(Linear) (Six-base) MAPSORT of: KIEE4444.seq Check: 8563 from: 1 to: 1380 KIEE4444.orf With 182 enzymes: SgfI * March 22, 2012 18:03 .. AccI GT'mk_AC Cuts at: 0 1262 1380 Size: 1262 118 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 734 1380 Size: 734 646 ApaI G_GGCC'C Cuts at: 0 485 609 1380 Size: 485 124 771 Fragments arranged by size: 771 485 124 ApaLI G'TGCA_C Cuts at: 0 1058 1380 Size: 1058 322 ApoI r'AATT_y Cuts at: 0 640 1380 Size: 640 740 BamHI G'GATC_C Cuts at: 0 1047 1380 Size: 1047 333 BanI G'GyrC_C Cuts at: 0 571 1380 Size: 571 809 BanII G_rGCy'C Cuts at: 0 485 609 1250 1380 Size: 485 124 641 130 Fragments arranged by size: 641 485 130 124 BbsI GAAGACnn'nnnn_ Cuts at: 0 630 1380 Size: 630 750 BbvCI CC'TCA_GC Cuts at: 0 226 1380 Size: 226 1154 BciVI GTATCCnnnnn_n' Cuts at: 0 1185 1380 Size: 1185 195 BlpI GC'TnA_GC Cuts at: 0 181 1380 Size: 181 1199 Bme1580I G_kGCm'C Cuts at: 0 485 576 609 1062 1325 1380 Size: 485 91 33 453 263 55 Fragments arranged by size: 485 453 263 91 55 33 BmrI ACTGGGnnnn_n' Cuts at: 0 166 612 1380 Size: 166 446 768 Fragments arranged by size: 768 446 166 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 634 1380 Size: 634 746 Bpu10I CC'TnA_GC Cuts at: 0 226 1380 Size: 226 1154 BsaI GGTCTCn'nnnn_ Cuts at: 0 300 1380 Size: 300 1080 BsaAI yAC'GTr Cuts at: 0 834 1380 Size: 834 546 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 145 175 1088 1118 1380 Size: 145 30 913 30 262 Fragments arranged by size: 913 262 145 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 932 1109 1380 Size: 932 177 271 Fragments arranged by size: 932 271 177 BseYI C'CCAG_C Cuts at: 0 196 618 1380 Size: 196 422 762 Fragments arranged by size: 762 422 196 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 723 1302 1380 Size: 723 579 78 Fragments arranged by size: 723 579 78 BsiHKAI G_wGCw'C Cuts at: 0 677 927 1062 1380 Size: 677 250 135 318 Fragments arranged by size: 677 318 250 135 BsmI GAATG_Cn' Cuts at: 0 743 1380 Size: 743 637 Bsp1286I G_dGCh'C Cuts at: 0 485 576 609 677 927 1062 1250 1325 Size: 485 91 33 68 250 135 188 75 Cuts at: 1325 1380 Size: 55 Fragments arranged by size: 485 250 188 135 91 75 68 55 33 BspHI T'CATG_A Cuts at: 0 332 1380 Size: 332 1048 BsrDI GCAATG_nn' Cuts at: 0 857 905 1380 Size: 857 48 475 Fragments arranged by size: 857 475 48 BsrFI r'CCGG_y Cuts at: 0 284 1380 Size: 284 1096 BsrGI T'GTAC_A Cuts at: 0 206 1313 1380 Size: 206 1107 67 Fragments arranged by size: 1107 206 67 BssSI C'ACGA_G Cuts at: 0 340 1380 Size: 340 1040 BstEII G'GTnAC_C Cuts at: 0 580 1380 Size: 580 800 BstXI CCAn_nnnn'nTGG Cuts at: 0 976 1380 Size: 976 404 BstYI r'GATC_y Cuts at: 0 1047 1380 Size: 1047 333 Bsu36I CC'TnA_GG Cuts at: 0 764 1380 Size: 764 616 BtgI C'CryG_G Cuts at: 0 257 1041 1380 Size: 257 784 339 Fragments arranged by size: 784 339 257 BtsI GCAGTG_nn' Cuts at: 0 279 913 1380 Size: 279 634 467 Fragments arranged by size: 634 467 279 DraIII CAC_nnn'GTG Cuts at: 0 683 1380 Size: 683 697 DrdI GACnn_nn'nnGTC Cuts at: 0 909 1380 Size: 909 471 EaeI y'GGCC_r Cuts at: 0 24 119 269 1380 Size: 24 95 150 1111 Fragments arranged by size: 1111 150 95 24 EarI CTCTTCn'nnn_ Cuts at: 0 126 559 1255 1380 Size: 126 433 696 125 Fragments arranged by size: 696 433 126 125 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 634 734 1380 Size: 634 100 646 Fragments arranged by size: 646 634 100 EcoO109I rG'GnC_Cy Cuts at: 0 534 768 1380 Size: 534 234 612 Fragments arranged by size: 612 534 234 EcoRI G'AATT_C Cuts at: 0 640 1380 Size: 640 740 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 739 1380 Size: 739 641 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 299 331 496 528 1380 Size: 299 32 165 32 852 Fragments arranged by size: 852 299 165 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 243 1022 1086 1380 Size: 243 779 64 294 Fragments arranged by size: 779 294 243 64 MscI TGG'CCA Cuts at: 0 121 1380 Size: 121 1259 MslI CAynn'nnrTG Cuts at: 0 121 545 1380 Size: 121 424 835 Fragments arranged by size: 835 424 121 MspA1I CmG'CkG Cuts at: 0 1043 1380 Size: 1043 337 NspI r_CATG'y Cuts at: 0 939 1005 1380 Size: 939 66 375 Fragments arranged by size: 939 375 66 PasI CC'CwG_GG Cuts at: 0 324 576 1380 Size: 324 252 804 Fragments arranged by size: 804 324 252 PpuMI rG'GwC_Cy Cuts at: 0 768 1380 Size: 768 612 PshAI GACnn'nnGTC Cuts at: 0 172 1380 Size: 172 1208 PspOMI G'GGCC_C Cuts at: 0 481 605 1380 Size: 481 124 775 Fragments arranged by size: 775 481 124 PstI C_TGCA'G Cuts at: 0 254 1380 Size: 254 1126 SacII CC_GC'GG Cuts at: 0 1044 1380 Size: 1044 336 SapI GCTCTTCn'nnn_ Cuts at: 0 1255 1380 Size: 1255 125 SfcI C'TryA_G Cuts at: 0 250 596 1053 1380 Size: 250 346 457 327 Fragments arranged by size: 457 346 327 250 SnaBI TAC'GTA Cuts at: 0 834 1380 Size: 834 546 StyI C'CwwG_G Cuts at: 0 400 1380 Size: 400 980 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 580 1380 Size: 580 800 TatI w'GTAC_w Cuts at: 0 206 1313 1380 Size: 206 1107 67 Fragments arranged by size: 1107 206 67 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 271 303 1380 Size: 271 32 1077 Fragments arranged by size: 1077 271 32 Enzymes that do cut: AccI AcuI ApaI ApaLI ApoI BamHI BanI BanII BbsI BbvCI BciVI BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrFI BsrGI BssSI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EarI Eco57MI EcoO109I EcoRI FalI Hin4I MmeI MscI MslI MspA1I NspI PasI PpuMI PshAI PspOMI PstI SacII SapI SfcI SnaBI StyI TaqII TatI TstI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI AscI AseI AsiSI AvaI AvrII BaeI BbeI BcgI BclI BfrBI BglI BglII BmgBI BmtI BplI BpuEI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BssHII BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraI EagI EciI EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI