################################## # NO CUT ENZYMES ( 184 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AfaI AflII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI KspI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NlaIV NotI NruI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsaI RsrII SacI SacII SalI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI StuI StyI SwaI Tsp45I Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 701 GTATAC GT^[AC][GT]AC AflIII 189 ACATGT A^C[GA][CT]GT Alw44I 119 GTGCAC G^TGCAC AlwI 481 GATCC GGATC(4/5) ApaLI 119 GTGCAC G^TGCAC Asp700I 715 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 119 GTGCAC G[AT]GC[AT]^C BbvI 93 GCAGC GCAGC(8/12) BcgI 33 GCACTTTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsiHKAI 119 GTGCAC G[AT]GC[AT]^C BspLU11I 189 ACATGT A^CATGT BssKI 697 CCTGG ^CC[ACGT]GG Bst1107I 701 GTATAC GTA^TAC BstNI 697 CCTGG CC^[AT]GG BstZ17I 701 GTATAC GTA^TAC DdeI 254 CTGAG C^T[ACGT]AG DpnII 481 GATC ^GATC EcoRII 697 CCTGG ^CC[AT]GG EcoT22I 279 ATGCAT ATGCA^T Fnu4HI 93 GCAGC GC^[ACGT]GC HaeIII 353 GGCC GG^CC HphI 475 GGTGA GGTGA(8/7) ItaI 93 GCAGC GC^[ACGT]GC MboI 481 GATC ^GATC MslI 282 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 319 CAGCTG C[AC]G^C[GT]G MvaI 697 CCTGG CC^[AT]GG MwoI 378 GCATGAAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeI 356 CATATG CA^TATG NdeII 481 GATC ^GATC NsiI 279 ATGCAT ATGCA^T NspI 189 ACATGT [GA]CATG^[CT] PflMI 446 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 298 CTGCAG CTGCA^G PvuII 319 CAGCTG CAG^CTG SapI 382 GAAGAGC GCTCTTC(1/4) Sau3AI 481 GATC ^GATC ScrFI 697 CCTGG CC^[ACGT]GG SfaNI 262 GATGC GCATC(5/9) SpeI 553 ACTAGT A^CTAGT TaqI 42 TCGA T^CGA TseI 93 GCAGC G^C[AT]GC TthHB8I 42 TCGA T^CGA Van91I 446 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 715 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AcsI 144 AAATTC [GA]^AATT[CT] 751 GAATTT ApoI 144 AAATTC [GA]^AATT[CT] 751 GAATTT BfaI 437 CTAG C^TAG 554 CTAG BmyI 31 GGGCAC G[AGT]GC[ACT]^C 119 GTGCAC Bsp1286I 31 GGGCAC G[AGT]GC[ACT]^C 119 GTGCAC BspHI 392 TCATGA T^CATGA 822 TCATGA DraI 271 TTTAAA TTT^AAA 870 TTTAAA EarI 382 GAAGAG CTCTTC(1/4) 657 GAAGAG HindIII 397 AAGCTT A^AGCTT 665 AAGCTT Ksp632I 382 GAAGAG CTCTTC(1/4) 657 GAAGAG MaeI 437 CTAG C^TAG 554 CTAG MnlI 345 GAGG CCTC(7/6) 500 GAGG RcaI 392 TCATGA T^CATGA 822 TCATGA SfcI 298 CTGCAG C^T[GA][CT]AG 373 CTACAG SspI 99 AATATT AAT^ATT 244 AATATT TspRI 451 CAGTG CA[GC]TG[ACGT][ACGT]^ 540 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 13 enzymes) # ######################################### AluI BsiYI BslI BsrI BstF5I FokI HinfI MboII MseI NlaIII TfiI Tru9I Tsp509I