Restriction map for
pFN21AE3986
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BclI BcnI BfrI
BglI BglII BlnI BmyI BpmI
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DpnII DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI Hin1I HincII HindII HpaI
HpaII KasI KpnI KspI MaeII
MamI MboI MfeI MflI MluI
MroI MspI MunI NaeI NarI
NciI NcoI NdeI NdeII NgoMIV
NheI NlaIV NotI NruI NsiI
NspI PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI Sau3AI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 880 CGCG CG^CG
AlwNI 34 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 205 ATTAAT AT^TAAT
AsnI 205 ATTAAT AT^TAAT
AvaII 297 GGACC G^G[AT]CC
BalI 942 TGGCCA TGG^CCA
BcgI 175 GCAAGGAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 482 GTATCC GTATCC(6/5)
BfaI 824 CTAG C^TAG
BlpI 231 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 231 GCTAAGC GC^T[ACGT]AGC
BsmI 258 GAATGC GAATGC(1/-1)
Bsp1407I 526 TGTACA T^GTACA
BsrDI 602 CATTGC GCAATG(2/0)
BsrGI 526 TGTACA T^GTACA
BssKI 486 CCTGG ^CC[ACGT]GG
BstBI 866 TTCGAA TT^CGAA
BstNI 486 CCTGG CC^[AT]GG
BstUI 880 CGCG CG^CG
CelII 231 GCTAAGC GC^T[ACGT]AGC
CfoI 881 GCGC GCG^C
Cfr13I 297 GGACC G^G[ACGT]CC
DraII 296 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 942 TGGCCA [CT]^GGCC[GA]
EarI 673 GAAGAG CTCTTC(1/4)
EcoO109I 296 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 486 CCTGG ^CC[AT]GG
HhaI 881 GCGC GCG^C
HinP1I 881 GCGC G^CGC
HindIII 234 AAGCTT A^AGCTT
Ksp632I 673 GAAGAG CTCTTC(1/4)
MaeI 824 CTAG C^TAG
MluNI 942 TGGCCA TGG^CCA
MscI 942 TGGCCA TGG^CCA
MvaI 486 CCTGG CC^[AT]GG
MvnI 880 CGCG CG^CG
MwoI 222 GCCTCCTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspV 866 TTCGAA TT^CGAA
PpuMI 296 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 205 ATTAAT AT^TAAT
SapI 673 GAAGAGC GCTCTTC(1/4)
Sau96I 297 GGACC G^G[ACGT]CC
ScrFI 486 CCTGG CC^[ACGT]GG
SfcI 54 CTACAG C^T[GA][CT]AG
SfuI 866 TTCGAA TT^CGAA
SspBI 526 TGTACA T^GTACA
SspI 892 AATATT AAT^ATT
Tsp45I 350 GTCAC ^GT[GC]AC
XcmI 476 CCAAGGGTATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 646 GAATTT [GA]^AATT[CT]
859 GAATTT
AfaI 527 GTAC GT^AC
784 GTAC
ApoI 646 GAATTT [GA]^AATT[CT]
859 GAATTT
Asp700I 646 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
859 GAATTTATTC
BsaJI 141 CCAAGG C^C[ACGT][ACGT]GG
476 CCAAGG
Cac8I 103 GCGAGC GC[ACGT]^[ACGT]GC
730 GCTTGC
EcoT14I 141 CCAAGG C^C[AT][AT]GG
476 CCAAGG
HaeIII 760 GGCC GG^CC
943 GGCC
HphI 74 TCACC GGTGA(8/7)
431 TCACC
MaeIII 350 GTCAC ^GT[ACGT]AC
598 GTAAC
MslI 658 CATATCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
916 CATCTTTATG
MspA1I 37 CAGCTG C[AC]G^C[GT]G
412 CAGCTG
PvuII 37 CAGCTG CAG^CTG
412 CAGCTG
RsaI 527 GTAC GT^AC
784 GTAC
SfaNI 772 GATGC GCATC(5/9)
915 GCATC
StyI 141 CCAAGG C^C[AT][AT]GG
476 CCAAGG
TaqI 184 TCGA T^CGA
867 TCGA
TthHB8I 184 TCGA T^CGA
867 TCGA
XmnI 646 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
859 GAATTTATTC
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AluI BbvI BsiYI BslI BstF5I
DdeI Fnu4HI FokI HinfI ItaI
MboII MnlI MseI NlaIII TfiI
Tru9I TseI Tsp509I TspRI