(Circular) (Six-base) MAPSORT of: pFN21AB0468.seq Check: 2304 from: 1 to: 7657 pFN21AB0468 7657 bp With 182 enzymes: SgfI * December 28, 2011 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5467 278 Size: 53 83 186 936 3931 2468 Fragments arranged by size: 3931 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5000 1937 Size: 3063 4594 Acc65I G'GTAC_C Cuts at: 4978 4978 Size: 7657 AclI AA'CG_TT Cuts at: 1563 5784 6157 1563 Size: 4221 373 3063 Fragments arranged by size: 4221 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2055 2210 3010 3819 3968 4559 4880 5720 Size: 423 155 800 809 149 591 321 840 Cuts at: 5720 7104 7372 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 840 809 800 591 423 321 268 155 149 AfeI AGC'GCT Cuts at: 4203 4203 Size: 7657 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7460 AflIII A'CryG_T Cuts at: 1203 6556 7533 1203 Size: 5353 977 1327 Fragments arranged by size: 5353 1327 977 AgeI A'CCGG_T Cuts at: 6488 6488 Size: 7657 AhdI GACnn_n'nnGTC Cuts at: 3703 6386 3703 Size: 2683 4974 AleI CACnn'nnGTG Cuts at: 1166 2841 1166 Size: 1675 5982 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2772 4548 1184 Size: 1588 1776 4293 Fragments arranged by size: 4293 1776 1588 AlwNI CAG_nnn'CTG Cuts at: 2303 5400 6972 2303 Size: 3097 1572 2988 Fragments arranged by size: 3097 2988 1572 ApaLI G'TGCA_C Cuts at: 2678 5714 6870 2678 Size: 3036 1156 3465 Fragments arranged by size: 3465 3036 1156 ApoI r'AATT_y Cuts at: 1493 2429 3256 3417 3553 4009 4823 4966 5192 Size: 936 827 161 136 456 814 143 226 Cuts at: 5192 5344 7453 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 936 827 814 456 226 161 152 143 136 AseI AT'TA_AT Cuts at: 160 1032 6211 160 Size: 872 5179 1606 Fragments arranged by size: 5179 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7657 AvaI C'yCGr_G Cuts at: 1870 1943 4982 1870 Size: 73 3039 4545 Fragments arranged by size: 4545 3039 73 BamHI G'GATC_C Cuts at: 3056 4987 5354 3056 Size: 1931 367 5359 Fragments arranged by size: 5359 1931 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3044 3317 4978 6433 618 Size: 325 217 627 1257 273 1661 1455 1842 Fragments arranged by size: 1842 1661 1455 1257 627 325 273 217 BanII G_rGCy'C Cuts at: 1391 2516 2780 4976 1391 Size: 1125 264 2196 4072 Fragments arranged by size: 4072 2196 1125 264 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3986 928 Size: 971 2087 4599 Fragments arranged by size: 4599 2087 971 BbvCI CC'TCA_GC Cuts at: 4179 4179 Size: 7657 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5848 5882 1903 Size: 34 3911 34 3678 Fragments arranged by size: 3911 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3735 5549 6759 3735 Size: 1814 1210 4633 Fragments arranged by size: 4633 1814 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7657 BfrBI ATG'CAT Cuts at: 4525 4798 4525 Size: 273 7384 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6268 136 Size: 107 122 71 631 605 4596 1525 Fragments arranged by size: 4596 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7652 7652 Size: 7657 BlpI GC'TnA_GC Cuts at: 4318 5074 4318 Size: 756 6901 Bme1580I G_kGCm'C Cuts at: 1790 2682 3438 5718 6874 1790 Size: 892 756 2280 1156 2573 Fragments arranged by size: 2573 2280 1156 892 756 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7657 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3078 3467 5388 Size: 416 53 83 399 24 1661 389 1921 Cuts at: 5388 6346 442 Size: 958 1753 Fragments arranged by size: 1921 1753 1661 958 416 399 389 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2769 1056 Size: 1713 5944 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2725 2838 2845 4471 6317 1239 Size: 1486 113 7 1626 1846 2579 Fragments arranged by size: 2579 1846 1626 1486 113 7 Bpu10I CC'TnA_GC Cuts at: 4179 4179 Size: 7657 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2169 2506 2773 2905 3096 3576 4409 4465 5782 Size: 337 267 132 191 480 833 56 1317 Cuts at: 5782 6647 6945 2169 Size: 865 298 2881 Fragments arranged by size: 2881 1317 865 833 480 337 298 267 191 132 56 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2003 3618 6320 882 Size: 617 504 1615 2702 2219 Fragments arranged by size: 2702 2219 1615 617 504 BsaAI yAC'GTr Cuts at: 493 1204 5448 493 Size: 711 4244 2702 Fragments arranged by size: 4244 2702 711 BsaBI GATnn'nnATC Cuts at: 3723 7651 3723 Size: 3928 3729 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2529 5464 5846 6481 Size: 53 83 186 936 996 2935 382 635 Cuts at: 6481 275 Size: 1451 Fragments arranged by size: 2935 1451 996 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2159 3727 4950 6090 6488 6762 6909 7320 2159 Size: 1568 1223 1140 398 274 147 411 2496 Fragments arranged by size: 2496 1568 1223 1140 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7432 Fragments arranged by size: 7432 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2694 2772 4076 1186 Size: 1508 78 1304 4767 Fragments arranged by size: 4767 1508 1304 78 BseYI C'CCAG_C Cuts at: 6860 7356 6860 Size: 496 7161 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2742 1163 Size: 573 1006 6078 Fragments arranged by size: 6078 1006 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3617 5107 5868 6896 1540 Size: 275 186 1616 1490 761 1028 2301 Fragments arranged by size: 2301 1616 1490 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2516 2682 2930 3112 3592 4021 5718 5803 6874 Size: 166 248 182 480 429 1697 85 1071 Cuts at: 6874 2516 Size: 3299 Fragments arranged by size: 3299 1697 1071 480 429 248 182 166 85 BsmI GAATG_Cn' Cuts at: 3304 5168 5261 3304 Size: 1864 93 5700 Fragments arranged by size: 5700 1864 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2516 2682 2780 2930 3112 3438 3592 Size: 399 726 166 98 150 182 326 154 Cuts at: 3592 4021 4976 5718 5803 6874 1391 Size: 429 955 742 85 1071 2174 Fragments arranged by size: 2174 1071 955 742 726 429 399 326 182 166 154 150 98 85 BspEI T'CCGG_A Cuts at: 7320 7320 Size: 7657 BspHI T'CATG_A Cuts at: 2566 4617 5546 7276 2566 Size: 2051 929 1730 2947 Fragments arranged by size: 2947 2051 1730 929 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4824 5012 844 Size: 3980 188 3489 Fragments arranged by size: 3980 3489 188 BsrBI CCG'CTC Cuts at: 1394 1823 4939 5544 1394 Size: 429 3116 605 3507 Fragments arranged by size: 3507 3116 605 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2776 3065 4884 6152 6326 65 Size: 1183 1528 289 1819 1268 174 1396 Fragments arranged by size: 1819 1528 1396 1268 1183 289 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6301 6488 1700 Size: 110 4491 187 2869 Fragments arranged by size: 4491 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7657 BssHII G'CGCG_C Cuts at: 1925 2536 1925 Size: 611 7046 BssSI C'ACGA_G Cuts at: 4058 4197 5717 6729 4058 Size: 139 1520 1012 4986 Fragments arranged by size: 4986 1520 1012 139 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7657 BstBI TT'CG_AA Cuts at: 2945 6461 2945 Size: 3516 4141 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7657 BstXI CCAn_nnnn'nTGG Cuts at: 2992 4786 2992 Size: 1794 5863 BstYI r'GATC_y Cuts at: 1972 2250 2885 2960 2996 3056 3788 4987 5354 Size: 278 635 75 36 60 732 1199 367 Cuts at: 5354 5739 5756 7208 7294 7306 7652 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1199 732 635 385 367 346 278 86 75 60 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2316 513 Size: 552 939 312 5854 Fragments arranged by size: 5854 939 552 312 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4120 487 Size: 683 56 2894 4024 Fragments arranged by size: 4024 2894 683 56 BtsI GCAGTG_nn' Cuts at: 2836 2848 3033 3900 5173 5967 5987 2836 Size: 12 185 867 1273 794 20 4506 Fragments arranged by size: 4506 1273 867 794 185 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7657 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4866 4901 529 Size: 35 4302 35 3285 Fragments arranged by size: 4302 3285 35 35 DraI TTT'AAA Cuts at: 3328 4375 4961 3328 Size: 1047 586 6024 Fragments arranged by size: 6024 1047 586 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2061 6664 809 Size: 507 745 4603 1802 Fragments arranged by size: 4603 1802 745 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2446 3172 5104 Size: 54 1425 50 275 18 616 726 1932 Cuts at: 5104 5993 7622 8 Size: 889 1629 43 Fragments arranged by size: 1932 1629 1425 889 726 616 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5104 1537 Size: 275 3292 4090 Fragments arranged by size: 4090 3292 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4509 5587 1353 Size: 3156 1078 3423 Fragments arranged by size: 3423 3156 1078 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6244 6618 6764 387 Size: 1064 138 4655 374 146 1280 Fragments arranged by size: 4655 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2514 2514 Size: 7657 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2055 2210 2725 2838 2845 3010 3819 Size: 393 423 155 515 113 7 165 809 Cuts at: 3819 3968 4471 4559 4880 5720 6317 7104 7372 Size: 149 503 88 321 840 597 787 268 Cuts at: 7372 1239 Size: 1524 Fragments arranged by size: 1524 840 809 787 597 515 503 423 393 321 268 165 155 149 113 88 7 EcoO109I rG'GnC_Cy Cuts at: 2099 3118 2099 Size: 1019 6638 EcoRI G'AATT_C Cuts at: 3553 4823 4966 5344 3553 Size: 1270 143 378 5866 Fragments arranged by size: 5866 1270 378 143 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3783 3783 Size: 7657 FspI TGC'GCA Cuts at: 6163 6163 Size: 7657 HaeII r_GCGC'y Cuts at: 1678 4205 6804 1678 Size: 2527 2599 2531 Fragments arranged by size: 2599 2531 2527 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2267 2299 3574 3606 3689 Size: 32 135 32 695 32 1275 32 83 Cuts at: 3689 3721 4578 4610 6298 6330 6372 6404 1373 Size: 32 857 32 1688 32 42 32 2626 Fragments arranged by size: 2626 1688 1275 857 695 135 83 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2143 4162 5001 5247 669 Size: 988 281 205 2019 839 246 3079 Fragments arranged by size: 3079 2019 988 839 281 246 205 HindIII A'AGCT_T Cuts at: 748 748 Size: 7657 HpaI GTT'AAC Cuts at: 5247 5247 Size: 7657 KpnI G_GTAC'C Cuts at: 4982 4982 Size: 7657 MfeI C'AATT_G Cuts at: 5256 5256 Size: 7657 MluI A'CGCG_T Cuts at: 7533 7533 Size: 7657 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3362 3661 6771 6955 1305 Size: 2057 299 3110 184 2007 Fragments arranged by size: 3110 2057 2007 299 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2448 3174 10 Size: 54 1425 343 616 726 4493 Fragments arranged by size: 4493 1425 726 616 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2841 4616 5615 5974 6133 518 Size: 648 1675 1775 999 359 159 2042 Fragments arranged by size: 2042 1775 1675 999 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2422 3465 3870 4906 5073 5750 6898 7143 Size: 816 1043 405 1036 167 677 1148 245 Cuts at: 7143 1606 Size: 2120 Fragments arranged by size: 2120 1148 1043 1036 816 677 405 245 167 NcoI C'CATG_G Cuts at: 513 1065 2004 2316 513 Size: 552 939 312 5854 Fragments arranged by size: 5854 939 552 312 NdeI CA'TA_TG Cuts at: 387 387 Size: 7657 NheI G'CTAG_C Cuts at: 1052 2765 1052 Size: 1713 5944 NotI GC'GGCC_GC Cuts at: 5104 5104 Size: 7657 NsiI A_TGCA'T Cuts at: 4527 4800 4527 Size: 273 7384 NspI r_CATG'y Cuts at: 1132 1229 2715 2765 5015 6560 1132 Size: 97 1486 50 2250 1545 2229 Fragments arranged by size: 2250 2229 1545 1486 97 50 PasI CC'CwG_GG Cuts at: 1348 2095 2695 1348 Size: 747 600 6310 Fragments arranged by size: 6310 747 600 PciI A'CATG_T Cuts at: 6556 6556 Size: 7657 PflMI CCAn_nnn'nTGG Cuts at: 1108 4655 1108 Size: 3547 4110 PfoI T'CCnGG_A Cuts at: 4366 4366 Size: 7657 PmeI GTTT'AAAC Cuts at: 4961 4961 Size: 7657 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5726 5758 7239 7271 5726 Size: 32 1481 32 6112 Fragments arranged by size: 6112 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2099 3118 2099 Size: 1019 6638 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7657 PsiI TTA'TAA Cuts at: 5227 5227 Size: 7657 PstI C_TGCA'G Cuts at: 830 2843 4593 5009 830 Size: 2013 1750 416 3478 Fragments arranged by size: 3478 2013 1750 416 PvuI CG_AT'CG Cuts at: 2001 3617 2001 Size: 1616 6041 PvuII CAG'CTG Cuts at: 2422 3870 4906 2422 Size: 1448 1036 5173 Fragments arranged by size: 5173 1448 1036 SacI G_AGCT'C Cuts at: 2516 2516 Size: 7657 SalI G'TCGA_C Cuts at: 1936 4999 1936 Size: 3063 4594 SbfI CC_TGCA'GG Cuts at: 5009 5009 Size: 7657 ScaI AGT'ACT Cuts at: 2182 2989 5905 7617 2182 Size: 807 2916 1712 2222 Fragments arranged by size: 2916 2222 1712 807 SexAI A'CCwGG_T Cuts at: 4803 4803 Size: 7657 SfcI C'TryA_G Cuts at: 826 1045 2103 2304 2839 3004 3577 4589 5005 Size: 219 1058 201 535 165 573 1012 416 Cuts at: 5005 6140 6821 7012 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1058 1012 681 573 535 416 219 201 191 165 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7657 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7657 SmaI CCC'GGG Cuts at: 1872 4984 1872 Size: 3112 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2184 2485 2788 2920 3111 3591 Size: 197 926 241 301 303 132 191 480 Cuts at: 3591 4388 4444 5761 6662 6924 820 Size: 797 56 1317 901 262 1553 Fragments arranged by size: 1553 1317 926 901 797 480 303 301 262 241 197 191 132 56 SnaBI TAC'GTA Cuts at: 493 5448 493 Size: 4955 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7657 SphI G_CATG'C Cuts at: 1132 5015 1132 Size: 3883 3774 SspI AAT'ATT Cuts at: 5 52 2911 3184 5581 5 Size: 47 2859 273 2397 2081 Fragments arranged by size: 2859 2397 2081 273 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7657 StyI C'CwwG_G Cuts at: 513 1065 2004 2316 4911 5096 513 Size: 552 939 312 2595 185 3074 Fragments arranged by size: 3074 2595 939 552 312 185 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2129 2708 4147 5684 5701 5854 6039 Size: 1255 549 579 1439 1537 17 153 185 Cuts at: 6039 6484 325 Size: 445 1498 Fragments arranged by size: 1537 1498 1439 1255 579 549 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2180 2987 3062 Size: 274 80 33 51 1443 203 807 75 Cuts at: 3062 3192 5903 7615 96 Size: 130 2711 1712 138 Fragments arranged by size: 2711 1712 1443 807 274 203 138 130 80 75 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2042 2074 3417 3449 3459 3491 3632 3664 7617 Size: 32 1343 32 10 32 141 32 3953 Cuts at: 7617 7649 2042 Size: 32 2050 Fragments arranged by size: 3953 2050 1343 141 32 32 32 32 32 10 Tth111I GACn'n_nGTC Cuts at: 1316 4502 1316 Size: 3186 4471 XbaI T'CTAG_A Cuts at: 2041 3707 4993 2041 Size: 1666 1286 4705 Fragments arranged by size: 4705 1666 1286 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3581 4801 3581 Size: 1220 6437 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7657 XmaI C'CCGG_G Cuts at: 1870 4982 1870 Size: 3112 4545 XmnI GAAnn'nnTTC Cuts at: 3741 3778 5786 3741 Size: 37 2008 5612 Fragments arranged by size: 5612 2008 37 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5465 276 Size: 53 83 186 936 3931 2468 Fragments arranged by size: 3931 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI