1552 772 205 219 1545 2229 Fragments arranged by size: 2229 1552 1545 772 219 205 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6619 PciI A'CATG_T Cuts at: 2777 3754 5518 2777 Size: 977 1764 3878 Fragments arranged by size: 3878 1764 977 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6619 PmeI GTTT'AAAC Cuts at: 3923 3923 Size: 6619 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3741 3773 4688 4720 6201 6233 3741 Size: 32 915 32 1481 32 4127 Fragments arranged by size: 4127 1481 915 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2243 2243 Size: 6619 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6619 PsiI TTA'TAA Cuts at: 4189 4189 Size: 6619 PstI C_TGCA'G Cuts at: 830 3971 830 Size: 3141 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6619 PvuII CAG'CTG Cuts at: 2020 2254 2337 3168 2020 Size: 234 83 831 5471 Fragments arranged by size: 5471 831 234 83 SalI G'TCGA_C Cuts at: 1936 3961 1936 Size: 2025 4594 SbfI CC_TGCA'GG Cuts at: 3971 3971 Size: 6619 ScaI AGT'ACT Cuts at: 4867 6579 4867 Size: 1712 4907 SfcI C'TryA_G Cuts at: 826 1045 3967 5102 5783 5974 826 Size: 219 2922 1135 681 191 1471 Fragments arranged by size: 2922 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6619 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6619 SmaI CCC'GGG Cuts at: 1872 3513 3946 1872 Size: 1641 433 4545 Fragments arranged by size: 4545 1641 433 SmlI C'TyrA_G Cuts at: 820 1017 1943 2277 2289 2945 4723 5624 5886 Size: 197 926 334 12 656 1778 901 262 Cuts at: 5886 820 Size: 1553 Fragments arranged by size: 1778 1553 926 901 656 334 262 197 12 SnaBI TAC'GTA Cuts at: 493 4410 493 Size: 3917 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6619 SphI G_CATG'C Cuts at: 1132 3977 1132 Size: 2845 3774 SspI AAT'ATT Cuts at: 5 52 2829 4543 5 Size: 47 2777 1714 2081 Fragments arranged by size: 2777 2081 1714 47 StuI AGG'CCT Cuts at: 1517 2130 3739 1517 Size: 613 1609 4397 Fragments arranged by size: 4397 1609 613 StyI C'CwwG_G Cuts at: 513 1065 2004 2031 2239 2801 3133 4058 513 Size: 552 939 27 208 562 332 925 3074 Fragments arranged by size: 3074 939 925 562 552 332 208 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2473 4646 4663 4816 5001 5446 325 Size: 1255 893 2173 17 153 185 445 1498 Fragments arranged by size: 2173 1498 1255 893 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2655 3805 4865 Size: 274 80 33 51 1443 678 1150 1060 Cuts at: 4865 6577 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1150 1060 678 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2405 2437 6579 6611 2405 Size: 32 4142 32 2413 Fragments arranged by size: 4142 2413 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6619 XbaI T'CTAG_A Cuts at: 3955 3955 Size: 6619 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6619 XmaI C'CCGG_G Cuts at: 1870 3511 3944 1870 Size: 1641 433 4545 Fragments arranged by size: 4545 1641 433 XmnI GAAnn'nnTTC Cuts at: 3756 4748 3756 Size: 992 5627 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4427 276 Size: 53 83 186 936 2893 2468 Fragments arranged by size: 2893 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI