Restriction map for
pFN21AE5354
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI Alw44I AlwNI AocI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanII BbvCI
BcgI BciVI BclI BcnI BfaI
BglI BglII BlnI BmyI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsiYI BslI BsmBI
BsoBI Bsp1286I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I Cac8I ClaI CpoI
DraII DraIII DsaI EaeI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoRV EcoT22I FauI
FbaI FseI FspI HaeIII HgaI
HindIII KpnI Ksp632I KspI MaeI
MflI MluI MluNI MroI MscI
MslI MspA1I NaeI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 235 GTAGAC GT^[AC][GT]AC
AccII 36 CGCG CG^CG
AcyI 85 GGCGCC G[GA]^CG[CT]C
AfaI 127 GTAC GT^AC
AflII 79 CTTAAG C^TTAAG
AluI 138 AGCT AG^CT
AlwI 621 GGATC GGATC(4/5)
Aor51HI 96 AGCGCT AGC^GCT
AseI 316 ATTAAT AT^TAAT
AsnI 316 ATTAAT AT^TAAT
AvaII 660 GGACC G^G[AT]CC
BanI 85 GGCGCC G^G[CT][GA]CC
BbeI 85 GGCGCC GGCGC^C
BbrPI 4 CACGTG CAC^GTG
BfrI 79 CTTAAG C^TTAAG
BlpI 488 GCTCAGC GC^T[ACGT]AGC
BpmI 275 CTGGAG CTGGAG(16/14)
Bpu1102I 488 GCTCAGC GC^T[ACGT]AGC
BsaAI 4 CACGTG [CT]AC^GT[GA]
BsaBI 106 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 85 GGCGCC G[GA]^CG[CT]C
BsmFI 600 GGGAC GGGAC(10/14)
BsmI 534 GAATGC GAATGC(1/-1)
Bsp1407I 126 TGTACA T^GTACA
BspMI 121 GCAGGT ACCTGC(4/8)
BsrGI 126 TGTACA T^GTACA
BstEII 27 GGTGACC G^GT[ACGT]ACC
BstPI 27 GGTGACC G^GT[ACGT]ACC
BstUI 36 CGCG CG^CG
CelII 488 GCTCAGC GC^T[ACGT]AGC
Cfr13I 660 GGACC G^G[ACGT]CC
DraI 711 TTTAAA TTT^AAA
DrdI 256 GACATTTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 96 AGCGCT AGC^GCT
EcoO65I 27 GGTGACC G^GT[ACGT]ACC
EcoRI 749 GAATTC G^AATTC
EcoT14I 89 CCAAGG C^C[AT][AT]GG
Hin1I 85 GGCGCC G[GA]^CG[CT]C
HincII 556 GTTAAC GT[CT]^[GA]AC
HindII 556 GTTAAC GT[CT]^[GA]AC
HpaI 556 GTTAAC GTT^AAC
KasI 85 GGCGCC G^GCGCC
MaeII 5 ACGT A^CGT
MaeIII 28 GTGAC ^GT[ACGT]AC
MamI 106 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 578 CAATTG C^AATTG
MunI 578 CAATTG C^AATTG
MvnI 36 CGCG CG^CG
NarI 85 GGCGCC GG^CGCC
PmaCI 4 CACGTG CAC^GTG
PmlI 4 CACGTG CAC^GTG
PshBI 316 ATTAAT AT^TAAT
RsaI 127 GTAC GT^AC
Sau96I 660 GGACC G^G[ACGT]CC
SfoI 85 GGCGCC GGC^GCC
SmlI 79 CTTAAG C^T[CT][GA]AG
SspBI 126 TGTACA T^GTACA
StyI 89 CCAAGG C^C[AT][AT]GG
Tsp45I 28 GTGAC ^GT[GC]AC
XcmI 89 CCAAGGTAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AciI 37 GCGG CCGC(-3/-1)
389 GCGG
AcsI 653 GAATTT [GA]^AATT[CT]
749 GAATTC
ApoI 653 GAATTT [GA]^AATT[CT]
749 GAATTC
BbsI 168 GAAGAC GAAGAC(2/6)
248 GAAGAC
BpuAI 168 GAAGAC GAAGAC(2/6)
248 GAAGAC
BsaJI 89 CCAAGG C^C[ACGT][ACGT]GG
143 CCCTGG
BsmAI 215 GAGAC GTCTC(1/5)
528 GAGAC
BsrFI 31 ACCGGC [GA]^CCGG[CT]
444 GCCGGT
BssKI 144 CCTGG ^CC[ACGT]GG
547 CCAGG
BstNI 144 CCTGG CC^[AT]GG
547 CCAGG
Cfr10I 31 ACCGGC [GA]^CCGG[CT]
444 GCCGGT
DpnII 622 GATC ^GATC
732 GATC
EcoRII 144 CCTGG ^CC[AT]GG
547 CCAGG
HapII 32 CCGG C^CGG
445 CCGG
HpaII 32 CCGG C^CGG
445 CCGG
HphI 27 GGTGA GGTGA(8/7)
382 GGTGA
MboI 622 GATC ^GATC
732 GATC
MspI 32 CCGG C^CGG
445 CCGG
MvaI 144 CCTGG CC^[AT]GG
547 CCAGG
NdeII 622 GATC ^GATC
732 GATC
PleI 171 GACTC GAGTC(4/5)
422 GACTC
Sau3AI 622 GATC ^GATC
732 GATC
ScrFI 144 CCTGG CC^[ACGT]GG
547 CCAGG
SfaNI 706 GATGC GCATC(5/9)
392 GCATC
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
BbvI BsrI BstF5I CfoI DdeI
Fnu4HI FokI HaeII HhaI HinP1I
HinfI ItaI MboII MnlI MseI
MwoI NlaIII NlaIV TfiI Tru9I
TseI Tsp509I TspRI