(Circular) (Six-base) MAPSORT of: pFN21AE6749.seq Check: 2327 from: 1 to: 6331 pFN21AE6749 6331 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2740 2740 Size: 6331 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4141 278 Size: 53 83 186 936 2605 2468 Fragments arranged by size: 2605 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3674 1937 Size: 1737 4594 Acc65I G'GTAC_C Cuts at: 2263 3652 2263 Size: 1389 4942 AclI AA'CG_TT Cuts at: 1563 4458 4831 1563 Size: 2895 373 3063 Fragments arranged by size: 3063 2895 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2040 2316 3051 4394 5778 6046 1632 Size: 408 276 735 1343 1384 268 1917 Fragments arranged by size: 1917 1384 1343 735 408 276 268 AfeI AGC'GCT Cuts at: 3029 3029 Size: 6331 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6134 AflIII A'CryG_T Cuts at: 1203 3372 5230 6207 1203 Size: 2169 1858 977 1327 Fragments arranged by size: 2169 1858 1327 977 AgeI A'CCGG_T Cuts at: 2600 5162 2600 Size: 2562 3769 AhdI GACnn_n'nnGTC Cuts at: 5060 5060 Size: 6331 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6331 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6331 AlwNI CAG_nnn'CTG Cuts at: 2194 3061 4074 5646 2194 Size: 867 1013 1572 2879 Fragments arranged by size: 2879 1572 1013 867 ApaLI G'TGCA_C Cuts at: 4388 5544 4388 Size: 1156 5175 ApoI r'AATT_y Cuts at: 1493 2558 2581 3640 3866 4018 6127 1493 Size: 1065 23 1059 226 152 2109 1697 Fragments arranged by size: 2109 1697 1065 1059 226 152 23 AseI AT'TA_AT Cuts at: 160 1032 4885 160 Size: 872 3853 1606 Fragments arranged by size: 3853 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6331 AvaI C'yCGr_G Cuts at: 1870 1943 3008 3415 3656 1870 Size: 73 1065 407 241 4545 Fragments arranged by size: 4545 1065 407 241 73 AvrII C'CTAG_G Cuts at: 3301 3301 Size: 6331 BamHI G'GATC_C Cuts at: 3661 4028 3661 Size: 367 5964 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2263 2624 2741 3652 5107 Size: 325 217 627 476 361 117 911 1455 Cuts at: 5107 618 Size: 1842 Fragments arranged by size: 1842 1455 911 627 476 361 325 217 117 BanII G_rGCy'C Cuts at: 1391 2735 3009 3422 3473 3650 1391 Size: 1344 274 413 51 177 4072 Fragments arranged by size: 4072 1344 413 274 177 51 BbeI G_GCGC'C Cuts at: 2745 2745 Size: 6331 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2152 2755 3057 3084 3369 928 Size: 971 253 603 302 27 285 3890 Fragments arranged by size: 3890 971 603 302 285 253 27 BbvCI CC'TCA_GC Cuts at: 3228 3394 3623 3228 Size: 166 229 5936 Fragments arranged by size: 5936 229 166 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4522 4556 1903 Size: 34 2585 34 3678 Fragments arranged by size: 3678 2585 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4223 5433 4223 Size: 1210 5121 BclI T'GATC_A Cuts at: 1548 3252 1548 Size: 1704 4627 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4942 136 Size: 107 122 71 631 605 3270 1525 Fragments arranged by size: 3270 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6326 6326 Size: 6331 BlpI GC'TnA_GC Cuts at: 2226 2786 3748 2226 Size: 560 962 4809 Fragments arranged by size: 4809 962 560 Bme1580I G_kGCm'C Cuts at: 1790 4392 5548 1790 Size: 2602 1156 2573 Fragments arranged by size: 2602 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6331 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3138 3210 4062 Size: 416 53 83 399 24 1721 72 852 Cuts at: 4062 5020 442 Size: 958 1753 Fragments arranged by size: 1753 1721 958 852 416 399 83 72 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6331 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2216 3595 4991 1239 Size: 977 1379 1396 2579 Fragments arranged by size: 2579 1396 1379 977 Bpu10I CC'TnA_GC Cuts at: 3228 3394 3623 3228 Size: 166 229 5936 Fragments arranged by size: 5936 229 166 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4456 5321 5619 4456 Size: 865 298 5168 Fragments arranged by size: 5168 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4994 882 Size: 617 3495 2219 Fragments arranged by size: 3495 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4122 493 Size: 711 2918 2702 Fragments arranged by size: 2918 2702 711 BsaBI GATnn'nnATC Cuts at: 2954 6325 2954 Size: 3371 2960 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2334 2742 2832 4138 Size: 53 83 186 936 801 408 90 1306 Cuts at: 4138 4520 5155 275 Size: 382 635 1451 Fragments arranged by size: 1451 1306 936 801 635 408 382 186 90 83 53 BsaWI w'CCGG_w Cuts at: 2600 2886 4764 5162 5436 5583 5994 2600 Size: 286 1878 398 274 147 411 2937 Fragments arranged by size: 2937 1878 411 398 286 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3274 3304 1178 Size: 30 165 30 1871 30 4205 Fragments arranged by size: 4205 1871 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2657 3064 3168 3592 1186 Size: 1471 407 104 424 3925 Fragments arranged by size: 3925 1471 424 407 104 BseYI C'CCAG_C Cuts at: 3054 5534 6030 3054 Size: 2480 496 3355 Fragments arranged by size: 3355 2480 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3118 1163 Size: 573 1382 4376 Fragments arranged by size: 4376 1382 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3781 4542 5570 1540 Size: 275 186 1780 761 1028 2301 Fragments arranged by size: 2301 1780 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2735 2787 4392 4477 5548 2735 Size: 52 1605 85 1071 3518 Fragments arranged by size: 3518 1605 1071 85 52 BsmI GAATG_Cn' Cuts at: 2550 3842 3935 2550 Size: 1292 93 4946 Fragments arranged by size: 4946 1292 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2321 2823 2321 Size: 502 5829 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2735 2787 3009 3422 3473 3650 4392 Size: 399 945 52 222 413 51 177 742 Cuts at: 4392 4477 5548 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 945 742 413 399 222 177 85 52 51 BspEI T'CCGG_A Cuts at: 5994 5994 Size: 6331 BspHI T'CATG_A Cuts at: 2649 3255 4220 5950 2649 Size: 606 965 1730 3030 Fragments arranged by size: 3030 1730 965 606 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2740 2749 3686 844 Size: 1896 9 937 3489 Fragments arranged by size: 3489 1896 937 9 BsrBI CCG'CTC Cuts at: 1394 1823 3412 4218 1394 Size: 429 1589 806 3507 Fragments arranged by size: 3507 1589 806 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2378 2986 4826 5000 65 Size: 1183 1130 608 1840 174 1396 Fragments arranged by size: 1840 1396 1183 1130 608 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2600 3188 3569 4975 5162 1700 Size: 110 790 588 381 1406 187 2869 Fragments arranged by size: 2869 1406 790 588 381 187 110 BsrGI T'GTAC_A Cuts at: 96 2095 96 Size: 1999 4332 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6331 BssSI C'ACGA_G Cuts at: 4391 5403 4391 Size: 1012 5319 BstAPI GCAn_nnn'nTGC Cuts at: 794 2206 794 Size: 1412 4919 BstBI TT'CG_AA Cuts at: 5135 5135 Size: 6331 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6331 BstYI r'GATC_y Cuts at: 1972 3661 4028 4413 4430 5882 5968 5980 6326 Size: 1689 367 385 17 1452 86 12 346 Cuts at: 6326 1972 Size: 1977 Fragments arranged by size: 1977 1689 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5779 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5592 Fragments arranged by size: 5592 683 56 BtsI GCAGTG_nn' Cuts at: 3847 4641 4661 3847 Size: 794 20 5517 Fragments arranged by size: 5517 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6331 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6296 DraI TTT'AAA Cuts at: 3635 3635 Size: 6331 DraIII CAC_nnn'GTG Cuts at: 2221 2221 Size: 6331 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2156 5338 809 Size: 507 840 3182 1802 Fragments arranged by size: 3182 1802 840 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2081 2481 2725 Size: 54 1425 50 275 18 251 400 244 Cuts at: 2725 2808 3022 3186 3384 3478 3778 4667 6296 Size: 83 214 164 198 94 300 889 1629 Cuts at: 6296 8 Size: 43 Fragments arranged by size: 1629 1425 889 400 300 275 251 244 214 198 164 94 83 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3778 1537 Size: 275 1966 4090 Fragments arranged by size: 4090 1966 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2032 3088 4261 1353 Size: 679 1056 1173 3423 Fragments arranged by size: 3423 1173 1056 679 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4918 5292 5438 387 Size: 1064 138 3329 374 146 1280 Fragments arranged by size: 3329 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2733 2733 Size: 6331 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2040 2216 2316 3051 3595 4394 4991 Size: 393 408 176 100 735 544 799 597 Cuts at: 4991 5778 6046 1239 Size: 787 268 1524 Fragments arranged by size: 1524 799 787 735 597 544 408 393 268 176 100 EcoNI CCTnn'n_nnAGG Cuts at: 2252 2252 Size: 6331 EcoO109I rG'GnC_Cy Cuts at: 2428 2698 2814 2428 Size: 270 116 5945 Fragments arranged by size: 5945 270 116 EcoRI G'AATT_C Cuts at: 3640 4018 3640 Size: 378 5953 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2779 2779 Size: 6331 FspI TGC'GCA Cuts at: 4837 4837 Size: 6331 HaeII r_GCGC'y Cuts at: 1678 2745 3031 3173 5478 1678 Size: 1067 286 142 2305 2531 Fragments arranged by size: 2531 2305 1067 286 142 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2996 3028 3409 3441 4972 Size: 32 135 32 1424 32 381 32 1531 Cuts at: 4972 5004 5046 5078 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1531 1424 381 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2308 3675 3921 669 Size: 988 281 370 1367 246 3079 Fragments arranged by size: 3079 1367 988 370 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6331 HpaI GTT'AAC Cuts at: 3921 3921 Size: 6331 KasI G'GCGC_C Cuts at: 2741 2741 Size: 6331 KpnI G_GTAC'C Cuts at: 2267 3656 2267 Size: 1389 4942 MfeI C'AATT_G Cuts at: 3930 3930 Size: 6331 MluI A'CGCG_T Cuts at: 6207 6207 Size: 6331 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2159 5445 5629 1305 Size: 854 3286 184 2007 Fragments arranged by size: 3286 2007 854 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2483 2810 3024 10 Size: 54 1425 343 651 327 214 3317 Fragments arranged by size: 3317 1425 651 343 327 214 54 MslI CAynn'nnrTG Cuts at: 518 1166 2750 4289 4648 4807 518 Size: 648 1584 1539 359 159 2042 Fragments arranged by size: 2042 1584 1539 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2191 2345 2667 2806 3747 4424 5572 5817 Size: 585 154 322 139 941 677 1148 245 Cuts at: 5817 1606 Size: 2120 Fragments arranged by size: 2120 1148 941 677 585 322 245 154 139 NaeI GCC'GGC Cuts at: 3190 3190 Size: 6331 NarI GG'CG_CC Cuts at: 2742 2742 Size: 6331 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5779 NdeI CA'TA_TG Cuts at: 387 387 Size: 6331 NgoMIV G'CCGG_C Cuts at: 3188 3188 Size: 6331 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6331 NotI GC'GGCC_GC Cuts at: 3778 3778 Size: 6331 NspI r_CATG'y Cuts at: 1132 1229 2979 3376 3689 5234 1132 Size: 97 1750 397 313 1545 2229 Fragments arranged by size: 2229 1750 1545 397 313 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6331 PciI A'CATG_T Cuts at: 3372 5230 3372 Size: 1858 4473 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6331 PmeI GTTT'AAAC Cuts at: 3635 3635 Size: 6331 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4400 4432 5913 5945 4400 Size: 32 1481 32 4786 Fragments arranged by size: 4786 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2428 2698 2814 2428 Size: 270 116 5945 Fragments arranged by size: 5945 270 116 PshAI GACnn'nnGTC Cuts at: 1613 2333 1613 Size: 720 5611 PsiI TTA'TAA Cuts at: 3901 3901 Size: 6331 PstI C_TGCA'G Cuts at: 830 2424 3683 830 Size: 1594 1259 3478 Fragments arranged by size: 3478 1594 1259 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6331 PvuII CAG'CTG Cuts at: 2191 2191 Size: 6331 SacI G_AGCT'C Cuts at: 2735 2735 Size: 6331 SalI G'TCGA_C Cuts at: 1936 3673 1936 Size: 1737 4594 SbfI CC_TGCA'GG Cuts at: 3683 3683 Size: 6331 ScaI AGT'ACT Cuts at: 2842 4579 6291 2842 Size: 1737 1712 2882 Fragments arranged by size: 2882 1737 1712 SexAI A'CCwGG_T Cuts at: 2151 3275 2151 Size: 1124 5207 SfcI C'TryA_G Cuts at: 826 1045 2420 2495 3499 3679 4814 5495 5686 Size: 219 1375 75 1004 180 1135 681 191 Cuts at: 5686 826 Size: 1471 Fragments arranged by size: 1471 1375 1135 1004 681 219 191 180 75 SfoI GGC'GCC Cuts at: 2743 2743 Size: 6331 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6331 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6331 SmaI CCC'GGG Cuts at: 1872 3658 1872 Size: 1786 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3415 4435 5336 5598 820 Size: 197 926 1472 1020 901 262 1553 Fragments arranged by size: 1553 1472 1020 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4122 493 Size: 3629 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6331 SphI G_CATG'C Cuts at: 1132 3689 1132 Size: 2557 3774 SspI AAT'ATT Cuts at: 5 52 2375 4255 5 Size: 47 2323 1880 2081 Fragments arranged by size: 2323 2081 1880 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6331 StyI C'CwwG_G Cuts at: 513 1065 2539 3301 3487 3770 513 Size: 552 1474 762 186 283 3074 Fragments arranged by size: 3074 1474 762 552 283 186 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3500 4358 4375 4528 4713 5158 325 Size: 1255 1920 858 17 153 185 445 1498 Fragments arranged by size: 1920 1498 1255 858 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2095 2117 2840 Size: 274 80 33 51 1443 118 22 723 Cuts at: 2840 4577 6289 96 Size: 1737 1712 138 Fragments arranged by size: 1737 1712 1443 723 274 138 118 80 51 33 22 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3362 3394 6291 6323 3362 Size: 32 2897 32 3370 Fragments arranged by size: 3370 2897 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3037 1316 Size: 1721 4610 XbaI T'CTAG_A Cuts at: 3667 3667 Size: 6331 XhoI C'TCGA_G Cuts at: 1943 3415 1943 Size: 1472 4859 XmaI C'CCGG_G Cuts at: 1870 3656 1870 Size: 1786 4545 XmnI GAAnn'nnTTC Cuts at: 2411 4460 2411 Size: 2049 4282 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4139 276 Size: 53 83 186 936 2605 2468 Fragments arranged by size: 2605 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI XcmI