################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI Asp700I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbvI BciVI BclI BcnI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiHKAI BsiWI BsmAI BsmBI Bsp1286I BspDI BspEI BspHI BspLU11I BspMI BsrFI BsrI BssHII BssKI BssSI Bst1107I BstBI BstEII BstF5I BstNI BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRII EcoRV EcoT22I FbaI FokI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HpaI HpaII HphI KasI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TseI Tth111I Van91I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### Acc65I 471 GGTACC G^GTACC AcsI 493 AAATTT [GA]^AATT[CT] AflII 595 CTTAAG C^TTAAG AlwI 249 GATCC GGATC(4/5) AocI 65 CCTCAGG CC^T[ACGT]AGG ApoI 493 AAATTT [GA]^AATT[CT] AseI 612 ATTAAT AT^TAAT AsnI 612 ATTAAT AT^TAAT Asp718I 471 GGTACC G^GTACC AvaI 25 CTCGAG C^[CT]CG[GA]G BanI 471 GGTACC G^G[CT][GA]CC BbsI 331 GTCTTC GAAGAC(2/6) BbvCI 4 GCTGAGG CCTCAGC(-5/-2) BcgI 31 GCAAGCTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 595 CTTAAG C^TTAAG BpuAI 331 GTCTTC GAAGAC(2/6) BseRI 7 GAGGAG GAGGAG(10/8) BsiEI 177 CGACCG CG[GA][CT]^CG BsmFI 260 GGGAC GGGAC(10/14) BsmI 391 GAATGC GAATGC(1/-1) BsoBI 25 CTCGAG C^[CT]CG[GA]G Bsp1407I 318 TGTACA T^GTACA BsrDI 670 GCAATG GCAATG(2/0) BsrGI 318 TGTACA T^GTACA BstAPI 134 GCAAGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 476 CCACAGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 248 AGATCC [GA]^GATC[CT] Bsu36I 65 CCTCAGG CC^T[ACGT]AGG Cfr13I 62 GGGCC G^G[ACGT]CC DraII 61 GGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 424 CCACGG C^C[GA][CT]GG EaeI 165 TGGCCG [CT]^GGCC[GA] Eco81I 65 CCTCAGG CC^T[ACGT]AGG EcoO109I 61 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoT14I 581 CCTTGG C^C[AT][AT]GG Fnu4HI 167 GCCGC GC^[ACGT]GC ItaI 167 GCCGC GC^[ACGT]GC KpnI 471 GGTACC GGTAC^C MaeIII 449 GTGAC ^GT[ACGT]AC MflI 248 AGATCC [GA]^GATC[CT] MspA1I 45 CAGCGG C[AC]G^C[GT]G NheI 408 GCTAGC G^CTAGC PaeR7I 25 CTCGAG C^TCGAG PshBI 612 ATTAAT AT^TAAT Sau96I 62 GGGCC G^G[ACGT]CC SfcI 309 CTACAG C^T[GA][CT]AG SspBI 318 TGTACA T^GTACA StyI 581 CCTTGG C^C[AT][AT]GG TaqI 26 TCGA T^CGA Tsp45I 449 GTGAC ^GT[GC]AC TspRI 53 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 26 TCGA T^CGA XcmI 191 CCACGATAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 25 CTCGAG C^TCGAG XhoII 248 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AfaI 319 GTAC GT^AC 472 GTAC BfaI 359 CTAG C^TAG 409 CTAG BsrBI 87 CCGCTC CCGCTC(-3/-3) 218 GAGCGG CfoI 161 GCGC GCG^C 240 GCGC DpnII 114 GATC ^GATC 249 GATC HhaI 161 GCGC GCG^C 240 GCGC HinP1I 161 GCGC G^CGC 240 GCGC HindIII 33 AAGCTT A^AGCTT 592 AAGCTT MaeI 359 CTAG C^TAG 409 CTAG MaeII 529 ACGT A^CGT 676 ACGT MboI 114 GATC ^GATC 249 GATC MseI 596 TTAA T^TAA 613 TTAA NdeII 114 GATC ^GATC 249 GATC NlaIV 61 GGGGCC GG[ACGT]^[ACGT]CC 471 GGTACC NspI 139 ACATGC [GA]CATG^[CT] 321 ACATGC PleI 121 GACTC GAGTC(4/5) 16 GAGTC RsaI 319 GTAC GT^AC 472 GTAC Sau3AI 114 GATC ^GATC 249 GATC SfaNI 95 GCATC GCATC(5/9) 242 GCATC TfiI 281 GATTC G^A[AT]TC 429 GATTC Tru9I 596 TTAA T^TAA 613 TTAA Tsp509I 494 AATT ^AATT 642 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AciI AluI BsaJI BsiYI BslI Cac8I DdeI FauI HaeIII HinfI MboII MnlI MwoI NlaIII SmlI