(Circular) (Six-base) MAPSORT of: pFN21AE5424.seq Check: 8195 from: 1 to: 5668 pFN21AE5424 5668 bp With 182 enzymes: SgfI * January 30, 2014 17:19 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3478 278 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3011 1937 Size: 1074 4594 Acc65I G'GTAC_C Cuts at: 2989 2989 Size: 5668 AclI AA'CG_TT Cuts at: 1563 3795 4168 1563 Size: 2232 373 3063 Fragments arranged by size: 3063 2232 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2256 2392 2659 3731 5115 5383 1632 Size: 624 136 267 1072 1384 268 1917 Fragments arranged by size: 1917 1384 1072 624 268 267 136 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5471 AflIII A'CryG_T Cuts at: 1203 4567 5544 1203 Size: 3364 977 1327 Fragments arranged by size: 3364 1327 977 AgeI A'CCGG_T Cuts at: 4499 4499 Size: 5668 AhdI GACnn_n'nnGTC Cuts at: 4397 4397 Size: 5668 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5668 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5668 AlwNI CAG_nnn'CTG Cuts at: 3411 4983 3411 Size: 1572 4096 ApaLI G'TGCA_C Cuts at: 3725 4881 3725 Size: 1156 4512 ApoI r'AATT_y Cuts at: 1493 2167 2869 2977 3203 3355 5464 1493 Size: 674 702 108 226 152 2109 1697 Fragments arranged by size: 2109 1697 702 674 226 152 108 AseI AT'TA_AT Cuts at: 160 1032 4222 160 Size: 872 3190 1606 Fragments arranged by size: 3190 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 AvaI C'yCGr_G Cuts at: 1870 1943 2993 1870 Size: 73 1050 4545 Fragments arranged by size: 4545 1050 73 BamHI G'GATC_C Cuts at: 2998 3365 2998 Size: 367 5301 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2989 4444 618 Size: 325 217 627 1202 1455 1842 Fragments arranged by size: 1842 1455 1202 627 325 217 BanII G_rGCy'C Cuts at: 1391 2987 1391 Size: 1596 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2469 928 Size: 971 570 4127 Fragments arranged by size: 4127 971 570 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3859 3893 1903 Size: 34 1922 34 3678 Fragments arranged by size: 3678 1922 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3560 4770 3560 Size: 1210 4458 BclI T'GATC_A Cuts at: 1548 1548 Size: 5668 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2061 4279 136 Size: 107 122 71 631 605 389 2218 1525 Fragments arranged by size: 2218 1525 631 605 389 122 107 71 BglII A'GATC_T Cuts at: 5663 5663 Size: 5668 BlpI GC'TnA_GC Cuts at: 3085 3085 Size: 5668 Bme1580I G_kGCm'C Cuts at: 1790 3729 4885 1790 Size: 1939 1156 2573 Fragments arranged by size: 2573 1939 1156 BmgBI CAC'GTC Cuts at: 1318 2011 1318 Size: 693 4975 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2175 2835 3399 Size: 416 53 83 399 24 758 660 564 Cuts at: 3399 4357 442 Size: 958 1753 Fragments arranged by size: 1753 958 758 660 564 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5668 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2365 4328 1239 Size: 1126 1963 2579 Fragments arranged by size: 2579 1963 1126 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3793 4658 4956 3793 Size: 865 298 4505 Fragments arranged by size: 4505 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4331 882 Size: 617 2832 2219 Fragments arranged by size: 2832 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3459 493 Size: 711 2255 2702 Fragments arranged by size: 2702 2255 711 BsaBI GATnn'nnATC Cuts at: 5662 5662 Size: 5668 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3475 3857 4492 275 Size: 53 83 186 936 1942 382 635 1451 Fragments arranged by size: 1942 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4101 4499 4773 4920 5331 4101 Size: 398 274 147 411 4438 Fragments arranged by size: 4438 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5443 Fragments arranged by size: 5443 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5668 BseYI C'CCAG_C Cuts at: 2400 4871 5367 2400 Size: 2471 496 2701 Fragments arranged by size: 2701 2471 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5095 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3118 3879 4907 1540 Size: 275 186 1117 761 1028 2301 Fragments arranged by size: 2301 1117 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3729 3814 4885 3729 Size: 85 1071 4512 Fragments arranged by size: 4512 1071 85 BsmI GAATG_Cn' Cuts at: 3179 3272 3179 Size: 93 5575 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2987 3729 3814 4885 1391 Size: 399 1197 742 85 1071 2174 Fragments arranged by size: 2174 1197 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5331 5331 Size: 5668 BspHI T'CATG_A Cuts at: 3557 5287 3557 Size: 1730 3938 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2347 2470 3023 844 Size: 1503 123 553 3489 Fragments arranged by size: 3489 1503 553 123 BsrBI CCG'CTC Cuts at: 1394 1823 3555 1394 Size: 429 1732 3507 Fragments arranged by size: 3507 1732 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2521 4163 4337 65 Size: 1183 1273 1642 174 1396 Fragments arranged by size: 1642 1396 1273 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4312 4499 1700 Size: 110 2502 187 2869 Fragments arranged by size: 2869 2502 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5668 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5668 BssSI C'ACGA_G Cuts at: 3728 4740 3728 Size: 1012 4656 BstAPI GCAn_nnn'nTGC Cuts at: 794 2810 794 Size: 2016 3652 BstBI TT'CG_AA Cuts at: 4472 4472 Size: 5668 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5668 BstYI r'GATC_y Cuts at: 1972 2998 3365 3750 3767 5219 5305 5317 5663 Size: 1026 367 385 17 1452 86 12 346 Cuts at: 5663 1972 Size: 1977 Fragments arranged by size: 1977 1452 1026 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2024 2033 2105 513 Size: 552 939 20 9 72 4076 Fragments arranged by size: 4076 939 552 72 20 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2082 487 Size: 683 56 856 4073 Fragments arranged by size: 4073 856 683 56 BtsI GCAGTG_nn' Cuts at: 2430 2751 2785 3184 3978 3998 2430 Size: 321 34 399 794 20 4100 Fragments arranged by size: 4100 794 399 321 34 20 ClaI AT'CG_AT Cuts at: 1554 2158 1554 Size: 604 5064 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5633 DraI TTT'AAA Cuts at: 2245 2596 2972 2245 Size: 351 376 4941 Fragments arranged by size: 4941 376 351 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2471 4675 809 Size: 507 1155 2204 1802 Fragments arranged by size: 2204 1802 1155 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2102 3115 4004 Size: 54 1425 50 275 18 272 1013 889 Cuts at: 4004 5633 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1013 889 275 272 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3115 1537 Size: 275 1303 4090 Fragments arranged by size: 4090 1303 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2232 3598 1353 Size: 879 1366 3423 Fragments arranged by size: 3423 1366 879 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4255 4629 4775 387 Size: 1064 138 2666 374 146 1280 Fragments arranged by size: 2666 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2256 2365 2392 2659 3731 4328 5115 Size: 393 624 109 27 267 1072 597 787 Cuts at: 5115 5383 1239 Size: 268 1524 Fragments arranged by size: 1524 1072 787 624 597 393 268 267 109 27 EcoRI G'AATT_C Cuts at: 2167 2869 2977 3355 2167 Size: 702 108 378 4480 Fragments arranged by size: 4480 702 378 108 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2286 2726 2286 Size: 440 5228 FspI TGC'GCA Cuts at: 4174 4174 Size: 5668 HaeII r_GCGC'y Cuts at: 1678 2021 4815 1678 Size: 343 2794 2531 Fragments arranged by size: 2794 2531 343 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2060 2092 2634 2666 4309 Size: 32 135 32 488 32 542 32 1643 Cuts at: 4309 4341 4383 4415 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1643 542 488 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3012 3258 669 Size: 988 281 1074 246 3079 Fragments arranged by size: 3079 1074 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5668 HpaI GTT'AAC Cuts at: 3258 3258 Size: 5668 KpnI G_GTAC'C Cuts at: 2993 2993 Size: 5668 MfeI C'AATT_G Cuts at: 3267 3267 Size: 5668 MluI A'CGCG_T Cuts at: 5544 5544 Size: 5668 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4782 4966 1305 Size: 3477 184 2007 Fragments arranged by size: 3477 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3846 Fragments arranged by size: 3846 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3626 3985 4144 518 Size: 648 843 1617 359 159 2042 Fragments arranged by size: 2042 1617 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2107 3084 3761 4909 5154 1606 Size: 501 977 677 1148 245 2120 Fragments arranged by size: 2120 1148 977 677 501 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4177 Fragments arranged by size: 4177 939 552 NdeI CA'TA_TG Cuts at: 387 2304 2668 387 Size: 1917 364 3387 Fragments arranged by size: 3387 1917 364 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5668 NotI GC'GGCC_GC Cuts at: 3115 3115 Size: 5668 NspI r_CATG'y Cuts at: 1132 1229 3026 4571 1132 Size: 97 1797 1545 2229 Fragments arranged by size: 2229 1797 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5668 PciI A'CATG_T Cuts at: 4567 4567 Size: 5668 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5668 PfoI T'CCnGG_A Cuts at: 2136 2136 Size: 5668 PmeI GTTT'AAAC Cuts at: 2972 2972 Size: 5668 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3737 3769 5250 5282 3737 Size: 32 1481 32 4123 Fragments arranged by size: 4123 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5668 PsiI TTA'TAA Cuts at: 3238 3238 Size: 5668 PstI C_TGCA'G Cuts at: 830 3020 830 Size: 2190 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5668 SacII CC_GC'GG Cuts at: 2108 2108 Size: 5668 SalI G'TCGA_C Cuts at: 1936 3010 1936 Size: 1074 4594 SbfI CC_TGCA'GG Cuts at: 3020 3020 Size: 5668 ScaI AGT'ACT Cuts at: 3916 5628 3916 Size: 1712 3956 SfcI C'TryA_G Cuts at: 826 1045 3016 4151 4832 5023 826 Size: 219 1971 1135 681 191 1471 Fragments arranged by size: 1971 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5668 SmaI CCC'GGG Cuts at: 1872 2995 1872 Size: 1123 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3772 4673 4935 820 Size: 197 926 1829 901 262 1553 Fragments arranged by size: 1829 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3459 493 Size: 2966 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5668 SphI G_CATG'C Cuts at: 1132 3026 1132 Size: 1894 3774 SspI AAT'ATT Cuts at: 5 52 3592 5 Size: 47 3540 2081 Fragments arranged by size: 3540 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5668 StyI C'CwwG_G Cuts at: 513 1065 2004 3107 513 Size: 552 939 1103 3074 Fragments arranged by size: 3074 1103 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3695 3712 3865 4050 4495 325 Size: 1255 2115 17 153 185 445 1498 Fragments arranged by size: 2115 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2299 3914 5626 Size: 274 80 33 51 1443 322 1615 1712 Cuts at: 5626 96 Size: 138 Fragments arranged by size: 1712 1615 1443 322 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5628 5660 5628 Size: 32 5636 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5668 XbaI T'CTAG_A Cuts at: 3004 3004 Size: 5668 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2387 2624 2387 Size: 237 5431 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5668 XmaI C'CCGG_G Cuts at: 1870 2993 1870 Size: 1123 4545 XmnI GAAnn'nnTTC Cuts at: 3797 3797 Size: 5668 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3476 276 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI