################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI AlwNI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BbeI BbrPI BcgI BclI BcnI BfrI BglI BglII BlnI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspLU11I BspMI BsrBI BssHII Bst1107I BstAPI BstBI BstZ17I CfoI ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI MamI MfeI MluI MroI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccI 1261 GTCTAC GT^[AC][GT]AC AcsI 640 GAATTC [GA]^AATT[CT] Alw44I 1058 GTGCAC G^TGCAC AocI 763 CCTCAGG CC^T[ACGT]AGG ApaLI 1058 GTGCAC G^TGCAC ApoI 640 GAATTC [GA]^AATT[CT] BalI 119 TGGCCA TGG^CCA BamHI 1047 GGATCC G^GATCC BanI 571 GGTGCC G^G[CT][GA]CC BbsI 623 GAAGAC GAAGAC(2/6) BbvCI 225 CCTCAGC CCTCAGC(-5/-2) BciVI 1174 GTATCC GTATCC(6/5) BfaI 46 CTAG C^TAG BlpI 180 GCTCAGC GC^T[ACGT]AGC BpmI 613 CTGGAG CTGGAG(16/14) Bpu1102I 180 GCTCAGC GC^T[ACGT]AGC BpuAI 623 GAAGAC GAAGAC(2/6) BsaAI 832 TACGTA [CT]AC^GT[GA] BsaI 306 GAGACC GGTCTC(1/5) BsmAI 306 GAGAC GTCTC(1/5) BsmFI 1231 GGGAC GGGAC(10/14) BsmI 743 GCATTC GAATGC(1/-1) BspHI 332 TCATGA T^CATGA BsrFI 284 GCCGGT [GA]^CCGG[CT] BssSI 340 CTCGTG CACGAG(-5/-1) BstEII 580 GGTTACC G^GT[ACGT]ACC BstPI 580 GGTTACC G^GT[ACGT]ACC BstXI 969 CCACCTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1047 GGATCC [GA]^GATC[CT] Bsu36I 763 CCTCAGG CC^T[ACGT]AGG CelII 180 GCTCAGC GC^T[ACGT]AGC Cfr10I 284 GCCGGT [GA]^CCGG[CT] DraIII 678 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 903 GACATTAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 763 CCTCAGG CC^T[ACGT]AGG EcoO65I 580 GGTTACC G^GT[ACGT]ACC EcoRI 640 GAATTC G^AATTC EcoT14I 400 CCAAGG C^C[AT][AT]GG FauI 1043 GCGGG CCCGC(4/6) KspI 1041 CCGCGG CCGC^GG MaeI 46 CTAG C^TAG MaeII 833 ACGT A^CGT MflI 1047 GGATCC [GA]^GATC[CT] MluNI 119 TGGCCA TGG^CCA MscI 119 TGGCCA TGG^CCA MspA1I 1041 CCGCGG C[AC]G^C[GT]G PpuMI 767 AGGACCC [GA]G^G[AT]CC[CT] PshAI 168 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 250 CTGCAG CTGCA^G RcaI 332 TCATGA T^CATGA SacII 1041 CCGCGG CCGC^GG SapI 1248 GCTCTTC GCTCTTC(1/4) SnaBI 832 TACGTA TAC^GTA StyI 400 CCAAGG C^C[AT][AT]GG TfiI 242 GATTC G^A[AT]TC Tsp509I 641 AATT ^AATT XhoII 1047 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### ApaI 481 GGGCCC GGGCC^C 605 GGGCCC BseRI 941 CTCCTC GAGGAG(10/8) 1094 GAGGAG BsgI 702 GTGCAG GTGCAG(16/14) 1281 GTGCAG Bsp1407I 206 TGTACA T^GTACA 1313 TGTACA BsrDI 850 GCAATG GCAATG(2/0) 898 GCAATG BsrGI 206 TGTACA T^GTACA 1313 TGTACA DpnII 266 GATC ^GATC 1048 GATC DraII 533 AGGGCCT [GA]G^G[ACGT]CC[CT] 767 AGGACCC DsaI 257 CCGTGG C^C[GA][CT]GG 1041 CCGCGG EcoO109I 533 AGGGCCT [GA]G^G[ACGT]CC[CT] 767 AGGACCC HapII 285 CCGG C^CGG 303 CCGG HpaII 285 CCGG C^CGG 303 CCGG MboI 266 GATC ^GATC 1048 GATC MslI 117 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 541 CACTACCATG MspI 285 CCGG C^CGG 303 CCGG NdeII 266 GATC ^GATC 1048 GATC NspI 935 ACATGC [GA]CATG^[CT] 1001 ACATGC Sau3AI 266 GATC ^GATC 1048 GATC SspBI 206 TGTACA T^GTACA 1313 TGTACA TaqI 812 TCGA T^CGA 1100 TCGA TthHB8I 812 TCGA T^CGA 1100 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI AvaII BanII BbvI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I Cfr13I DdeI EaeI EarI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeIII MboII MnlI MseI MvaI MvnI MwoI NlaIII NlaIV PleI RsaI Sau96I ScrFI SfaNI SfcI Tru9I TseI Tsp45I TspRI