(Linear) (Six-base) MAPSORT of: KIEE6749.seq Check: 4795 from: 1 to: 1626 KIEE6749.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 735 1626 Size: 735 891 Acc65I G'GTAC_C Cuts at: 0 258 1626 Size: 258 1368 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 35 311 1046 1626 Size: 35 276 735 580 Fragments arranged by size: 735 580 276 35 AfeI AGC'GCT Cuts at: 0 1024 1626 Size: 1024 602 AflIII A'CryG_T Cuts at: 0 1367 1626 Size: 1367 259 AgeI A'CCGG_T Cuts at: 0 595 1626 Size: 595 1031 AlwNI CAG_nnn'CTG Cuts at: 0 189 1056 1626 Size: 189 867 570 Fragments arranged by size: 867 570 189 ApoI r'AATT_y Cuts at: 0 553 576 1626 Size: 553 23 1050 Fragments arranged by size: 1050 553 23 AvaI C'yCGr_G Cuts at: 0 1003 1410 1626 Size: 1003 407 216 Fragments arranged by size: 1003 407 216 AvrII C'CTAG_G Cuts at: 0 1296 1626 Size: 1296 330 BanI G'GyrC_C Cuts at: 0 258 619 736 1626 Size: 258 361 117 890 Fragments arranged by size: 890 361 258 117 BanII G_rGCy'C Cuts at: 0 730 1004 1417 1468 1626 Size: 730 274 413 51 158 Fragments arranged by size: 730 413 274 158 51 BbeI G_GCGC'C Cuts at: 0 740 1626 Size: 740 886 BbsI GAAGACnn'nnnn_ Cuts at: 0 147 750 1052 1079 1364 1626 Size: 147 603 302 27 285 262 Fragments arranged by size: 603 302 285 262 147 27 BbvCI CC'TCA_GC Cuts at: 0 1223 1389 1618 1626 Size: 1223 166 229 8 Fragments arranged by size: 1223 229 166 8 BclI T'GATC_A Cuts at: 0 1247 1626 Size: 1247 379 BlpI GC'TnA_GC Cuts at: 0 221 781 1626 Size: 221 560 845 Fragments arranged by size: 845 560 221 BmrI ACTGGGnnnn_n' Cuts at: 0 1133 1205 1626 Size: 1133 72 421 Fragments arranged by size: 1133 421 72 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 211 1590 1626 Size: 211 1379 36 Fragments arranged by size: 1379 211 36 Bpu10I CC'TnA_GC Cuts at: 0 1223 1389 1618 1626 Size: 1223 166 229 8 Fragments arranged by size: 1223 229 166 8 BsaBI GATnn'nnATC Cuts at: 0 949 1626 Size: 949 677 BsaHI Gr'CG_yC Cuts at: 0 329 737 827 1626 Size: 329 408 90 799 Fragments arranged by size: 799 408 329 90 BsaWI w'CCGG_w Cuts at: 0 595 881 1626 Size: 595 286 745 Fragments arranged by size: 745 595 286 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1269 1299 1626 Size: 1269 30 327 Fragments arranged by size: 1269 327 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 652 1059 1163 1587 1626 Size: 652 407 104 424 39 Fragments arranged by size: 652 424 407 104 39 BseYI C'CCAG_C Cuts at: 0 1049 1626 Size: 1049 577 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1113 1626 Size: 1113 513 BsiHKAI G_wGCw'C Cuts at: 0 730 782 1626 Size: 730 52 844 Fragments arranged by size: 844 730 52 BsmI GAATG_Cn' Cuts at: 0 545 1626 Size: 545 1081 BsmBI CGTCTCn'nnnn_ Cuts at: 0 316 818 1626 Size: 316 502 808 Fragments arranged by size: 808 502 316 Bsp1286I G_dGCh'C Cuts at: 0 730 782 1004 1417 1468 1626 Size: 730 52 222 413 51 158 Fragments arranged by size: 730 413 222 158 52 51 BspHI T'CATG_A Cuts at: 0 644 1250 1626 Size: 644 606 376 Fragments arranged by size: 644 606 376 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 735 744 1626 Size: 735 9 882 Fragments arranged by size: 882 735 9 BsrBI CCG'CTC Cuts at: 0 1407 1626 Size: 1407 219 BsrDI GCAATG_nn' Cuts at: 0 373 981 1626 Size: 373 608 645 Fragments arranged by size: 645 608 373 BsrFI r'CCGG_y Cuts at: 0 595 1183 1564 1626 Size: 595 588 381 62 Fragments arranged by size: 595 588 381 62 BsrGI T'GTAC_A Cuts at: 0 90 1626 Size: 90 1536 BstAPI GCAn_nnn'nTGC Cuts at: 0 201 1626 Size: 201 1425 DraIII CAC_nnn'GTG Cuts at: 0 216 1626 Size: 216 1410 DrdI GACnn_nn'nnGTC Cuts at: 0 151 1626 Size: 151 1475 EaeI y'GGCC_r Cuts at: 0 76 476 720 803 1017 1181 1379 1473 Size: 76 400 244 83 214 164 198 94 Cuts at: 1473 1626 Size: 153 Fragments arranged by size: 400 244 214 198 164 153 94 83 76 EarI CTCTTCn'nnn_ Cuts at: 0 27 1083 1626 Size: 27 1056 543 Fragments arranged by size: 1056 543 27 EcoICRI GAG'CTC Cuts at: 0 728 1626 Size: 728 898 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 35 211 311 1046 1590 1626 Size: 35 176 100 735 544 36 Fragments arranged by size: 735 544 176 100 36 35 EcoNI CCTnn'n_nnAGG Cuts at: 0 247 1626 Size: 247 1379 EcoO109I rG'GnC_Cy Cuts at: 0 423 693 809 1626 Size: 423 270 116 817 Fragments arranged by size: 817 423 270 116 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 774 1626 Size: 774 852 HaeII r_GCGC'y Cuts at: 0 740 1026 1168 1626 Size: 740 286 142 458 Fragments arranged by size: 740 458 286 142 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 991 1023 1404 1436 1626 Size: 991 32 381 32 190 Fragments arranged by size: 991 381 190 32 32 HincII GTy'rAC Cuts at: 0 303 1626 Size: 303 1323 KasI G'GCGC_C Cuts at: 0 736 1626 Size: 736 890 KpnI G_GTAC'C Cuts at: 0 262 1626 Size: 262 1364 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 154 1626 Size: 154 1472 MscI TGG'CCA Cuts at: 0 478 805 1019 1626 Size: 478 327 214 607 Fragments arranged by size: 607 478 327 214 MslI CAynn'nnrTG Cuts at: 0 745 1626 Size: 745 881 MspA1I CmG'CkG Cuts at: 0 186 340 662 801 1626 Size: 186 154 322 139 825 Fragments arranged by size: 825 322 186 154 139 NaeI GCC'GGC Cuts at: 0 1185 1626 Size: 1185 441 NarI GG'CG_CC Cuts at: 0 737 1626 Size: 737 889 NgoMIV G'CCGG_C Cuts at: 0 1183 1626 Size: 1183 443 NspI r_CATG'y Cuts at: 0 974 1371 1626 Size: 974 397 255 Fragments arranged by size: 974 397 255 PciI A'CATG_T Cuts at: 0 1367 1626 Size: 1367 259 PpuMI rG'GwC_Cy Cuts at: 0 423 693 809 1626 Size: 423 270 116 817 Fragments arranged by size: 817 423 270 116 PshAI GACnn'nnGTC Cuts at: 0 328 1626 Size: 328 1298 PstI C_TGCA'G Cuts at: 0 419 1626 Size: 419 1207 PvuII CAG'CTG Cuts at: 0 186 1626 Size: 186 1440 SacI G_AGCT'C Cuts at: 0 730 1626 Size: 730 896 ScaI AGT'ACT Cuts at: 0 837 1626 Size: 837 789 SexAI A'CCwGG_T Cuts at: 0 146 1270 1626 Size: 146 1124 356 Fragments arranged by size: 1124 356 146 SfcI C'TryA_G Cuts at: 0 415 490 1494 1626 Size: 415 75 1004 132 Fragments arranged by size: 1004 415 132 75 SfoI GGC'GCC Cuts at: 0 738 1626 Size: 738 888 SmlI C'TyrA_G Cuts at: 0 1410 1626 Size: 1410 216 SspI AAT'ATT Cuts at: 0 370 1626 Size: 370 1256 StyI C'CwwG_G Cuts at: 0 534 1296 1482 1626 Size: 534 762 186 144 Fragments arranged by size: 762 534 186 144 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1495 1626 Size: 1495 131 TatI w'GTAC_w Cuts at: 0 90 112 835 1626 Size: 90 22 723 791 Fragments arranged by size: 791 723 90 22 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1357 1389 1626 Size: 1357 32 237 Fragments arranged by size: 1357 237 32 Tth111I GACn'n_nGTC Cuts at: 0 1032 1626 Size: 1032 594 XhoI C'TCGA_G Cuts at: 0 1410 1626 Size: 1410 216 XmnI GAAnn'nnTTC Cuts at: 0 406 1626 Size: 406 1220 Enzymes that do cut: AarI Acc65I AcuI AfeI AflIII AgeI AlwNI ApoI AvaI AvrII BanI BanII BbeI BbsI BbvCI BclI BlpI BmrI BpmI Bpu10I BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PpuMI PshAI PstI PvuII SacI ScaI SexAI SfcI SfoI SmlI SspI StyI TaqII TatI TstI Tth111I XhoI XmnI Enzymes that do not cut: AatII AccI AclI AflII AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI BaeI BamHI BcgI BciVI BfrBI BglI BglII Bme1580I BmgBI BmtI BplI BpuEI BsaI BsaAI BsiEI BsiWI BspEI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI EagI EciI EcoRI EcoRV FseI FspI FspAI HindIII HpaI MfeI MluI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI XbaI XcmI XmaI ZraI