(Circular) (Six-base) MAPSORT of: pFN21AE1827.seq Check: 1189 from: 1 to: 5740 pFN21AE1827 5740 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3550 278 Size: 53 83 186 936 2014 2468 Fragments arranged by size: 2468 2014 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2162 3083 1937 Size: 225 921 4594 Fragments arranged by size: 4594 921 225 Acc65I G'GTAC_C Cuts at: 3061 3061 Size: 5740 AclI AA'CG_TT Cuts at: 1563 3867 4240 1563 Size: 2304 373 3063 Fragments arranged by size: 3063 2304 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2508 2523 2915 3803 5187 5455 1632 Size: 876 15 392 888 1384 268 1917 Fragments arranged by size: 1917 1384 888 876 392 268 15 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5543 AflIII A'CryG_T Cuts at: 1203 4639 5616 1203 Size: 3436 977 1327 Fragments arranged by size: 3436 1327 977 AgeI A'CCGG_T Cuts at: 2948 4571 2948 Size: 1623 4117 AhdI GACnn_n'nnGTC Cuts at: 4469 4469 Size: 5740 AleI CACnn'nnGTG Cuts at: 1166 2981 1166 Size: 1815 3925 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5740 AlwNI CAG_nnn'CTG Cuts at: 2513 3483 5055 2513 Size: 970 1572 3198 Fragments arranged by size: 3198 1572 970 ApaLI G'TGCA_C Cuts at: 3797 4953 3797 Size: 1156 4584 ApoI r'AATT_y Cuts at: 1493 2090 3049 3275 3427 5536 1493 Size: 597 959 226 152 2109 1697 Fragments arranged by size: 2109 1697 959 597 226 152 AseI AT'TA_AT Cuts at: 160 1032 4294 160 Size: 872 3262 1606 Fragments arranged by size: 3262 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 AvaI C'yCGr_G Cuts at: 1870 1943 3065 1870 Size: 73 1122 4545 Fragments arranged by size: 4545 1122 73 BamHI G'GATC_C Cuts at: 3070 3437 3070 Size: 367 5373 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3061 4516 618 Size: 325 217 627 1274 1455 1842 Fragments arranged by size: 1842 1455 1274 627 325 217 BanII G_rGCy'C Cuts at: 1391 3059 1391 Size: 1668 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2301 2601 928 Size: 971 402 300 4067 Fragments arranged by size: 4067 971 402 300 BbvCI CC'TCA_GC Cuts at: 2136 2136 Size: 5740 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3931 3965 1903 Size: 34 1994 34 3678 Fragments arranged by size: 3678 1994 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2253 3632 4842 2253 Size: 1379 1210 3151 Fragments arranged by size: 3151 1379 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5740 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2662 4351 136 Size: 107 122 71 631 605 990 1689 1525 Fragments arranged by size: 1689 1525 990 631 605 122 107 71 BglII A'GATC_T Cuts at: 2644 5735 2644 Size: 3091 2649 BlpI GC'TnA_GC Cuts at: 3157 3157 Size: 5740 Bme1580I G_kGCm'C Cuts at: 1790 3801 4957 1790 Size: 2011 1156 2573 Fragments arranged by size: 2573 2011 1156 BmgBI CAC'GTC Cuts at: 1318 2983 1318 Size: 1665 4075 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2340 3471 4429 Size: 416 53 83 399 24 923 1131 958 Cuts at: 4429 442 Size: 1753 Fragments arranged by size: 1753 1131 958 923 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5740 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3011 4400 1239 Size: 1772 1389 2579 Fragments arranged by size: 2579 1772 1389 Bpu10I CC'TnA_GC Cuts at: 2136 2136 Size: 5740 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3865 4730 5028 3865 Size: 865 298 4577 Fragments arranged by size: 4577 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2932 4403 882 Size: 617 1433 1471 2219 Fragments arranged by size: 2219 1471 1433 617 BsaAI yAC'GTr Cuts at: 493 1204 3531 493 Size: 711 2327 2702 Fragments arranged by size: 2702 2327 711 BsaBI GATnn'nnATC Cuts at: 2437 5734 2437 Size: 3297 2443 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3547 3929 4564 275 Size: 53 83 186 936 2014 382 635 1451 Fragments arranged by size: 2014 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2948 4173 4571 4845 4992 5403 2948 Size: 1225 398 274 147 411 3285 Fragments arranged by size: 3285 1225 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5515 Fragments arranged by size: 5515 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2747 3017 3051 1186 Size: 1561 270 34 3875 Fragments arranged by size: 3875 1561 270 34 BseYI C'CCAG_C Cuts at: 4943 5439 4943 Size: 496 5244 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2737 3040 1163 Size: 573 1001 303 3863 Fragments arranged by size: 3863 1001 573 303 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3190 3951 4979 1540 Size: 275 186 1189 761 1028 2301 Fragments arranged by size: 2301 1189 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2997 3032 3801 3886 4957 2997 Size: 35 769 85 1071 3780 Fragments arranged by size: 3780 1071 769 85 35 BsmI GAATG_Cn' Cuts at: 3251 3344 3251 Size: 93 5647 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2997 3032 3059 3801 3886 4957 1391 Size: 399 1207 35 27 742 85 1071 2174 Fragments arranged by size: 2174 1207 1071 742 399 85 35 27 BspEI T'CCGG_A Cuts at: 5403 5403 Size: 5740 BspHI T'CATG_A Cuts at: 3629 5359 3629 Size: 1730 4010 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2565 3095 844 Size: 1721 530 3489 Fragments arranged by size: 3489 1721 530 BsrBI CCG'CTC Cuts at: 1394 1823 3627 1394 Size: 429 1804 3507 Fragments arranged by size: 3507 1804 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2884 4235 4409 65 Size: 1183 1636 1351 174 1396 Fragments arranged by size: 1636 1396 1351 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2045 2948 4384 4571 1700 Size: 110 235 903 1436 187 2869 Fragments arranged by size: 2869 1436 903 235 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5740 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5740 BssSI C'ACGA_G Cuts at: 2921 3800 4812 2921 Size: 879 1012 3849 Fragments arranged by size: 3849 1012 879 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5740 BstBI TT'CG_AA Cuts at: 4544 4544 Size: 5740 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5740 BstXI CCAn_nnnn'nTGG Cuts at: 2625 2710 2625 Size: 85 5655 BstYI r'GATC_y Cuts at: 1972 2131 2644 2713 3070 3437 3822 3839 5291 Size: 159 513 69 357 367 385 17 1452 Cuts at: 5291 5377 5389 5735 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 513 385 367 357 346 159 86 69 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5188 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5001 Fragments arranged by size: 5001 683 56 BtsI GCAGTG_nn' Cuts at: 3256 4050 4070 3256 Size: 794 20 4926 Fragments arranged by size: 4926 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5740 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5705 DraI TTT'AAA Cuts at: 3044 3044 Size: 5740 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4747 809 Size: 507 3431 1802 Fragments arranged by size: 3431 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2047 2816 Size: 54 1425 50 275 18 213 4 769 Cuts at: 2816 3187 4076 5705 8 Size: 371 889 1629 43 Fragments arranged by size: 1629 1425 889 769 371 275 213 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 3187 1537 Size: 275 1375 4090 Fragments arranged by size: 4090 1375 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2018 3670 1353 Size: 665 1652 3423 Fragments arranged by size: 3423 1652 665 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2084 4327 4701 4847 387 Size: 1064 138 495 2243 374 146 1280 Fragments arranged by size: 2243 1280 1064 495 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2508 2523 2915 3011 3803 4400 5187 Size: 393 876 15 392 96 792 597 787 Cuts at: 5187 5455 1239 Size: 268 1524 Fragments arranged by size: 1524 876 792 787 597 393 392 268 96 15 EcoO109I rG'GnC_Cy Cuts at: 2926 2926 Size: 5740 EcoRI G'AATT_C Cuts at: 3049 3427 3049 Size: 378 5362 EcoRV GAT'ATC Cuts at: 2609 2609 Size: 5740 FseI GG_CCGG'CC Cuts at: 2049 2049 Size: 5740 FspI TGC'GCA Cuts at: 4246 4246 Size: 5740 HaeII r_GCGC'y Cuts at: 1678 2371 4887 1678 Size: 693 2516 2531 Fragments arranged by size: 2531 2516 693 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4381 4413 4455 4487 1373 Size: 32 135 32 2809 32 42 32 2626 Fragments arranged by size: 2809 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3084 3330 669 Size: 988 281 1146 246 3079 Fragments arranged by size: 3079 1146 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5740 HpaI GTT'AAC Cuts at: 3330 3330 Size: 5740 KpnI G_GTAC'C Cuts at: 3065 3065 Size: 5740 MfeI C'AATT_G Cuts at: 2872 3339 2872 Size: 467 5273 MluI A'CGCG_T Cuts at: 5616 5616 Size: 5740 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2100 2613 4854 5038 1305 Size: 795 513 2241 184 2007 Fragments arranged by size: 2241 2007 795 513 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2818 10 Size: 54 1425 343 986 2932 Fragments arranged by size: 2932 1425 986 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2492 2981 3698 4057 4216 518 Size: 648 1326 489 717 359 159 2042 Fragments arranged by size: 2042 1326 717 648 489 359 159 MspA1I CmG'CkG Cuts at: 1606 2751 3156 3833 4981 5226 1606 Size: 1145 405 677 1148 245 2120 Fragments arranged by size: 2120 1148 1145 677 405 245 NaeI GCC'GGC Cuts at: 2047 2047 Size: 5740 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5188 NdeI CA'TA_TG Cuts at: 387 2312 387 Size: 1925 3815 NgoMIV G'CCGG_C Cuts at: 2045 2045 Size: 5740 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5740 NotI GC'GGCC_GC Cuts at: 3187 3187 Size: 5740 NspI r_CATG'y Cuts at: 1132 1229 3098 4643 1132 Size: 97 1869 1545 2229 Fragments arranged by size: 2229 1869 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5740 PciI A'CATG_T Cuts at: 4639 4639 Size: 5740 PflMI CCAn_nnn'nTGG Cuts at: 1108 2814 1108 Size: 1706 4034 PmeI GTTT'AAAC Cuts at: 3044 3044 Size: 5740 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3809 3841 5322 5354 3809 Size: 32 1481 32 4195 Fragments arranged by size: 4195 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2926 2926 Size: 5740 PshAI GACnn'nnGTC Cuts at: 1613 2951 1613 Size: 1338 4402 PsiI TTA'TAA Cuts at: 3310 3310 Size: 5740 PstI C_TGCA'G Cuts at: 830 3092 830 Size: 2262 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5740 PvuII CAG'CTG Cuts at: 2751 2751 Size: 5740 SalI G'TCGA_C Cuts at: 1936 3082 1936 Size: 1146 4594 SbfI CC_TGCA'GG Cuts at: 3092 3092 Size: 5740 ScaI AGT'ACT Cuts at: 3988 5700 3988 Size: 1712 4028 SfcI C'TryA_G Cuts at: 826 1045 3088 4223 4904 5095 826 Size: 219 2043 1135 681 191 1471 Fragments arranged by size: 2043 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5740 SmaI CCC'GGG Cuts at: 1872 3067 1872 Size: 1195 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3844 4745 5007 820 Size: 197 926 1901 901 262 1553 Fragments arranged by size: 1901 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3531 493 Size: 3038 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5740 SphI G_CATG'C Cuts at: 1132 3098 1132 Size: 1966 3774 SspI AAT'ATT Cuts at: 5 52 2450 3664 5 Size: 47 2398 1214 2081 Fragments arranged by size: 2398 2081 1214 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5740 StyI C'CwwG_G Cuts at: 513 1065 2186 2589 3179 513 Size: 552 1121 403 590 3074 Fragments arranged by size: 3074 1121 590 552 403 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3767 3784 3937 4122 4567 325 Size: 1255 2187 17 153 185 445 1498 Fragments arranged by size: 2187 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2125 3986 5698 Size: 274 80 33 51 1443 148 1861 1712 Cuts at: 5698 96 Size: 138 Fragments arranged by size: 1861 1712 1443 274 148 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5700 5732 5700 Size: 32 5708 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5740 XbaI T'CTAG_A Cuts at: 3076 3076 Size: 5740 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5740 XmaI C'CCGG_G Cuts at: 1870 3065 1870 Size: 1195 4545 XmnI GAAnn'nnTTC Cuts at: 2451 3869 2451 Size: 1418 4322 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3548 276 Size: 53 83 186 936 2014 2468 Fragments arranged by size: 2468 2014 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI