################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AflIII Alw44I ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BamHI BbrPI BbsI BbvCI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsiEI BsiWI BsmBI BspDI BspHI BspLU11I BssHII BssSI Bst1107I BstAPI BstBI BstZ17I CelII ClaI CpoI DraI DraII DraIII DrdI EagI EclXI Eco52I EcoO109I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI MamI MfeI MluI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tru9I Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AccIII 1097 TCCGGA T^CCGGA AcyI 163 GGCGCC G[GA]^CG[CT]C AgeI 205 ACCGGT A^CCGGT AhdI 1237 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1103 CCTCAGG CC^T[ACGT]AGG Aor51HI 253 AGCGCT AGC^GCT AspEI 1237 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 227 GTGCTC G[AT]GC[AT]^C AvaI 159 CTCGGG C^[CT]CG[GA]G BalI 794 TGGCCA TGG^CCA BanI 163 GGCGCC G^G[CT][GA]CC BanII 307 GAGCCC G[GA]GC[CT]^C BbeI 163 GGCGCC GGCGC^C BcgI 832 CGACAGTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 148 GCCTCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 163 GGCGCC G[GA]^CG[CT]C BseAI 1097 TCCGGA T^CCGGA BseRI 616 CTCCTC GAGGAG(10/8) BsiHKAI 227 GTGCTC G[AT]GC[AT]^C BsmI 337 GAATGC GAATGC(1/-1) BsoBI 159 CTCGGG C^[CT]CG[GA]G Bsp1407I 486 TGTACA T^GTACA BspEI 1097 TCCGGA T^CCGGA BspMI 920 ACCTGC ACCTGC(4/8) BsrBI 880 GAGCGG CCGCTC(-3/-3) BsrGI 486 TGTACA T^GTACA BsrI 871 ACTGG ACTGG(1/-1) Bsu36I 1103 CCTCAGG CC^T[ACGT]AGG EaeI 794 TGGCCA [CT]^GGCC[GA] Eam1105I 1237 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 991 GAAGAG CTCTTC(1/4) Eco47III 253 AGCGCT AGC^GCT Eco81I 1103 CCTCAGG CC^T[ACGT]AGG EcoNI 576 CCTCCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HgaI 46 GACGC GACGC(5/10) Hin1I 163 GGCGCC G[GA]^CG[CT]C HincII 742 GTCAAC GT[CT]^[GA]AC HindII 742 GTCAAC GT[CT]^[GA]AC KasI 163 GGCGCC G^GCGCC Ksp632I 991 GAAGAG CTCTTC(1/4) KspI 13 CCGCGG CCGC^GG MaeII 643 ACGT A^CGT MluNI 794 TGGCCA TGG^CCA MroI 1097 TCCGGA T^CCGGA MscI 794 TGGCCA TGG^CCA MspA1I 13 CCGCGG C[AC]G^C[GT]G NaeI 102 GCCGGC GCC^GGC NarI 163 GGCGCC GG^CGCC NcoI 197 CCATGG C^CATGG NgoMIV 102 GCCGGC G^CCGGC NspI 1149 ACATGC [GA]CATG^[CT] PinAI 205 ACCGGT A^CCGGT PstI 37 CTGCAG CTGCA^G SacII 13 CCGCGG CCGC^GG SexAI 788 ACCAGGT A^CC[AT]GGT SfiI 147 GGCCTCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 163 GGCGCC GGC^GCC SspBI 486 TGTACA T^GTACA SspI 754 AATATT AAT^ATT Tsp509I 1225 AATT ^AATT XbaI 672 TCTAGA T^CTAGA XcmI 1078 CCATACACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 623 GTAGAC GT^[AC][GT]AC 997 GTCTAC AlwNI 805 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG 916 CAGAACCTG BsaWI 205 ACCGGT [AT]^CCGG[AT] 1097 TCCGGA BsgI 352 CTGCAC GTGCAG(16/14) 1113 GTGCAG BsmAI 530 GTCTC GTCTC(1/5) 1245 GTCTC BsrDI 798 CATTGC GCAATG(2/0) 438 GCAATG BsrFI 102 GCCGGC [GA]^CCGG[CT] 205 ACCGGT BstEII 662 GGTCACC G^GT[ACGT]ACC 1211 GGTTACC BstPI 662 GGTCACC G^GT[ACGT]ACC 1211 GGTTACC BstXI 456 CCAAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 785 CCAACCAGGTGG BstYI 390 GGATCT [GA]^GATC[CT] 1094 AGATCC Cfr10I 102 GCCGGC [GA]^CCGG[CT] 205 ACCGGT DsaI 13 CCGCGG C^C[GA][CT]GG 197 CCATGG EcoO65I 662 GGTCACC G^GT[ACGT]ACC 1211 GGTTACC EcoT14I 197 CCATGG C^C[AT][AT]GG 936 CCAAGG MflI 390 GGATCT [GA]^GATC[CT] 1094 AGATCC MslI 506 CACTCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 685 CACAACAATG PflMI 579 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 653 CCAAACCCTGG PleI 218 GACTC GAGTC(4/5) 823 GAGTC SfcI 37 CTGCAG C^T[GA][CT]AG 222 CTATAG SmlI 331 CTTGAG C^T[CT][GA]AG 896 CTCAAG StyI 197 CCATGG C^C[AT][AT]GG 936 CCAAGG TaqI 888 TCGA T^CGA 1175 TCGA TthHB8I 888 TCGA T^CGA 1175 TCGA Van91I 579 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 653 CCAAACCCTGG XhoII 390 GGATCT [GA]^GATC[CT] 1094 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaII BbvI BcnI BfaI BmyI BpmI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TfiI TseI Tsp45I TspRI