################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI AlwI AlwNI AocI ApaI ApoI AscI AseI AsnI Asp718I AspEI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MseI MspA1I MunI NaeI NcoI NdeI NgoMIV NheI NlaIII NotI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SfcI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tru9I Tsp509I Van91I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### Aor51HI 533 AGCGCT AGC^GCT Asp700I 375 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 289 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 54 CCCGGG C^[CT]CG[GA]G BbeI 441 GGCGCC GGCGC^C BbsI 295 GTCTTC GAAGAC(2/6) BpuAI 295 GTCTTC GAAGAC(2/6) BsoBI 54 CCCGGG C^[CT]CG[GA]G BsrFI 112 GCCGGT [GA]^CCGG[CT] BsrI 224 ACTGG ACTGG(1/-1) BstAPI 239 GCAAGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 131 CATCC GGATG(2/0) Cfr10I 112 GCCGGT [GA]^CCGG[CT] DraII 59 GGGACCC [GA]G^G[ACGT]CC[CT] EaeI 570 TGGCCG [CT]^GGCC[GA] Eco47III 533 AGCGCT AGC^GCT EcoNI 150 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 59 GGGACCC [GA]G^G[ACGT]CC[CT] FokI 131 CATCC GGATG(9/13) KasI 441 GGCGCC G^GCGCC MboII 296 TCTTC GAAGA(8/7) MslI 279 CACTGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 441 GGCGCC GG^CGCC NruI 411 TCGCGA TCG^CGA PflFI 289 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 592 GAGTC GAGTC(4/5) PpuMI 59 GGGACCC [GA]G^G[AT]CC[CT] SexAI 581 ACCTGGT A^CC[AT]GGT SfoI 441 GGCGCC GGC^GCC SmaI 54 CCCGGG CCC^GGG StuI 158 AGGCCT AGG^CCT Tth111I 289 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 54 CCCGGG C^CCGGG XmaI 54 CCCGGG C^CCGGG XmnI 375 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AccII 412 CGCG CG^CG 574 CGCG AciI 128 CCGC CCGC(-3/-1) 573 CCGC Alw44I 178 GTGCAC G^TGCAC 418 GTGCAC ApaLI 178 GTGCAC G^TGCAC 418 GTGCAC BpmI 151 CTGGAG CTGGAG(16/14) 358 CTGGAG BstUI 412 CGCG CG^CG 574 CGCG DpnII 255 GATC ^GATC 306 GATC HgaI 39 GACGC GACGC(5/10) 187 GACGC MaeII 269 ACGT A^CGT 284 ACGT MboI 255 GATC ^GATC 306 GATC MvnI 412 CGCG CG^CG 574 CGCG NdeII 255 GATC ^GATC 306 GATC SacI 139 GAGCTC GAGCT^C 364 GAGCTC Sau3AI 255 GATC ^GATC 306 GATC SfaNI 210 GATGC GCATC(5/9) 130 GCATC TfiI 100 GAATC G^A[AT]TC 380 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AcyI AluI AspHI AvaII BanI BanII BbvI BcnI BmyI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I DdeI EcoRII Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeIII MnlI MspI MvaI MwoI NciI NlaIV Sau96I ScrFI TaqI TseI Tsp45I TspRI TthHB8I