Restriction map for
pFN21AE1499
(Linear) (Six-base) MAPSORT of: KIEE1499.seq Check: 4876 from: 1 to: 9291
KIEE1499.orf
With 182 enzymes: SgfI *
August 2, 2010 17:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1719 2154 2741 2886 9291
Size: 1719 435 587 145 6405
Fragments arranged by size:
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AatII G_ACGT'C
Cuts at: 0 2534 3248 4933 9291
Size: 2534 714 1685 4358
Fragments arranged by size:
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AccI GT'mk_AC
Cuts at: 0 1412 2615 3513 7715 9291
Size: 1412 1203 898 4202 1576
Fragments arranged by size:
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Acc65I G'GTAC_C
Cuts at: 0 8711 9291
Size: 8711 580
AclI AA'CG_TT
Cuts at: 0 265 9291
Size: 265 9026
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 694 808 832 1021 1123 3232 3731
Size: 445 249 114 24 189 102 2109 499
Cuts at: 3731 4676 4810 4822 6352 8251 8684 9291
Size: 945 134 12 1530 1899 433 607
Fragments arranged by size:
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249 189 134 114 102 24 12
AfeI AGC'GCT
Cuts at: 0 2134 9291
Size: 2134 7157
AflII C'TTAA_G
Cuts at: 0 4711 9291
Size: 4711 4580
AflIII A'CryG_T
Cuts at: 0 1178 1334 2012 2090 4638 6167 7760 8083
Size: 1178 156 678 78 2548 1529 1593 323
Cuts at: 8083 8270 9185 9291
Size: 187 915 106
Fragments arranged by size:
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156 106 78
AhdI GACnn_n'nnGTC
Cuts at: 0 2406 7095 9291
Size: 2406 4689 2196
Fragments arranged by size:
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AleI CACnn'nnGTG
Cuts at: 0 2035 7690 7841 9291
Size: 2035 5655 151 1450
Fragments arranged by size:
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AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3653 5332 7450 9291
Size: 3653 1679 2118 1841
Fragments arranged by size:
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AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 7715 7747 8804 8836 8890 8922 9291
Size: 7715 32 1057 32 54 32 369
Fragments arranged by size:
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AlwNI CAG_nnn'CTG
Cuts at: 0 843 972 1579 1695 2289 2640 2757 6977
Size: 843 129 607 116 594 351 117 4220
Cuts at: 6977 8856 8881 9147 9291
Size: 1879 25 266 144
Fragments arranged by size:
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129 117 116 25
ApaI G_GGCC'C
Cuts at: 0 3145 5280 5296 6939 7121 8161 9291
Size: 3145 2135 16 1643 182 1040 1130
Fragments arranged by size:
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ApaLI G'TGCA_C
Cuts at: 0 1600 9291
Size: 1600 7691
ApoI r'AATT_y
Cuts at: 0 526 532 4045 4660 6703 6803 7736 8056
Size: 526 6 3513 615 2043 100 933 320
Cuts at: 8056 8869 9291
Size: 813 422
Fragments arranged by size:
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100 6
AvaI C'yCGr_G
Cuts at: 0 75 2404 2627 3079 5547 7071 7114 7147
Size: 75 2329 223 452 2468 1524 43 33
Cuts at: 7147 7275 7346 7506 8893 9291
Size: 128 71 160 1387 398
Fragments arranged by size:
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128 75 71 43 33
AvrII C'CTAG_G
Cuts at: 0 8328 9291
Size: 8328 963
BamHI G'GATC_C
Cuts at: 0 2765 3458 6133 6785 7019 9291
Size: 2765 693 2675 652 234 2272
Fragments arranged by size:
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BanI G'GyrC_C
Cuts at: 0 91 6985 7261 7324 8711 9291
Size: 91 6894 276 63 1387 580
Fragments arranged by size:
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BanII G_rGCy'C
Cuts at: 0 759 2321 2351 3145 3508 3533 3899 4800
Size: 759 1562 30 794 363 25 366 901
Cuts at: 4800 4811 5280 5287 5296 5726 6939 7121 7252
Size: 11 469 7 9 430 1213 182 131
Cuts at: 7252 7288 7415 7450 7462 8161 9291
Size: 36 127 35 12 699 1130
Fragments arranged by size:
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430 366 363 182 131 127 36 35
30 25 12 11 9 7
BbeI G_GCGC'C
Cuts at: 0 6989 7265 9291
Size: 6989 276 2026
Fragments arranged by size:
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BbsI GAAGACnn'nnnn_
Cuts at: 0 668 1964 3119 3240 5913 9291
Size: 668 1296 1155 121 2673 3378
Fragments arranged by size:
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BbvCI CC'TCA_GC
Cuts at: 0 1024 4408 7805 9291
Size: 1024 3384 3397 1486
Fragments arranged by size:
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BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2077 2111 3060 3094 5443 5477 9291
Size: 2077 34 949 34 2349 34 3814
Fragments arranged by size:
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BciVI GTATCCnnnnn_n'
Cuts at: 0 848 3811 3939 6363 7557 8913 9291
Size: 848 2963 128 2424 1194 1356 378
Fragments arranged by size:
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BclI T'GATC_A
Cuts at: 0 470 2180 3176 6290 8439 9291
Size: 470 1710 996 3114 2149 852
Fragments arranged by size:
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BglI GCCn_nnn'nGGC
Cuts at: 0 58 67 3445 3640 6962 7150 7173 7490
Size: 58 9 3378 195 3322 188 23 317
Cuts at: 7490 9291
Size: 1801
Fragments arranged by size:
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9
BglII A'GATC_T
Cuts at: 0 2457 2550 6282 9291
Size: 2457 93 3732 3009
Fragments arranged by size:
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BlpI GC'TnA_GC
Cuts at: 0 3893 4372 5350 8399 9291
Size: 3893 479 978 3049 892
Fragments arranged by size:
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Bme1580I G_kGCm'C
Cuts at: 0 1604 3145 5280 5296 5375 5603 6939 7121
Size: 1604 1541 2135 16 79 228 1336 182
Cuts at: 7121 7327 7798 8161 9291
Size: 206 471 363 1130
Fragments arranged by size:
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206 182 79 16
BmgBI CAC'GTC
Cuts at: 0 2054 2091 4322 6732 9188 9291
Size: 2054 37 2231 2410 2456 103
Fragments arranged by size:
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BmrI ACTGGGnnnn_n'
Cuts at: 0 1919 6935 8866 9291
Size: 1919 5016 1931 425
Fragments arranged by size:
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BmtI G_CTAG'C
Cuts at: 0 7319 9291
Size: 7319 1972
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 721 1297 1519 1672 2080 2214 2365 2467
Size: 721 576 222 153 408 134 151 102
Cuts at: 2467 2665 2728 2821 3211 3492 3760 4381 5077
Size: 198 63 93 390 281 268 621 696
Cuts at: 5077 5145 5202 5242 5626 5788 6694 7264 7368
Size: 68 57 40 384 162 906 570 104
Cuts at: 7368 7476 7510 8524 9118 9291
Size: 108 34 1014 594 173
Fragments arranged by size:
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408 390 384 281 268 222 198 173
162 153 151 134 108 104 102 93
68 63 57 40 34
Bpu10I CC'TnA_GC
Cuts at: 0 894 1024 2212 4408 5515 7805 9291
Size: 894 130 1188 2196 1107 2290 1486
Fragments arranged by size:
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BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2986 3004 6328 7861 9291
Size: 2986 18 3324 1533 1430
Fragments arranged by size:
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BsaI GGTCTCn'nnnn_
Cuts at: 0 555 3920 4643 6733 7062 8092 8572 8989
Size: 555 3365 723 2090 329 1030 480 417
Cuts at: 8989 9291
Size: 302
Fragments arranged by size:
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302
BsaAI yAC'GTr
Cuts at: 0 3165 3420 3950 5376 7583 8084 8273 8924
Size: 3165 255 530 1426 2207 501 189 651
Cuts at: 8924 8949 9291
Size: 25 342
Fragments arranged by size:
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189 25
BsaBI GATnn'nnATC
Cuts at: 0 643 4915 9291
Size: 643 4272 4376
Fragments arranged by size:
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BsaHI Gr'CG_yC
Cuts at: 0 2381 2531 2977 3169 3245 4930 5228 5432
Size: 2381 150 446 192 76 1685 298 204
Cuts at: 5432 5594 6986 7193 7262 7292 9176 9291
Size: 162 1392 207 69 30 1884 115
Fragments arranged by size:
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192 162 150 115 76 69 30
BsaWI w'CCGG_w
Cuts at: 0 1961 8708 9291
Size: 1961 6747 583
Fragments arranged by size:
6747 1961 583
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1270 1300 1324 1354 2239 2269 2584 2614
Size: 1270 30 24 30 885 30 315 30
Cuts at: 2614 5194 5224 7504 7534 7715 7745 8185 8215
Size: 2580 30 2280 30 181 30 440 30
Cuts at: 8215 8362 8392 8804 8834 8890 8920 9064 9094
Size: 147 30 412 30 56 30 144 30
Cuts at: 9094 9291
Size: 197
Fragments arranged by size:
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181 147 144 56 30 30 30 30
30 30 30 30 30 30 30 30
24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 124 364 2077 2329 3447 3562 5506 5532
Size: 124 240 1713 252 1118 115 1944 26
Cuts at: 5532 5535 5596 5629 5847 7050 7578 8554 9291
Size: 3 61 33 218 1203 528 976 737
Fragments arranged by size:
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240 218 124 115 61 33 26 3
BseYI C'CCAG_C
Cuts at: 0 343 2757 3138 5112 5779 6970 6991 7025
Size: 343 2414 381 1974 667 1191 21 34
Cuts at: 7025 7159 7168 7423 9291
Size: 134 9 255 1868
Fragments arranged by size:
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134 34 21 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 937 958 1618 2137 2740 2920 3247 5735
Size: 937 21 660 519 603 180 327 2488
Cuts at: 5735 6322 7630 7865 8701 9291
Size: 587 1308 235 836 590
Fragments arranged by size:
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519 327 235 180 21
BsiEI CG_ry'CG
Cuts at: 0 5065 5305 5489 9291
Size: 5065 240 184 3802
Fragments arranged by size:
5065 3802 240 184
BsiHKAI G_wGCw'C
Cuts at: 0 1324 1604 2321 2351 2849 3533 3545 3899
Size: 1324 280 717 30 498 684 12 354
Cuts at: 3899 5287 5726 6209 7462 7582 7588 8942 9291
Size: 1388 439 483 1253 120 6 1354 349
Fragments arranged by size:
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439 354 349 280 120 30 12 6
BsmI GAATG_Cn'
Cuts at: 0 3205 3243 6753 9291
Size: 3205 38 3510 2538
Fragments arranged by size:
3510 3205 2538 38
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2266 2984 3148 3387 4880 4937 5220 5476
Size: 2266 718 164 239 1493 57 283 256
Cuts at: 5476 9291
Size: 3815
Fragments arranged by size:
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57
Bsp1286I G_dGCh'C
Cuts at: 0 759 1324 1604 2321 2351 2849 3145 3508
Size: 759 565 280 717 30 498 296 363
Cuts at: 3508 3533 3545 3899 4800 4811 5280 5287 5296
Size: 25 12 354 901 11 469 7 9
Cuts at: 5296 5375 5603 5726 6209 6939 7121 7252 7288
Size: 79 228 123 483 730 182 131 36
Cuts at: 7288 7327 7415 7450 7462 7582 7588 7798 8161
Size: 39 88 35 12 120 6 210 363
Cuts at: 8161 8942 9291
Size: 781 349
Fragments arranged by size:
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469 363 363 354 349 296 280 228
210 182 131 123 120 88 79 39
36 35 30 25 12 12 11 9
7 6
BspHI T'CATG_A
Cuts at: 0 285 1827 2655 3837 6311 9291
Size: 285 1542 828 1182 2474 2980
Fragments arranged by size:
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BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 917 1719 2154 2648 2741 2886 3054 3269
Size: 917 802 435 494 93 145 168 215
Cuts at: 3269 8723 9007 9291
Size: 5454 284 284
Fragments arranged by size:
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168 145 93
BsrBI CCG'CTC
Cuts at: 0 5 1317 4776 4831 5061 5108 7380 9213
Size: 5 1312 3459 55 230 47 2272 1833
Cuts at: 9213 9291
Size: 78
Fragments arranged by size:
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5
BsrDI GCAATG_nn'
Cuts at: 0 681 906 1813 5229 9291
Size: 681 225 907 3416 4062
Fragments arranged by size:
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BsrFI r'CCGG_y
Cuts at: 0 94 947 5434 7007 7153 9154 9291
Size: 94 853 4487 1573 146 2001 137
Fragments arranged by size:
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BsrGI T'GTAC_A
Cuts at: 0 6170 9291
Size: 6170 3121
BssHII G'CGCG_C
Cuts at: 0 2888 7163 7309 7311 9291
Size: 2888 4275 146 2 1980
Fragments arranged by size:
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BssSI C'ACGA_G
Cuts at: 0 1624 2848 3544 5746 6151 6996 9291
Size: 1624 1224 696 2202 405 845 2295
Fragments arranged by size:
2295 2202 1624 1224 845 696 405
BstAPI GCAn_nnn'nTGC
Cuts at: 0 972 2718 7608 8550 9147 9291
Size: 972 1746 4890 942 597 144
Fragments arranged by size:
4890 1746 972 942 597 144
BstBI TT'CG_AA
Cuts at: 0 162 4503 9291
Size: 162 4341 4788
Fragments arranged by size:
4788 4341 162
BstEII G'GTnAC_C
Cuts at: 0 8355 8508 9291
Size: 8355 153 783
Fragments arranged by size:
8355 783 153
BstXI CCAn_nnnn'nTGG
Cuts at: 0 126 942 1221 2584 3469 3975 7668 8521
Size: 126 816 279 1363 885 506 3693 853
Cuts at: 8521 8799 9291
Size: 278 492
Fragments arranged by size:
3693 1363 885 853 816 506 492 279
278 126
BstYI r'GATC_y
Cuts at: 0 306 428 2000 2457 2550 2765 3458 4869
Size: 306 122 1572 457 93 215 693 1411
Cuts at: 4869 6117 6133 6282 6785 7019 9291
Size: 1248 16 149 503 234 2272
Fragments arranged by size:
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234 215 149 122 93 16
BstZ17I GTA'TAC
Cuts at: 0 3514 9291
Size: 3514 5777
Bsu36I CC'TnA_GG
Cuts at: 0 740 8320 9291
Size: 740 7580 971
Fragments arranged by size:
7580 971 740
BtgI C'CryG_G
Cuts at: 0 53 1130 1752 3500 5045 5246 5615 8249
Size: 53 1077 622 1748 1545 201 369 2634
Cuts at: 8249 9291
Size: 1042
Fragments arranged by size:
2634 1748 1545 1077 1042 622 369 201
53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 120 4902 5136 6827 8262 8661 9291
Size: 120 4782 234 1691 1435 399 630
Fragments arranged by size:
4782 1691 1435 630 399 234 120
BtsI GCAGTG_nn'
Cuts at: 0 870 2719 7697 8468 9148 9291
Size: 870 1849 4978 771 680 143
Fragments arranged by size:
4978 1849 870 771 680 143
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 8457 8492 9291
Size: 8457 35 799
Fragments arranged by size:
8457 799 35
DraI TTT'AAA
Cuts at: 0 4307 5900 6830 9048 9291
Size: 4307 1593 930 2218 243
Fragments arranged by size:
4307 2218 1593 930 243
DraIII CAC_nnn'GTG
Cuts at: 0 1629 5713 9291
Size: 1629 4084 3578
Fragments arranged by size:
4084 3578 1629
DrdI GACnn_nn'nnGTC
Cuts at: 0 5485 9291
Size: 5485 3806
EaeI y'GGCC_r
Cuts at: 0 59 116 128 1211 1233 2139 5062 5302
Size: 59 57 12 1083 22 906 2923 240
Cuts at: 5302 5465 5618 6261 6954 7592 7742 8127 8312
Size: 163 153 643 693 638 150 385 185
Cuts at: 8312 8465 9291
Size: 153 826
Fragments arranged by size:
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240 185 163 153 153 150 59 57
22 12
EagI C'GGCC_G
Cuts at: 0 5062 5302 9291
Size: 5062 240 3989
Fragments arranged by size:
5062 3989 240
EarI CTCTTCn'nnn_
Cuts at: 0 1802 1922 2326 2356 3352 3385 3845 3974
Size: 1802 120 404 30 996 33 460 129
Cuts at: 3974 4531 5398 5564 5676 5856 8987 9291
Size: 557 867 166 112 180 3131 304
Fragments arranged by size:
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180 166 129 120 112 33 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 32 3145 5677 9291
Size: 32 3113 2532 3614
Fragments arranged by size:
3614 3113 2532 32
EcoICRI GAG'CTC
Cuts at: 0 2319 2349 3531 3897 5285 5724 7460 9291
Size: 2319 30 1182 366 1388 439 1736 1831
Fragments arranged by size:
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Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 694 721 808 832 1021 1123 1297
Size: 445 249 27 87 24 189 102 174
Cuts at: 1297 1519 1672 2080 2214 2365 2467 2665 2728
Size: 222 153 408 134 151 102 198 63
Cuts at: 2728 2821 3211 3232 3492 3731 3760 4381 4676
Size: 93 390 21 260 239 29 621 295
Cuts at: 4676 4810 4822 5077 5145 5202 5242 5626 5788
Size: 134 12 255 68 57 40 384 162
Cuts at: 5788 6352 6694 7264 7368 7476 7510 8251 8524
Size: 564 342 570 104 108 34 741 273
Cuts at: 8524 8684 9118 9291
Size: 160 434 173
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384 342 295 273 260 255 249 239
222 198 189 174 173 162 160 153
151 134 134 108 104 102 102 93
87 68 63 57 40 34 29 27
24 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3091 7438 8218 8689 9091 9094 9291
Size: 3091 4347 780 471 402 3 197
Fragments arranged by size:
4347 3091 780 471 402 197 3
EcoO109I rG'GnC_Cy
Cuts at: 0 123 253 807 819 1434 2121 2196 2637
Size: 123 130 554 12 615 687 75 441
Cuts at: 2637 3716 4205 4485 5276 5293 5511 5958 6935
Size: 1079 489 280 791 17 218 447 977
Cuts at: 6935 6936 7118 8157 8158 8325 9099 9291
Size: 1 182 1039 1 167 774 192
Fragments arranged by size:
1079 1039 977 791 774 687 615 554
489 447 441 280 218 192 182 167
130 123 75 17 12 1 1
EcoRI G'AATT_C
Cuts at: 0 8056 8869 9291
Size: 8056 813 422
Fragments arranged by size:
8056 813 422
EcoRV GAT'ATC
Cuts at: 0 3786 9291
Size: 3786 5505
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 384 6633 7431 9291
Size: 384 6249 798 1860
Fragments arranged by size:
6249 1860 798 384
FseI GG_CCGG'CC
Cuts at: 0 7157 9291
Size: 7157 2134
FspI TGC'GCA
Cuts at: 0 2365 5320 9291
Size: 2365 2955 3971
Fragments arranged by size:
3971 2955 2365
HaeII r_GCGC'y
Cuts at: 0 2136 2481 5429 6989 7265 9291
Size: 2136 345 2948 1560 276 2026
Fragments arranged by size:
2948 2136 2026 1560 345 276
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1254 1286 1324 1356 1405 1437 1501 1533
Size: 1254 32 38 32 49 32 64 32
Cuts at: 1533 2287 2319 2380 2392 2412 2424 2997 3029
Size: 754 32 61 12 20 12 573 32
Cuts at: 3029 3756 3788 4442 4474 4945 4977 5194 5226
Size: 727 32 654 32 471 32 217 32
Cuts at: 5226 5422 5454 5653 5685 7081 7113 7504 7536
Size: 196 32 199 32 1396 32 391 32
Cuts at: 7536 8105 8137 8408 8440 9291
Size: 569 32 271 32 851
Fragments arranged by size:
1396 1254 851 754 727 654 573 569
471 391 271 217 199 196 64 61
49 38 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 20 12 12
HincII GTy'rAC
Cuts at: 0 2175 3030 5796 7886 9291
Size: 2175 855 2766 2090 1405
Fragments arranged by size:
2766 2175 2090 1405 855
HindIII A'AGCT_T
Cuts at: 0 870 1397 8017 9291
Size: 870 527 6620 1274
Fragments arranged by size:
6620 1274 870 527
KasI G'GCGC_C
Cuts at: 0 6985 7261 9291
Size: 6985 276 2030
Fragments arranged by size:
6985 2030 276
KpnI G_GTAC'C
Cuts at: 0 8715 9291
Size: 8715 576
MfeI C'AATT_G
Cuts at: 0 5735 9291
Size: 5735 3556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2743 3375 3779 4086 5055 5477 7059 8913
Size: 2743 632 404 307 969 422 1582 1854
Cuts at: 8913 9291
Size: 378
Fragments arranged by size:
2743 1854 1582 969 632 422 404 378
307
MscI TGG'CCA
Cuts at: 0 1213 1235 2141 5620 6263 7744 8467 9291
Size: 1213 22 906 3479 643 1481 723 824
Fragments arranged by size:
3479 1481 1213 906 824 723 643 22
MslI CAynn'nnrTG
Cuts at: 0 460 685 1124 1207 1781 2017 2033 2035
Size: 460 225 439 83 574 236 16 2
Cuts at: 2035 2947 3467 3635 5176 7669 7690 7841 9227
Size: 912 520 168 1541 2493 21 151 1386
Cuts at: 9227 9291
Size: 64
Fragments arranged by size:
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236 225 168 151 83 64 21 16
2
MspA1I CmG'CkG
Cuts at: 0 14 55 786 1023 1275 1539 1644 1650
Size: 14 41 731 237 252 264 105 6
Cuts at: 1650 1701 1899 1953 2289 2361 2724 3603 4185
Size: 51 198 54 336 72 363 879 582
Cuts at: 4185 5116 5357 5522 6324 6872 6896 6905 6923
Size: 931 241 165 802 548 24 9 18
Cuts at: 6923 6953 6977 7368 7530 7804 9291
Size: 30 24 391 162 274 1487
Fragments arranged by size:
1487 931 879 802 731 582 548 391
363 336 274 264 252 241 237 198
165 162 105 72 54 51 41 30
24 24 18 14 9 6
NaeI GCC'GGC
Cuts at: 0 96 5436 7009 7155 9156 9291
Size: 96 5340 1573 146 2001 135
Fragments arranged by size:
5340 2001 1573 146 135 96
NarI GG'CG_CC
Cuts at: 0 6986 7262 9291
Size: 6986 276 2029
Fragments arranged by size:
6986 2029 276
NcoI C'CATG_G
Cuts at: 0 1130 5615 9291
Size: 1130 4485 3676
Fragments arranged by size:
4485 3676 1130
NgoMIV G'CCGG_C
Cuts at: 0 94 5434 7007 7153 9154 9291
Size: 94 5340 1573 146 2001 137
Fragments arranged by size:
5340 2001 1573 146 137 94
NheI G'CTAG_C
Cuts at: 0 7315 9291
Size: 7315 1976
NotI GC'GGCC_GC
Cuts at: 0 5062 9291
Size: 5062 4229
NruI TCG'CGA
Cuts at: 0 1267 9291
Size: 1267 8024
NspI r_CATG'y
Cuts at: 0 390 690 991 1338 2016 2866 3285 3634
Size: 390 300 301 347 678 850 419 349
Cuts at: 3634 4032 4201 4416 4642 6171 7764 9291
Size: 398 169 215 226 1529 1593 1527
Fragments arranged by size:
1593 1529 1527 850 678 419 398 390
349 347 301 300 226 215 169
PasI CC'CwG_GG
Cuts at: 0 3735 6480 9291
Size: 3735 2745 2811
Fragments arranged by size:
3735 2811 2745
PciI A'CATG_T
Cuts at: 0 1334 2012 4638 6167 7760 9291
Size: 1334 678 2626 1529 1593 1531
Fragments arranged by size:
2626 1593 1531 1529 1334 678
PflMI CCAn_nnn'nTGG
Cuts at: 0 1611 1953 5046 8856 9291
Size: 1611 342 3093 3810 435
Fragments arranged by size:
3810 3093 1611 435 342
PfoI T'CCnGG_A
Cuts at: 0 1610 2324 2633 2954 3932 4769 7015 8630
Size: 1610 714 309 321 978 837 2246 1615
Cuts at: 8630 8975 9134 9291
Size: 345 159 157
Fragments arranged by size:
2246 1615 1610 978 837 714 345 321
309 159 157
PmlI CAC'GTG
Cuts at: 0 3165 5376 7583 8084 8273 9291
Size: 3165 2211 2207 501 189 1018
Fragments arranged by size:
3165 2211 2207 1018 501 189
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 877 909 4508 4540 7715 7747 8804 8836
Size: 877 32 3599 32 3175 32 1057 32
Cuts at: 8836 8890 8922 9291
Size: 54 32 369
Fragments arranged by size:
3599 3175 1057 877 369 54 32 32
32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 123 253 819 1434 2121 2196 2637 3716
Size: 123 130 566 615 687 75 441 1079
Cuts at: 3716 4205 4485 5958 8325 9099 9291
Size: 489 280 1473 2367 774 192
Fragments arranged by size:
2367 1473 1079 774 687 615 566 489
441 280 192 130 123 75
PshAI GACnn'nnGTC
Cuts at: 0 3407 5084 5231 8621 9291
Size: 3407 1677 147 3390 670
Fragments arranged by size:
3407 3390 1677 670 147
PsiI TTA'TAA
Cuts at: 0 4598 9291
Size: 4598 4693
PspOMI G'GGCC_C
Cuts at: 0 3141 5276 5292 6935 7117 8157 9291
Size: 3141 2135 16 1643 182 1040 1134
Fragments arranged by size:
3141 2135 1643 1134 1040 182 16
PspXI vC'TCGA_Gb
Cuts at: 0 2404 7071 9291
Size: 2404 4667 2220
Fragments arranged by size:
4667 2404 2220
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 4445 4477 9291
Size: 4445 32 4814
Fragments arranged by size:
4814 4445 32
PstI C_TGCA'G
Cuts at: 0 437 914 1433 1700 2072 2165 2285 2360
Size: 437 477 519 267 372 93 120 75
Cuts at: 2360 2561 2738 2897 3073 6550 7068 7439 8534
Size: 201 177 159 176 3477 518 371 1095
Cuts at: 8534 8728 9095 9152 9269 9291
Size: 194 367 57 117 22
Fragments arranged by size:
3477 1095 519 518 477 437 372 371
367 267 201 194 177 176 159 120
117 93 75 57 22
PvuII CAG'CTG
Cuts at: 0 786 1023 1275 1650 1701 1899 1953 2289
Size: 786 237 252 375 51 198 54 336
Cuts at: 2289 2361 2724 3603 4185 6324 6977 7804 9291
Size: 72 363 879 582 2139 653 827 1487
Fragments arranged by size:
2139 1487 879 827 786 653 582 375
363 336 252 237 198 72 54 51
SacI G_AGCT'C
Cuts at: 0 2321 2351 3533 3899 5287 5726 7462 9291
Size: 2321 30 1182 366 1388 439 1736 1829
Fragments arranged by size:
2321 1829 1736 1388 1182 439 366 30
SacII CC_GC'GG
Cuts at: 0 56 9291
Size: 56 9235
SanDI GG'GwC_CC
Cuts at: 0 4485 9291
Size: 4485 4806
SapI GCTCTTCn'nnn_
Cuts at: 0 2326 2356 5398 9291
Size: 2326 30 3042 3893
Fragments arranged by size:
3893 3042 2326 30
SbfI CC_TGCA'GG
Cuts at: 0 437 2165 2738 9095 9291
Size: 437 1728 573 6357 196
Fragments arranged by size:
6357 1728 573 437 196
SexAI A'CCwGG_T
Cuts at: 0 8504 9291
Size: 8504 787
SfcI C'TryA_G
Cuts at: 0 433 910 1429 1696 2068 2161 2281 2356
Size: 433 477 519 267 372 93 120 75
Cuts at: 2356 2557 2734 2893 2923 3069 4462 6546 7064
Size: 201 177 159 30 146 1393 2084 518
Cuts at: 7064 7435 8193 8205 8385 8530 8724 9091 9148
Size: 371 758 12 180 145 194 367 57
Cuts at: 9148 9265 9291
Size: 117 26
Fragments arranged by size:
2084 1393 758 519 518 477 433 372
371 367 267 201 194 180 177 159
146 145 120 117 93 75 57 30
26 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 7150 9291
Size: 7150 2141
SfoI GGC'GCC
Cuts at: 0 6987 7263 9291
Size: 6987 276 2028
Fragments arranged by size:
6987 2028 276
SmaI CCC'GGG
Cuts at: 0 7116 7348 7508 9291
Size: 7116 232 160 1783
Fragments arranged by size:
7116 1783 232 160
SmlI C'TyrA_G
Cuts at: 0 2404 3001 3019 3079 4711 6343 7071 7876
Size: 2404 597 18 60 1632 1632 728 805
Cuts at: 7876 9291
Size: 1415
Fragments arranged by size:
2404 1632 1632 1415 805 728 597 60
18
SnaBI TAC'GTA
Cuts at: 0 3950 8949 9291
Size: 3950 4999 342
Fragments arranged by size:
4999 3950 342
SpeI A'CTAG_T
Cuts at: 0 5928 9291
Size: 5928 3363
SphI G_CATG'C
Cuts at: 0 2866 3634 4416 9291
Size: 2866 768 782 4875
Fragments arranged by size:
4875 2866 782 768
StuI AGG'CCT
Cuts at: 0 563 1082 2581 5126 5668 9291
Size: 563 519 1499 2545 542 3623
Fragments arranged by size:
3623 2545 1499 563 542 519
StyI C'CwwG_G
Cuts at: 0 119 926 1130 1592 2541 4881 5615 5961
Size: 119 807 204 462 949 2340 734 346
Cuts at: 5961 7301 7719 8328 9068 9291
Size: 1340 418 609 740 223
Fragments arranged by size:
2340 1340 949 807 740 734 609 462
418 346 223 204 119
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 697 2047 3538 4615 4662 5628 7633 8527
Size: 697 1350 1491 1077 47 966 2005 894
Cuts at: 8527 9291
Size: 764
Fragments arranged by size:
2005 1491 1350 1077 966 894 764 697
47
TatI w'GTAC_w
Cuts at: 0 3098 3710 6170 9291
Size: 3098 612 2460 3121
Fragments arranged by size:
3121 3098 2460 612
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1270 1302 1375 1407 2239 2271 2584 2616
Size: 1270 32 73 32 832 32 313 32
Cuts at: 2616 6388 6420 9064 9096 9291
Size: 3772 32 2644 32 195
Fragments arranged by size:
3772 2644 1270 832 313 195 73 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 8506 9291
Size: 8506 785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 488 819 1221 3447 5169 5871 7552 8460
Size: 488 331 402 2226 1722 702 1681 908
Cuts at: 8460 8595 9291
Size: 135 696
Fragments arranged by size:
2226 1722 1681 908 702 696 488 402
331 135
XhoI C'TCGA_G
Cuts at: 0 2404 3079 7071 9291
Size: 2404 675 3992 2220
Fragments arranged by size:
3992 2404 2220 675
XmaI C'CCGG_G
Cuts at: 0 7114 7346 7506 9291
Size: 7114 232 160 1785
Fragments arranged by size:
7114 1785 232 160
XmnI GAAnn'nnTTC
Cuts at: 0 872 1763 2443 3880 3983 6337 6616 9291
Size: 872 891 680 1437 103 2354 279 2675
Fragments arranged by size:
2675 2354 1437 891 872 680 279 103
ZraI GAC'GTC
Cuts at: 0 2532 3246 4931 9291
Size: 2532 714 1685 4360
Fragments arranged by size:
4360 2532 1685 714
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NotI
NruI NspI PasI PciI PflMI PfoI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuII
SacI SacII SanDI SapI SbfI SexAI SfcI SfiI
SfoI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AgeI AscI AseI AsiSI BaeI BfrBI BplI BsiWI
BspEI ClaI FspAI HpaI MluI NdeI NsiI PacI
PmeI PvuI RsrII SalI ScaI SgfI SgrAI SrfI
SspI SwaI XbaI