Restriction map for
pFN21AE5424
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
CpoI DpnII DraII DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI MamI MboI MfeI
MflI MluI MluNI MroI MscI
MslI MunI NaeI NarI NcoI
NdeII NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SalI SapI Sau3AI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 27 enzymes) #
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AfaI 295 GTAC GT^AC
AluI 745 AGCT AG^CT
BbsI 457 GAAGAC GAAGAC(2/6)
BfaI 788 CTAG C^TAG
BglI 50 GCCGGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 339 CTGGAG CTGGAG(16/14)
BpuAI 457 GAAGAC GAAGAC(2/6)
BsmFI 344 GGGAC GGGAC(10/14)
BspDI 152 ATCGAT AT^CGAT
BsrDI 509 GCAATG GCAATG(2/0)
BstAPI 799 GCAAATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 152 ATCGAT AT^CGAT
DrdI 460 GACCTAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 97 TGGCCG [CT]^GGCC[GA]
EarI 232 GAAGAG CTCTTC(1/4)
HaeII 12 GGCGCT [GA]GCGC^[CT]
Ksp632I 232 GAAGAG CTCTTC(1/4)
KspI 100 CCGCGG CCGC^GG
MaeI 788 CTAG C^TAG
MaeII 5 ACGT A^CGT
MspA1I 100 CCGCGG C[AC]G^C[GT]G
NlaIV 104 GGAGCC GG[ACGT]^[ACGT]CC
PleI 70 GACTC GAGTC(4/5)
RsaI 295 GTAC GT^AC
SacII 100 CCGCGG CCGC^GG
TaqI 153 TCGA T^CGA
TthHB8I 153 TCGA T^CGA
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcsI 162 GAATTC [GA]^AATT[CT]
864 GAATTC
ApoI 162 GAATTC [GA]^AATT[CT]
864 GAATTC
BcnI 51 CCGGG CC^[GC]GG
132 CCGGG
BspMI 351 GCAGGT ACCTGC(4/8)
474 GCAGGT
BstF5I 306 CATCC GGATG(2/0)
391 GGATG
CfoI 13 GCGC GCG^C
77 GCGC
Cfr13I 398 GGGCC G^G[ACGT]CC
715 GGCCC
DdeI 285 CTCAG C^T[ACGT]AG
463 CTAAG
DraI 238 TTTAAA TTT^AAA
589 TTTAAA
EcoRI 162 GAATTC G^AATTC
864 GAATTC
FauI 74 CCCGC CCCGC(4/6)
9 GCGGG
FokI 306 CATCC GGATG(9/13)
391 GGATG
HapII 51 CCGG C^CGG
132 CCGG
HhaI 13 GCGC GCG^C
77 GCGC
HinP1I 13 GCGC G^CGC
77 GCGC
HpaII 51 CCGG C^CGG
132 CCGG
HphI 567 GGTGA GGTGA(8/7)
86 TCACC
MaeIII 358 GTGAC ^GT[ACGT]AC
674 GTGAC
MspI 51 CCGG C^CGG
132 CCGG
NciI 51 CCGGG CC^[GC]GG
132 CCGGG
NdeI 298 CATATG CA^TATG
662 CATATG
NlaIII 805 CATG CATG^
904 CATG
Sau96I 398 GGGCC G^G[ACGT]CC
715 GGCCC
Tsp45I 358 GTGAC ^GT[GC]AC
674 GTGAC
XcmI 375 CCAGCAGATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
612 CCATTACTTTTCTGG
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AccII AciI BbvI BsaJI BsiYI
BslI BsrI BssKI BstNI BstUI
Cac8I DsaI EcoRII Fnu4HI HaeIII
HinfI ItaI MboII MnlI MseI
MvaI MvnI MwoI ScrFI SfaNI
TfiI Tru9I TseI Tsp509I TspRI