################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AgeI AhdI AocI ApaI AscI AseI AsnI AspEI AspI AvaI AviII AvrII BanII BbeI BbvCI BcnI BglI BlnI BlpI Bpu1102I BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspEI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I CpoI DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FseI FspI HapII HgaI Hin1I HindIII HpaII KasI KspI MluI MroI MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tsp45I Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AflII 1149 CTTAAG C^TTAAG Alw44I 81 GTGCAC G^TGCAC AlwNI 1805 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 552 AGCGCT AGC^GCT ApaLI 81 GTGCAC G^TGCAC Asp700I 1881 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 2635 TGGCCA TGG^CCA BamHI 2692 GGATCC G^GATCC BbrPI 1947 CACGTG CAC^GTG BcgI 873 CGATTCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2132 GTATCC GTATCC(6/5) BclI 2120 TGATCA T^GATCA BfrI 1149 CTTAAG C^TTAAG BglII 456 AGATCT A^GATCT BsaAI 1947 CACGTG [CT]AC^GT[GA] BsaBI 2142 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1987 GAGACC GGTCTC(1/5) BseRI 2557 GAGGAG GAGGAG(10/8) BsgI 363 CTGCAC GTGCAG(16/14) BsmI 746 GCATTC GAATGC(1/-1) Bsp1407I 2706 TGTACA T^GTACA BspDI 1771 ATCGAT AT^CGAT BspHI 2117 TCATGA T^CATGA BspLU11I 1571 ACATGT A^CATGT BspMI 1403 ACCTGC ACCTGC(4/8) BsrGI 2706 TGTACA T^GTACA BstBI 1375 TTCGAA TT^CGAA BstXI 2537 CCATTATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 1771 ATCGAT AT^CGAT DraIII 75 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 2635 TGGCCA [CT]^GGCC[GA] Eco47III 552 AGCGCT AGC^GCT EcoT14I 1430 CCTTGG C^C[AT][AT]GG FauI 2383 GCGGG CCCGC(4/6) FbaI 2120 TGATCA T^GATCA HaeII 552 AGCGCT [GA]GCGC^[CT] HpaI 2410 GTTAAC GTT^AAC MaeII 1948 ACGT A^CGT MamI 2142 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2635 TGGCCA TGG^CCA MscI 2635 TGGCCA TGG^CCA NspI 1571 ACATGT [GA]CATG^[CT] NspV 1375 TTCGAA TT^CGAA PmaCI 1947 CACGTG CAC^GTG PmlI 1947 CACGTG CAC^GTG PpuMI 699 GGGTCCC [GA]G^G[AT]CC[CT] RcaI 2117 TCATGA T^CATGA ScaI 1302 AGTACT AGT^ACT SfuI 1375 TTCGAA TT^CGAA SspBI 2706 TGTACA T^GTACA StyI 1430 CCTTGG C^C[AT][AT]GG XbaI 1362 TCTAGA T^CTAGA XcmI 257 CCATCTCAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1881 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Acc65I 132 GGTACC G^GTACC 974 GGTACC AciI 203 CCGC CCGC(-3/-1) 2383 GCGG AflIII 1571 ACATGT A^C[GA][CT]GT 1948 ACGTGT Asp718I 132 GGTACC G^GTACC 974 GGTACC AspHI 81 GTGCAC G[AT]GC[AT]^C 1072 GAGCAC BbsI 2338 GAAGAC GAAGAC(2/6) 1741 GTCTTC BpuAI 2338 GAAGAC GAAGAC(2/6) 1741 GTCTTC BsiHKAI 81 GTGCAC G[AT]GC[AT]^C 1072 GAGCAC CfoI 553 GCGC GCG^C 1926 GCGC DraII 35 GGGGCCT [GA]G^G[ACGT]CC[CT] 699 GGGTCCC EcoO109I 35 GGGGCCT [GA]G^G[ACGT]CC[CT] 699 GGGTCCC EcoRV 574 GATATC GAT^ATC 2187 GATATC HhaI 553 GCGC GCG^C 1926 GCGC HinP1I 553 GCGC G^CGC 1926 GCGC HincII 123 GTTGAC GT[CT]^[GA]AC 2410 GTTAAC HindII 123 GTTGAC GT[CT]^[GA]AC 2410 GTTAAC HphI 1498 TCACC GGTGA(8/7) 1686 TCACC KpnI 132 GGTACC GGTAC^C 974 GGTACC MfeI 656 CAATTG C^AATTG 2405 CAATTG MslI 156 CATAACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2044 CACTATCATG MunI 656 CAATTG C^AATTG 2405 CAATTG PleI 126 GACTC GAGTC(4/5) 1708 GAGTC PstI 108 CTGCAG CTGCA^G 1405 CTGCAG SfcI 108 CTGCAG C^T[GA][CT]AG 1405 CTGCAG SspI 902 AATATT AAT^ATT 1369 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI AvaII BanI BbvI BfaI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI EarI EcoRII Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SmlI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII