(Linear) (Six-base) MAPSORT of: KIEE3321.seq Check: 5016 from: 1 to: 2712 KIEE3321.orf With 182 enzymes: SgfI * March 22, 2012 16:29 .. Acc65I G'GTAC_C Cuts at: 0 132 974 2712 Size: 132 842 1738 Fragments arranged by size: 1738 842 132 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 1834 2712 Size: 139 1695 878 Fragments arranged by size: 1695 878 139 AfeI AGC'GCT Cuts at: 0 554 2712 Size: 554 2158 AflII C'TTAA_G Cuts at: 0 1149 2712 Size: 1149 1563 AflIII A'CryG_T Cuts at: 0 1571 1948 2712 Size: 1571 377 764 Fragments arranged by size: 1571 764 377 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 485 517 2712 Size: 485 32 2195 Fragments arranged by size: 2195 485 32 AlwNI CAG_nnn'CTG Cuts at: 0 1810 2712 Size: 1810 902 ApaLI G'TGCA_C Cuts at: 0 81 2712 Size: 81 2631 ApoI r'AATT_y Cuts at: 0 369 441 545 981 1598 2353 2712 Size: 369 72 104 436 617 755 359 Fragments arranged by size: 755 617 436 369 359 104 72 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 116 149 1187 1220 2712 Size: 116 33 1038 33 1492 Fragments arranged by size: 1492 1038 116 33 33 BamHI G'GATC_C Cuts at: 0 2692 2712 Size: 2692 20 BanI G'GyrC_C Cuts at: 0 132 974 2659 2712 Size: 132 842 1685 53 Fragments arranged by size: 1685 842 132 53 BbsI GAAGACnn'nnnn_ Cuts at: 0 1734 2345 2712 Size: 1734 611 367 Fragments arranged by size: 1734 611 367 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 862 896 2712 Size: 862 34 1816 Fragments arranged by size: 1816 862 34 BciVI GTATCCnnnnn_n' Cuts at: 0 2143 2712 Size: 2143 569 BclI T'GATC_A Cuts at: 0 2120 2712 Size: 2120 592 BglII A'GATC_T Cuts at: 0 456 2712 Size: 456 2256 Bme1580I G_kGCm'C Cuts at: 0 85 2100 2712 Size: 85 2015 612 Fragments arranged by size: 2015 612 85 BmrI ACTGGGnnnn_n' Cuts at: 0 1726 2053 2712 Size: 1726 327 659 Fragments arranged by size: 1726 659 327 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 289 301 623 2202 2712 Size: 289 12 322 1579 510 Fragments arranged by size: 1579 510 322 289 12 Bpu10I CC'TnA_GC Cuts at: 0 2006 2712 Size: 2006 706 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1606 2575 2712 Size: 1606 969 137 Fragments arranged by size: 1606 969 137 BsaI GGTCTCn'nnnn_ Cuts at: 0 1981 2712 Size: 1981 731 BsaAI yAC'GTr Cuts at: 0 1949 2712 Size: 1949 763 BsaBI GATnn'nnATC Cuts at: 0 2146 2712 Size: 2146 566 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1442 1472 2712 Size: 1442 30 1240 Fragments arranged by size: 1442 1240 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 2572 2712 Size: 2572 140 BseYI C'CCAG_C Cuts at: 0 696 703 2712 Size: 696 7 2009 Fragments arranged by size: 2009 696 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 348 2712 Size: 348 2364 BsiHKAI G_wGCw'C Cuts at: 0 85 1076 2712 Size: 85 991 1636 Fragments arranged by size: 1636 991 85 BsmI GAATG_Cn' Cuts at: 0 746 2712 Size: 746 1966 Bsp1286I G_dGCh'C Cuts at: 0 85 1076 2100 2712 Size: 85 991 1024 612 Fragments arranged by size: 1024 991 612 85 BspHI T'CATG_A Cuts at: 0 2117 2712 Size: 2117 595 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1412 2712 Size: 1412 1300 BsrGI T'GTAC_A Cuts at: 0 2706 2712 Size: 2706 6 BstBI TT'CG_AA Cuts at: 0 1376 2712 Size: 1376 1336 BstXI CCAn_nnnn'nTGG Cuts at: 0 2544 2712 Size: 2544 168 BstYI r'GATC_y Cuts at: 0 456 1533 1727 2692 2712 Size: 456 1077 194 965 20 Fragments arranged by size: 1077 965 456 194 20 BtsI GCAGTG_nn' Cuts at: 0 2619 2712 Size: 2619 93 ClaI AT'CG_AT Cuts at: 0 1772 2712 Size: 1772 940 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2008 2043 2712 Size: 2008 35 669 Fragments arranged by size: 2008 669 35 DraI TTT'AAA Cuts at: 0 596 1793 2613 2712 Size: 596 1197 820 99 Fragments arranged by size: 1197 820 596 99 DraIII CAC_nnn'GTG Cuts at: 0 80 2712 Size: 80 2632 EaeI y'GGCC_r Cuts at: 0 2635 2712 Size: 2635 77 EarI CTCTTCn'nnn_ Cuts at: 0 1517 2328 2595 2712 Size: 1517 811 267 117 Fragments arranged by size: 1517 811 267 117 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 289 301 623 1834 2202 2712 Size: 139 150 12 322 1211 368 510 Fragments arranged by size: 1211 510 368 322 150 139 12 EcoO109I rG'GnC_Cy Cuts at: 0 36 700 2712 Size: 36 664 2012 Fragments arranged by size: 2012 664 36 EcoRV GAT'ATC Cuts at: 0 576 2189 2712 Size: 576 1613 523 Fragments arranged by size: 1613 576 523 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2704 2712 Size: 2704 8 HaeII r_GCGC'y Cuts at: 0 556 2712 Size: 556 2156 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 598 630 1264 1296 1442 1474 2065 2097 Size: 598 32 634 32 146 32 591 32 Cuts at: 2097 2712 Size: 615 Fragments arranged by size: 634 615 598 591 146 32 32 32 32 HincII GTy'rAC Cuts at: 0 125 2412 2712 Size: 125 2287 300 Fragments arranged by size: 2287 300 125 HpaI GTT'AAC Cuts at: 0 2412 2712 Size: 2412 300 KpnI G_GTAC'C Cuts at: 0 136 978 2712 Size: 136 842 1734 Fragments arranged by size: 1734 842 136 MfeI C'AATT_G Cuts at: 0 656 2405 2712 Size: 656 1749 307 Fragments arranged by size: 1749 656 307 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 419 1184 1556 1736 2712 Size: 419 765 372 180 976 Fragments arranged by size: 976 765 419 372 180 MscI TGG'CCA Cuts at: 0 2637 2712 Size: 2637 75 MslI CAynn'nnrTG Cuts at: 0 160 2048 2712 Size: 160 1888 664 Fragments arranged by size: 1888 664 160 MspA1I CmG'CkG Cuts at: 0 507 696 777 1410 2712 Size: 507 189 81 633 1302 Fragments arranged by size: 1302 633 507 189 81 NspI r_CATG'y Cuts at: 0 1575 2712 Size: 1575 1137 PciI A'CATG_T Cuts at: 0 1571 2712 Size: 1571 1141 PmlI CAC'GTG Cuts at: 0 1949 2712 Size: 1949 763 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 371 403 2712 Size: 371 32 2309 Fragments arranged by size: 2309 371 32 PpuMI rG'GwC_Cy Cuts at: 0 700 2712 Size: 700 2012 PsiI TTA'TAA Cuts at: 0 2449 2573 2712 Size: 2449 124 139 Fragments arranged by size: 2449 139 124 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2204 2236 2712 Size: 2204 32 476 Fragments arranged by size: 2204 476 32 PstI C_TGCA'G Cuts at: 0 112 1409 2712 Size: 112 1297 1303 Fragments arranged by size: 1303 1297 112 PvuII CAG'CTG Cuts at: 0 507 696 777 1410 2712 Size: 507 189 81 633 1302 Fragments arranged by size: 1302 633 507 189 81 SanDI GG'GwC_CC Cuts at: 0 700 2712 Size: 700 2012 ScaI AGT'ACT Cuts at: 0 1304 2712 Size: 1304 1408 SfcI C'TryA_G Cuts at: 0 108 1405 2712 Size: 108 1297 1307 Fragments arranged by size: 1307 1297 108 SmlI C'TyrA_G Cuts at: 0 1149 1585 2554 2712 Size: 1149 436 969 158 Fragments arranged by size: 1149 969 436 158 SspI AAT'ATT Cuts at: 0 904 1371 2712 Size: 904 467 1341 Fragments arranged by size: 1341 904 467 StyI C'CwwG_G Cuts at: 0 1430 2712 Size: 1430 1282 TatI w'GTAC_w Cuts at: 0 1302 2157 2211 2706 2712 Size: 1302 855 54 495 6 Fragments arranged by size: 1302 855 495 54 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 45 77 2712 Size: 45 32 2635 Fragments arranged by size: 2635 45 32 XbaI T'CTAG_A Cuts at: 0 1362 2712 Size: 1362 1350 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 264 2712 Size: 264 2448 XmnI GAAnn'nnTTC Cuts at: 0 1885 2712 Size: 1885 827 Enzymes that do cut: Acc65I AcuI AfeI AflII AflIII AloI AlwNI ApaLI ApoI BaeI BamHI BanI BbsI BcgI BciVI BclI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrGI BstBI BstXI BstYI BtsI ClaI CspCI DraI DraIII EaeI EarI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII HpaI KpnI MfeI MmeI MscI MslI MspA1I NspI PciI PmlI PpiI PpuMI PsiI PsrI PstI PvuII SanDI ScaI SfcI SmlI SspI StyI TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII AccI AclI AgeI AhdI AleI AlfI ApaI AscI AseI AsiSI AvaI AvrII BanII BbeI BbvCI BfrBI BglI BlpI BmgBI BmtI BplI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII BstZ17I Bsu36I BtgI BtgZI DrdI EagI EciI EcoICRI EcoNI EcoRI FseI FspI FspAI HindIII KasI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PshAI PspOMI PspXI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TaqII Tth111I XhoI XmaI ZraI