(Circular) (Six-base) MAPSORT of: pFN21AE9567.seq Check: 4422 from: 1 to: 6169 pFN21AE9567 6169 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3979 278 Size: 53 83 186 936 2443 2468 Fragments arranged by size: 2468 2443 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3512 1937 Size: 1575 4594 Acc65I G'GTAC_C Cuts at: 3490 3490 Size: 6169 AclI AA'CG_TT Cuts at: 1563 4296 4669 1563 Size: 2733 373 3063 Fragments arranged by size: 3063 2733 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2293 2560 3179 4232 5616 5884 1632 Size: 661 267 619 1053 1384 268 1917 Fragments arranged by size: 1917 1384 1053 661 619 268 267 AfeI AGC'GCT Cuts at: 2354 3077 2354 Size: 723 5446 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5972 AflIII A'CryG_T Cuts at: 1203 2562 5068 6045 1203 Size: 1359 2506 977 1327 Fragments arranged by size: 2506 1359 1327 977 AgeI A'CCGG_T Cuts at: 5000 5000 Size: 6169 AhdI GACnn_n'nnGTC Cuts at: 4898 4898 Size: 6169 AleI CACnn'nnGTG Cuts at: 1166 2534 1166 Size: 1368 4801 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3260 1184 Size: 2076 4093 AlwNI CAG_nnn'CTG Cuts at: 2275 3912 5484 2275 Size: 1637 1572 2960 Fragments arranged by size: 2960 1637 1572 ApaLI G'TGCA_C Cuts at: 4226 5382 4226 Size: 1156 5013 ApoI r'AATT_y Cuts at: 1493 3241 3478 3704 3856 5965 1493 Size: 1748 237 226 152 2109 1697 Fragments arranged by size: 2109 1748 1697 237 226 152 AseI AT'TA_AT Cuts at: 160 1032 4723 160 Size: 872 3691 1606 Fragments arranged by size: 3691 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6169 AvaI C'yCGr_G Cuts at: 1870 1943 2015 2261 2718 3150 3494 1870 Size: 73 72 246 457 432 344 4545 Fragments arranged by size: 4545 457 432 344 246 73 72 BamHI G'GATC_C Cuts at: 3499 3866 3499 Size: 367 5802 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2675 2769 2798 3490 4945 Size: 325 217 627 888 94 29 692 1455 Cuts at: 4945 618 Size: 1842 Fragments arranged by size: 1842 1455 888 692 627 325 217 94 29 BanII G_rGCy'C Cuts at: 1391 2045 2225 3416 3488 1391 Size: 654 180 1191 72 4072 Fragments arranged by size: 4072 1191 654 180 72 BbeI G_GCGC'C Cuts at: 2773 2802 2773 Size: 29 6140 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3288 928 Size: 971 1389 3809 Fragments arranged by size: 3809 1389 971 BbvCI CC'TCA_GC Cuts at: 2324 2756 2324 Size: 432 5737 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4360 4394 1903 Size: 34 2423 34 3678 Fragments arranged by size: 3678 2423 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4061 5271 4061 Size: 1210 4959 BclI T'GATC_A Cuts at: 1548 1548 Size: 6169 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2319 3057 3234 Size: 107 122 71 631 605 647 738 177 Cuts at: 3234 4780 136 Size: 1546 1525 Fragments arranged by size: 1546 1525 738 647 631 605 177 122 107 71 BglII A'GATC_T Cuts at: 6164 6164 Size: 6169 BlpI GC'TnA_GC Cuts at: 3586 3586 Size: 6169 Bme1580I G_kGCm'C Cuts at: 1790 2210 2678 4230 5386 1790 Size: 420 468 1552 1156 2573 Fragments arranged by size: 2573 1552 1156 468 420 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6169 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2057 3429 3900 Size: 416 53 83 399 24 640 1372 471 Cuts at: 3900 4858 442 Size: 958 1753 Fragments arranged by size: 1753 1372 958 640 471 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6169 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4829 1239 Size: 3590 2579 Bpu10I CC'TnA_GC Cuts at: 2324 2756 2324 Size: 432 5737 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2059 4294 5159 5457 2059 Size: 2235 865 298 2771 Fragments arranged by size: 2771 2235 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2480 3311 4832 882 Size: 617 981 831 1521 2219 Fragments arranged by size: 2219 1521 981 831 617 BsaAI yAC'GTr Cuts at: 493 1204 2241 2395 3960 493 Size: 711 1037 154 1565 2702 Fragments arranged by size: 2702 1565 1037 711 154 BsaBI GATnn'nnATC Cuts at: 2087 2816 2990 6163 2087 Size: 729 174 3173 2093 Fragments arranged by size: 3173 2093 729 174 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2770 2799 3976 4358 Size: 53 83 186 936 1237 29 1177 382 Cuts at: 4358 4993 275 Size: 635 1451 Fragments arranged by size: 1451 1237 1177 936 635 382 186 83 53 29 BsaWI w'CCGG_w Cuts at: 3080 4602 5000 5274 5421 5832 3080 Size: 1522 398 274 147 411 3417 Fragments arranged by size: 3417 1522 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3122 3152 1178 Size: 30 165 30 1719 30 4195 Fragments arranged by size: 4195 1719 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6169 BseYI C'CCAG_C Cuts at: 2671 2740 3392 5372 5868 2671 Size: 69 652 1980 496 2972 Fragments arranged by size: 2972 1980 652 496 69 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5596 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2050 2697 2703 3619 4380 5408 Size: 275 186 49 647 6 916 761 1028 Cuts at: 5408 1540 Size: 2301 Fragments arranged by size: 2301 1028 916 761 647 275 186 49 6 BsiHKAI G_wGCw'C Cuts at: 2451 2850 3015 3416 4230 4315 5386 2451 Size: 399 165 401 814 85 1071 3234 Fragments arranged by size: 3234 1071 814 401 399 165 85 BsmI GAATG_Cn' Cuts at: 3680 3773 3680 Size: 93 6076 BsmBI CGTCTCn'nnnn_ Cuts at: 2150 2150 Size: 6169 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2210 2225 2451 2678 2850 3015 Size: 399 255 165 15 226 227 172 165 Cuts at: 3015 3416 3488 4230 4315 5386 1391 Size: 401 72 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 401 399 255 227 226 172 165 165 85 72 15 BspEI T'CCGG_A Cuts at: 3080 5832 3080 Size: 2752 3417 BspHI T'CATG_A Cuts at: 4058 5788 4058 Size: 1730 4439 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3524 844 Size: 2680 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2866 3384 4056 1394 Size: 429 1043 518 672 3507 Fragments arranged by size: 3507 1043 672 518 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2826 4664 4838 65 Size: 1183 1578 1838 174 1396 Fragments arranged by size: 1838 1578 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2049 4813 5000 1700 Size: 110 239 2764 187 2869 Fragments arranged by size: 2869 2764 239 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6169 BssHII G'CGCG_C Cuts at: 1925 2457 2628 1925 Size: 532 171 5466 Fragments arranged by size: 5466 532 171 BssSI C'ACGA_G Cuts at: 2867 4229 5241 2867 Size: 1362 1012 3795 Fragments arranged by size: 3795 1362 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2334 2435 794 Size: 1540 101 4528 Fragments arranged by size: 4528 1540 101 BstBI TT'CG_AA Cuts at: 4973 4973 Size: 6169 BstEII G'GTnAC_C Cuts at: 1184 2227 1184 Size: 1043 5126 BstXI CCAn_nnnn'nTGG Cuts at: 2508 2839 2508 Size: 331 5838 BstYI r'GATC_y Cuts at: 1972 2805 3499 3866 4251 4268 5720 5806 5818 Size: 833 694 367 385 17 1452 86 12 Cuts at: 5818 6164 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 833 694 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3405 3405 Size: 6169 BtgI C'CryG_G Cuts at: 513 1065 2697 513 Size: 552 1632 3985 Fragments arranged by size: 3985 1632 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2218 2776 487 Size: 683 56 992 558 3880 Fragments arranged by size: 3880 992 683 558 56 BtsI GCAGTG_nn' Cuts at: 3088 3203 3685 4479 4499 3088 Size: 115 482 794 20 4758 Fragments arranged by size: 4758 794 482 115 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6169 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6134 DraI TTT'AAA Cuts at: 3473 3473 Size: 6169 DraIII CAC_nnn'GTG Cuts at: 2395 2511 2395 Size: 116 6053 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5176 809 Size: 507 3860 1802 Fragments arranged by size: 3860 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2115 2361 2694 Size: 54 1425 50 275 18 285 246 333 Cuts at: 2694 2700 3616 4505 6134 8 Size: 6 916 889 1629 43 Fragments arranged by size: 1629 1425 916 889 333 285 275 246 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2694 2700 3616 1537 Size: 275 882 6 916 4090 Fragments arranged by size: 4090 916 882 275 6 EarI CTCTTCn'nnn_ Cuts at: 1353 2309 3050 3334 4099 1353 Size: 956 741 284 765 3423 Fragments arranged by size: 3423 956 765 741 284 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2020 2774 4756 5130 5276 387 Size: 1064 138 431 754 1982 374 146 1280 Fragments arranged by size: 1982 1280 1064 754 431 374 146 138 EcoICRI GAG'CTC Cuts at: 3414 3414 Size: 6169 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2293 2560 3179 4232 4829 5616 5884 Size: 393 661 267 619 1053 597 787 268 Cuts at: 5884 1239 Size: 1524 Fragments arranged by size: 1524 1053 787 661 619 597 393 268 267 EcoNI CCTnn'n_nnAGG Cuts at: 3275 3295 3275 Size: 20 6149 EcoO109I rG'GnC_Cy Cuts at: 3063 3063 Size: 6169 EcoRI G'AATT_C Cuts at: 3478 3856 3478 Size: 378 5791 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3129 3129 Size: 6169 FspI TGC'GCA Cuts at: 4675 4675 Size: 6169 HaeII r_GCGC'y Cuts at: 1678 2356 2773 2802 3079 5316 1678 Size: 678 417 29 277 2237 2531 Fragments arranged by size: 2531 2237 678 417 277 29 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2868 2900 3275 3307 4810 Size: 32 135 32 1296 32 375 32 1503 Cuts at: 4810 4842 4884 4916 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1503 1296 375 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2887 3513 3759 669 Size: 988 281 949 626 246 3079 Fragments arranged by size: 3079 988 949 626 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6169 HpaI GTT'AAC Cuts at: 3759 3759 Size: 6169 KasI G'GCGC_C Cuts at: 2769 2798 2769 Size: 29 6140 KpnI G_GTAC'C Cuts at: 3494 3494 Size: 6169 MfeI C'AATT_G Cuts at: 3768 3768 Size: 6169 MluI A'CGCG_T Cuts at: 6045 6045 Size: 6169 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2466 5283 5467 1305 Size: 1161 2817 184 2007 Fragments arranged by size: 2817 2007 1161 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2117 2363 10 Size: 54 1425 343 285 246 3816 Fragments arranged by size: 3816 1425 343 285 246 54 MslI CAynn'nnrTG Cuts at: 518 1166 2123 2243 2534 4127 4486 4645 518 Size: 648 957 120 291 1593 359 159 2042 Fragments arranged by size: 2042 1593 957 648 359 291 159 120 MspA1I CmG'CkG Cuts at: 1606 2275 2699 2732 3585 4262 5410 5655 1606 Size: 669 424 33 853 677 1148 245 2120 Fragments arranged by size: 2120 1148 853 677 669 424 245 33 NarI GG'CG_CC Cuts at: 2770 2799 2770 Size: 29 6140 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5617 NdeI CA'TA_TG Cuts at: 387 387 Size: 6169 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6169 NotI GC'GGCC_GC Cuts at: 2694 2700 3616 2694 Size: 6 916 5247 Fragments arranged by size: 5247 916 6 NspI r_CATG'y Cuts at: 1132 1229 2566 2644 3527 5072 1132 Size: 97 1337 78 883 1545 2229 Fragments arranged by size: 2229 1545 1337 883 97 78 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6169 PciI A'CATG_T Cuts at: 2562 5068 2562 Size: 2506 3663 PflMI CCAn_nnn'nTGG Cuts at: 1108 2395 1108 Size: 1287 4882 PmeI GTTT'AAAC Cuts at: 3473 3473 Size: 6169 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4238 4270 5751 5783 4238 Size: 32 1481 32 4624 Fragments arranged by size: 4624 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3063 3063 Size: 6169 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6169 PsiI TTA'TAA Cuts at: 3739 3739 Size: 6169 PstI C_TGCA'G Cuts at: 830 2496 3521 830 Size: 1666 1025 3478 Fragments arranged by size: 3478 1666 1025 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6169 SacI G_AGCT'C Cuts at: 3416 3416 Size: 6169 SacII CC_GC'GG Cuts at: 2700 2700 Size: 6169 SalI G'TCGA_C Cuts at: 1936 3511 1936 Size: 1575 4594 SanDI GG'GwC_CC Cuts at: 3063 3063 Size: 6169 SapI GCTCTTCn'nnn_ Cuts at: 2309 2309 Size: 6169 SbfI CC_TGCA'GG Cuts at: 3521 3521 Size: 6169 ScaI AGT'ACT Cuts at: 4417 6129 4417 Size: 1712 4457 SexAI A'CCwGG_T Cuts at: 2109 2507 2109 Size: 398 5771 SfcI C'TryA_G Cuts at: 826 1045 2383 2492 3517 4652 5333 5524 826 Size: 219 1338 109 1025 1135 681 191 1471 Fragments arranged by size: 1471 1338 1135 1025 681 219 191 109 SfoI GGC'GCC Cuts at: 2771 2800 2771 Size: 29 6140 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6169 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6169 SmaI CCC'GGG Cuts at: 1872 2017 2263 2720 3152 3496 1872 Size: 145 246 457 432 344 4545 Fragments arranged by size: 4545 457 432 344 246 145 SmlI C'TyrA_G Cuts at: 820 1017 1943 2038 4273 5174 5436 820 Size: 197 926 95 2235 901 262 1553 Fragments arranged by size: 2235 1553 926 901 262 197 95 SnaBI TAC'GTA Cuts at: 493 3960 493 Size: 3467 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6169 SphI G_CATG'C Cuts at: 1132 2644 3527 1132 Size: 1512 883 3774 Fragments arranged by size: 3774 1512 883 SspI AAT'ATT Cuts at: 5 52 4093 5 Size: 47 4041 2081 Fragments arranged by size: 4041 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6169 StyI C'CwwG_G Cuts at: 513 1065 3229 3608 513 Size: 552 2164 379 3074 Fragments arranged by size: 3074 2164 552 379 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4196 4213 4366 4551 4996 325 Size: 1255 2616 17 153 185 445 1498 Fragments arranged by size: 2616 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2283 3173 4415 Size: 274 80 33 51 1443 306 890 1242 Cuts at: 4415 6127 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1242 890 306 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3122 3154 6129 6161 3122 Size: 32 2975 32 3130 Fragments arranged by size: 3130 2975 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6169 XbaI T'CTAG_A Cuts at: 3505 3505 Size: 6169 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2839 2866 2839 Size: 27 6142 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6169 XmaI C'CCGG_G Cuts at: 1870 2015 2261 2718 3150 3494 1870 Size: 145 246 457 432 344 4545 Fragments arranged by size: 4545 457 432 344 246 145 XmnI GAAnn'nnTTC Cuts at: 2956 4298 2956 Size: 1342 4827 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3977 276 Size: 53 83 186 936 2443 2468 Fragments arranged by size: 2468 2443 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SfiI SrfI SwaI