Restriction map for
pFN21AE2645
(Linear) (Six-base) MAPSORT of: KIEE2645.seq Check: 317 from: 1 to: 1914
KIEE2645.orf
With 182 enzymes: SgfI *
November 16, 2016 14:56 ..
Acc65I G'GTAC_C
Cuts at: 0 1219 1914
Size: 1219 695
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1403 1914
Size: 1403 511
AflIII A'CryG_T
Cuts at: 0 772 1749 1914
Size: 772 977 165
Fragments arranged by size:
977 772 165
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1736 1768 1914
Size: 1736 32 146
Fragments arranged by size:
1736 146 32
AlwNI CAG_nnn'CTG
Cuts at: 0 18 1762 1914
Size: 18 1744 152
Fragments arranged by size:
1744 152 18
ApoI r'AATT_y
Cuts at: 0 135 154 1003 1030 1914
Size: 135 19 849 27 884
Fragments arranged by size:
884 849 135 27 19
AvaI C'yCGr_G
Cuts at: 0 1506 1914
Size: 1506 408
AvrII C'CTAG_G
Cuts at: 0 234 1128 1914
Size: 234 894 786
Fragments arranged by size:
894 786 234
BanI G'GyrC_C
Cuts at: 0 1219 1914
Size: 1219 695
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1441 1475 1821 1855 1914
Size: 1441 34 346 34 59
Fragments arranged by size:
1441 346 59 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 359 1914
Size: 359 1555
BglII A'GATC_T
Cuts at: 0 933 1263 1914
Size: 933 330 651
Fragments arranged by size:
933 651 330
BlpI GC'TnA_GC
Cuts at: 0 1235 1423 1914
Size: 1235 188 491
Fragments arranged by size:
1235 491 188
BmrI ACTGGGnnnn_n'
Cuts at: 0 503 892 1331 1914
Size: 503 389 439 583
Fragments arranged by size:
583 503 439 389
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1239 1881 1914
Size: 1239 642 33
Fragments arranged by size:
1239 642 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 589 1204 1914
Size: 120 469 615 710
Fragments arranged by size:
710 615 469 120
Bpu10I CC'TnA_GC
Cuts at: 0 85 176 1914
Size: 85 91 1738
Fragments arranged by size:
1738 91 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 269 925 1914
Size: 257 12 656 989
Fragments arranged by size:
989 656 257 12
BsaI GGTCTCn'nnnn_
Cuts at: 0 1566 1914
Size: 1566 348
BsaBI GATnn'nnATC
Cuts at: 0 932 1914
Size: 932 982
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1736 1766 1771 1801 1914
Size: 1736 30 5 30 113
Fragments arranged by size:
1736 113 30 30 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 928 973 1631 1914
Size: 928 45 658 283
Fragments arranged by size:
928 658 283 45
BseYI C'CCAG_C
Cuts at: 0 319 1726 1914
Size: 319 1407 188
Fragments arranged by size:
1407 319 188
BsiHKAI G_wGCw'C
Cuts at: 0 96 740 1888 1914
Size: 96 644 1148 26
Fragments arranged by size:
1148 644 96 26
BsmI GAATG_Cn'
Cuts at: 0 304 1914
Size: 304 1610
Bsp1286I G_dGCh'C
Cuts at: 0 96 740 1888 1914
Size: 96 644 1148 26
Fragments arranged by size:
1148 644 96 26
BspHI T'CATG_A
Cuts at: 0 984 1308 1914
Size: 984 324 606
Fragments arranged by size:
984 606 324
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1477 1914
Size: 1477 437
BsrDI GCAATG_nn'
Cuts at: 0 399 455 543 1914
Size: 399 56 88 1371
Fragments arranged by size:
1371 399 88 56
BstYI r'GATC_y
Cuts at: 0 3 447 691 933 1263 1914
Size: 3 444 244 242 330 651
Fragments arranged by size:
651 444 330 244 242 3
BtgI C'CryG_G
Cuts at: 0 796 1914
Size: 796 1118
BtsI GCAGTG_nn'
Cuts at: 0 1107 1718 1914
Size: 1107 611 196
Fragments arranged by size:
1107 611 196
ClaI AT'CG_AT
Cuts at: 0 926 1914
Size: 926 988
DraIII CAC_nnn'GTG
Cuts at: 0 164 1914
Size: 164 1750
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 589 1204 1403 1914
Size: 120 469 615 199 511
Fragments arranged by size:
615 511 469 199 120
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1382 1739 1914
Size: 1382 357 175
Fragments arranged by size:
1382 357 175
EcoO109I rG'GnC_Cy
Cuts at: 0 238 1815 1914
Size: 238 1577 99
Fragments arranged by size:
1577 238 99
EcoRI G'AATT_C
Cuts at: 0 1003 1914
Size: 1003 911
EcoRV GAT'ATC
Cuts at: 0 510 1914
Size: 510 1404
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 68 1914
Size: 68 1846
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1132 1164 1771 1803 1914
Size: 1132 32 607 32 111
Fragments arranged by size:
1132 607 111 32 32
HincII GTy'rAC
Cuts at: 0 23 1397 1914
Size: 23 1374 517
Fragments arranged by size:
1374 517 23
HindIII A'AGCT_T
Cuts at: 0 1674 1914
Size: 1674 240
KpnI G_GTAC'C
Cuts at: 0 1223 1914
Size: 1223 691
MslI CAynn'nnrTG
Cuts at: 0 1013 1633 1914
Size: 1013 620 281
Fragments arranged by size:
1013 620 281
MspA1I CmG'CkG
Cuts at: 0 15 249 332 1163 1914
Size: 15 234 83 831 751
Fragments arranged by size:
831 751 234 83 15
NcoI C'CATG_G
Cuts at: 0 796 1914
Size: 796 1118
NspI r_CATG'y
Cuts at: 0 776 1548 1753 1914
Size: 776 772 205 161
Fragments arranged by size:
776 772 205 161
PciI A'CATG_T
Cuts at: 0 772 1749 1914
Size: 772 977 165
Fragments arranged by size:
977 772 165
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1736 1768 1914
Size: 1736 32 146
Fragments arranged by size:
1736 146 32
PpuMI rG'GwC_Cy
Cuts at: 0 238 1914
Size: 238 1676
PvuII CAG'CTG
Cuts at: 0 15 249 332 1163 1914
Size: 15 234 83 831 751
Fragments arranged by size:
831 751 234 83 15
SmaI CCC'GGG
Cuts at: 0 1508 1914
Size: 1508 406
SmlI C'TyrA_G
Cuts at: 0 272 284 940 1914
Size: 272 12 656 974
Fragments arranged by size:
974 656 272 12
SspI AAT'ATT
Cuts at: 0 824 1914
Size: 824 1090
StuI AGG'CCT
Cuts at: 0 125 1734 1914
Size: 125 1609 180
Fragments arranged by size:
1609 180 125
StyI C'CwwG_G
Cuts at: 0 26 234 796 1128 1914
Size: 26 208 562 332 786
Fragments arranged by size:
786 562 332 208 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 468 1914
Size: 468 1446
TatI w'GTAC_w
Cuts at: 0 650 1800 1914
Size: 650 1150 114
Fragments arranged by size:
1150 650 114
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 400 432 1914
Size: 400 32 1482
Fragments arranged by size:
1482 400 32
XmaI C'CCGG_G
Cuts at: 0 1506 1914
Size: 1506 408
XmnI GAAnn'nnTTC
Cuts at: 0 1751 1914
Size: 1751 163
Enzymes that do cut:
Acc65I AcuI AflIII AloI AlwNI ApoI AvaI AvrII
BanI BcgI BciVI BglII BlpI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaXI BseRI BseYI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrDI BstYI BtgI BtsI
ClaI DraIII Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
Hin4I HincII HindIII KpnI MslI MspA1I NcoI NspI
PciI PpiI PpuMI PvuII SmaI SmlI SspI StuI
StyI TaqII TatI TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AseI AsiSI BaeI
BamHI BanII BbeI BbsI BbvCI BclI BfrBI BglI
Bme1580I BmgBI BmtI BsaAI BsaHI BsaWI BsgI BsiEI
BsiWI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI FseI
FspI FspAI HaeII HpaI KasI MfeI MluI MmeI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SwaI Tth111I XbaI XcmI XhoI ZraI