(Circular) (Six-base) MAPSORT of: pFN21AE4444.seq Check: 5570 from: 1 to: 6085 pFN21AE4444 6085 bp With 182 enzymes: SgfI * March 28, 2012 14:02 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3895 278 Size: 53 83 186 936 2359 2468 Fragments arranged by size: 2468 2359 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3267 3428 1937 Size: 1330 161 4594 Fragments arranged by size: 4594 1330 161 Acc65I G'GTAC_C Cuts at: 3406 3406 Size: 6085 AclI AA'CG_TT Cuts at: 1563 4212 4585 1563 Size: 2649 373 3063 Fragments arranged by size: 3063 2649 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2739 4148 5532 5800 1632 Size: 1107 1409 1384 268 1917 Fragments arranged by size: 1917 1409 1384 1107 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5888 AflIII A'CryG_T Cuts at: 1203 4984 5961 1203 Size: 3781 977 1327 Fragments arranged by size: 3781 1327 977 AgeI A'CCGG_T Cuts at: 4916 4916 Size: 6085 AhdI GACnn_n'nnGTC Cuts at: 4814 4814 Size: 6085 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6085 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6085 AlwNI CAG_nnn'CTG Cuts at: 3828 5400 3828 Size: 1572 4513 ApaI G_GGCC'C Cuts at: 2490 2614 2490 Size: 124 5961 ApaLI G'TGCA_C Cuts at: 3063 4142 5298 3063 Size: 1079 1156 3850 Fragments arranged by size: 3850 1156 1079 ApoI r'AATT_y Cuts at: 1493 2645 3394 3620 3772 5881 1493 Size: 1152 749 226 152 2109 1697 Fragments arranged by size: 2109 1697 1152 749 226 152 AseI AT'TA_AT Cuts at: 160 1032 4639 160 Size: 872 3607 1606 Fragments arranged by size: 3607 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6085 AvaI C'yCGr_G Cuts at: 1870 1943 3410 1870 Size: 73 1467 4545 Fragments arranged by size: 4545 1467 73 BamHI G'GATC_C Cuts at: 3052 3415 3782 3052 Size: 363 367 5355 Fragments arranged by size: 5355 367 363 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2576 3406 4861 618 Size: 325 217 627 789 830 1455 1842 Fragments arranged by size: 1842 1455 830 789 627 325 217 BanII G_rGCy'C Cuts at: 1391 2490 2614 3255 3404 1391 Size: 1099 124 641 149 4072 Fragments arranged by size: 4072 1099 641 149 124 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2635 928 Size: 971 736 4378 Fragments arranged by size: 4378 971 736 BbvCI CC'TCA_GC Cuts at: 2231 2231 Size: 6085 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4276 4310 1903 Size: 34 2339 34 3678 Fragments arranged by size: 3678 2339 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3190 3977 5187 3190 Size: 787 1210 4088 Fragments arranged by size: 4088 1210 787 BclI T'GATC_A Cuts at: 1548 1548 Size: 6085 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4696 136 Size: 107 122 71 631 605 3024 1525 Fragments arranged by size: 3024 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6080 6080 Size: 6085 BlpI GC'TnA_GC Cuts at: 2186 3502 2186 Size: 1316 4769 Bme1580I G_kGCm'C Cuts at: 1790 2490 2581 2614 3067 3330 4146 5302 1790 Size: 700 91 33 453 263 816 1156 2573 Fragments arranged by size: 2573 1156 816 700 453 263 91 33 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6085 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2171 2617 3816 Size: 416 53 83 399 24 754 446 1199 Cuts at: 3816 4774 442 Size: 958 1753 Fragments arranged by size: 1753 1199 958 754 446 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6085 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2639 4745 1239 Size: 1400 2106 2579 Fragments arranged by size: 2579 2106 1400 Bpu10I CC'TnA_GC Cuts at: 2231 2231 Size: 6085 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4210 5075 5373 4210 Size: 865 298 4922 Fragments arranged by size: 4922 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2305 4748 882 Size: 617 806 2443 2219 Fragments arranged by size: 2443 2219 806 617 BsaAI yAC'GTr Cuts at: 493 1204 2839 3876 493 Size: 711 1635 1037 2702 Fragments arranged by size: 2702 1635 1037 711 BsaBI GATnn'nnATC Cuts at: 6079 6079 Size: 6085 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3892 4274 4909 275 Size: 53 83 186 936 2359 382 635 1451 Fragments arranged by size: 2359 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4518 4916 5190 5337 5748 4518 Size: 398 274 147 411 4855 Fragments arranged by size: 4855 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2150 2180 3093 3123 1178 Size: 30 165 30 747 30 913 30 4140 Fragments arranged by size: 4140 913 747 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2937 3114 1186 Size: 1751 177 4157 Fragments arranged by size: 4157 1751 177 BseYI C'CCAG_C Cuts at: 2201 2623 5288 5784 2201 Size: 422 2665 496 2502 Fragments arranged by size: 2665 2502 496 422 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2728 3307 1163 Size: 573 992 579 3941 Fragments arranged by size: 3941 992 579 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3535 4296 5324 1540 Size: 275 186 1534 761 1028 2301 Fragments arranged by size: 2301 1534 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2682 2932 3067 4146 4231 5302 2682 Size: 250 135 1079 85 1071 3465 Fragments arranged by size: 3465 1079 1071 250 135 85 BsmI GAATG_Cn' Cuts at: 2748 3596 3689 2748 Size: 848 93 5144 Fragments arranged by size: 5144 848 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2490 2581 2614 2682 2932 3067 3255 Size: 399 700 91 33 68 250 135 188 Cuts at: 3255 3330 3404 4146 4231 5302 1391 Size: 75 74 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 700 399 250 188 135 91 85 75 74 68 33 BspEI T'CCGG_A Cuts at: 5748 5748 Size: 6085 BspHI T'CATG_A Cuts at: 2337 3974 5704 2337 Size: 1637 1730 2718 Fragments arranged by size: 2718 1730 1637 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3440 844 Size: 2596 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3972 1394 Size: 429 2149 3507 Fragments arranged by size: 3507 2149 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2862 2910 4580 4754 65 Size: 1183 1614 48 1670 174 1396 Fragments arranged by size: 1670 1614 1396 1183 174 48 BsrFI r'CCGG_y Cuts at: 1700 1810 2289 4729 4916 1700 Size: 110 479 2440 187 2869 Fragments arranged by size: 2869 2440 479 187 110 BsrGI T'GTAC_A Cuts at: 96 2211 3318 96 Size: 2115 1107 2863 Fragments arranged by size: 2863 2115 1107 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6085 BssSI C'ACGA_G Cuts at: 2345 4145 5157 2345 Size: 1800 1012 3273 Fragments arranged by size: 3273 1800 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6085 BstBI TT'CG_AA Cuts at: 4889 4889 Size: 6085 BstEII G'GTnAC_C Cuts at: 1184 2585 1184 Size: 1401 4684 BstXI CCAn_nnnn'nTGG Cuts at: 2981 2981 Size: 6085 BstYI r'GATC_y Cuts at: 1972 3052 3415 3782 4167 4184 5636 5722 5734 Size: 1080 363 367 385 17 1452 86 12 Cuts at: 5734 6080 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1080 385 367 363 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2769 2769 Size: 6085 BtgI C'CryG_G Cuts at: 513 1065 2004 2262 3046 513 Size: 552 939 258 784 3552 Fragments arranged by size: 3552 939 784 552 258 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5346 Fragments arranged by size: 5346 683 56 BtsI GCAGTG_nn' Cuts at: 2284 2918 3601 4395 4415 2284 Size: 634 683 794 20 3954 Fragments arranged by size: 3954 794 683 634 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6085 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6050 DraI TTT'AAA Cuts at: 3389 3389 Size: 6085 DraIII CAC_nnn'GTG Cuts at: 2688 2688 Size: 6085 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2914 5092 809 Size: 507 1598 2178 1802 Fragments arranged by size: 2178 1802 1598 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2029 2124 2274 Size: 54 1425 50 275 18 199 95 150 Cuts at: 2274 3532 4421 6050 8 Size: 1258 889 1629 43 Fragments arranged by size: 1629 1425 1258 889 275 199 150 95 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3532 1537 Size: 275 1720 4090 Fragments arranged by size: 4090 1720 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2131 2564 3260 4015 1353 Size: 778 433 696 755 3423 Fragments arranged by size: 3423 778 755 696 433 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4672 5046 5192 387 Size: 1064 138 3083 374 146 1280 Fragments arranged by size: 3083 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2639 2739 4148 4745 5532 5800 1239 Size: 393 1007 100 1409 597 787 268 1524 Fragments arranged by size: 1524 1409 1007 787 597 393 268 100 EcoO109I rG'GnC_Cy Cuts at: 2539 2773 2539 Size: 234 5851 EcoRI G'AATT_C Cuts at: 2645 3394 3772 2645 Size: 749 378 4958 Fragments arranged by size: 4958 749 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2744 2744 Size: 6085 FspI TGC'GCA Cuts at: 4591 4591 Size: 6085 HaeII r_GCGC'y Cuts at: 1678 5232 1678 Size: 3554 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2304 2336 2501 2533 4726 Size: 32 135 32 732 32 165 32 2193 Cuts at: 4726 4758 4800 4832 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2193 732 165 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3429 3675 669 Size: 988 281 1491 246 3079 Fragments arranged by size: 3079 1491 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6085 HpaI GTT'AAC Cuts at: 3675 3675 Size: 6085 KpnI G_GTAC'C Cuts at: 3410 3410 Size: 6085 MfeI C'AATT_G Cuts at: 3684 3684 Size: 6085 MluI A'CGCG_T Cuts at: 5961 5961 Size: 6085 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2248 3027 3091 5199 5383 1305 Size: 943 779 64 2108 184 2007 Fragments arranged by size: 2108 2007 943 779 184 64 MscI TGG'CCA Cuts at: 10 64 1489 1832 2126 10 Size: 54 1425 343 294 3969 Fragments arranged by size: 3969 1425 343 294 54 MslI CAynn'nnrTG Cuts at: 518 1166 2126 2550 4043 4402 4561 518 Size: 648 960 424 1493 359 159 2042 Fragments arranged by size: 2042 1493 960 648 424 359 159 MspA1I CmG'CkG Cuts at: 1606 3048 3501 4178 5326 5571 1606 Size: 1442 453 677 1148 245 2120 Fragments arranged by size: 2120 1442 1148 677 453 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4594 Fragments arranged by size: 4594 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6085 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6085 NotI GC'GGCC_GC Cuts at: 3532 3532 Size: 6085 NspI r_CATG'y Cuts at: 1132 1229 2944 3010 3443 4988 1132 Size: 97 1715 66 433 1545 2229 Fragments arranged by size: 2229 1715 1545 433 97 66 PasI CC'CwG_GG Cuts at: 1348 2329 2581 1348 Size: 981 252 4852 Fragments arranged by size: 4852 981 252 PciI A'CATG_T Cuts at: 4984 4984 Size: 6085 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6085 PmeI GTTT'AAAC Cuts at: 3389 3389 Size: 6085 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4154 4186 5667 5699 4154 Size: 32 1481 32 4540 Fragments arranged by size: 4540 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2773 2773 Size: 6085 PshAI GACnn'nnGTC Cuts at: 1613 2177 1613 Size: 564 5521 PsiI TTA'TAA Cuts at: 3655 3655 Size: 6085 PspOMI G'GGCC_C Cuts at: 2486 2610 2486 Size: 124 5961 PstI C_TGCA'G Cuts at: 830 2259 3437 830 Size: 1429 1178 3478 Fragments arranged by size: 3478 1429 1178 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6085 SacII CC_GC'GG Cuts at: 3049 3049 Size: 6085 SalI G'TCGA_C Cuts at: 1936 3427 1936 Size: 1491 4594 SapI GCTCTTCn'nnn_ Cuts at: 3260 3260 Size: 6085 SbfI CC_TGCA'GG Cuts at: 3437 3437 Size: 6085 ScaI AGT'ACT Cuts at: 4333 6045 4333 Size: 1712 4373 SfcI C'TryA_G Cuts at: 826 1045 2255 2601 3058 3433 4568 5249 5440 Size: 219 1210 346 457 375 1135 681 191 Cuts at: 5440 826 Size: 1471 Fragments arranged by size: 1471 1210 1135 681 457 375 346 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6085 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6085 SmaI CCC'GGG Cuts at: 1872 3412 1872 Size: 1540 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4189 5090 5352 820 Size: 197 926 2246 901 262 1553 Fragments arranged by size: 2246 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2839 3876 493 Size: 2346 1037 2702 Fragments arranged by size: 2702 2346 1037 SpeI A'CTAG_T Cuts at: 152 152 Size: 6085 SphI G_CATG'C Cuts at: 1132 3443 1132 Size: 2311 3774 SspI AAT'ATT Cuts at: 5 52 4009 5 Size: 47 3957 2081 Fragments arranged by size: 3957 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6085 StyI C'CwwG_G Cuts at: 513 1065 2004 2405 3524 513 Size: 552 939 401 1119 3074 Fragments arranged by size: 3074 1119 939 552 401 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2585 4112 4129 4282 4467 4912 325 Size: 1255 1005 1527 17 153 185 445 1498 Fragments arranged by size: 1527 1498 1255 1005 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2211 3318 4331 Size: 274 80 33 51 1443 234 1107 1013 Cuts at: 4331 6043 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1107 1013 274 234 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2276 2308 6045 6077 2276 Size: 32 3737 32 2284 Fragments arranged by size: 3737 2284 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6085 XbaI T'CTAG_A Cuts at: 3421 3421 Size: 6085 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6085 XmaI C'CCGG_G Cuts at: 1870 3410 1870 Size: 1540 4545 XmnI GAAnn'nnTTC Cuts at: 4214 4214 Size: 6085 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3893 276 Size: 53 83 186 936 2359 2468 Fragments arranged by size: 2468 2359 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI