################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII AccI AccII AccIII AclI AflII AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI AspEI AviII BamHI BbrPI BcgI BciVI BfrI BglI BglII BsaAI BsaI BseAI BsiEI BsiWI BspDI BspEI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KspI MfeI MflI MluI MroI MunI MvnI NcoI NdeI NheI NotI NruI NsiI NspV PacI PflMI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Van91I XbaI XcmI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 258 GGTACC G^GTACC AflIII 1367 ACATGT A^C[GA][CT]GT AgeI 595 ACCGGT A^CCGGT Aor51HI 1022 AGCGCT AGC^GCT Asp700I 402 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 258 GGTACC G^GTACC AspI 1029 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1296 CCTAGG C^CTAGG BbeI 736 GGCGCC GGCGC^C BclI 1247 TGATCA T^GATCA BlnI 1296 CCTAGG C^CTAGG BsaBI 945 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 1128 CTGCAC GTGCAG(16/14) BsmI 539 GAATGC GAATGC(1/-1) Bsp1407I 90 TGTACA T^GTACA BspLU11I 1367 ACATGT A^CATGT BsrBI 1405 CCGCTC CCGCTC(-3/-3) BsrGI 90 TGTACA T^GTACA BstAPI 195 GCAAGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 499 CATCC GGATG(2/0) DraIII 211 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 145 GACCTGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1022 AGCGCT AGC^GCT EcoNI 243 CCTTGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 1247 TGATCA T^GATCA FokI 499 CATCC GGATG(9/13) HincII 301 GTTGAC GT[CT]^[GA]AC HindII 301 GTTGAC GT[CT]^[GA]AC KasI 736 GGCGCC G^GCGCC KpnI 258 GGTACC GGTAC^C MamI 945 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 741 CATGCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 1183 GCCGGC GCC^GGC NarI 736 GGCGCC GG^CGCC NgoMIV 1183 GCCGGC G^CCGGC PaeR7I 1410 CTCGAG C^TCGAG PflFI 1029 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 595 ACCGGT A^CCGGT PleI 1006 GAGTC GAGTC(4/5) PshAI 324 GACGGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 415 CTGCAG CTGCA^G PvuII 184 CAGCTG CAG^CTG SacI 726 GAGCTC GAGCT^C ScaI 835 AGTACT AGT^ACT SfoI 736 GGCGCC GGC^GCC SmlI 1410 CTCGAG C^T[CT][GA]AG SspBI 90 TGTACA T^GTACA SspI 368 AATATT AAT^ATT Tth111I 1029 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1410 CTCGAG C^TCGAG XmnI 402 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 553 GAATTT [GA]^AATT[CT] 576 AAATTT AlwNI 184 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 1051 CAGCTCCTG ApoI 553 GAATTT [GA]^AATT[CT] 576 AAATTT AspHI 726 GAGCTC G[AT]GC[AT]^C 778 GTGCTC AvaI 1003 CCCGAG C^[CT]CG[GA]G 1410 CTCGAG BfaI 134 CTAG C^TAG 1297 CTAG BlpI 220 GCTGAGC GC^T[ACGT]AGC 780 GCTCAGC BpmI 190 CTGGAG CTGGAG(16/14) 1569 CTGGAG Bpu1102I 220 GCTGAGC GC^T[ACGT]AGC 780 GCTCAGC BsaWI 595 ACCGGT [AT]^CCGG[AT] 881 ACCGGA BsiHKAI 726 GAGCTC G[AT]GC[AT]^C 778 GTGCTC BsmBI 322 GAGACG CGTCTC(1/5) 824 GAGACG BsoBI 1003 CCCGAG C^[CT]CG[GA]G 1410 CTCGAG BspHI 644 TCATGA T^CATGA 1250 TCATGA BspMI 744 GCAGGT ACCTGC(4/8) 753 GCAGGT BsrDI 374 CATTGC GCAATG(2/0) 974 GCAATG CelII 220 GCTGAGC GC^T[ACGT]AGC 780 GCTCAGC EarI 1077 CTCTTC CTCTTC(1/4) 32 GAAGAG FauI 1404 CCCGC CCCGC(4/6) 662 GCGGG Ksp632I 1077 CTCTTC CTCTTC(1/4) 32 GAAGAG MaeI 134 CTAG C^TAG 1297 CTAG NspI 970 ACATGC [GA]CATG^[CT] 1367 ACATGT RcaI 644 TCATGA T^CATGA 1250 TCATGA SexAI 146 ACCTGGT A^CC[AT]GGT 1270 ACCAGGT Tsp45I 795 GTCAC ^GT[GC]AC 1103 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BmyI BpuAI BsaHI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I