(Circular) (Six-base) MAPSORT of: pFN21AE5354.seq Check: 8679 from: 1 to: 5503 pFN21AE5354 5503 bp With 182 enzymes: SgfI * January 30, 2014 17:18 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2117 2117 Size: 5503 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3313 278 Size: 53 83 186 936 1777 2468 Fragments arranged by size: 2468 1777 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2241 2846 1937 Size: 304 605 4594 Fragments arranged by size: 4594 605 304 Acc65I G'GTAC_C Cuts at: 2824 2824 Size: 5503 AclI AA'CG_TT Cuts at: 1563 3630 4003 1563 Size: 2067 373 3063 Fragments arranged by size: 3063 2067 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3566 4950 5218 1632 Size: 1934 1384 268 1917 Fragments arranged by size: 1934 1917 1384 268 AfeI AGC'GCT Cuts at: 2103 2103 Size: 5503 AflII C'TTAA_G Cuts at: 820 1017 2084 820 Size: 197 1067 4239 Fragments arranged by size: 4239 1067 197 AflIII A'CryG_T Cuts at: 1203 4402 5379 1203 Size: 3199 977 1327 Fragments arranged by size: 3199 1327 977 AgeI A'CCGG_T Cuts at: 4334 4334 Size: 5503 AhdI GACnn_n'nnGTC Cuts at: 4232 4232 Size: 5503 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5503 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5503 AlwNI CAG_nnn'CTG Cuts at: 3246 4818 3246 Size: 1572 3931 ApaLI G'TGCA_C Cuts at: 3560 4716 3560 Size: 1156 4347 ApoI r'AATT_y Cuts at: 1493 2658 2754 2812 3038 3190 5299 1493 Size: 1165 96 58 226 152 2109 1697 Fragments arranged by size: 2109 1697 1165 226 152 96 58 AseI AT'TA_AT Cuts at: 160 1032 2322 4057 160 Size: 872 1290 1735 1606 Fragments arranged by size: 1735 1606 1290 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5503 AvaI C'yCGr_G Cuts at: 1870 1943 2828 1870 Size: 73 885 4545 Fragments arranged by size: 4545 885 73 BamHI G'GATC_C Cuts at: 2833 3200 2833 Size: 367 5136 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2090 2824 4279 618 Size: 325 217 627 303 734 1455 1842 Fragments arranged by size: 1842 1455 734 627 325 303 217 BanII G_rGCy'C Cuts at: 1391 2822 1391 Size: 1431 4072 BbeI G_GCGC'C Cuts at: 2094 2094 Size: 5503 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2180 2260 928 Size: 971 281 80 4171 Fragments arranged by size: 4171 971 281 80 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3694 3728 1903 Size: 34 50 34 1673 34 3678 Fragments arranged by size: 3678 1673 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3395 4605 3395 Size: 1210 4293 BclI T'GATC_A Cuts at: 1548 1548 Size: 5503 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4114 136 Size: 107 122 71 631 605 2442 1525 Fragments arranged by size: 2442 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5498 5498 Size: 5503 BlpI GC'TnA_GC Cuts at: 2494 2920 2494 Size: 426 5077 Bme1580I G_kGCm'C Cuts at: 1790 3564 4720 1790 Size: 1774 1156 2573 Fragments arranged by size: 2573 1774 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5503 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2311 3234 4192 Size: 416 53 83 399 24 894 923 958 Cuts at: 4192 442 Size: 1753 Fragments arranged by size: 1753 958 923 894 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5503 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2301 4163 1239 Size: 1062 1862 2579 Fragments arranged by size: 2579 1862 1062 Bpu10I CC'TnA_GC Cuts at: 2046 2046 Size: 5503 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3628 4493 4791 3628 Size: 865 298 4340 Fragments arranged by size: 4340 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4166 882 Size: 617 2667 2219 Fragments arranged by size: 2667 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2011 3294 493 Size: 711 807 1283 2702 Fragments arranged by size: 2702 1283 807 711 BsaBI GATnn'nnATC Cuts at: 2115 5497 2115 Size: 3382 2121 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2091 3310 3692 4327 Size: 53 83 186 936 558 1219 382 635 Cuts at: 4327 275 Size: 1451 Fragments arranged by size: 1451 1219 936 635 558 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3936 4334 4608 4755 5166 3936 Size: 398 274 147 411 4273 Fragments arranged by size: 4273 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5278 Fragments arranged by size: 5278 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5503 BseYI C'CCAG_C Cuts at: 4706 5202 4706 Size: 496 5007 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4930 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2953 3714 4742 1540 Size: 275 186 952 761 1028 2301 Fragments arranged by size: 2301 1028 952 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3564 3649 4720 3564 Size: 85 1071 4347 Fragments arranged by size: 4347 1071 85 BsmI GAATG_Cn' Cuts at: 2545 3014 3107 2545 Size: 469 93 4941 Fragments arranged by size: 4941 469 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2822 3564 3649 4720 1391 Size: 399 1032 742 85 1071 2174 Fragments arranged by size: 2174 1071 1032 742 399 85 BspEI T'CCGG_A Cuts at: 5166 5166 Size: 5503 BspHI T'CATG_A Cuts at: 3392 5122 3392 Size: 1730 3773 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2117 2858 844 Size: 1273 741 3489 Fragments arranged by size: 3489 1273 741 BsrBI CCG'CTC Cuts at: 1394 1823 3390 1394 Size: 429 1567 3507 Fragments arranged by size: 3507 1567 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3998 4172 65 Size: 1183 2750 174 1396 Fragments arranged by size: 2750 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2036 2449 4147 4334 1700 Size: 110 226 413 1698 187 2869 Fragments arranged by size: 2869 1698 413 226 187 110 BsrGI T'GTAC_A Cuts at: 96 2131 96 Size: 2035 3468 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5503 BssSI C'ACGA_G Cuts at: 3563 4575 3563 Size: 1012 4491 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5503 BstBI TT'CG_AA Cuts at: 4307 4307 Size: 5503 BstEII G'GTnAC_C Cuts at: 1184 2032 1184 Size: 848 4655 BstYI r'GATC_y Cuts at: 1972 2833 3200 3585 3602 5054 5140 5152 5498 Size: 861 367 385 17 1452 86 12 346 Cuts at: 5498 1972 Size: 1977 Fragments arranged by size: 1977 1452 861 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4951 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2208 487 Size: 683 56 982 3782 Fragments arranged by size: 3782 982 683 56 BtsI GCAGTG_nn' Cuts at: 3019 3813 3833 3019 Size: 794 20 4689 Fragments arranged by size: 4689 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5503 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5468 DraI TTT'AAA Cuts at: 2718 2807 2718 Size: 89 5414 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2267 4510 809 Size: 507 951 2243 1802 Fragments arranged by size: 2243 1802 951 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2950 3839 5468 Size: 54 1425 50 275 18 1120 889 1629 Cuts at: 5468 8 Size: 43 Fragments arranged by size: 1629 1425 1120 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2950 1537 Size: 275 1138 4090 Fragments arranged by size: 4090 1138 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3433 1353 Size: 2080 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4090 4464 4610 387 Size: 1064 138 2501 374 146 1280 Fragments arranged by size: 2501 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2301 3566 4163 4950 5218 1239 Size: 393 669 1265 597 787 268 1524 Fragments arranged by size: 1524 1265 787 669 597 393 268 EcoRI G'AATT_C Cuts at: 2754 2812 3190 2754 Size: 58 378 5067 Fragments arranged by size: 5067 378 58 FspI TGC'GCA Cuts at: 4009 4009 Size: 5503 HaeII r_GCGC'y Cuts at: 1678 2030 2078 2094 2105 4650 1678 Size: 352 48 16 11 2545 2531 Fragments arranged by size: 2545 2531 352 48 16 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4144 4176 4218 4250 1373 Size: 32 135 32 2572 32 42 32 2626 Fragments arranged by size: 2626 2572 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2563 2847 3093 669 Size: 988 281 625 284 246 3079 Fragments arranged by size: 3079 988 625 284 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5503 HpaI GTT'AAC Cuts at: 2563 3093 2563 Size: 530 4973 KasI G'GCGC_C Cuts at: 2090 2090 Size: 5503 KpnI G_GTAC'C Cuts at: 2828 2828 Size: 5503 MfeI C'AATT_G Cuts at: 2583 3102 2583 Size: 519 4984 MluI A'CGCG_T Cuts at: 5379 5379 Size: 5503 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2461 4617 4801 1305 Size: 1156 2156 184 2007 Fragments arranged by size: 2156 2007 1156 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3681 Fragments arranged by size: 3681 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3461 3820 3979 518 Size: 648 843 1452 359 159 2042 Fragments arranged by size: 2042 1452 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2919 3596 4744 4989 1606 Size: 1313 677 1148 245 2120 Fragments arranged by size: 2120 1313 1148 677 245 NarI GG'CG_CC Cuts at: 2091 2091 Size: 5503 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4951 NdeI CA'TA_TG Cuts at: 387 387 Size: 5503 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5503 NotI GC'GGCC_GC Cuts at: 2950 2950 Size: 5503 NspI r_CATG'y Cuts at: 1132 1229 2861 4406 1132 Size: 97 1632 1545 2229 Fragments arranged by size: 2229 1632 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5503 PciI A'CATG_T Cuts at: 4402 4402 Size: 5503 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5503 PmeI GTTT'AAAC Cuts at: 2807 2807 Size: 5503 PmlI CAC'GTG Cuts at: 2011 2011 Size: 5503 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3572 3604 5085 5117 3572 Size: 32 1481 32 3958 Fragments arranged by size: 3958 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5503 PsiI TTA'TAA Cuts at: 3073 3073 Size: 5503 PstI C_TGCA'G Cuts at: 830 2855 830 Size: 2025 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5503 SalI G'TCGA_C Cuts at: 1936 2845 1936 Size: 909 4594 SbfI CC_TGCA'GG Cuts at: 2855 2855 Size: 5503 ScaI AGT'ACT Cuts at: 3751 5463 3751 Size: 1712 3791 SfcI C'TryA_G Cuts at: 826 1045 2851 3986 4667 4858 826 Size: 219 1806 1135 681 191 1471 Fragments arranged by size: 1806 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2092 2092 Size: 5503 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5503 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5503 SmaI CCC'GGG Cuts at: 1872 2830 1872 Size: 958 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2084 3607 4508 4770 820 Size: 197 926 141 1523 901 262 1553 Fragments arranged by size: 1553 1523 926 901 262 197 141 SnaBI TAC'GTA Cuts at: 493 3294 493 Size: 2801 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5503 SphI G_CATG'C Cuts at: 1132 2861 1132 Size: 1729 3774 SspI AAT'ATT Cuts at: 5 52 3427 5 Size: 47 3375 2081 Fragments arranged by size: 3375 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5503 StyI C'CwwG_G Cuts at: 513 1065 2094 2942 513 Size: 552 1029 848 3074 Fragments arranged by size: 3074 1029 848 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3530 3547 3700 3885 4330 325 Size: 1255 1950 17 153 185 445 1498 Fragments arranged by size: 1950 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2131 3749 5461 Size: 274 80 33 51 1443 154 1618 1712 Cuts at: 5461 96 Size: 138 Fragments arranged by size: 1712 1618 1443 274 154 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5463 5495 5463 Size: 32 5471 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5503 XbaI T'CTAG_A Cuts at: 2839 2839 Size: 5503 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2101 2101 Size: 5503 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5503 XmaI C'CCGG_G Cuts at: 1870 2828 1870 Size: 958 4545 XmnI GAAnn'nnTTC Cuts at: 3632 3632 Size: 5503 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3311 276 Size: 53 83 186 936 1777 2468 Fragments arranged by size: 2468 1777 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI