(Circular) (Six-base) MAPSORT of: pFN21AE3321.seq Check: 7597 from: 1 to: 7417 pFN21AE3321 7417 bp With 182 enzymes: SgfI * March 28, 2012 12:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5227 278 Size: 53 83 186 936 3691 2468 Fragments arranged by size: 3691 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4760 1937 Size: 2823 4594 Acc65I G'GTAC_C Cuts at: 2137 2979 4738 2137 Size: 842 1759 4816 Fragments arranged by size: 4816 1759 842 AclI AA'CG_TT Cuts at: 1563 5544 5917 1563 Size: 3981 373 3063 Fragments arranged by size: 3981 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2144 3839 5480 6864 7132 1632 Size: 512 1695 1641 1384 268 1917 Fragments arranged by size: 1917 1695 1641 1384 512 268 AfeI AGC'GCT Cuts at: 2559 2559 Size: 7417 AflII C'TTAA_G Cuts at: 820 1017 3154 820 Size: 197 2137 5083 Fragments arranged by size: 5083 2137 197 AflIII A'CryG_T Cuts at: 1203 3576 3953 4714 6316 7293 1203 Size: 2373 377 761 1602 977 1327 Fragments arranged by size: 2373 1602 1327 977 761 377 AgeI A'CCGG_T Cuts at: 6248 6248 Size: 7417 AhdI GACnn_n'nnGTC Cuts at: 6146 6146 Size: 7417 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7417 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7417 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2490 2522 2490 Size: 32 7385 AlwNI CAG_nnn'CTG Cuts at: 3815 5160 6732 3815 Size: 1345 1572 4500 Fragments arranged by size: 4500 1572 1345 ApaLI G'TGCA_C Cuts at: 2086 5474 6630 2086 Size: 3388 1156 2873 Fragments arranged by size: 3388 2873 1156 ApoI r'AATT_y Cuts at: 1493 2374 2446 2550 2986 3603 4358 4726 4952 Size: 881 72 104 436 617 755 368 226 Cuts at: 4952 5104 7213 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 881 755 617 436 368 226 152 104 72 AseI AT'TA_AT Cuts at: 160 1032 5971 160 Size: 872 4939 1606 Fragments arranged by size: 4939 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7417 AvaI C'yCGr_G Cuts at: 1870 1943 4742 1870 Size: 73 2799 4545 Fragments arranged by size: 4545 2799 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2121 2154 3192 3225 2121 Size: 33 1038 33 6313 Fragments arranged by size: 6313 1038 33 33 BamHI G'GATC_C Cuts at: 4697 4747 5114 4697 Size: 50 367 7000 Fragments arranged by size: 7000 367 50 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2137 2979 4664 4738 6193 Size: 325 217 627 350 842 1685 74 1455 Cuts at: 6193 618 Size: 1842 Fragments arranged by size: 1842 1685 1455 842 627 350 325 217 74 BanII G_rGCy'C Cuts at: 1391 4736 1391 Size: 3345 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3739 4350 928 Size: 971 1840 611 3995 Fragments arranged by size: 3995 1840 971 611 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2867 2901 5608 5642 1903 Size: 34 930 34 2707 34 3678 Fragments arranged by size: 3678 2707 930 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4148 5309 6519 4148 Size: 1161 1210 5046 Fragments arranged by size: 5046 1210 1161 BclI T'GATC_A Cuts at: 1548 4125 1548 Size: 2577 4840 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6028 136 Size: 107 122 71 631 605 4356 1525 Fragments arranged by size: 4356 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2461 7412 2461 Size: 4951 2466 BlpI GC'TnA_GC Cuts at: 4834 4834 Size: 7417 Bme1580I G_kGCm'C Cuts at: 1790 2090 4105 5478 6634 1790 Size: 300 2015 1373 1156 2573 Fragments arranged by size: 2573 2015 1373 1156 300 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7417 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3731 4058 5148 Size: 416 53 83 399 24 2314 327 1090 Cuts at: 5148 6106 442 Size: 958 1753 Fragments arranged by size: 2314 1753 1090 958 416 399 327 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7417 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2294 2306 2628 4207 6077 1239 Size: 1055 12 322 1579 1870 2579 Fragments arranged by size: 2579 1870 1579 1055 322 12 Bpu10I CC'TnA_GC Cuts at: 4011 4011 Size: 7417 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3611 4580 5542 6407 6705 3611 Size: 969 962 865 298 4323 Fragments arranged by size: 4323 969 962 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3986 6080 882 Size: 617 2487 2094 2219 Fragments arranged by size: 2487 2219 2094 617 BsaAI yAC'GTr Cuts at: 493 1204 3954 5208 493 Size: 711 2750 1254 2702 Fragments arranged by size: 2750 2702 1254 711 BsaBI GATnn'nnATC Cuts at: 4151 7411 4151 Size: 3260 4157 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5224 5606 6241 275 Size: 53 83 186 936 3691 382 635 1451 Fragments arranged by size: 3691 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5850 6248 6522 6669 7080 5850 Size: 398 274 147 411 6187 Fragments arranged by size: 6187 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3447 3477 1178 Size: 30 165 30 2044 30 5118 Fragments arranged by size: 5118 2044 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4577 1186 Size: 3391 4026 BseYI C'CCAG_C Cuts at: 2701 2708 6620 7116 2701 Size: 7 3912 496 3002 Fragments arranged by size: 3912 3002 496 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2353 1163 Size: 573 617 6227 Fragments arranged by size: 6227 617 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4867 5628 6656 1540 Size: 275 186 2866 761 1028 2301 Fragments arranged by size: 2866 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2090 3081 5478 5563 6634 2090 Size: 991 2397 85 1071 2873 Fragments arranged by size: 2873 2397 1071 991 85 BsmI GAATG_Cn' Cuts at: 2751 4928 5021 2751 Size: 2177 93 5147 Fragments arranged by size: 5147 2177 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 3081 4105 4736 5478 5563 6634 Size: 399 300 991 1024 631 742 85 1071 Cuts at: 6634 1391 Size: 2174 Fragments arranged by size: 2174 1071 1024 991 742 631 399 300 85 BspEI T'CCGG_A Cuts at: 7080 7080 Size: 7417 BspHI T'CATG_A Cuts at: 4122 5306 7036 4122 Size: 1184 1730 4503 Fragments arranged by size: 4503 1730 1184 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3417 4772 844 Size: 2573 1355 3489 Fragments arranged by size: 3489 2573 1355 BsrBI CCG'CTC Cuts at: 1394 1823 5304 1394 Size: 429 3481 3507 Fragments arranged by size: 3507 3481 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5912 6086 65 Size: 1183 4664 174 1396 Fragments arranged by size: 4664 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6061 6248 1700 Size: 110 4251 187 2869 Fragments arranged by size: 4251 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4711 96 Size: 4615 2802 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7417 BssSI C'ACGA_G Cuts at: 5477 6489 5477 Size: 1012 6405 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7417 BstBI TT'CG_AA Cuts at: 3381 6221 3381 Size: 2840 4577 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7417 BstXI CCAn_nnnn'nTGG Cuts at: 4549 4549 Size: 7417 BstYI r'GATC_y Cuts at: 1972 2461 3538 3732 4697 4747 5114 5499 5516 Size: 489 1077 194 965 50 367 385 17 Cuts at: 5516 6968 7054 7066 7412 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1077 965 489 385 367 346 194 86 50 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6865 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6678 Fragments arranged by size: 6678 683 56 BtsI GCAGTG_nn' Cuts at: 4624 4933 5727 5747 4624 Size: 309 794 20 6294 Fragments arranged by size: 6294 794 309 20 ClaI AT'CG_AT Cuts at: 1554 3777 1554 Size: 2223 5194 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4013 4048 529 Size: 35 3449 35 3898 Fragments arranged by size: 3898 3449 35 35 DraI TTT'AAA Cuts at: 2601 3798 4618 4721 2601 Size: 1197 820 103 5297 Fragments arranged by size: 5297 1197 820 103 DraIII CAC_nnn'GTG Cuts at: 2085 2085 Size: 7417 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6424 809 Size: 507 5108 1802 Fragments arranged by size: 5108 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4640 4864 5753 Size: 54 1425 50 275 18 2810 224 889 Cuts at: 5753 7382 8 Size: 1629 43 Fragments arranged by size: 2810 1629 1425 889 275 224 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4864 1537 Size: 275 3052 4090 Fragments arranged by size: 4090 3052 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3522 4333 4600 5347 1353 Size: 2169 811 267 747 3423 Fragments arranged by size: 3423 2169 811 747 267 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6004 6378 6524 387 Size: 1064 138 4415 374 146 1280 Fragments arranged by size: 4415 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2294 2306 2628 3839 4207 5480 Size: 393 512 150 12 322 1211 368 1273 Cuts at: 5480 6077 6864 7132 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1273 1211 787 597 512 393 368 322 268 150 12 EcoO109I rG'GnC_Cy Cuts at: 2041 2705 2041 Size: 664 6753 EcoRI G'AATT_C Cuts at: 4726 5104 4726 Size: 378 7039 EcoRV GAT'ATC Cuts at: 2581 4194 2581 Size: 1613 5804 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4709 4709 Size: 7417 FspI TGC'GCA Cuts at: 5923 5923 Size: 7417 HaeII r_GCGC'y Cuts at: 1678 2561 6564 1678 Size: 883 4003 2531 Fragments arranged by size: 4003 2531 883 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2603 2635 3269 3301 3447 Size: 32 135 32 1031 32 634 32 146 Cuts at: 3447 3479 4070 4102 6058 6090 6132 6164 1373 Size: 32 591 32 1956 32 42 32 2626 Fragments arranged by size: 2626 1956 1031 634 591 146 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2130 4417 4761 5007 669 Size: 988 281 192 2287 344 246 3079 Fragments arranged by size: 3079 2287 988 344 281 246 192 HindIII A'AGCT_T Cuts at: 748 748 Size: 7417 HpaI GTT'AAC Cuts at: 4417 5007 4417 Size: 590 6827 KpnI G_GTAC'C Cuts at: 2141 2983 4742 2141 Size: 842 1759 4816 Fragments arranged by size: 4816 1759 842 MfeI C'AATT_G Cuts at: 2661 4410 5016 2661 Size: 1749 606 5062 Fragments arranged by size: 5062 1749 606 MluI A'CGCG_T Cuts at: 7293 7293 Size: 7417 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2424 3189 3561 3741 6531 6715 1305 Size: 1119 765 372 180 2790 184 2007 Fragments arranged by size: 2790 2007 1119 765 372 184 180 MscI TGG'CCA Cuts at: 10 64 1489 1832 4642 10 Size: 54 1425 343 2810 2785 Fragments arranged by size: 2810 2785 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2165 4053 5375 5734 5893 518 Size: 648 999 1888 1322 359 159 2042 Fragments arranged by size: 2042 1888 1322 999 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2512 2701 2782 3415 4833 5510 6658 6903 Size: 906 189 81 633 1418 677 1148 245 Cuts at: 6903 1606 Size: 2120 Fragments arranged by size: 2120 1418 1148 906 677 633 245 189 81 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6865 NdeI CA'TA_TG Cuts at: 387 387 Size: 7417 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7417 NotI GC'GGCC_GC Cuts at: 4864 4864 Size: 7417 NspI r_CATG'y Cuts at: 1132 1229 3580 4718 4775 6320 1132 Size: 97 2351 1138 57 1545 2229 Fragments arranged by size: 2351 2229 1545 1138 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7417 PciI A'CATG_T Cuts at: 3576 4714 6316 3576 Size: 1138 1602 4677 Fragments arranged by size: 4677 1602 1138 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7417 PmeI GTTT'AAAC Cuts at: 4721 4721 Size: 7417 PmlI CAC'GTG Cuts at: 3954 3954 Size: 7417 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2376 2408 5486 5518 6999 7031 2376 Size: 32 3078 32 1481 32 2762 Fragments arranged by size: 3078 2762 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2705 2705 Size: 7417 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7417 PsiI TTA'TAA Cuts at: 4454 4578 4987 4454 Size: 124 409 6884 Fragments arranged by size: 6884 409 124 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4209 4241 4209 Size: 32 7385 PstI C_TGCA'G Cuts at: 830 2117 3414 4769 830 Size: 1287 1297 1355 3478 Fragments arranged by size: 3478 1355 1297 1287 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7417 PvuII CAG'CTG Cuts at: 2512 2701 2782 3415 2512 Size: 189 81 633 6514 Fragments arranged by size: 6514 633 189 81 SalI G'TCGA_C Cuts at: 1936 4759 1936 Size: 2823 4594 SanDI GG'GwC_CC Cuts at: 2705 2705 Size: 7417 SbfI CC_TGCA'GG Cuts at: 4769 4769 Size: 7417 ScaI AGT'ACT Cuts at: 3309 5665 7377 3309 Size: 2356 1712 3349 Fragments arranged by size: 3349 2356 1712 SfcI C'TryA_G Cuts at: 826 1045 2113 3410 4765 5900 6581 6772 826 Size: 219 1068 1297 1355 1135 681 191 1471 Fragments arranged by size: 1471 1355 1297 1135 1068 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7417 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7417 SmaI CCC'GGG Cuts at: 1872 4744 1872 Size: 2872 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3154 3590 4559 5521 6422 6684 Size: 197 926 1211 436 969 962 901 262 Cuts at: 6684 820 Size: 1553 Fragments arranged by size: 1553 1211 969 962 926 901 436 262 197 SnaBI TAC'GTA Cuts at: 493 5208 493 Size: 4715 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7417 SphI G_CATG'C Cuts at: 1132 4775 1132 Size: 3643 3774 SspI AAT'ATT Cuts at: 5 52 2909 3376 5341 5 Size: 47 2857 467 1965 2081 Fragments arranged by size: 2857 2081 1965 467 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7417 StyI C'CwwG_G Cuts at: 513 1065 3435 4856 513 Size: 552 2370 1421 3074 Fragments arranged by size: 3074 2370 1421 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5444 5461 5614 5799 6244 325 Size: 1255 3864 17 153 185 445 1498 Fragments arranged by size: 3864 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3307 4162 4216 Size: 274 80 33 51 1443 1330 855 54 Cuts at: 4216 4711 5663 7375 96 Size: 495 952 1712 138 Fragments arranged by size: 1712 1443 1330 952 855 495 274 138 80 54 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2050 2082 7377 7409 2050 Size: 32 5295 32 2058 Fragments arranged by size: 5295 2058 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7417 XbaI T'CTAG_A Cuts at: 3367 4753 3367 Size: 1386 6031 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2269 2269 Size: 7417 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7417 XmaI C'CCGG_G Cuts at: 1870 4742 1870 Size: 2872 4545 XmnI GAAnn'nnTTC Cuts at: 3890 5546 3890 Size: 1656 5761 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5225 276 Size: 53 83 186 936 3691 2468 Fragments arranged by size: 3691 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI