(Circular) (Six-base) MAPSORT of: pFN21AE3267.seq Check: 4117 from: 1 to: 6691 pFN21AE3267 6691 bp With 182 enzymes: SgfI * July 6, 2012 17:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4501 278 Size: 53 83 186 936 2965 2468 Fragments arranged by size: 2965 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4034 1937 Size: 2097 4594 Acc65I G'GTAC_C Cuts at: 4012 4012 Size: 6691 AclI AA'CG_TT Cuts at: 1563 4818 5191 1563 Size: 3255 373 3063 Fragments arranged by size: 3255 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3129 4754 6138 6406 1632 Size: 1497 1625 1384 268 1917 Fragments arranged by size: 1917 1625 1497 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6494 AflIII A'CryG_T Cuts at: 1203 5590 6567 1203 Size: 4387 977 1327 Fragments arranged by size: 4387 1327 977 AgeI A'CCGG_T Cuts at: 5522 5522 Size: 6691 AhdI GACnn_n'nnGTC Cuts at: 5420 5420 Size: 6691 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6691 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6691 AlwNI CAG_nnn'CTG Cuts at: 4434 6006 4434 Size: 1572 5119 ApaLI G'TGCA_C Cuts at: 4748 5904 4748 Size: 1156 5535 ApoI r'AATT_y Cuts at: 1493 2412 3209 4000 4226 4378 6487 1493 Size: 919 797 791 226 152 2109 1697 Fragments arranged by size: 2109 1697 919 797 791 226 152 AseI AT'TA_AT Cuts at: 160 1032 2012 5245 160 Size: 872 980 3233 1606 Fragments arranged by size: 3233 1606 980 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6691 AvaI C'yCGr_G Cuts at: 1870 1943 4016 1870 Size: 73 2073 4545 Fragments arranged by size: 4545 2073 73 BamHI G'GATC_C Cuts at: 2575 3103 4021 4388 2575 Size: 528 918 367 4878 Fragments arranged by size: 4878 918 528 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3039 4012 5467 618 Size: 325 217 627 1252 973 1455 1842 Fragments arranged by size: 1842 1455 1252 973 627 325 217 BanII G_rGCy'C Cuts at: 1391 2068 2514 4010 1391 Size: 677 446 1496 4072 Fragments arranged by size: 4072 1496 677 446 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5720 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2950 2984 4882 4916 1903 Size: 34 1013 34 1898 34 3678 Fragments arranged by size: 3678 1898 1013 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4583 5793 4583 Size: 1210 5481 BclI T'GATC_A Cuts at: 1548 2440 2923 1548 Size: 892 483 5316 Fragments arranged by size: 5316 892 483 BfrBI ATG'CAT Cuts at: 2059 2059 Size: 6691 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5302 136 Size: 107 122 71 631 605 3630 1525 Fragments arranged by size: 3630 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6686 6686 Size: 6691 BlpI GC'TnA_GC Cuts at: 4108 4108 Size: 6691 Bme1580I G_kGCm'C Cuts at: 1790 2132 3042 3478 4752 5908 1790 Size: 342 910 436 1274 1156 2573 Fragments arranged by size: 2573 1274 1156 910 436 342 BmgBI CAC'GTC Cuts at: 1318 3926 1318 Size: 2608 4083 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3839 4422 5380 Size: 416 53 83 399 24 2422 583 958 Cuts at: 5380 442 Size: 1753 Fragments arranged by size: 2422 1753 958 583 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6691 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2486 3608 5351 1239 Size: 1247 1122 1743 2579 Fragments arranged by size: 2579 1743 1247 1122 Bpu10I CC'TnA_GC Cuts at: 2154 3005 2154 Size: 851 5840 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2041 2235 4816 5681 5979 2041 Size: 194 2581 865 298 2753 Fragments arranged by size: 2753 2581 865 298 194 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3584 5354 882 Size: 617 2085 1770 2219 Fragments arranged by size: 2219 2085 1770 617 BsaAI yAC'GTr Cuts at: 493 1204 4482 493 Size: 711 3278 2702 Fragments arranged by size: 3278 2702 711 BsaBI GATnn'nnATC Cuts at: 2439 6685 2439 Size: 4246 2445 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4498 4880 5515 275 Size: 53 83 186 936 2965 382 635 1451 Fragments arranged by size: 2965 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5124 5522 5796 5943 6354 5124 Size: 398 274 147 411 5461 Fragments arranged by size: 5461 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2111 2141 1178 Size: 30 165 30 708 30 5728 Fragments arranged by size: 5728 708 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2309 3014 1186 Size: 1123 705 4863 Fragments arranged by size: 4863 1123 705 BseYI C'CCAG_C Cuts at: 5894 6390 5894 Size: 496 6195 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2672 1163 Size: 573 936 5182 Fragments arranged by size: 5182 936 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4141 4902 5930 1540 Size: 275 186 2140 761 1028 2301 Fragments arranged by size: 2301 2140 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2068 4752 4837 5908 2068 Size: 2684 85 1071 2851 Fragments arranged by size: 2851 2684 1071 85 BsmI GAATG_Cn' Cuts at: 2618 3386 3518 4202 4295 2618 Size: 768 132 684 93 5014 Fragments arranged by size: 5014 768 684 132 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2630 2630 Size: 6691 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2068 2132 2514 3042 3478 4010 4752 Size: 399 278 64 382 528 436 532 742 Cuts at: 4752 4837 5908 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 532 528 436 399 382 278 85 64 BspEI T'CCGG_A Cuts at: 6354 6354 Size: 6691 BspHI T'CATG_A Cuts at: 3898 4580 6310 3898 Size: 682 1730 4279 Fragments arranged by size: 4279 1730 682 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4046 844 Size: 3202 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4578 1394 Size: 429 2755 3507 Fragments arranged by size: 3507 2755 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5186 5360 65 Size: 1183 3938 174 1396 Fragments arranged by size: 3938 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5335 5522 1700 Size: 110 3525 187 2869 Fragments arranged by size: 3525 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6691 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6691 BssSI C'ACGA_G Cuts at: 4751 5763 4751 Size: 1012 5679 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6691 BstBI TT'CG_AA Cuts at: 2204 5495 2204 Size: 3291 3400 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6691 BstYI r'GATC_y Cuts at: 1972 2575 3103 3888 4021 4388 4773 4790 6242 Size: 603 528 785 133 367 385 17 1452 Cuts at: 6242 6328 6340 6686 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 785 603 528 385 367 346 133 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5200 Fragments arranged by size: 5200 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5952 Fragments arranged by size: 5952 683 56 BtsI GCAGTG_nn' Cuts at: 2684 2904 3183 4207 5001 5021 2684 Size: 220 279 1024 794 20 4354 Fragments arranged by size: 4354 1024 794 279 220 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6691 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6656 DraI TTT'AAA Cuts at: 2079 2835 3995 2079 Size: 756 1160 4775 Fragments arranged by size: 4775 1160 756 DraIII CAC_nnn'GTG Cuts at: 2234 2234 Size: 6691 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5698 809 Size: 507 4382 1802 Fragments arranged by size: 4382 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4138 5027 6656 Size: 54 1425 50 275 18 2308 889 1629 Cuts at: 6656 8 Size: 43 Fragments arranged by size: 2308 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4138 1537 Size: 275 2326 4090 Fragments arranged by size: 4090 2326 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4621 1353 Size: 3268 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5278 5652 5798 387 Size: 1064 138 3689 374 146 1280 Fragments arranged by size: 3689 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2066 2066 Size: 6691 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2486 3129 3608 4754 5351 6138 6406 Size: 393 854 643 479 1146 597 787 268 Cuts at: 6406 1239 Size: 1524 Fragments arranged by size: 1524 1146 854 787 643 597 479 393 268 EcoRI G'AATT_C Cuts at: 4000 4378 4000 Size: 378 6313 EcoRV GAT'ATC Cuts at: 3763 3763 Size: 6691 FspI TGC'GCA Cuts at: 5197 5197 Size: 6691 HaeII r_GCGC'y Cuts at: 1678 5838 1678 Size: 4160 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3880 3912 5332 5364 5406 Size: 32 135 32 2308 32 1420 32 42 Cuts at: 5406 5438 1373 Size: 32 2626 Fragments arranged by size: 2626 2308 1420 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3394 3681 4035 4281 669 Size: 988 281 1456 287 354 246 3079 Fragments arranged by size: 3079 1456 988 354 287 281 246 HindIII A'AGCT_T Cuts at: 748 2484 748 Size: 1736 4955 HpaI GTT'AAC Cuts at: 4281 4281 Size: 6691 KpnI G_GTAC'C Cuts at: 4016 4016 Size: 6691 MfeI C'AATT_G Cuts at: 4290 4290 Size: 6691 MluI A'CGCG_T Cuts at: 6567 6567 Size: 6691 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2895 3081 5805 5989 1305 Size: 1590 186 2724 184 2007 Fragments arranged by size: 2724 2007 1590 186 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4869 Fragments arranged by size: 4869 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4649 5008 5167 518 Size: 648 3483 359 159 2042 Fragments arranged by size: 3483 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2521 4107 4784 5932 6177 1606 Size: 915 1586 677 1148 245 2120 Fragments arranged by size: 2120 1586 1148 915 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5200 Fragments arranged by size: 5200 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6691 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6691 NotI GC'GGCC_GC Cuts at: 4138 4138 Size: 6691 NsiI A_TGCA'T Cuts at: 2061 2061 Size: 6691 NspI r_CATG'y Cuts at: 1132 1229 2573 2802 2981 4049 5594 1132 Size: 97 1344 229 179 1068 1545 2229 Fragments arranged by size: 2229 1545 1344 1068 229 179 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6691 PciI A'CATG_T Cuts at: 5590 5590 Size: 6691 PflMI CCAn_nnn'nTGG Cuts at: 1108 2234 1108 Size: 1126 5565 PfoI T'CCnGG_A Cuts at: 3507 3507 Size: 6691 PmeI GTTT'AAAC Cuts at: 3995 3995 Size: 6691 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4760 4792 6273 6305 4760 Size: 32 1481 32 5146 Fragments arranged by size: 5146 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6691 PsiI TTA'TAA Cuts at: 2360 2723 2951 4261 2360 Size: 363 228 1310 4790 Fragments arranged by size: 4790 1310 363 228 PstI C_TGCA'G Cuts at: 830 3122 3140 4043 830 Size: 2292 18 903 3478 Fragments arranged by size: 3478 2292 903 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6691 PvuII CAG'CTG Cuts at: 2521 2521 Size: 6691 SacI G_AGCT'C Cuts at: 2068 2068 Size: 6691 SalI G'TCGA_C Cuts at: 1936 4033 1936 Size: 2097 4594 SbfI CC_TGCA'GG Cuts at: 4043 4043 Size: 6691 ScaI AGT'ACT Cuts at: 3134 4939 6651 3134 Size: 1805 1712 3174 Fragments arranged by size: 3174 1805 1712 SexAI A'CCwGG_T Cuts at: 2601 2601 Size: 6691 SfcI C'TryA_G Cuts at: 826 1045 3118 3136 3860 4039 5174 5855 6046 Size: 219 2073 18 724 179 1135 681 191 Cuts at: 6046 826 Size: 1471 Fragments arranged by size: 2073 1471 1135 724 681 219 191 179 18 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6691 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6691 SmaI CCC'GGG Cuts at: 1872 4018 1872 Size: 2146 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2020 2250 4795 5696 5958 820 Size: 197 926 77 230 2545 901 262 1553 Fragments arranged by size: 2545 1553 926 901 262 230 197 77 SnaBI TAC'GTA Cuts at: 493 4482 493 Size: 3989 2702 SpeI A'CTAG_T Cuts at: 152 2531 152 Size: 2379 4312 SphI G_CATG'C Cuts at: 1132 4049 1132 Size: 2917 3774 SspI AAT'ATT Cuts at: 5 52 3411 3941 4615 5 Size: 47 3359 530 674 2081 Fragments arranged by size: 3359 2081 674 530 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6691 StyI C'CwwG_G Cuts at: 513 1065 2004 2132 4130 513 Size: 552 939 128 1998 3074 Fragments arranged by size: 3074 1998 939 552 128 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3352 4718 4735 4888 5073 5518 325 Size: 1255 1772 1366 17 153 185 445 1498 Fragments arranged by size: 1772 1498 1366 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2801 3132 4937 Size: 274 80 33 51 1443 824 331 1805 Cuts at: 4937 6649 96 Size: 1712 138 Fragments arranged by size: 1805 1712 1443 824 331 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2111 2143 3088 3120 6651 6683 2111 Size: 32 945 32 3531 32 2119 Fragments arranged by size: 3531 2119 945 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6691 XbaI T'CTAG_A Cuts at: 4027 4027 Size: 6691 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6691 XmaI C'CCGG_G Cuts at: 1870 4016 1870 Size: 2146 4545 XmnI GAAnn'nnTTC Cuts at: 3150 4820 3150 Size: 1670 5021 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4499 276 Size: 53 83 186 936 2965 2468 Fragments arranged by size: 2965 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstXI BstZ17I Bsu36I EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI